BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0553.0009.p (239 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value L0185 [IQR] COG1028 Dehydrogenases with different specificities ... 135 6e-32 DR1467 [IQR] COG1028 Dehydrogenases with different specificities... 128 8e-30 all0475 [R] COG4221 Short-chain alcohol dehydrogenase of unknown... 125 6e-29 MA2686 [IQR] COG1028 Dehydrogenases with different specificities... 124 1e-28 all0968 [IQR] COG1028 Dehydrogenases with different specificitie... 119 6e-27 >L0185 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 243 Score = 135 bits (340), Expect = 6e-32, Method: Compositional matrix adjust. Identities = 83/220 (37%), Positives = 127/220 (57%), Gaps = 9/220 (4%) Query: 1 MDLRGRSIVITGAGKGIGKALAMALAKEGANLGLISRTAADLEALKSALTEVYDIKASIA 60 M+++ +++ +TG+ +GIGKA+A+ AK G+NL + R+A E L A Y +KA Sbjct: 1 MEIKNKNVFVTGSTRGIGKAIALQFAKAGSNLIINGRSAISEELL--AEFTAYGVKAVGI 58 Query: 61 VADIAVREDAERAVVSLQKDLGAFDALINNAGIAKFGTFLEMDPADWEHHMQINLFGTYY 120 DI+ EDA++ V + LG+ D L+NNAGI + G L+M D+E ++INL G + Sbjct: 59 SGDISKSEDAKQMVAEAIETLGSVDILVNNAGITRDGLSLKMSEEDFESVLKINLTGAFN 118 Query: 121 VTRAALPAMIERSSGNIINISSTAGERGFATGSAYCASKFALMGMTEALAQEVRKHNIRV 180 +T+A L M SG IINISS G G A + Y ASK L+G+T+++A+EV N+RV Sbjct: 119 MTQAVLKPMTRARSGAIINISSVVGLMGNAGQANYAASKAGLIGLTKSIAREVAARNVRV 178 Query: 181 VALTPSTVNTGLAENAGLKIGDE-------DRMMQPEDVA 213 A+ P + + + E K+ D R PE++A Sbjct: 179 NAVAPGFIESDMTEVLSDKVKDAMKGQIPMKRFGMPEEIA 218 >DR1467 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 255 Score = 128 bits (322), Expect = 8e-30, Method: Compositional matrix adjust. Identities = 78/239 (32%), Positives = 128/239 (53%), Gaps = 17/239 (7%) Query: 5 GRSIVITGAGKGIGKALAMALAKEGANLGLISRTAADLEALKSALTE-----VYDIKASI 59 G+S +TGA KGIG A+A ALA EG + + SR +++ L E V D+K + Sbjct: 23 GKSAFVTGASKGIGYAVAQALAAEGYRVTITSRKQGEIDEAAQQLGENVRGVVCDVKDAA 82 Query: 60 AVADIAVREDAERAVVSLQKDLGAFDALINNAGIAKFGTFLEMDPADWEHHMQINLFGTY 119 AV + AV + + G+ D L NAG+ F ++ W+ + NL G + Sbjct: 83 AV---------QSAVDAHVEAFGSLDVLFVNAGVGHFANIEQLSIEQWQDVIDTNLSGAF 133 Query: 120 YVTRAALPAMIERSSGNIINISSTAGERGFATGSAYCASKFALMGMTEALAQEVRKHNIR 179 Y +AA+PA+ ++ G I+ +SS AG+ F G+AY ASKF L G++E + ++R+++I+ Sbjct: 134 YTIKAAIPAL-KKQGGYILTLSSLAGKNPFEGGAAYNASKFGLNGLSEVVNLDLRQYDIK 192 Query: 180 VVALTPSTVNTGLAENAGLKIGDEDRMMQPEDVAELALAALKLPDRVFLKTAGIWTTNP 238 + P TV T ++ + D+ +QPED+A+L + LK+P R + + P Sbjct: 193 TTQIMPGTVATHFNDHTPNE--DDFWKIQPEDIAQLTVDLLKMPARTLPSRVEVRPSRP 249 >all0475 [R] COG4221 Short-chain alcohol dehydrogenase of unknown specificity Length = 257 Score = 125 bits (314), Expect = 6e-29, Method: Compositional matrix adjust. Identities = 75/226 (33%), Positives = 119/226 (52%), Gaps = 14/226 (6%) Query: 1 MDLRGRSIVITGAGKGIGKALAMALAKEGANLGLISRTAADLEALKSALTEVYDIKASIA 60 + L+ + ++ITGA GIG A A A GA L L +R A L+ L L + + ++ + Sbjct: 2 ISLKNQIVLITGASSGIGNACARIFAGAGAKLILAARRLARLQQLADELNQDFGVEIHLL 61 Query: 61 VADIAVREDAERAVVSLQKDLGAFDALINNAGIAK-FGTFLEMDPADWEHHMQINLFGTY 119 D+ R E A+ SL A D LINNAG+++ E D DWE + N+ G Sbjct: 62 QLDVRDRPSVESAIASLPPAWSAIDILINNAGLSRGLDKLHEGDLQDWEEMIDTNVKGLL 121 Query: 120 YVTRAALPAMIERSSGNIINISSTAGERGFATGSAYCASKFALMGMTEALAQEVRKHNIR 179 Y+TR +P M+ R G++IN+ S AG + + G+ YC +K A+ ++E L Q++ +R Sbjct: 122 YLTRYVVPGMVNRGRGHVINLGSIAGHQTYPGGNVYCGTKAAVKAISEGLKQDLLGTPVR 181 Query: 180 VVALTPSTVNTGLAENAGLKI-GDEDRM---------MQPEDVAEL 215 V ++ P V T +E ++ GD +R + PEDVA++ Sbjct: 182 VTSVDPGMVETEFSE---VRFHGDTERAKKVYQGVNPLTPEDVADV 224 >MA2686 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 239 Score = 124 bits (312), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 87/228 (38%), Positives = 135/228 (59%), Gaps = 12/228 (5%) Query: 1 MDLRGRSIVITGAGKGIGKALAMALAKEGANLGLISRTAADLEALKSALTEVYDIKASIA 60 M LRGR VITG G+GIG+A+ +ALA+EGA++ + +RT ++ + + E KA Sbjct: 4 MKLRGRIAVITGGGRGIGRAICLALAREGADIVIAARTEKEIRK-TARMVERVGGKALAV 62 Query: 61 VADIAVREDAERAVVSLQKDLGAFDALINNAGIAKFGTFL-EMDPADWEHHMQINLFGTY 119 DI E+ + + K G D LINNAG+A +G +L + ++E M N+ G + Sbjct: 63 GTDIRKEEEVRKMISEAVKTFGKIDILINNAGVA-YGKYLVDTSTEEYETIMDTNVKGMF 121 Query: 120 YVTRAALPAMIERSSGNIINISSTAGERGFATGSAYCASKFALMGMTEALAQEVRKHNIR 179 + T+ ALP +++R G IINISS AG+ G S YCASKFA++G TE++A E+ ++ Sbjct: 122 FCTKYALPHLLKRGEGRIINISSGAGKHGIPKLSIYCASKFAVIGFTESVAYEI-AGGLQ 180 Query: 180 VVALTPSTVNTGLAENAGLKIGDEDRMMQPEDVA----ELALAALKLP 223 V A+ P++V+T + + + + +++PEDVA EL L LP Sbjct: 181 VYAVCPASVDTDMYHS----LHSDKPVLKPEDVARKVLELCLPETTLP 224 >all0968 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 293 Score = 119 bits (297), Expect = 6e-27, Method: Compositional matrix adjust. Identities = 73/224 (32%), Positives = 127/224 (56%), Gaps = 8/224 (3%) Query: 1 MDLRGRSIVITGAGKGIGKALAMALAKEGAN-LGLISRTAADLEALKSALTEV--YDIKA 57 M++RG+ ++TGA +GIG+A+A+ LA++G + L L++R D + L+ EV ++A Sbjct: 1 MNIRGKVALVTGASRGIGRAIALELAQQGIHRLILVAR---DRQKLREVAQEVEAMGVEA 57 Query: 58 SIAVADIAVREDAERAVVSLQKDLGAFDALINNAGIAKFGTFLEMDPADWEHHMQINLFG 117 + D+ + A+ L ++ G L+N AG+A +FL+ + + +NL G Sbjct: 58 TTLAIDLTQATEVNIAIAQLWRNYGPIHLLVNCAGVAYQSSFLQSKLPQVQEELSVNLLG 117 Query: 118 TYYVTRAALPAMIERSSGNIINISSTAGERGFATGSAYCASKFALMGMTEALAQEVRKHN 177 Y +T M + G IIN+SS G+ T + Y A+KFA++G T+AL +E+ ++N Sbjct: 118 MYTLTSLIAKRMASQRQGTIINVSSLMGKVAAPTMATYSATKFAILGFTQALRRELAEYN 177 Query: 178 IRVVALTPSTVNTGLAENAGLKIGDEDRMMQPEDVAELALAALK 221 I+V AL PS +T + + LK+ M P++VA+ +A L+ Sbjct: 178 IQVKALLPSLTDTDMVRD--LKLFRWVIPMTPQEVAKALIAGLE 219 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.132 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,770,541 Number of extensions: 559783 Number of successful extensions: 3502 Number of sequences better than 1.0e-05: 356 Number of HSP's gapped: 3070 Number of HSP's successfully gapped: 356 Length of query: 239 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 136 Effective length of database: 26,499,029 Effective search space: 3603867944 Effective search space used: 3603867944 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 114 (48.5 bits)