BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0557.0031.p (210 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mlr0915 [Q] COG3315 O-Methyltransferase involved in polyketide b... 79 6e-15 MA2763 [Q] COG3315 O-Methyltransferase involved in polyketide bi... 75 9e-14 MA2468 [Q] COG3315 O-Methyltransferase involved in polyketide bi... 73 3e-13 MA2745 [Q] COG3315 O-Methyltransferase involved in polyketide bi... 71 1e-12 MA2903 [Q] COG3315 O-Methyltransferase involved in polyketide bi... 71 1e-12 >mlr0915 [Q] COG3315 O-Methyltransferase involved in polyketide biosynthesis Length = 301 Score = 78.6 bits (192), Expect = 6e-15, Method: Compositional matrix adjust. Identities = 5/208 (2%), Positives = 27/208 (12%), Gaps = 20/208 (9%) Query: 1 MHGARQYLIFAAGYDTFAYRQPEWAAKLQIFEIDHPATADDKRRRVSALNPTKPSNLHYI 60 + + + A + Sbjct: 98 GTRWTPDDEMVLNKLREGKAPVI--LAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPIS 155 Query: 61 SFDFNEQDLQKRLLDCRAFDPGVISFCSLLGITYYLSKDVFKHFLATITKLLPGGSSIVF 120 + D ++ F + + + L G+ + + Sbjct: 156 AETGLNVDTIAAIVRK-HLPEATHHFPEDYITDRSQRFMASEIIREKLMRFL--GAELPY 212 Query: 121 DYPDEHTFTPSAKERTQKQVMMA------ARAGEPMQACYSYEELERMLEDVNLLIYQHF 174 + + ++ + + + + Sbjct: 213 SVTV-----EIERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQE 267 Query: 175 TPDQITGQLFKA----YNDSQPAHPIRA 198 + Sbjct: 268 MFEAPVHLELWVKVKSGWADDERALRSL 295 >MA2763 [Q] COG3315 O-Methyltransferase involved in polyketide biosynthesis Length = 299 Score = 74.7 bits (182), Expect = 9e-14, Method: Compositional matrix adjust. Identities = 48/198 (24%), Positives = 91/198 (45%), Gaps = 2/198 (1%) Query: 3 GARQYLIFAAGYDTFAYRQPEWAAKLQIFEIDHPATADDKRRRVSALNPTKPSNLHYISF 62 G Q ++ AGYDT AYR ++++FE+DHP T K + + + P+++ Y+ Sbjct: 97 GLEQLVVLGAGYDTRAYRIEGLKGEIKVFEVDHPDTQYVKIKNIKEIFGFLPNHIIYVPV 156 Query: 63 DFNEQDLQKRLLDCRAFDPGVISFCSLLGITYYLSKDVFKHFLATITKLLPGGSSIVFDY 122 DF ++ +RL++ + +D + + + G+ Y+ + L+ I + GS+I+FDY Sbjct: 157 DFETENFGERLME-KGYDRSLKTLFIMEGLVVYIPPEAVDEALSFIAENSGKGSTILFDY 215 Query: 123 PDEHTFTPSAKERTQKQVM-MAARAGEPMQACYSYEELERMLEDVNLLIYQHFTPDQITG 181 E S + K + AA+ GEP + +E L + + T + Sbjct: 216 FPESVVDGSCELEVGKNMRNYAAKQGEPFKFGIKESAVETFLSERGFSQIHNVTAEDYKR 275 Query: 182 QLFKAYNDSQPAHPIRAF 199 F N ++ + +F Sbjct: 276 IYFSGVNKNREVCSLLSF 293 >MA2468 [Q] COG3315 O-Methyltransferase involved in polyketide biosynthesis Length = 350 Score = 73.2 bits (178), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 43/121 (35%), Positives = 64/121 (52%), Gaps = 2/121 (1%) Query: 2 HGARQYLIFAAGYDTFAYRQPEWAAKLQIFEIDHPATADDKRRRVSALNPTKPSNLHYIS 61 G Q +I AG+DT AYR K+++FE+DHP T + K +R+ + P ++ ++ Sbjct: 139 EGLDQVVILGAGFDTRAYRIAG-IEKMRVFEVDHPETQEVKLKRLKKVINPMPDHVTFVQ 197 Query: 62 FDFNEQDLQKRLLDCRAFDPGVISFCSLLGITYYLSKDVFKHFLATITKLLPGGSSIVFD 121 DFN Q L KRLL +D + G+T YL+ + LA I GSS++FD Sbjct: 198 VDFNTQMLGKRLL-ASGYDEQGKTLFIWQGVTMYLTPEGIDSTLAFIANHSGTGSSVIFD 256 Query: 122 Y 122 Y Sbjct: 257 Y 257 >MA2745 [Q] COG3315 O-Methyltransferase involved in polyketide biosynthesis Length = 305 Score = 71.2 bits (173), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 52/198 (26%), Positives = 84/198 (42%), Gaps = 3/198 (1%) Query: 3 GARQYLIFAAGYDTFAYRQPEWAAKLQIFEIDHPATADDKRRRVSALNPTKPSNLHYISF 62 G Q ++ AGYD+ AYR E ++++FE+D P T K +V + P ++ Y+ Sbjct: 104 GLEQLVVLGAGYDSRAYR-IEGLERVRVFEVDVPTTQKLKTDKVREIFGVLPGHVTYVPA 162 Query: 63 DFNEQDLQKRLLDCRAFDPGVISFCSLLGITYYLSKDVFKHFLATITKLLPGGSSIVFDY 122 D DL +RL + +D + L G+ YYL LA I GS++VFDY Sbjct: 163 DLAVDDLGQRLAES-GYDTSRKTLFLLEGLVYYLPPTAVDKVLAFIAHSSGRGSAMVFDY 221 Query: 123 PDEHTFTPSAKERTQKQVMMAAR-AGEPMQACYSYEELERMLEDVNLLIYQHFTPDQITG 181 + ++ + A AGEP LE L + + Q T Sbjct: 222 IPASLVDGTCEQEAGRNWQKAVTDAGEPFLFGVDEVALEAFLSERGFTLRQKMTGGDYRT 281 Query: 182 QLFKAYNDSQPAHPIRAF 199 + N+ +P + + +F Sbjct: 282 AYLRGKNEGKPVNSLLSF 299 >MA2903 [Q] COG3315 O-Methyltransferase involved in polyketide biosynthesis Length = 211 Score = 70.9 bits (172), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 0/202 (0%), Positives = 0/202 (0%), Gaps = 10/202 (4%) Query: 3 GARQYLIFAAGYDTFAYRQPEWAAKLQIFEIDHPATADDKRRRVSALNPTKPSNLHYISF 62 + + ++ K V + + Sbjct: 82 YNSKAENIIKTCRILLEQ-HNGEVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTH 140 Query: 63 DFNEQDLQKRLLDCRAFDPGVISFCSLLGITYYLSKDVFKHFLATITKLLPGGSSIVFDY 122 F + + + ++ + + + T + Sbjct: 141 IFRVCNRTQFAPGK-NVEQVEEKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCII 199 Query: 123 -----PDEHTFTPSAKERTQKQVMMAARAGEPMQACYSYEELERMLEDVNLLIYQHFTPD 177 Sbjct: 200 EDLCE--- 256 Query: 178 QITGQLFKAYNDSQPAHPIRAF 199 Sbjct: 257 278 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.137 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,559,940 Number of extensions: 601390 Number of successful extensions: 1388 Number of sequences better than 1.0e-05: 7 Number of HSP's gapped: 1389 Number of HSP's successfully gapped: 7 Length of query: 210 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 108 Effective length of database: 26,614,363 Effective search space: 2874351204 Effective search space used: 2874351204 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 113 (48.1 bits)