BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0554.0005.p (159 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SA0183_3 [G] COG2190 Phosphotransferase system IIA components 120 8e-28 BS_ybfS_3 [G] COG2190 Phosphotransferase system IIA components 115 4e-26 BH0844_3 [G] COG2190 Phosphotransferase system IIA components 114 4e-26 SPy0572_3 [G] COG2190 Phosphotransferase system IIA components 114 7e-26 L90678_3 [G] COG2190 Phosphotransferase system IIA components 111 4e-25 >SA0183_3 [G] COG2190 Phosphotransferase system IIA components Length = 143 Score = 120 bits (301), Expect = 8e-28, Method: Compositional matrix adjust. Identities = 61/120 (50%), Positives = 85/120 (70%), Gaps = 1/120 (0%) Query: 20 VPGQVVGLEQVPDEAFSTKAMGEGFAVQPIKGEVRAPFSGKISHIMNRSNHAILLENEAG 79 + G+V L +VPD+ FS K MG+G A++P +GEVRAPF+GKI I + HAI L +++G Sbjct: 1 LTGEVTPLSEVPDQVFSEKMMGDGIAIKPSQGEVRAPFNGKIQMIF-PTKHAIGLVSDSG 59 Query: 80 IQILIHVGVDTVSLKGEGFVPHVVTGQTVEQGELLLEFDIARIQEAGLSDITSVIVPGGQ 139 +++LIH+G+DTV L GEGF HV GQ V+QG+LL+ FD+ I+ SDIT +IV G Sbjct: 60 LELLIHIGLDTVKLNGEGFTLHVEEGQEVKQGDLLINFDLDYIRNHAKSDITPIIVTQGN 119 >BS_ybfS_3 [G] COG2190 Phosphotransferase system IIA components Length = 137 Score = 115 bits (287), Expect = 4e-26, Method: Compositional matrix adjust. Identities = 63/126 (50%), Positives = 80/126 (63%), Gaps = 1/126 (0%) Query: 16 IISPVPGQVVGLEQVPDEAFSTKAMGEGFAVQPIKGEVRAPFSGKISHIMNRSNHAILLE 75 I P+ G+ V L VPD+ FS K MGEGFA+ P +G+V AP G+I I + HAI Sbjct: 5 FIYPIKGETVSLGDVPDQVFSEKMMGEGFAIIPSEGKVVAPADGEIVSIF-PTKHAIGFM 63 Query: 76 NEAGIQILIHVGVDTVSLKGEGFVPHVVTGQTVEQGELLLEFDIARIQEAGLSDITSVIV 135 + G +ILIHVG+DTV L GEGF HV +GQ V+QGELLL FD+ I++ S IT VI Sbjct: 64 SAGGTEILIHVGIDTVKLNGEGFEAHVTSGQAVKQGELLLTFDLNYIKQHAASAITPVIF 123 Query: 136 PGGQEK 141 E+ Sbjct: 124 TNTSEE 129 >BH0844_3 [G] COG2190 Phosphotransferase system IIA components Length = 151 Score = 114 bits (286), Expect = 4e-26, Method: Compositional matrix adjust. Identities = 57/116 (49%), Positives = 78/116 (67%), Gaps = 1/116 (0%) Query: 19 PVPGQVVGLEQVPDEAFSTKAMGEGFAVQPIKGEVRAPFSGKISHIMNRSNHAILLENEA 78 P+ G+++ L VPDE FS K MG+GFA++P +G V APF GK+S I + HAI L + Sbjct: 7 PIEGKLLDLSDVPDEVFSKKMMGDGFAIEPTEGIVYAPFDGKVSTIF-PTKHAIGLTTDT 65 Query: 79 GIQILIHVGVDTVSLKGEGFVPHVVTGQTVEQGELLLEFDIARIQEAGLSDITSVI 134 G ++LIH+G+DTV+LKGEGF HV V+QG+ L+ FD+ I E S IT +I Sbjct: 66 GKEVLIHIGIDTVNLKGEGFTAHVEQDDIVQQGQKLMSFDLDVINEKAKSAITPII 121 >SPy0572_3 [G] COG2190 Phosphotransferase system IIA components Length = 172 Score = 114 bits (285), Expect = 7e-26, Method: Compositional matrix adjust. Identities = 64/149 (42%), Positives = 96/149 (64%), Gaps = 10/149 (6%) Query: 16 IISPVPGQVVGLEQVPDEAFSTKAMGEGFAVQPIKGEVRAPFSGKISHIMNRSNHAILLE 75 + +P+ G+++ L +VPDE FS+K +GEGFA+ P +GEV APF G++ + HA+ L+ Sbjct: 26 LYAPMTGEMLFLSEVPDETFSSKLLGEGFAILPSEGEVYAPFDGEVITFFP-TKHAVALK 84 Query: 76 NEAGIQILIHVGVDTVSLKGEGFVPHVVTGQTVEQGELLLEFDIARIQEAGLSDITSVIV 135 N G+++LIHVG+DTV LKG+GF V G V++G+ LL+ DI I G S I+ V+V Sbjct: 85 NTRGVEVLIHVGIDTVELKGQGFEQLVSVGDVVKRGQALLKMDIDFITSKGYSLISPVVV 144 Query: 136 PGGQEKVQRIEGLQD-------EDAVLRI 157 E+++ I +QD EDA+L I Sbjct: 145 TNSAEQLEII--IQDDKKMVTKEDALLVI 171 >L90678_3 [G] COG2190 Phosphotransferase system IIA components Length = 166 Score = 111 bits (278), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 59/123 (47%), Positives = 83/123 (67%), Gaps = 1/123 (0%) Query: 18 SPVPGQVVGLEQVPDEAFSTKAMGEGFAVQPIKGEVRAPFSGKISHIMNRSNHAILLENE 77 SP+ G+V+ L QV D AFS MG+G A++P GEVRAPF G + I+ + HA+ L + Sbjct: 11 SPLEGKVIPLSQVKDAAFSAGVMGKGAAIEPTLGEVRAPFDGMV-MILFPTKHAVGLISN 69 Query: 78 AGIQILIHVGVDTVSLKGEGFVPHVVTGQTVEQGELLLEFDIARIQEAGLSDITSVIVPG 137 G ++LIH+G+DTV L+G+ F V GQ+V++G+LLL+FDI RIQ AG S +IV Sbjct: 70 EGTELLIHIGIDTVQLEGKYFETFVKQGQSVKKGDLLLKFDIERIQNAGYSTQVPIIVTN 129 Query: 138 GQE 140 Q+ Sbjct: 130 TQD 132 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.138 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 12,103,791 Number of extensions: 442795 Number of successful extensions: 964 Number of sequences better than 1.0e-05: 49 Number of HSP's gapped: 918 Number of HSP's successfully gapped: 49 Length of query: 159 Length of database: 38,378,431 Length adjustment: 98 Effective length of query: 61 Effective length of database: 27,075,699 Effective search space: 1651617639 Effective search space used: 1651617639 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 111 (47.4 bits)