BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0554.0002.p (489 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SA0186_2 [G] COG1263 Phosphotransferase system IIC components, g... 362 e-100 L145238_2 [G] COG1263 Phosphotransferase system IIC components, ... 350 2e-96 STM2570_2 [G] COG1263 Phosphotransferase system IIC components, ... 225 1e-58 BS_ybbF_2 [G] COG1263 Phosphotransferase system IIC components, ... 192 1e-48 PM1575_2 [G] COG1263 Phosphotransferase system IIC components, g... 167 5e-41 >SA0186_2 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 355 Score = 362 bits (928), Expect = e-100, Method: Compositional matrix adjust. Identities = 199/351 (56%), Positives = 256/351 (72%) Query: 137 KSISNIFIPLAPAFVAAGLLAGITSVIGNFITAGHLSGEGWAQFNLILNVIKNGIFSYMA 196 KSI+NIFIPL PAF+ AGL+ GI +V+ N + AG++SG Q + NVIK+G+ +Y+A Sbjct: 1 KSIANIFIPLIPAFIGAGLIGGIAAVLSNLMVAGYISGAWITQLITVFNVIKDGMLAYLA 60 Query: 197 IFVGINSARVFGASPSLGGVIGGATLLTGMKPELPIHNLFTGEPLIAGQGGIIGVVLAVW 256 IF GIN+A+ FGA+P LGGVIGG TLLTG+ + + N+FTGEPL GQGGIIGV+ AVW Sbjct: 61 IFTGINAAKEFGATPGLGGVIGGTTLLTGIAGKNILMNVFTGEPLQPGQGGIIGVIFAVW 120 Query: 257 ILALIEKRLHKIMPNSIDLIVTSFLSVLATGLLTIFLIMPLAGFVADNLLVAVNWILSVX 316 IL+++EKRLHKI+PN+ID+IVT +++L GLLTIF+ MPLAGFV+D+L+ VN I+S+ Sbjct: 121 ILSIVEKRLHKIVPNAIDIIVTPTIALLIVGLLTIFIFMPLAGFVSDSLVSVVNGIISIG 180 Query: 317 XXXXXXXXXXIWLVMVMLGLHHIMTPIHIELIAQTGMTILLPIXXXXXXXXXXXXXXXWI 376 +L +VMLGLHHI TPIHIE+I Q+G T LLPI W+ Sbjct: 181 GVFSGFIIGASFLPLVMLGLHHIFTPIHIEMINQSGATYLLPIAAMAGAGQVGAALALWV 240 Query: 377 RCRKNKQLTGIIKGALPVGVLGIGEPLIYGVTLPLGKPFVTACLGGAFGGAVIGYFGNVG 436 RC++N L +KGALPVG LGIGEPLIYGVTLPLG+PF+TAC+GG GGAVIG G++G Sbjct: 241 RCKRNTTLRNTLKGALPVGFLGIGEPLIYGVTLPLGRPFLTACIGGGIGGAVIGGIGHIG 300 Query: 437 AMSIGASGIEMLLLIADGKWWVYLLGLITAYFFGFICTYLFGTPKEARQAK 487 A +IG SG+ +L LI+D + Y+ GL+ AY GF+CTYLFGT K RQ Sbjct: 301 AKAIGPSGVSLLPLISDNMYLGYIAGLLAAYAGGFVCTYLFGTTKAMRQTD 351 >L145238_2 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 348 Score = 350 bits (899), Expect = 2e-96, Method: Compositional matrix adjust. Identities = 186/334 (55%), Positives = 244/334 (73%) Query: 133 KNFLKSISNIFIPLAPAFVAAGLLAGITSVIGNFITAGHLSGEGWAQFNLILNVIKNGIF 192 K L +IS IF+PL PAFV AGL+ G+ SV+GN +TAG L W QF +L +I++GIF Sbjct: 15 KAVLNTISKIFVPLIPAFVGAGLIGGLASVLGNLVTAGTLDAATWTQFITVLKIIQSGIF 74 Query: 193 SYMAIFVGINSARVFGASPSLGGVIGGATLLTGMKPELPIHNLFTGEPLIAGQGGIIGVV 252 SY+AI+VGINSA+ FGA+PSLGGVIG +LLTGM PE P+ N+F G L AGQGGIIGV+ Sbjct: 75 SYLAIYVGINSAQEFGATPSLGGVIGAVSLLTGMNPEAPLKNIFNGSALSAGQGGIIGVI 134 Query: 253 LAVWILALIEKRLHKIMPNSIDLIVTSFLSVLATGLLTIFLIMPLAGFVADNLLVAVNWI 312 AVW+L+++EK+L K +P+SID+IVT + +L G IF IMP+AG ++ +L+ A+N+I Sbjct: 135 FAVWLLSILEKQLRKFIPDSIDIIVTPTIGLLVIGFAEIFAIMPVAGVISSSLVGAINFI 194 Query: 313 LSVXXXXXXXXXXXIWLVMVMLGLHHIMTPIHIELIAQTGMTILLPIXXXXXXXXXXXXX 372 L V ++L MVM GLH I+TPIH+E+IA+T T LLPI Sbjct: 195 LQVGGGFSGFVLGVLFLPMVMFGLHQILTPIHLEMIAKTCSTQLLPILAMAGAGQVGAAI 254 Query: 373 XXWIRCRKNKQLTGIIKGALPVGVLGIGEPLIYGVTLPLGKPFVTACLGGAFGGAVIGYF 432 W+R RK+K+ ++KGALPVG+LGIGEPLIYG+TLPLG+PF+TAC+GG GGA+IG Sbjct: 255 ALWLRLRKDKEFVELVKGALPVGILGIGEPLIYGITLPLGRPFITACIGGGIGGAIIGSL 314 Query: 433 GNVGAMSIGASGIEMLLLIADGKWWVYLLGLITA 466 G GA++IG SG+ +L LIA+GKWWVYLLGL+ A Sbjct: 315 GQAGAIAIGPSGLALLPLIANGKWWVYLLGLLGA 348 >STM2570_2 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 357 Score = 225 bits (573), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 136/355 (38%), Positives = 207/355 (58%), Gaps = 9/355 (2%) Query: 130 TPVKNFLKSISNIFIPLAPAFVAAGLLAGITSVIGNFITAGHLSGEGWAQFNLILNVIKN 189 P+ + L+ ++N+FIPL PAF+A+GL+ GI +++ G ++ + ++ + + Sbjct: 10 APMSDALRKLANVFIPLIPAFIASGLITGIINILKRPDIVGDVAVH-YPNLLGLMGIFGS 68 Query: 190 GIFSYMAIFVGINSARVFGASPSLGGVIGGATLLTGMKPELPIHNLFTGEPLIAGQGGII 249 +F+ M I VG+N+A+VFG S +LGGV+ G P+L LF GE L G+GG+I Sbjct: 69 AVFAIMNILVGVNTAKVFGGSQALGGVMAGIL----SSPQLAQITLF-GEVLQPGRGGVI 123 Query: 250 GVVLAVWILALIEKRLHKIMPNSIDLIVTSFLSVLATGLLTIFLIMPLAGFVADNLLVAV 309 V+L V ++ IE++ K++P S++LI+ L+ + TG + I + PL G+++D + Sbjct: 124 AVLLVVALMCWIERQFRKLLPGSLELILNPLLTTVITGAVAIVALQPLGGWISDAIAHGA 183 Query: 310 NWILSVXXXXXXXXXXXIWLVMVMLGLHHIMTPIHIELIAQTGMTILLPIXXXXXXXXXX 369 +W + +L +V+ GLH + PIH+EL+ G L PI Sbjct: 184 SWAIDRGGFLVGAVLAGTFLPLVLTGLHQGLVPIHVELVQAHGYNALFPILAMAGVGQIG 243 Query: 370 XXXXXWIRCRKNKQLTGIIKGALPVGVLGIGEPLIYGVTLPLGKPFVTACLGGAFGGAVI 429 ++ R N +L +IKGALPVG+LGIGEPLI+GVTLPLGKPF+ ACLGGA GGA+I Sbjct: 244 AAIAVLMKTR-NARLKKVIKGALPVGLLGIGEPLIFGVTLPLGKPFIGACLGGAVGGALI 302 Query: 430 GYFGNVGAMSIGASGIEMLLLIADGKWWVYLLGLITAYFFGFICTYL--FGTPKE 482 Y+ ++ G SG+ + L I GK YLLG + A GFI T+L F P+E Sbjct: 303 SYWKVATVITFGISGLPLALTIVAGKVLFYLLGYLIAVIAGFIFTWLLGFNDPEE 357 >BS_ybbF_2 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 301 Score = 192 bits (487), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 20/285 (7%), Positives = 56/285 (19%), Gaps = 11/285 (3%) Query: 130 TPVKNFLKSISNIFIPLAPAFVAAGLLAGITSVIGNFITAGHLSGEGWAQFNLILNVIKN 189 + + + + + + E + I Sbjct: 11 IAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR---HRIVGIHTE--GAYQAIYVDTPG 65 Query: 190 GIFSYMAIFVGINSARVFGASPSLGGVIGGATLLTGMKPELPIHNLFTGEPLIAGQGGII 249 + + + + VI + P + L G+ +I Sbjct: 66 LHMEEKRAINRLMNKAASSSIGDVELVIF----VVEGTRWTPDDEMV-LNKLREGKAPVI 120 Query: 250 GVVLAVWILALIEKRLHKIMPNSIDLIVTSFLSVLATGLLTIFLIMPLAGFVADNLLVAV 309 V V + L + + + + + A L + I + Sbjct: 121 LAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHLPEATHHF 180 Query: 310 NWILSVXXXXXXXXXXXIWLVMVMLGLHHIMTPIHIELIAQTGMTILLPIXXXXXXXXXX 369 I ++ + + +E+ Sbjct: 181 PEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEIERFVSNERGGYDINGLILVERE 240 Query: 370 XXXXXWIRCRKNKQLTGIIKGALPVGVLGIGEPLIYGVTLPLGKP 414 I + ++ I A P+ + + + Sbjct: 241 GQKKMVIGNKGA-KIKTIGIEARKDMQEMFEAPVHLELWVKVKSG 284 >PM1575_2 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 375 Score = 167 bits (422), Expect = 5e-41, Method: Compositional matrix adjust. Identities = 120/371 (32%), Positives = 182/371 (49%), Gaps = 30/371 (8%) Query: 130 TPVKNFLKSISNIFIPLAPAFVAAGLLAGITSVIGNFITAGHLSGEGW-AQFNLILNVIK 188 + + F + IF PL P F+ AGLL G+ +V+ AG + + + V Sbjct: 8 SSIHQFFAKFATIFTPLIPGFIGAGLLLGLATVLQQAFVAGVENPNAFLVDLIAYMKVFS 67 Query: 189 NGIFSYMAIFVGINSARVFGASPSLGGVIGGATLLTGMKPELP------IHNLF--TGEP 240 G+FS+++I +G N+A+ FG S + G I + + G PE + N F T +P Sbjct: 68 KGLFSFLSILIGYNAAKAFGGS-GVNGAILASLFILGYNPEATKGIYSGLSNFFGLTIDP 126 Query: 241 LIAGQGGIIGVVLAVWILALIEKRLHKIMPNSIDLIVTSFLSVLATGLLTIFLIMPLAGF 300 +G IIGV++A + A +E+ + K +P+S+D+ +TS +++L G T IMP+ + Sbjct: 127 ----RGNIIGVLIAAIVGAKVERWVRKFIPDSLDMALTSTVTLLIMGCFTFLFIMPIGVY 182 Query: 301 VADNLLVAVNWILSVXXX--XXXXXXXXIWLVMVMLGLHHIMTPIHIELIAQTGMTILLP 358 + + + +W+ S ++L+ VMLG+H P++ L+ G L P Sbjct: 183 LFNGM----SWLFSNLNGNPLGTAVLAGLFLISVMLGIHQGFVPVYFALVETQGFNALFP 238 Query: 359 IXXXXXXXXXXXXXXXWIRCRKNKQLTGIIKGALPVGVLGIGEPLIYGVTLPLGKPFVTA 418 + + + K L IKGA+ G LGIGEPLIYGVTLP KPF+TA Sbjct: 239 VLAMAGAGQVGAALALYFKANKGAVLRDQIKGAIIPGFLGIGEPLIYGVTLPRVKPFITA 298 Query: 419 CLGGAFGGAVIGYFGNVG-----AMSIGASGIEMLLLIADGKW-----WVYLLGLITAYF 468 C+GGA GG IG +G G SG+ + L+ YLLG + AY Sbjct: 299 CIGGAAGGFTIGLIAYLGFPMGLNTVFGPSGLLAIPLMTSPNGVLPAIATYLLGTVVAYA 358 Query: 469 FGFICTYLFGT 479 GFI TY F T Sbjct: 359 TGFITTYFFAT 369 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.324 0.143 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 34,743,925 Number of extensions: 1474434 Number of successful extensions: 5486 Number of sequences better than 1.0e-05: 78 Number of HSP's gapped: 5350 Number of HSP's successfully gapped: 79 Length of query: 489 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 379 Effective length of database: 25,691,691 Effective search space: 9737150889 Effective search space used: 9737150889 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 118 (50.1 bits)