BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0557.0075.p (307 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_fabD [I] COG0331 (acyl-carrier-protein) S-malonyltransferase 325 6e-89 CAC3575 [I] COG0331 (acyl-carrier-protein) S-malonyltransferase 296 4e-80 YPO1598 [I] COG0331 (acyl-carrier-protein) S-malonyltransferase 228 7e-60 BS_fabD [I] COG0331 (acyl-carrier-protein) S-malonyltransferase 228 9e-60 PA2968 [I] COG0331 (acyl-carrier-protein) S-malonyltransferase 206 5e-53 >BS_fabD [I] COG0331 (acyl-carrier-protein) S-malonyltransferase Length = 317 Score = 325 bits (833), Expect = 6e-89, Method: Compositional matrix adjust. Identities = 159/304 (52%), Positives = 216/304 (71%) Query: 1 MGKIAFVFPGQGAQAVGMGKDVYDALPHSRAVFEKGDEVLGFPLSRLIFEGPDSELKQTV 60 M KIAF+FPGQG+Q +GMGK++Y+ +P ++ +F++ DE L LS LIFEG EL T Sbjct: 1 MSKIAFLFPGQGSQFIGMGKELYEQVPAAKRLFDEADETLETKLSSLIFEGDAEELTLTY 60 Query: 61 NTQPALVTTSAAYLEALRQHGVNPDYVAGHSLGEYSALVAAGALAYEEAVSLVRLRGRFM 120 N QPAL+TTS A LE ++ G+ PD+ AGHSLGEYSALVAAGAL++++AV VR RG FM Sbjct: 61 NAQPALLTTSIAVLEKFKESGITPDFTAGHSLGEYSALVAAGALSFKDAVYTVRKRGEFM 120 Query: 121 EEAVPGGQGAMAAVLGAEREALAELCRSISEAGNSVELANVNCPGQIVISGSKAGVEEAA 180 EAVP G+GAMAA+LG + EAL ++ ++E GN V+LAN+NCPGQIVISG+ GVE A+ Sbjct: 121 NEAVPAGEGAMAAILGMDAEALKQVTDKVTEEGNLVQLANLNCPGQIVISGTAKGVELAS 180 Query: 181 QRIKEIGGKRAIPLEVSGPFHSSLMKEAAERLAAELGKXXXXXXXXXXXXXXXXXXXXDP 240 + KE G KRAIPLEVSGPFHS LMK AAE+L L + Sbjct: 181 ELAKENGAKRAIPLEVSGPFHSELMKPAAEKLKEVLDACDIKDADVPVISNVSADVMTEK 240 Query: 241 DEIRELLMQQVFSPVLWQDSVEWLIAAGVDTFVEIGPGSVLAGLIRKIDKTVKVVNINSL 300 +I+E L++Q++SPV +++S+ LIA GV TF+EIGPG VL+GL++K+++ +K + ++ Sbjct: 241 ADIKEKLIEQLYSPVRFEESINKLIAEGVTTFIEIGPGKVLSGLVKKVNRRLKTIAVSDP 300 Query: 301 ESAE 304 E+ E Sbjct: 301 ETIE 304 >CAC3575 [I] COG0331 (acyl-carrier-protein) S-malonyltransferase Length = 308 Score = 296 bits (757), Expect = 4e-80, Method: Compositional matrix adjust. Identities = 148/303 (48%), Positives = 198/303 (65%), Gaps = 2/303 (0%) Query: 1 MGKIAFVFPGQGAQAVGMGKDVYDALPHSRAVFEKGDEVLGFPLSRLIFEGPDSELKQTV 60 MGKIAFVF GQG+Q VGMGKD+YD ++ F+K DEVLGF +S L FEG D EL T Sbjct: 1 MGKIAFVFSGQGSQYVGMGKDLYDNYQSAKETFDKADEVLGFKISELCFEGKDEELNLTE 60 Query: 61 NTQPALVTTSAAYLEALRQH-GVNPDYVAGHSLGEYSALVAAGALAYEEAVSLVRLRGRF 119 NTQPA++ TS A L AL + G+ PD VAG SLGEYSA V +G+ ++E+AV LV+ RGR+ Sbjct: 61 NTQPAVLITSIAALRALEEEKGIKPDVVAGLSLGEYSAHVCSGSFSFEDAVRLVKKRGRY 120 Query: 120 MEEAVPGGQGAMAAVLGAEREALAELCRSISEAGNSVELANVNCPGQIVISGSKAGVEEA 179 M+EAVP G G MAA++G E + + +C S+ G VE+AN NCPGQIVI+G VE A Sbjct: 121 MQEAVPKGIGTMAAIIGLEGDVVRGICAEQSKEG-IVEVANYNCPGQIVIAGEVKAVESA 179 Query: 180 AQRIKEIGGKRAIPLEVSGPFHSSLMKEAAERLAAELGKXXXXXXXXXXXXXXXXXXXXD 239 ++KE G +R + L VSGPFH+S++K AAE+L EL D Sbjct: 180 CTKLKESGARRTVMLSVSGPFHTSMLKSAAEKLEEELKNINIEDMKVPVITNVTGDYVED 239 Query: 240 PDEIRELLMQQVFSPVLWQDSVEWLIAAGVDTFVEIGPGSVLAGLIRKIDKTVKVVNINS 299 D+I+ LL +QV S V W+D++ ++ GVDTF+E+GPG L+ I+KI++ + + NI Sbjct: 240 KDQIKGLLKKQVMSSVRWEDTIRRMMDDGVDTFIELGPGKTLSSFIKKINRKMTIFNIEK 299 Query: 300 LES 302 E Sbjct: 300 AED 302 >YPO1598 [I] COG0331 (acyl-carrier-protein) S-malonyltransferase Length = 309 Score = 228 bits (582), Expect = 7e-60, Method: Compositional matrix adjust. Identities = 133/301 (44%), Positives = 180/301 (59%), Gaps = 2/301 (0%) Query: 1 MGKIAFVFPGQGAQAVGMGKDVYDALPHSRAVFEKGDEVLGFPLSRLIFEGPDSELKQTV 60 M K A VFPGQG+Q++GM D+ P A F + VLG+ L +L+ +GP EL +T Sbjct: 1 MSKFAMVFPGQGSQSLGMLADLAAQFPIVEATFSEASSVLGYDLWQLVQQGPAEELNKTW 60 Query: 61 NTQPALVTTSAAYLEALRQHGVN-PDYVAGHSLGEYSALVAAGALAYEEAVSLVRLRGRF 119 TQPAL+T S A + G P +AGHSLGEYSALV AG L +++AVSLV LRG+ Sbjct: 61 QTQPALLTASVAIWRIWQHQGGKLPSLMAGHSLGEYSALVCAGVLDFKQAVSLVELRGKL 120 Query: 120 MEEAVPGGQGAMAAVLGAEREALAELCRSISEAGNSVELANVNCPGQIVISGSKAGVEEA 179 M+EAVP G GAM A++G + E++A+ C S G V N N PGQ+VI+G+K VE A Sbjct: 121 MQEAVPEGTGAMYAIIGLDNESIAKACEE-SAQGQVVSPVNFNSPGQVVIAGNKDAVERA 179 Query: 180 AQRIKEIGGKRAIPLEVSGPFHSSLMKEAAERLAAELGKXXXXXXXXXXXXXXXXXXXXD 239 K G KRA+PL VS P H +LMK AA++LA L K Sbjct: 180 GAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVALEKIDFQEPLFPVVNNVDVKAEVS 239 Query: 240 PDEIRELLMQQVFSPVLWQDSVEWLIAAGVDTFVEIGPGSVLAGLIRKIDKTVKVVNINS 299 P IR L++Q+++PV W +SVE++ A GV+ +EIGPG VL GL ++I ++ +N Sbjct: 240 PVAIRSALVRQLYNPVRWTESVEFIAAEGVELLLEIGPGKVLTGLTKRIVDSLAAAAVND 299 Query: 300 L 300 + Sbjct: 300 V 300 Score = 228 bits (581), Expect = 9e-60, Method: Compositional matrix adjust. Identities = 119/299 (39%), Positives = 171/299 (57%), Gaps = 2/299 (0%) Query: 1 MGKIAFVFPGQGAQAVGMGKDVYDALPHSRAVFEKGDEVLGFPLSRLIFEGPDSELKQTV 60 M KIAF+FPGQG+Q +GMGK++Y+ +P ++ +F++ DE L LS LIFEG EL T Sbjct: 1 MSKIAFLFPGQGSQFIGMGKELYEQVPAAKRLFDEADETLETKLSSLIFEGDAEELTLTY 60 Query: 61 NTQPALVTTSAAYLEALRQHGVN-PDYVAGHSLGEYSALVAAGALAYEEAVSLVRLRGRF 119 N QPAL+TTS A LE ++ G+ + + A + ++ + Sbjct: 61 NAQPALLTTSIAVLEKFKESGITPDFTAGHSLGEYSALVAAGALSFKDAVYTVRKRGEFM 120 Query: 120 MEEAVPGGQGAMAAVLGAEREALAELCRSISEAGNSVELANVNCPGQIVISGSKAGVEEA 179 E G A + + E GN V+LAN+NCPGQIVISG+ GVE A Sbjct: 121 NEAVPAGEGAMAAILGMDAEALKQVTDKVTEE-GNLVQLANLNCPGQIVISGTAKGVELA 179 Query: 180 AQRIKEIGGKRAIPLEVSGPFHSSLMKEAAERLAAELGKXXXXXXXXXXXXXXXXXXXXD 239 ++ KE G KRAIPLEVSGPFHS LMK AAE+L L + Sbjct: 180 SELAKENGAKRAIPLEVSGPFHSELMKPAAEKLKEVLDACDIKDADVPVISNVSADVMTE 239 Query: 240 PDEIRELLMQQVFSPVLWQDSVEWLIAAGVDTFVEIGPGSVLAGLIRKIDKTVKVVNIN 298 +I+E L++Q++SPV +++S+ LIA GV TF+EIGPG VL+GL++K+++ +K + ++ Sbjct: 240 KADIKEKLIEQLYSPVRFEESINKLIAEGVTTFIEIGPGKVLSGLVKKVNRRLKTIAVS 298 >PA2968 [I] COG0331 (acyl-carrier-protein) S-malonyltransferase Length = 312 Score = 206 bits (523), Expect = 5e-53, Method: Compositional matrix adjust. Identities = 127/299 (42%), Positives = 171/299 (57%), Gaps = 2/299 (0%) Query: 4 IAFVFPGQGAQAVGMGKDVYDALPHSRAVFEKGDEVLGFPLSRLIFEGPDSELKQTVNTQ 63 +AFVFPGQG+Q++GM ++ R F + E LG+ L L+ GP+ L QT TQ Sbjct: 5 LAFVFPGQGSQSLGMLAELGAQQALVRDTFAEASEALGYDLWALVQNGPEERLNQTDKTQ 64 Query: 64 PALVTTSAAYLEA-LRQHGVNPDYVAGHSLGEYSALVAAGALAYEEAVSLVRLRGRFMEE 122 PA++T S A L + G P +VAGHSLGEYSALVAA +LA+ +AV LV RG+ M++ Sbjct: 65 PAILTVSIALWRLWLAEGGARPAFVAGHSLGEYSALVAAESLAFADAVKLVERRGQLMQQ 124 Query: 123 AVPGGQGAMAAVLGAEREALAELCRSISEAGNSVELANVNCPGQIVISGSKAGVEEAAQR 182 AVP GQG MAA+LG E + C ++ G V N N PGQ+VI+G+ A VE A + Sbjct: 125 AVPAGQGGMAAILGLEDADVLAACAEAAQ-GEVVSAVNFNAPGQVVIAGAAAAVERAIEA 183 Query: 183 IKEIGGKRAIPLEVSGPFHSSLMKEAAERLAAELGKXXXXXXXXXXXXXXXXXXXXDPDE 242 K G KRA+ L VS P H LM+ AAE+ AA + D D Sbjct: 184 CKARGAKRAVALPVSVPSHCELMRPAAEQFAASVESLQWQAPKISLVQNVSAAVPADLDT 243 Query: 243 IRELLMQQVFSPVLWQDSVEWLIAAGVDTFVEIGPGSVLAGLIRKIDKTVKVVNINSLE 301 +R L+ Q++SPV W +S++ L GV VE GPG VLAGL R+ K + ++ +E Sbjct: 244 LRRDLLAQLYSPVRWVESIQLLAEKGVTELVECGPGKVLAGLNRRCAKGINTHGLDGVE 302 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.133 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,823,564 Number of extensions: 785419 Number of successful extensions: 9401 Number of sequences better than 1.0e-05: 46 Number of HSP's gapped: 9302 Number of HSP's successfully gapped: 46 Length of query: 307 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 201 Effective length of database: 26,153,027 Effective search space: 5256758427 Effective search space used: 5256758427 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 115 (48.9 bits)