BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0554.0008.p (323 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0777 [C] COG0022 Pyruvate/2-oxoglutarate dehydrogenase complex... 335 4e-92 SSO1526 [C] COG0022 Pyruvate/2-oxoglutarate dehydrogenase comple... 324 9e-89 mll3628 [C] COG0022 Pyruvate/2-oxoglutarate dehydrogenase comple... 307 1e-83 BH1823 [C] COG0022 Pyruvate/2-oxoglutarate dehydrogenase complex... 301 9e-82 BS_acoB [C] COG0022 Pyruvate/2-oxoglutarate dehydrogenase comple... 294 1e-79 >BH0777 [C] COG0022 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit Length = 553 Score = 335 bits (860), Expect = 4e-92, Method: Compositional matrix adjust. Identities = 23/324 (7%), Positives = 61/324 (18%), Gaps = 1/324 (0%) Query: 1 MRKMTYGEGIREGMRSKMLEDPNVVLLGEDVGPYGGTFGVTKGLWEEFGEERVRDTPISE 60 M + + + + + + T Sbjct: 1 MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLM 60 Query: 61 XXXXXXXXXXXXXXLRPVAELMFIDFLTFGMDGLVNQAAKMRYMFGGKISVPLVLRLPAG 120 + + + A + K PL + Sbjct: 61 LQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATS 120 Query: 121 GGISAGAQHSGSLEAWVTHVPGLKVVYPSNAQDAWGMMLTAIEDDNPVVYIESKVLYSKK 180 I ++ + + AQ GM E K+ Sbjct: 121 RNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIV 180 Query: 181 FEVDDTVAAVPFGVASVKREGSDVSIITYGKQVDDALNAAKVLAEEGIEAEVIDLRSLYP 240 + D + +A + + ++ + L + E +L + Sbjct: 181 TDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLT 240 Query: 241 LDKEAIFSSVAKTHRALVVTEEVKRGGYGGELSAMISEL-CFDELDAPVVRIGALDTPVP 299 +D + +A++ + + A+ + + + Sbjct: 241 IDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASAL 300 Query: 300 YSPALEAYVLPNADDIIQGIKSMF 323 A L Sbjct: 301 EETAASMEQLTATVKQNADNARQA 324 >SSO1526 [C] COG0022 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit Length = 553 Score = 324 bits (831), Expect = 9e-89, Method: Compositional matrix adjust. Identities = 22/323 (6%), Positives = 61/323 (18%) Query: 1 MRKMTYGEGIREGMRSKMLEDPNVVLLGEDVGPYGGTFGVTKGLWEEFGEERVRDTPISE 60 M + + + + + + T Sbjct: 1 MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLM 60 Query: 61 XXXXXXXXXXXXXXLRPVAELMFIDFLTFGMDGLVNQAAKMRYMFGGKISVPLVLRLPAG 120 + + + A + K PL + Sbjct: 61 LQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATS 120 Query: 121 GGISAGAQHSGSLEAWVTHVPGLKVVYPSNAQDAWGMMLTAIEDDNPVVYIESKVLYSKK 180 I ++ + + AQ GM E K+ Sbjct: 121 RNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIV 180 Query: 181 FEVDDTVAAVPFGVASVKREGSDVSIITYGKQVDDALNAAKVLAEEGIEAEVIDLRSLYP 240 + D + +A + + ++ + L + E +L + Sbjct: 181 TDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLT 240 Query: 241 LDKEAIFSSVAKTHRALVVTEEVKRGGYGGELSAMISELCFDELDAPVVRIGALDTPVPY 300 +D + +A++ + + A+ + + Sbjct: 241 IDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASAL 300 Query: 301 SPALEAYVLPNADDIIQGIKSMF 323 + A + Sbjct: 301 EETAASMEQLTATVKQNADNARQ 323 >mll3628 [C] COG0022 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit Length = 553 Score = 307 bits (787), Expect = 1e-83, Method: Compositional matrix adjust. Identities = 18/323 (5%), Positives = 54/323 (16%), Gaps = 1/323 (0%) Query: 1 MRKMTYGEGIREGMRSKMLEDPNVVLLGEDVGPYGGTFGVTKGLWEEFGEERVRDTPISE 60 +R +T + L ++ + + Sbjct: 5 IRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLMLQTR 64 Query: 61 XXXXXXXXXXXXXXLRPVAELMFIDFLTFGMDGLVNQAAKMRYMFGGKISVPLVLRLPAG 120 + + ++ + + Sbjct: 65 INLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATSRNID 124 Query: 121 GGISAGAQHSGSLEAWVTHVPGLKVVYPSNAQDAWGMMLTAIEDDNPVVYIESKVLYSKK 180 L ++ + M + + ++ Sbjct: 125 EKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIVTDNA 184 Query: 181 FEVDDTVAAVPFGVASVKREGSDVSIITYGKQVDDALNAAKVLAEEGIEAEVIDLRSLYP 240 + + V + + E L Sbjct: 185 DDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLTIDGR 244 Query: 241 LDKEAIFSSVAKTHRALVVTEEVKRGGYGGELSAMISEL-CFDELDAPVVRIGALDTPVP 299 + + SV+ R+L T R G + +L + + + Sbjct: 245 SEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASALEETA 304 Query: 300 YSPALEAYVLPNADDIIQGIKSM 322 S + D + + Sbjct: 305 ASMEQLTATVKQNADNARQASQL 327 >BH1823 [C] COG0022 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit Length = 553 Score = 301 bits (771), Expect = 9e-82, Method: Compositional matrix adjust. Identities = 17/334 (5%), Positives = 51/334 (15%), Gaps = 12/334 (3%) Query: 2 RKMTYGEGIREGMRSKMLEDPNVVLLGEDVG------------PYGGTFGVTKGLWEEFG 49 ++ + + L L +T Sbjct: 3 NRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLMLQ 62 Query: 50 EERVRDTPISEXXXXXXXXXXXXXXLRPVAELMFIDFLTFGMDGLVNQAAKMRYMFGGKI 109 + + + A + + Sbjct: 63 TRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATSRN 122 Query: 110 SVPLVLRLPAGGGISAGAQHSGSLEAWVTHVPGLKVVYPSNAQDAWGMMLTAIEDDNPVV 169 G+ A+ A + + + D Sbjct: 123 IDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIVTD 182 Query: 170 YIESKVLYSKKFEVDDTVAAVPFGVASVKREGSDVSIITYGKQVDDALNAAKVLAEEGIE 229 + + V V + VA ++ + + + I+ Sbjct: 183 NADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLTID 242 Query: 230 AEVIDLRSLYPLDKEAIFSSVAKTHRALVVTEEVKRGGYGGELSAMISELCFDELDAPVV 289 + + TH + +S + A Sbjct: 243 GRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASALEE 302 Query: 290 RIGALDTPVPYSPALEAYVLPNADDIIQGIKSMF 323 +++ + + Sbjct: 303 TAASMEQLTATVKQNADNARQASQLAQSASDTAQ 336 >BS_acoB [C] COG0022 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit Length = 553 Score = 294 bits (753), Expect = 1e-79, Method: Compositional matrix adjust. Identities = 17/327 (5%), Positives = 50/327 (15%), Gaps = 12/327 (3%) Query: 2 RKMTYGEGIREGMRSKMLEDPNVVLLGEDVG------------PYGGTFGVTKGLWEEFG 49 ++ + + L L +T Sbjct: 3 NRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLMLQ 62 Query: 50 EERVRDTPISEXXXXXXXXXXXXXXLRPVAELMFIDFLTFGMDGLVNQAAKMRYMFGGKI 109 + + + A + + Sbjct: 63 TRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATSRN 122 Query: 110 SVPLVLRLPAGGGISAGAQHSGSLEAWVTHVPGLKVVYPSNAQDAWGMMLTAIEDDNPVV 169 G+ A+ A + + + D Sbjct: 123 IDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIVTD 182 Query: 170 YIESKVLYSKKFEVDDTVAAVPFGVASVKREGSDVSIITYGKQVDDALNAAKVLAEEGIE 229 + + V V + VA ++ + + + I+ Sbjct: 183 NADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLTID 242 Query: 230 AEVIDLRSLYPLDKEAIFSSVAKTHRALVVTEEVKRGGYGGELSAMISELCFDELDAPVV 289 + + TH + +S + A Sbjct: 243 GRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASALEE 302 Query: 290 RIGALDTPVPYSPALEAYVLPNADDII 316 +++ + Sbjct: 303 TAASMEQLTATVKQNADNARQASQLAQ 329 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.137 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,406,127 Number of extensions: 983405 Number of successful extensions: 2210 Number of sequences better than 1.0e-05: 35 Number of HSP's gapped: 2152 Number of HSP's successfully gapped: 35 Length of query: 323 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 216 Effective length of database: 26,037,693 Effective search space: 5624141688 Effective search space used: 5624141688 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 116 (49.3 bits)