BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0554.0007.p (338 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SSO1525 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase comple... 348 1e-95 BS_acoA [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase comple... 329 3e-90 BH1822 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex... 324 1e-88 BH0776 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex... 311 1e-84 mll3629 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase comple... 306 3e-83 >SSO1525 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit Length = 553 Score = 348 bits (892), Expect = 1e-95, Method: Compositional matrix adjust. Identities = 17/325 (5%), Positives = 59/325 (18%), Gaps = 7/325 (2%) Query: 13 ISKELLSGMYRKMLSIRKFEKTASTFFAEGKIPGFVHLYIGEEAIAVGACANLRDDDYIT 72 I L M + ++ + + F + + + Sbjct: 5 IRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLMLQTR 64 Query: 73 STHRGHGHIVAKGGNLKYMMAELFGKETGYCKGKGGSMHIADAELGILGANGIVGAGHLI 132 + + + A++ ++ + H + + + ++ Sbjct: 65 INLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATSRNID 124 Query: 133 ATGAAWSASYRGSDQVSVCFFGDASTNQSTFHEAMNLASLWKLPVIFVCENNLYGISVS- 191 + + A Q T + + + + ++ + Sbjct: 125 EKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIVTDNA 184 Query: 192 ------QARHQTIKDIAKRGEGYCMPSFTADGNDVITVYEHVQEAVRRARNGEGPTLLEF 245 Q + I + + + T+ Sbjct: 185 DDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLTIDGR 244 Query: 246 KTYRQHGHFEGDPGAYRPKEEVEAWLQKDPIPRFEKYLTENKLMTADELKQIGDEVDGEI 305 D I + + + +Q ++ Sbjct: 245 SEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASALEETA 304 Query: 306 QEALDFAFASGYPALEASVQGIYSD 330 A A + Sbjct: 305 ASMEQLTATVKQNADNARQASQLAQ 329 >BS_acoA [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit Length = 553 Score = 329 bits (844), Expect = 3e-90, Method: Compositional matrix adjust. Identities = 22/324 (6%), Positives = 56/324 (17%), Gaps = 5/324 (1%) Query: 6 RGGIQLRISKELLSGMYRKMLSIRKFEKTASTFFAEGKIPGFVHLYIGEEAIAVGACANL 65 R L + + +++S F + + + GE Sbjct: 6 RVVTLLVMVLGVF--ALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLMLQT 63 Query: 66 RDDDYITSTHRGHGHIVAKGGNLKYMMAELFGKETGYCKGKGGSMHIADAELGILGANGI 125 R + ++ + ++ +A + + I Sbjct: 64 RINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATSRNI 123 Query: 126 VGAGHLIATGAAWSASYRGSDQVSVCFFGDASTNQSTFHEAMNLASLWKLPVIFVCENNL 185 T Y F Q+ EA +L + + Sbjct: 124 DEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIVTDN 183 Query: 186 YGISVSQARHQTIKDIAKRGEGYCMPSFTADGNDVITVYEHVQEAVRRARNGEGPTLLEF 245 + + N ++ Sbjct: 184 ADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLTIDG 243 Query: 246 KTYRQHGHFEGDPGAYRPKEE-VEAWLQKDPIPRFEKYLTENKLMTADELKQIGDEVDGE 304 ++ + D I + + T + E Sbjct: 244 RSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIA--AGNTDLSSRTEQQASALE 301 Query: 305 IQEALDFAFASGYPALEASVQGIY 328 A + + + Sbjct: 302 ETAASMEQLTATVKQNADNARQAS 325 >BH1822 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit Length = 553 Score = 324 bits (831), Expect = 1e-88, Method: Compositional matrix adjust. Identities = 21/326 (6%), Positives = 55/326 (16%), Gaps = 1/326 (0%) Query: 13 ISKELLSGMYRKMLSIRKFEKTASTFFAEGKIPGFVHLYIGEEAIAVGACANLRDDDYIT 72 + L +++S F + + + GE R + + Sbjct: 11 LVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLMLQTRINLSRS 70 Query: 73 STHRGHGHIVAKGGNLKYMMAELFGKETGYCKGKGGSMHIADAELGILGANGIVGAGHLI 132 + + ++ +A + + I Sbjct: 71 AVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATSRNIDEKYKNY 130 Query: 133 ATGAAWSASYRGSDQVSVCFFGDASTNQSTFHEAMNLASLWKLPVIFVCENNLYGISVSQ 192 T Y F Q+ EA +L + + Sbjct: 131 YTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIVTDNADDYRFA 190 Query: 193 ARHQTIKDIAKRGEGYCMPSFTADGNDVITVYEHVQEAVRRARNGEGPTLLEFKTYRQHG 252 + + N ++ ++ Sbjct: 191 QWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLTIDGRSEMGDL 250 Query: 253 HFEGDPGAYRP-KEEVEAWLQKDPIPRFEKYLTENKLMTADELKQIGDEVDGEIQEALDF 311 D I + + + +Q ++ Sbjct: 251 AQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASALEETAASMEQL 310 Query: 312 AFASGYPALEASVQGIYSDIVEEARS 337 A A + + Sbjct: 311 TATVKQNADNARQASQLAQSASDTAQ 336 >BH0776 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit Length = 553 Score = 311 bits (797), Expect = 1e-84, Method: Compositional matrix adjust. Identities = 19/318 (5%), Positives = 57/318 (17%) Query: 12 RISKELLSGMYRKMLSIRKFEKTASTFFAEGKIPGFVHLYIGEEAIAVGACANLRDDDYI 71 + +L L ++ + + E + + Sbjct: 9 TLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLMLQTRINLS 68 Query: 72 TSTHRGHGHIVAKGGNLKYMMAELFGKETGYCKGKGGSMHIADAELGILGANGIVGAGHL 131 S R + N K + + K ++ + + + Sbjct: 69 RSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATSRNIDEKYK 128 Query: 132 IATGAAWSASYRGSDQVSVCFFGDASTNQSTFHEAMNLASLWKLPVIFVCENNLYGISVS 191 A + +F + ++ Sbjct: 129 NYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIVTDNADDYR 188 Query: 192 QARHQTIKDIAKRGEGYCMPSFTADGNDVITVYEHVQEAVRRARNGEGPTLLEFKTYRQH 251 A+ Q + + + + + + A TL Sbjct: 189 FAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLTIDGRSEMG 248 Query: 252 GHFEGDPGAYRPKEEVEAWLQKDPIPRFEKYLTENKLMTADELKQIGDEVDGEIQEALDF 311 + R + +++ + T + E A Sbjct: 249 DLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASALEETAASME 308 Query: 312 AFASGYPALEASVQGIYS 329 + + + Sbjct: 309 QLTATVKQNADNARQASQ 326 >mll3629 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit Length = 553 Score = 306 bits (785), Expect = 3e-83, Method: Compositional matrix adjust. Identities = 18/318 (5%), Positives = 56/318 (17%), Gaps = 1/318 (0%) Query: 14 SKELLSGMYRKMLSIRKFEKTASTFFAEGKIPGFVHLYIGEEAIAVGACANLRDDDYITS 73 S + F + +G++ L + + + D Sbjct: 23 LISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQ 82 Query: 74 THRGHGHIVAKGGNLKYMMAELFGKETGYCKGKGGSMHIADAELGILGANGIVGAGHLIA 133 ++ + + L ++ + + E + + Sbjct: 83 SNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLD 142 Query: 134 TGAAWSASYRGSDQVSVCFFGDASTNQSTFHEAMNLASLWKLPVIFVCENNLYGISVSQA 193 G + + + + + + + + L I++ Sbjct: 143 YGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVV 202 Query: 194 RHQTIKDIAKRGEGYCMPSFTADGNDVITVYEHVQEAVRRARNGEGPTLLEFKTYRQHGH 253 + R + I R+ G ++ Sbjct: 203 LILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLT 262 Query: 254 FEGDPGAYRPKEEVEAWL-QKDPIPRFEKYLTENKLMTADELKQIGDEVDGEIQEALDFA 312 + + + Q A + Sbjct: 263 DTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNAR 322 Query: 313 FASGYPALEASVQGIYSD 330 AS + Sbjct: 323 QASQLAQSASDTAQHGGK 340 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,304,711 Number of extensions: 1131679 Number of successful extensions: 2908 Number of sequences better than 1.0e-05: 37 Number of HSP's gapped: 2852 Number of HSP's successfully gapped: 37 Length of query: 338 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 231 Effective length of database: 26,037,693 Effective search space: 6014707083 Effective search space used: 6014707083 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)