BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0557.0076.p (249 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_fabG [IQR] COG1028 Dehydrogenases with different specificitie... 311 8e-85 SA1074 [IQR] COG1028 Dehydrogenases with different specificities... 289 3e-78 lin1921 [IQR] COG1028 Dehydrogenases with different specificitie... 263 2e-70 VC2021 [IQR] COG1028 Dehydrogenases with different specificities... 243 1e-64 NMA0533 [IQR] COG1028 Dehydrogenases with different specificitie... 233 2e-61 >BS_fabG [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 246 Score = 311 bits (796), Expect = 8e-85, Method: Compositional matrix adjust. Identities = 150/245 (61%), Positives = 180/245 (73%) Query: 5 LKGQTALVTGGSRGIGRSIALQLAEHGVKVAVNYSGNEAAAQETVARIIELGSEAIAIRG 64 L +TA+VTG SRGIGRSIAL LA+ G V VNYSGNEA A E V I +G +AIA++ Sbjct: 2 LNDKTAIVTGASRGIGRSIALDLAKSGANVVVNYSGNEAKANEVVDEIKSMGRKAIAVKA 61 Query: 65 DVGNSQQAESMVKEVIDTWGKIDILVNNAGITRDNLIMRMKEEEFDQVIETNLKGVFNCL 124 DV N + ++M+KE + + IDILVNNAGITRDNLIMRMKE+E+D VI NLKGVFNC Sbjct: 62 DVSNPEDVQNMIKETLSVFSTIDILVNNAGITRDNLIMRMKEDEWDDVININLKGVFNCT 121 Query: 125 KSATRPMMKQRYGRXXXXXXXXXXXXXAGQLNYSAAKAGIIGMTKSAARELSSRGITVNC 184 K+ TR MMKQR GR GQ NY AAKAG+IG+TKS+A+EL+SR ITVN Sbjct: 122 KAVTRQMMKQRSGRIINVSSIVGVSGNPGQANYVAAKAGVIGLTKSSAKELASRNITVNA 181 Query: 185 IAPGFIDTEMTHQLSDEVRSEYVKGIPLARLGRPEDIAMTVVFLASEGAAYMTGQTLHVD 244 IAPGFI T+MT +L+ +V+ E +K IPLAR G P D++ V FLASEGA YMTGQTLH+D Sbjct: 182 IAPGFISTDMTDKLAKDVQDEMLKQIPLARFGEPSDVSSVVTFLASEGARYMTGQTLHID 241 Query: 245 GGMYM 249 GGM M Sbjct: 242 GGMVM 246 >SA1074 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 246 Score = 289 bits (740), Expect = 3e-78, Method: Compositional matrix adjust. Identities = 139/242 (57%), Positives = 182/242 (75%) Query: 8 QTALVTGGSRGIGRSIALQLAEHGVKVAVNYSGNEAAAQETVARIIELGSEAIAIRGDVG 67 ++ALVTG SRGIGRSIALQLAE G VAVNY+G++ A+ V I G ++ AI+ +V Sbjct: 5 KSALVTGASRGIGRSIALQLAEEGYNVAVNYAGSKEKAEAVVEEIKAKGVDSFAIQANVA 64 Query: 68 NSQQAESMVKEVIDTWGKIDILVNNAGITRDNLIMRMKEEEFDQVIETNLKGVFNCLKSA 127 ++ + ++M+KEV+ +G +D+LVNNAGITRDNL+MRMKE+E+D VI+TNLKGVFNC++ A Sbjct: 65 DADEVKAMIKEVVSQFGSLDVLVNNAGITRDNLLMRMKEQEWDDVIDTNLKGVFNCIQKA 124 Query: 128 TRPMMKQRYGRXXXXXXXXXXXXXAGQLNYSAAKAGIIGMTKSAARELSSRGITVNCIAP 187 T M++QR G GQ NY A KAG+IG+TKSAAREL+SRGITVN +AP Sbjct: 125 TPQMLRQRSGAIINLSSVVGAVGNPGQANYVATKAGVIGLTKSAARELASRGITVNAVAP 184 Query: 188 GFIDTEMTHQLSDEVRSEYVKGIPLARLGRPEDIAMTVVFLASEGAAYMTGQTLHVDGGM 247 GFI ++MT LSDE++ + + IPLAR G+ DIA TV FLAS+ A Y+TGQT+HV+GGM Sbjct: 185 GFIVSDMTDALSDELKEQMLTQIPLARFGQDTDIANTVAFLASDKAKYITGQTIHVNGGM 244 Query: 248 YM 249 YM Sbjct: 245 YM 246 >lin1921 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 200 Score = 263 bits (672), Expect = 2e-70, Method: Compositional matrix adjust. Identities = 0/245 (0%), Positives = 0/245 (0%) Query: 5 LKGQTALVTGGSRGIGRSIALQLAEHGVKVAVNYSGNEAAAQETVARIIELGSEAIAIRG 64 K G+ ++ A T+ + Sbjct: 3 DKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRAATIELGPLVRGLPHLRTI 62 Query: 65 DVGNSQQAESMVKEVIDTWGKIDILVNNAGITRDNLIMRMKEEEFDQVIETNLKGVFNCL 124 Q ++ + + + ++ ++ L+ Sbjct: 63 HRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNALMQDLEEEGYLVGLEKAKFVE 122 Query: 125 KSATRPMMKQRYGRXXXXXXXXXXXXXAGQLNYSAAKAGIIGMTKSAARELSSRGITVNC 184 + A ++ + G+ K A + + G + Sbjct: 123 RLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQLSWQGIEKEAWNQANQSGAMGDL 182 Query: 185 IAPGFIDTEMTHQLSDEVRSEYVKGIPLARLGRPEDIAMTVVFLASEGAAYMTGQTLHVD 244 Sbjct: 183 242 Query: 245 GGMYM 249 Sbjct: 243 247 >VC2021 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 248 Score = 243 bits (621), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 129/248 (52%), Positives = 169/248 (68%), Gaps = 4/248 (1%) Query: 2 FSALKGQTALVTGGSRGIGRSIALQLAEHGVKVAVNYSGNEAAAQETVARIIELGSEAIA 61 F L+G+ ALVTG SRGIG++IA LAE G KV + + +E+ AQ A LG Sbjct: 4 FMNLEGKVALVTGASRGIGKAIAELLAERGAKV-IGTATSESGAQ---AISDYLGDNGKG 59 Query: 62 IRGDVGNSQQAESMVKEVIDTWGKIDILVNNAGITRDNLIMRMKEEEFDQVIETNLKGVF 121 + +V N + E+++K + D +G +DILVNNAGITRDNL+MRMKEEE+ ++ETNL +F Sbjct: 60 MALNVTNPESIEAVLKAITDEFGGVDILVNNAGITRDNLLMRMKEEEWSDIMETNLTSIF 119 Query: 122 NCLKSATRPMMKQRYGRXXXXXXXXXXXXXAGQLNYSAAKAGIIGMTKSAARELSSRGIT 181 K+ R MMK+R GR AGQ NY+AAKAG+IG TKS ARE++SRG+T Sbjct: 120 RLSKAVLRGMMKKRQGRIINVGSVVGTMGNAGQANYAAAKAGVIGFTKSMAREVASRGVT 179 Query: 182 VNCIAPGFIDTEMTHQLSDEVRSEYVKGIPLARLGRPEDIAMTVVFLASEGAAYMTGQTL 241 VN +APGFI+T+MT L+DE R+ + +P RLG P +IA V FLAS AAY+TG+TL Sbjct: 180 VNTVAPGFIETDMTKALNDEQRTATLAQVPAGRLGDPREIASAVAFLASPEAAYITGETL 239 Query: 242 HVDGGMYM 249 HV+GGMYM Sbjct: 240 HVNGGMYM 247 >NMA0533 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 206 Score = 233 bits (595), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 0/245 (0%), Positives = 0/245 (0%), Gaps = 3/245 (1%) Query: 5 LKGQTALVTGGSRGIGRSIALQLAEHGVKVAVNYSGNEAAAQETVARIIELGSEAIAIRG 64 + S + ++ + + ++ + + + A Sbjct: 6 FTVLMVGLLVISVAGCADTDDTTPSTADEA-ISPTISANSSTDETTASPDNMTADRAK-- 62 Query: 65 DVGNSQQAESMVKEVIDTWGKIDILVNNAGITRDNLIMRMKEEEFDQVIETNLKGVFNCL 124 ++ S + T + + D L ++ Sbjct: 63 EIALSHAGLAEANVTFVTAKLDTEDGKQEYEIEFYSGNTEYDYDIDASTGEILSYDYDAE 122 Query: 125 KSATRPMMKQRYGRXXXXXXXXXXXXXAGQLNYSAAKAGIIGMTKSAARELSSRGITVNC 184 + + + + + + + + Sbjct: 123 NYSASGSTQSGDNSTYIGEEKAKSIALEKVSGATESDIRLYLDYEDGMAVYEGSIVFDSM 182 Query: 185 IAPGFIDTEMTHQLSDEVRSEYVKGIPLARLGRPEDIAMTVVFLASEGAAYMTGQTLHVD 244 Sbjct: 183 242 Query: 245 GGMYM 249 Sbjct: 243 247 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.132 0.364 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,722,191 Number of extensions: 554696 Number of successful extensions: 2840 Number of sequences better than 1.0e-05: 335 Number of HSP's gapped: 2256 Number of HSP's successfully gapped: 337 Length of query: 249 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 145 Effective length of database: 26,383,695 Effective search space: 3825635775 Effective search space used: 3825635775 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 114 (48.5 bits)