BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0557.0020.p (290 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin1782 [R] COG0596 Predicted hydrolases or acyltransferases (al... 93 4e-19 PM0355 [R] COG0596 Predicted hydrolases or acyltransferases (alp... 85 1e-16 VC2097 [R] COG0596 Predicted hydrolases or acyltransferases (alp... 82 8e-16 HI0193 [R] COG0596 Predicted hydrolases or acyltransferases (alp... 77 3e-14 AF1706 [R] COG0596 Predicted hydrolases or acyltransferases (alp... 74 2e-13 >lin1782 [R] COG0596 Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) Length = 301 Score = 93.2 bits (230), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 14/264 (5%), Positives = 45/264 (17%), Gaps = 15/264 (5%) Query: 32 DTKTEGPAVLCLHGRWGRAETWVDFIHQYGKQFRVIAPDQRGHGLSSKP--VSNYTAEEM 89 L + + + + R++ G + + E+ Sbjct: 13 IVGRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIYVDTPGLHMEEKR 72 Query: 90 AEDIKELIIHLNLESVIIVGHSMGGGVAGYMAALHPEYIQALAILDKSANGPDERNPVSL 149 A + + + + + G N Sbjct: 73 AINRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREGKAPVILAVNKVDNVQE- 131 Query: 150 EQIPA----VDPVTKDWPLPFSSLCE--AKHYIRGAMVSELSYQYFINSLIETPEGYEMM 203 + ++ L + + A + + E Sbjct: 132 -KADLLPHLQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHLPEATHHFPEDYITDRSQ 190 Query: 204 FSPQAIAANIAYYVNWYSLLPSIKCPVLLIRAKNSEGLPDADYMKMQSLLPNCHAYEISH 263 + L + + + + + + L+ + Sbjct: 191 RFMASEIIREKLMRF----LGAELPYSVTVEIERFVSN-ERGGYDINGLILVEREGQKKM 245 Query: 264 PDHNVHLGNKEEFYTCMDLFLDGL 287 N K + Sbjct: 246 VIGNKGAKIKTIGIEARKDMQEMF 269 >PM0355 [R] COG0596 Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) Length = 262 Score = 84.7 bits (208), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 70/267 (26%), Positives = 112/267 (41%), Gaps = 19/267 (7%) Query: 23 LTHIDMFYMDTKTEGPAVLCLHGRWGRAETWVDFIHQYGKQFRVIAPDQRGHGLS-SKPV 81 L H + + P + +HG +G + +++ ++ D R HG S Sbjct: 6 LLHFQFHQLKQEINAPTFVFIHGLFGDMNNLGIIARAFSEKYPILRVDLRNHGQSFHHEE 65 Query: 82 SNYTAEEMAEDIKELIIHLNLESVIIVGHSMGGGVAGYMAALHPEYIQALAILD----KS 137 NYT MAED+ +I L LE VI++GHSMGG A M AL+P ++ L ++D K Sbjct: 66 MNYTL--MAEDLANVIHTLQLEKVILIGHSMGGKTAMKMTALYPHLVEKLIVIDIAPVKY 123 Query: 138 ANGPDERNPVSLEQIPAVDPVTKDWPLPFSSLCEAKHYIRGAMVSELSYQYFINSLIETP 197 + + L P T+ EAKHY+ + E Q+ + S Sbjct: 124 GHHGHDAVFAGLFATKQAKPKTRQ---------EAKHYLAQYIPEEAIQQFMLKSFDANA 174 Query: 198 EGYEMMFSPQAIAANIAYYVNWYSLLPSIKCPVLLIRAKNSEGLPDADYMKMQSLLPNCH 257 + Y F+ A+ AN + ++W ++ P L IR S + D + + P Sbjct: 175 KEY-FRFNLSALHANYPHIMDWQPCHCTV--PTLFIRGGQSNYIKTEDTQPILAQFPQAT 231 Query: 258 AYEISHPDHNVHLGNKEEFYTCMDLFL 284 A+ I+ H VH E ++ FL Sbjct: 232 AFTINGCGHWVHAEKPEFVIRAIERFL 258 >VC2097 [R] COG0596 Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) Length = 257 Score = 82.4 bits (202), Expect = 8e-16, Method: Compositional matrix adjust. Identities = 64/254 (25%), Positives = 111/254 (43%), Gaps = 17/254 (6%) Query: 37 GPAVLCLHGRWGRAETWVDFIHQYGKQFRVIAPDQRGHGLS-SKPVSNYTAEEMAEDIKE 95 G V+ +HG +G + +V++ D R HGLS NY MA+D+ + Sbjct: 13 GETVVLIHGLFGSLDNLGLLARDLKNDHQVLSLDLRNHGLSFHSDEHNYAL--MAQDVNQ 70 Query: 96 LIIHLNLESVIIVGHSMGGGVAGYMAALHPEYIQALAILDKSANGPDERNP----VSLEQ 151 L+ HLNL SV+++GHSMGG VA +A + E ++ L +LD S +R LE Sbjct: 71 LLEHLNLTSVVVIGHSMGGKVAMKLADIAAEKVRQLVVLDMSPVAYSQRRHDNVFAGLEA 130 Query: 152 IPAVDPVTKDWPLPFSSLCEAKHYIRGAMVSELSYQYFINSLIETPEGYEMMFSPQAIAA 211 + P ++ + + + +R Q+ SL+ F+ A+ A Sbjct: 131 VLVQKPTSRSEVMAILAQHIEQEGVR---------QFLGKSLMSEQNVMTWRFNVAALKA 181 Query: 212 NIAYYVNWYSLLPSIKCPVLLIRAKNSEGLPDADYMKMQSLLPNCHAYEISHPDHNVHLG 271 + A + W ++ + P L I+ +S+ L +Q+ A+ I++ H +H Sbjct: 182 HYAEILGW-DIIAKCRIPTLFIKGADSDYLTTQHQPMVQAQFSQAKAHVIANTGHWLHAE 240 Query: 272 NKEEFYTCMDLFLD 285 E + F+D Sbjct: 241 KPAEVIRAIRKFID 254 >HI0193 [R] COG0596 Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) Length = 287 Score = 77.0 bits (188), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 62/237 (26%), Positives = 109/237 (45%), Gaps = 19/237 (8%) Query: 38 PAVLCLHGRWGRAETWVDFIHQYGKQFRVIAPDQRGHGLSSKPVS-NYTAEEMAEDIKEL 96 P ++ +HG +G + + + + ++ D R HG S NY + MAED+ + Sbjct: 47 PVLIFIHGLFGDMDNLGVIARAFSEHYSILRIDLRNHGHSFHSEKMNY--QLMAEDVIAV 104 Query: 97 IIHLNLESVIIVGHSMGGGVAGYMAALHPEYIQALAILDKSANGPDERNPVSLEQIPAVD 156 I HLNL VI++GHSMGG A + AL PE ++ L ++D S P+ E D Sbjct: 105 IRHLNLSKVILIGHSMGGKTAMKITALCPELVEKLIVIDMS--------PMPYEGFGHKD 156 Query: 157 PVTKDWPLPFS---SLCEAKHYIRGAMVSELSYQYFINSL-IETPEGYEMMFSPQAIAAN 212 + + + + +AK ++ + E Q+ + S + + + + F+ A+ N Sbjct: 157 VFNGLFAVKNAKPENRQQAKPILKQEINDEDVVQFMLKSFDVNSADCFR--FNLTALFNN 214 Query: 213 IAYYVNWYSLLPSIKCPVLLIRAKNSEGLPDADYMKMQSLLPNCHAYEISHPDHNVH 269 A ++W + + P L I+ NS + + K+ PN A+ I+ H VH Sbjct: 215 YANIMDWEKV--RVFTPTLFIKGGNSSYIKIENSEKILEQFPNATAFTINGSGHWVH 269 >AF1706 [R] COG0596 Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) Length = 238 Score = 74.3 bits (181), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 63/223 (28%), Positives = 100/223 (44%), Gaps = 16/223 (7%) Query: 36 EGPAVLCLHGRWGRAETWVDFIHQYGKQFRVIAPDQRGHGLSSKPVSNYTAEEMAEDIKE 95 EG ++ +HG E W + K FRV+A D RG G+S P S + E+ A D+K Sbjct: 26 EGEPLILIHGLGDCIEGWTFQYDDFSKHFRVVALDLRGFGMSDVPES-ISVEDFARDVKN 84 Query: 96 LIIHLNLESVIIVGHSMGGGVAGYMAALHPEYIQALAILDKSANGPDERNPVSLEQIPAV 155 LI HL +E ++G SMGG V +PE +++L + + PD + EQ + Sbjct: 85 LIDHLGIERANLLGLSMGGVVCMEFYRQYPEMVKSLVLANTLHKLPDAGRAM-FEQRLKL 143 Query: 156 DPVTKDWPLPFSSLCEAKHYIRGAMVSELSYQYFINSLIE--TPEGYEMMFSPQAIAANI 213 + D + E + + EL + ++I E Y + A + Sbjct: 144 LESSPDMTQIAEFIAEMSIHQDRPELKEL-----VKTIIRKNNKEFY------TKVTAEL 192 Query: 214 AYYVNWYSLLPSIKCPVLLIRAKNSEGLPDADYMKMQSLLPNC 256 +N+ LLP I+ P L+I A+ P A ++ SL+P C Sbjct: 193 G-RINYEDLLPKIEVPTLVITAEFDVTTPPALGREIASLIPKC 234 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.136 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,110,369 Number of extensions: 909463 Number of successful extensions: 1881 Number of sequences better than 1.0e-05: 65 Number of HSP's gapped: 1892 Number of HSP's successfully gapped: 65 Length of query: 290 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 185 Effective length of database: 26,268,361 Effective search space: 4859646785 Effective search space used: 4859646785 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)