BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0557.0042.p (316 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value all3918 [P] COG2897 Rhodanese-related sulfurtransferase 266 4e-71 DR2531 [P] COG2897 Rhodanese-related sulfurtransferase 243 4e-64 PA1292 [P] COG2897 Rhodanese-related sulfurtransferase 211 8e-55 SMc01172 [P] COG2897 Rhodanese-related sulfurtransferase 146 5e-35 Cgl2717 [P] COG2897 Rhodanese-related sulfurtransferase 134 2e-31 >all3918 [P] COG2897 Rhodanese-related sulfurtransferase Length = 277 Score = 266 bits (679), Expect = 4e-71, Method: Compositional matrix adjust. Identities = 134/287 (46%), Positives = 177/287 (61%), Gaps = 14/287 (4%) Query: 20 EEGILMDA--TVTKRWLLARLYEPEQTIVDCRFTLGKPEAGRESYEQEHIPGAVYLDLEH 77 E+ ++ D V+ WLL L+ + IVDCRF+L P+ G++ Y+ HI GA YLDL Sbjct: 2 EKSLMTDTQLVVSSSWLLQNLHNIDVVIVDCRFSLADPQLGKQQYQTSHIEGAYYLDLNL 61 Query: 78 DLSSPVGEHGGRHPLPDPQVLAARLAKLGIG-NGSRIVAYDDESGMNAARLWWLLRYLGH 136 DLSSPVGEHGGRHPLP+P LA +LA +GI + +VAYDD A+RLWWLLRYLGH Sbjct: 62 DLSSPVGEHGGRHPLPNPDELAQKLASMGIEFQKTLVVAYDDSRFAFASRLWWLLRYLGH 121 Query: 137 ENVFILEGGFSAWKAEKFPVTDHQPVRVPSSFEPKVQPQMLADVNEVRRISEXXXXXXXX 196 E V +L GGFS W+ +P+TD P ++F P++Q Q+L D+ +V+ + Sbjct: 122 EQVAVLNGGFSQWEQAGYPITDVIPQLKTANFVPQIQTQLLVDIEDVKNRKD-------- 173 Query: 197 XXXXXXXXXIDSRAHERYLGLEETLDKKAGHIPGAVNFFWKNTQNTDGSVKSAEQLQEHF 256 +DSR +RY G E +DK AGHI GAVN+ W ++ G + Q+ + Sbjct: 174 ---LPTVVLVDSRESDRYRGEREPIDKIAGHIAGAVNYPWMEVTDSSGYSIPLTKHQQRW 230 Query: 257 AGLDKDAEIIVYCGSGVTACPNVLALEKAGYKNVRLYPGSWSDWISY 303 L EI+VYCGSGVTAC N+L+LE AG +LY GSWSDWISY Sbjct: 231 QNLATAEEILVYCGSGVTACVNLLSLEVAGIHTGKLYAGSWSDWISY 277 >DR2531 [P] COG2897 Rhodanese-related sulfurtransferase Length = 283 Score = 243 bits (619), Expect = 4e-64, Method: Compositional matrix adjust. Identities = 143/293 (48%), Positives = 176/293 (60%), Gaps = 28/293 (9%) Query: 30 TKRWLLARLYEPEQT-IVDCRFTLGKPEAGRESYEQEHIPGAVYLDLEHDLSSPV--GEH 86 + WLLA+ P ++DCR+ L P GR +Y + HIPGAVY DLE DLS PV G Sbjct: 9 SAAWLLAQ--RPGAVRVLDCRYALSDPLLGRLAYLEGHIPGAVYADLETDLSGPVQPGGE 66 Query: 87 GGRHPLPDPQVLAARLAKLGIGNGSRIVAYDDESGMN---AARLWWLLRYLGHENVFILE 143 GGRHPLPDP VLA L GIGNG +V YDD G AAR WWLLR+LGH V++L+ Sbjct: 67 GGRHPLPDPPVLADWLGAAGIGNGDVVVCYDDPGGGQGFYAARAWWLLRWLGHREVYVLD 126 Query: 144 GGFSAWKAEKFPVTDHQPVRVPSSFEPKVQPQMLADVNEVRRISEXXXXXXXXXXXXXXX 203 GG+ A+ A + +P VP++F P VQP+++A +V+ + Sbjct: 127 GGWPAYLAAGGEASTEEPHPVPTTFTPDVQPELVATAQDVQNRED-------------GV 173 Query: 204 XXIDSRAHERYLGLEETLDKKAGHIPGAVNFFWKNTQNTDGSVKS-AEQLQEHFAGLDKD 262 IDSRA ERY G E LDKKAGHIPGAVN + + G ++ AEQ AG D Sbjct: 174 LLIDSRAPERYRGDSEPLDKKAGHIPGAVNRPFSAALDEQGQFRAGAEQAGRLAAG---D 230 Query: 263 AEIIVYCGSGVTACPNVLALEKAGY---KNVRLYPGSWSDWISYEGNPIATGE 312 A I+YCGSGV+A PN+LA E AG N RLY GSWSDW+S EG P+ATGE Sbjct: 231 APTIIYCGSGVSATPNLLARELAGVPLGPNNRLYAGSWSDWVSDEGRPVATGE 283 >PA1292 [P] COG2897 Rhodanese-related sulfurtransferase Length = 284 Score = 211 bits (538), Expect = 8e-55, Method: Compositional matrix adjust. Identities = 117/289 (40%), Positives = 163/289 (56%), Gaps = 16/289 (5%) Query: 29 VTKRWLLARLYEPEQTIVDCRFTLGKPEAGRESYEQEHIPGAVYLDLEHDLSSPVGEH-G 87 +T + L ARL EP+ ++DCRF L P G Y++ HIPGA + DLE DLS+PV + Sbjct: 7 LTAQQLAARLSEPDLLVLDCRFALEDPSYGARVYQENHIPGAHFADLERDLSAPVRKGVT 66 Query: 88 GRHPLPDPQVLAARLAKLGIGNGSRIVAYDDESGMNAARLWWLLRYLG-HENVFILEGGF 146 GRHPLPDP LA +L G+ S++V YDD G AAR WWLL +LG + V++L+GG Sbjct: 67 GRHPLPDPAELALKLQAWGLRQDSQVVLYDDGPGAFAARAWWLLHWLGKRDGVYLLDGGL 126 Query: 147 SAWKAEKFPVTDHQPVRVPSSFEPKVQPQMLADVNEVRRISEXXXXXXXXXXXXXXXXXI 206 +AWKA +T+ + P F+ + +L D ++ + Sbjct: 127 AAWKAAGLALTNGESSLRPGDFQGQPDASLLIDAATLQ-----------AQLGQPGLALL 175 Query: 207 DSRAHERYLGLEETLDKKAGHIPGAVNFFWKNTQNTDGSVKSAEQLQEHFAGLDKDA--- 263 D+RA R+ G E +D AGHIPGA + + +DG EQL + F+ L + Sbjct: 176 DARAQPRFRGEVEPIDPVAGHIPGAQCAAFTDNLGSDGRFLPPEQLHQRFSALLRGRPVD 235 Query: 264 EIIVYCGSGVTACPNVLALEKAGYKNVRLYPGSWSDWISYEGNPIATGE 312 E++ YCGSGVTAC N+ AL AG+ RLY GSWS+WI+ P+ATG+ Sbjct: 236 ELVAYCGSGVTACHNLFALSLAGFPLPRLYAGSWSEWITDPRRPVATGD 284 >SMc01172 [P] COG2897 Rhodanese-related sulfurtransferase Length = 287 Score = 146 bits (368), Expect = 5e-35, Method: Compositional matrix adjust. Identities = 92/292 (31%), Positives = 135/292 (46%), Gaps = 24/292 (8%) Query: 29 VTKRWLLARLYEPEQTIVDCRFTLGKPEAGRES---YEQEHIPGAVYLDLEHDLSSPVGE 85 V+ WL RL +P I+D + L P R++ Y HIPGAV+ D + + Sbjct: 13 VSADWLEQRLDDPSVKIIDAAWYL--PAQNRDAKAEYAAAHIPGAVFFDQDAIANR---T 67 Query: 86 HGGRHPLPDPQVLAARLAKLGIGNGSRIVAYDDESGMNAARLWWLLRYLGHENVFILEGG 145 H LP P+ + + +GI IV YD A R+WW+ R +G ENVF+L+GG Sbjct: 68 SALPHTLPSPEAFSEAVGAMGISENDTIVVYDGPGIFTAPRVWWMFRIMGAENVFVLDGG 127 Query: 146 FSAWKAEKFPVTDHQPVRVPSSFEPKVQPQMLADVNEVRRISEXXXXXXXXXXXXXXXXX 205 WKA+ P T P +F P + +R + E Sbjct: 128 MDGWKADGRPTTTEVAKPSPQAFNAVFNPNAVTSFERMRDVVEHRLSQIA---------- 177 Query: 206 IDSRAHERYLG--LEETLDKKAGHIPGAVNFFWKNTQNTDGSVKSAEQLQEHF--AGLDK 261 D+R+ R+ G E ++GH+PGA + + G K + L+ F AG+D Sbjct: 178 -DARSAGRFAGEEPEPRAGMRSGHMPGARSLP-SGVFSDKGRFKDLDALRRTFADAGIDL 235 Query: 262 DAEIIVYCGSGVTACPNVLALEKAGYKNVRLYPGSWSDWISYEGNPIATGEE 313 ++ CGSG+TA LAL+ G+K+ LY GSWS+W S P+A G+E Sbjct: 236 TKPVVTSCGSGITAAIITLALQSLGHKDNTLYDGSWSEWGSRPDTPVAIGKE 287 >Cgl2717 [P] COG2897 Rhodanese-related sulfurtransferase Length = 274 Score = 134 bits (337), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 94/294 (31%), Positives = 132/294 (44%), Gaps = 32/294 (10%) Query: 25 MDATVTKRWLLARLYEPEQTIVDCRFTLGKPEAGRESYEQEHIPGAVYLDLEHDLSSPVG 84 M ++ L ++ ++ V F AGR + EHIP +++ D +LS Sbjct: 1 MTVLISPSTLAESIHAGKKQTVLAAFWAPIEGAGRTVFCSEHIPTSIFCDPALELSGVPS 60 Query: 85 EHGGRHPLPDPQVLAARLAKLGIGNGSRIVAYDDESGMNAARLWWLLRYLGHENVFILEG 144 GR+PLP VLA G+ IV YD G+ AAR WW+LR+ G NV IL+G Sbjct: 61 SEDGRNPLPPLNVLARSFRTWGLNTDREIVFYDQGRGLFAARAWWILRWAGMPNVRILDG 120 Query: 145 GFSAWKAEKFPVTDHQ------PVRVPSSFEPKVQPQML--ADVNEVRRISEXXXXXXXX 196 GF W+ DH+ P P + P L A + +V+ Sbjct: 121 GFQKWE-------DHELGHAGGPGNFPHFCNVRPNPGQLSVATIEDVK------------ 161 Query: 197 XXXXXXXXXIDSRAHERYLGLEETLDKKAGHIPGAVNFFWKNTQNTDGSVKSAEQLQEHF 256 IDSR +R+ G E LD KAGHIPGA+N K+ D + KS E++++ F Sbjct: 162 ---AHQGILIDSRDEQRFAGRSEKLDLKAGHIPGAININAKSLLEDDFTFKSPEEIRQIF 218 Query: 257 A--GLDKDAEIIVYCGSGVTACPNVLALEKAGYKNVRLYPGSWSDWISYEGNPI 308 A G+ +IVY GSG + + +E AG Y WS W + NPI Sbjct: 219 ADKGVTSGENVIVYSGSGNHSSQLLAGMEHAGLTGASHYFAGWSQWSANPENPI 272 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,671,293 Number of extensions: 1027109 Number of successful extensions: 1996 Number of sequences better than 1.0e-05: 38 Number of HSP's gapped: 1874 Number of HSP's successfully gapped: 43 Length of query: 316 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 210 Effective length of database: 26,153,027 Effective search space: 5492135670 Effective search space used: 5492135670 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)