BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0557.0084.p (442 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value L12942 [D] COG1196 Chromosome segregation ATPases 263 5e-70 YFL008w [D] COG1196 Chromosome segregation ATPases 115 1e-25 YLR086w [D] COG1196 Chromosome segregation ATPases 103 6e-22 SPBC29A10.04 [D] COG1196 Chromosome segregation ATPases 98 2e-20 ECU07g0680 [D] COG1196 Chromosome segregation ATPases 97 5e-20 >L12942 [D] COG1196 Chromosome segregation ATPases Length = 244 Score = 263 bits (671), Expect = 5e-70, Method: Compositional matrix adjust. Identities = 126/235 (53%), Positives = 172/235 (73%), Gaps = 1/235 (0%) Query: 201 LSDDYELSYELAKQRYAVPEDVPAAQMEVQRLKRSISSLGEVNLGAIEEYQRVHERFTFL 260 L+ +Y++S+E A+ + +D+ A+ +++ L+R I +LG +NL AI ++ V+ERFTFL Sbjct: 7 LNSEYQISFEEAQATAKIVDDLAEAEQKLKNLERQIKALGPINLDAIAQFDEVNERFTFL 66 Query: 261 SGQKDDLVEAKTTLYHVIREMEEEMSKRFKMTFDAIRREFGTVFTKLFGGGRADLMLLDP 320 S QKDDL+EAK L I +M +E+ RFK +FDAIR F T F ++F G ADL L Sbjct: 67 SSQKDDLLEAKNLLLSTIDDMNDEVKIRFKTSFDAIRESFKTTFAQMFVSGLADLEL-TS 125 Query: 321 EHMLDTGIDIVAQPPGKKLQNLQLLSGGERALTAMALLFAILQVKPVPFCVLDEVEAALD 380 +++L+ G++I QPPGKKL +L L+SGGE+ALTA+AL+FAIL+V+ VPF VLDEVEAALD Sbjct: 126 DNLLEAGVEIKVQPPGKKLSSLNLMSGGEKALTALALIFAILRVRTVPFVVLDEVEAALD 185 Query: 381 EANVVRFAQYLREFSEQTQFIVVTHRKGTMEEADVLYGVTMEEGGVSKLVSVKLE 435 EANV RF Y+ F QFIVVTHR+GTM A +YGVTM + GVSK++SVKL+ Sbjct: 186 EANVKRFGDYMNHFDNSNQFIVVTHRRGTMAAAGSMYGVTMADAGVSKMISVKLD 240 >YFL008w [D] COG1196 Chromosome segregation ATPases Length = 1225 Score = 115 bits (288), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 78/270 (28%), Positives = 142/270 (52%), Gaps = 16/270 (5%) Query: 182 STEVAVGRLDVE-LDNILRKLSDDYELSYELAKQRYAVPEDVPAAQMEV-QRLKRSISSL 239 S+E + L + DN +S+ +++Y+ ++Y + +A+ E+ Q++ L Sbjct: 954 SSETTIDDLPISSTDNEAITISNSIDINYKGLPKKYK-ENNTDSARKELEQKIHEVEEIL 1012 Query: 240 GEV--NLGAIEEYQRVHERFTFLSGQKDDLVEAKTTLYHVIREMEEEMSKRFKMTFDAIR 297 E+ N A+E Y RF ++ + + L + + + +++++ + F+ TFD + Sbjct: 1013 NELQPNARALERYDEAEGRFEVINNETEQLKAEEKKILNQFLKIKKKRKELFEKTFDYVS 1072 Query: 298 REFGTVFTKL---------FGGGRADLMLLDPEHMLDTGIDIVAQPPGKKLQNLQLLSGG 348 ++ +L GG A L + D + + GI A PP K+ ++++ LSGG Sbjct: 1073 DHLDAIYRELTKNPNSNVELAGGNASLTIEDEDEPFNAGIKYHATPPLKRFKDMEYLSGG 1132 Query: 349 ERALTAMALLFAILQVKPVPFCVLDEVEAALDEANVVRFAQYLREF-SEQTQFIVVTHRK 407 E+ + A+ALLFAI +P PF VLDEV+AALD NV R A Y+R + QFIV++ + Sbjct: 1133 EKTVAALALLFAINSYQPSPFFVLDEVDAALDITNVQRIAAYIRRHRNPDLQFIVISLKN 1192 Query: 408 GTMEEADVLYGVT-MEEGGVSKLVSVKLED 436 E++D L GV ++ SK++++ L + Sbjct: 1193 TMFEKSDALVGVYRQQQENSSKIITLDLSN 1222 >YLR086w [D] COG1196 Chromosome segregation ATPases Length = 1418 Score = 103 bits (257), Expect = 6e-22, Method: Compositional matrix adjust. Identities = 57/178 (32%), Positives = 95/178 (53%) Query: 242 VNLGAIEEYQRVHERFTFLSGQKDDLVEAKTTLYHVIREMEEEMSKRFKMTFDAIRREFG 301 V++G +EEY R F ++ V+ + + + ++++ F F+ I Sbjct: 1220 VDIGVLEEYARRLAEFKRRKLDLNNAVQKRDEVKEQLGILKKKRFDEFMAGFNIISMTLK 1279 Query: 302 TVFTKLFGGGRADLMLLDPEHMLDTGIDIVAQPPGKKLQNLQLLSGGERALTAMALLFAI 361 ++ + GG A+L L+D G+ PP K +N+ LSGGE+ L+++AL+FA+ Sbjct: 1280 EMYQMITMGGNAELELVDSLDPFSEGVTFSVMPPKKSWRNITNLSGGEKTLSSLALVFAL 1339 Query: 362 LQVKPVPFCVLDEVEAALDEANVVRFAQYLREFSEQTQFIVVTHRKGTMEEADVLYGV 419 + KP P V+DE++AALD NV A Y++E ++ QFIV++ R E A L GV Sbjct: 1340 HKYKPTPLYVMDEIDAALDFRNVSIVANYIKERTKNAQFIVISLRNNMFELAQQLVGV 1397 >SPBC29A10.04 [D] COG1196 Chromosome segregation ATPases Length = 1233 Score = 98.2 bits (243), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 62/202 (30%), Positives = 105/202 (51%), Gaps = 8/202 (3%) Query: 243 NLGAIEEYQRVHERFTFLSGQKDDLVEAKTTLYHVIREMEEEMSKRFKMTFDAIRREFGT 302 NL AIE + V R L + +A ++++ ++F+ F I + Sbjct: 1024 NLRAIERLETVETRLAKLDEEFAAARKAAKNAKERFNAVKQKRLQKFQAAFSHISEQIDP 1083 Query: 303 VFTKLFG------GGRADLMLLDPEHMLDTGIDIVAQPPGKKLQNLQLLSGGERALTAMA 356 ++ +L GG A L L D + GI A PP K+ +++ LSGGE+ + A+A Sbjct: 1084 IYKELTKSPAFPLGGTAYLTLDDLDEPYLGGIKFHAMPPMKRFRDMDQLSGGEKTMAALA 1143 Query: 357 LLFAILQVKPVPFCVLDEVEAALDEANVVRFAQYLREF-SEQTQFIVVTHRKGTMEEADV 415 LLFAI +P PF VLDE++AALD+ NV + A Y+R+ S QF+V++ + +++ Sbjct: 1144 LLFAIHSYQPSPFFVLDEIDAALDQTNVTKIANYIRQHASSGFQFVVISLKNQLFSKSEA 1203 Query: 416 LYGVTMEEG-GVSKLVSVKLED 436 L G+ ++ S+ +S+ + D Sbjct: 1204 LVGIYRDQQENSSRTLSINVRD 1225 >ECU07g0680 [D] COG1196 Chromosome segregation ATPases Length = 1112 Score = 97.1 bits (240), Expect = 5e-20, Method: Compositional matrix adjust. Identities = 44/117 (37%), Positives = 73/117 (62%) Query: 303 VFTKLFGGGRADLMLLDPEHMLDTGIDIVAQPPGKKLQNLQLLSGGERALTAMALLFAIL 362 ++ + GG A+L L+D G+ + PP K +++ LSGGE+ L+++AL+FA+ Sbjct: 976 IYKTITYGGNAELELVDHLDPFSEGVILSVMPPKKSWKSVGNLSGGEKTLSSLALIFALH 1035 Query: 363 QVKPVPFCVLDEVEAALDEANVVRFAQYLREFSEQTQFIVVTHRKGTMEEADVLYGV 419 + +P PF V+DE++AALD NV + ++RE SE QF+V++ R E ++ L GV Sbjct: 1036 KYRPSPFYVMDEIDAALDYRNVSVVSNFIREMSETAQFLVISLRSDMFELSETLLGV 1092 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.313 0.130 0.340 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,785,022 Number of extensions: 1058863 Number of successful extensions: 2350 Number of sequences better than 1.0e-05: 15 Number of HSP's gapped: 2406 Number of HSP's successfully gapped: 15 Length of query: 442 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 333 Effective length of database: 25,807,025 Effective search space: 8593739325 Effective search space used: 8593739325 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 117 (49.7 bits)