BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0551.0004.p (361 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ydfO [E] COG0346 Lactoylglutathione lyase and related lyases 397 e-110 BS_yodE [E] COG0346 Lactoylglutathione lyase and related lyases 260 2e-69 SMa0374 [E] COG0346 Lactoylglutathione lyase and related lyases 216 4e-56 BS_ykcA [E] COG0346 Lactoylglutathione lyase and related lyases 197 2e-50 lin0292 [E] COG0346 Lactoylglutathione lyase and related lyases 184 1e-46 >BS_ydfO [E] COG0346 Lactoylglutathione lyase and related lyases Length = 141 Score = 397 bits (1021), Expect = e-110, Method: Compositional matrix adjust. Identities = 0/312 (0%), Positives = 0/312 (0%) Query: 46 MTMQTQGIHHITAFVGDVQRNADFYAGILGLRLVKKTINFDAPEVYHLYFGNEQGSPGTI 105 M + I + D RN Y ++ + + Sbjct: 1 MEGLSMDQVVIFKQIFDKVRNDLNYQWFYSELKRHNVSHYIYYLATENVHIVLKNDNTVL 60 Query: 106 ITFFPWSXXXXXXXXXXXXXXTTYAVPVGSMDFWKQRLAAYQIPVTEVSRIGESYLAFAD 165 + T+ + + F + R + V + Sbjct: 61 LKGLKNIVSVKFSKDRHLIETTSNKLKSREITFQEYRRNLAKAGVFRWVTNIHEQKRYYY 120 Query: 166 FDGLRIELVEREEGALSTWSFGGVTPEHAIKGFGGAVLYSTNPEKTADTLSRTLGLERIA 225 Sbjct: 121 180 Query: 226 EGDGYIRYRSTGDLGNIIDLKAAPVPHGAGGSGTVHHIAWRAKDDAEQLEWGRRVQSHGY 285 Sbjct: 181 240 Query: 286 QPTPVQDRQYFNAIYFREEGGILFEIATDPPGFARDEAPDALGQKLMLPAWFEPHRAVIE 345 Sbjct: 241 300 Query: 346 ENLTPFEIREIE 357 Sbjct: 301 312 >BS_yodE [E] COG0346 Lactoylglutathione lyase and related lyases Length = 303 Score = 260 bits (665), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 130/301 (43%), Positives = 177/301 (58%), Gaps = 1/301 (0%) Query: 48 MQTQGIHHITAFVGDVQRNADFYAGILGLRLVKKTINFDAPEVYHLYFGNEQGSPGTIIT 107 M+T+G+HH+TAF D Q N FY +LGLRLVKKT+NFD P YH YFGN+ G PGTI+T Sbjct: 1 MKTEGLHHVTAFARDPQENLRFYTEVLGLRLVKKTVNFDDPGTYHFYFGNQNGDPGTIMT 60 Query: 108 FFPWSXXXXXXXXXXXXXXTTYAVPVGSMDFWKQRLAAYQIPVTEVSRIGESYLAFADFD 167 FFP+ ++VP GS+ FWK+RL + + E + GE L F D + Sbjct: 61 FFPFQGSGQGTVGKGQAGRVYFSVPSGSLSFWKERLEKSGLSLEEKTLFGEKGLIFDDTE 120 Query: 168 GLRIELVEREEGALSTWSFGGVTPEHAIKGFGGAVLYSTNPEKTADTLSRTLGLERIAEG 227 L + ++E + S W+ G+T AI G G +LYS +P+ T L+ + G ++AE Sbjct: 121 DLPLAIMEDAKSGKSEWTPDGITTNEAITGMKGVLLYSYDPQATIQLLTESFGYTKVAEE 180 Query: 228 DGYIRYRSTGDLGNIIDLKAAPVPHGAGGSGTVHHIAWRAKDDAEQLEWGRRVQSHGYQP 287 D +R S+ +G +ID+ P G GG GTVHH+A+R K EQ +W + + Sbjct: 181 DQIVRLASSAAVGGVIDVHLHPEKRGVGGYGTVHHVAFRTK-KKEQAKWLPIIAENHLPS 239 Query: 288 TPVQDRQYFNAIYFREEGGILFEIATDPPGFARDEAPDALGQKLMLPAWFEPHRAVIEEN 347 + + DR+YF ++YFRE+GGILFEIATD PGF DE LG L LP W E HR I + Sbjct: 240 SEILDREYFTSVYFREKGGILFEIATDEPGFMTDETFAELGTSLKLPEWLEKHRQQITDI 299 Query: 348 L 348 L Sbjct: 300 L 300 >SMa0374 [E] COG0346 Lactoylglutathione lyase and related lyases Length = 310 Score = 216 bits (551), Expect = 4e-56, Method: Compositional matrix adjust. Identities = 121/309 (39%), Positives = 170/309 (55%), Gaps = 4/309 (1%) Query: 46 MTMQTQGIHHITAFVGDVQRNADFYAGILGLRLVKKTINFDAPEVYHLYFGNEQGSPGTI 105 M +G+HH+T+ D ++N F+ LGLR VK+T+NFD P VYHLY+G+E GS GT+ Sbjct: 1 MIKDIKGLHHVTSMASDARQNNRFFTDTLGLRRVKQTVNFDDPSVYHLYYGDETGSAGTV 60 Query: 106 ITFFPWSXXXXXXXXXXXXXXTTYAVPVGSMDFWKQRLAAYQIPVTEVSRI-GESYLAFA 164 +T+FP+ T ++VP GS+ FW+ R + E + G L F Sbjct: 61 MTYFPFPNMMLGRPGVGEVGETQFSVPKGSLKFWQDRFTTQGVDGLERDTVFGADRLRFM 120 Query: 165 DFDGLRIELVEREEGALSTWSFGGVTPEHAIKGFGGAVLYSTNPEKTADTLSRTLGLERI 224 DG L+E + + W G+ + AI+GF GA +S + + L +G ER Sbjct: 121 GPDGDSFALIESADDKRAPWLADGIPDDAAIRGFAGA-RFSLHDSAATEELLGFMGYERA 179 Query: 225 AEGDGYIRYR-STGDLGNIIDLKAAP-VPHGAGGSGTVHHIAWRAKDDAEQLEWGRRVQS 282 + +R+ S G+ + IDL A P P G+G+VHHIA+ + +QLE + + Sbjct: 180 EKEGDVVRFIISNGNGADTIDLLALPKTPFARQGAGSVHHIAFAVDNREKQLEVRKALMD 239 Query: 283 HGYQPTPVQDRQYFNAIYFREEGGILFEIATDPPGFARDEAPDALGQKLMLPAWFEPHRA 342 GYQ TPV DR YF AIYFR GGILFE+AT+ PGF RDE LG+ L LP +E +R Sbjct: 240 TGYQVTPVIDRDYFWAIYFRTPGGILFEVATNEPGFNRDEDTAHLGEALKLPVRYEEYRG 299 Query: 343 VIEENLTPF 351 I+ NL P Sbjct: 300 QIQANLVPL 308 >BS_ykcA [E] COG0346 Lactoylglutathione lyase and related lyases Length = 316 Score = 197 bits (501), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 114/316 (36%), Positives = 164/316 (51%), Gaps = 11/316 (3%) Query: 48 MQTQGIHHITAFVGDVQRNADFYAGILGLRLVKKTINFDAPEVYHLYFGNEQGSPGTIIT 107 M+ GIHH++A D Q+N DFY +LGL+LVKK++N D P +YHL++G+E +PGT +T Sbjct: 1 MKVNGIHHVSALTADAQKNLDFYKKVLGLKLVKKSVNQDEPTMYHLFYGDEVANPGTELT 60 Query: 108 FFPWSXXXXXXXXXXXXXXTTYAVP-VGSMDFWKQRLAAYQIPVTEVS-RIGESYLAFAD 165 FF VP ++ +WK+R Q+ + +S R G LAF D Sbjct: 61 FFEIPRIAPFHAGTNSISSIGLRVPGTEALHYWKERFEEQQVTHSGISKRAGRDILAFQD 120 Query: 166 FDGLRIELVEREEGALSTWSF--GGVTPEHAIKGFGGAVLYSTNPEKTADTLSRTLGLER 223 +G R+ L EEG G+ E + +G G L E T L+ LG Sbjct: 121 HEGQRLVLTADEEGKGYGLPVKQSGIPEEFSFRGLGPVELTVPYAEPTLHVLTNILGFTE 180 Query: 224 IA----EGDGYIRYRSTGDLGNIIDL---KAAPVPHGAGGSGTVHHIAWRAKDDAEQLEW 276 I+ EG G +G+ G ++ + +P G G+VHH+A+R +D+ E W Sbjct: 181 ISREPVEGQGTAVILESGEGGAATEIHLIERNDLPRERQGKGSVHHVAFRVRDEEELAGW 240 Query: 277 GRRVQSHGYQPTPVQDRQYFNAIYFREEGGILFEIATDPPGFARDEAPDALGQKLMLPAW 336 R + G+ + + +R YF A+YFRE GILFE++TD PGF DE D LGQ + LP + Sbjct: 241 HRIISREGFSNSGIVERYYFKALYFREPNGILFELSTDGPGFMVDENLDELGQTIALPPY 300 Query: 337 FEPHRAVIEENLTPFE 352 E RA IE L P + Sbjct: 301 LEHRRAEIEAKLKPIQ 316 >lin0292 [E] COG0346 Lactoylglutathione lyase and related lyases Length = 319 Score = 184 bits (468), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 111/317 (35%), Positives = 165/317 (52%), Gaps = 13/317 (4%) Query: 48 MQTQGIHHITAFVGDVQRNADFYAGILGLRLVKKTINFDAPEVYHLYFGNEQGSPGTIIT 107 M+ GIHH++ F + + N DFY I+GLRLVKK++N D P YHLY+G+E GSPGT +T Sbjct: 1 MKLAGIHHVSIFTANARANFDFYTKIMGLRLVKKSVNQDDPYTYHLYYGDEIGSPGTALT 60 Query: 108 FFPWSXXXXXXXXXXXXXXTTYAVPVG-SMDFWKQRLAAYQIPVTE-VSRIGESYLAFAD 165 FF + VP ++ +W +RL ++I ++ + + G + D Sbjct: 61 FFEVPNMAKNHPSRNAISGLSLRVPNDEALHYWDKRLDEHRIFHSKPIDQFGRKIIRLKD 120 Query: 166 FDGLRIELVEREEGA----LSTWSFGGVTPEHAIKGFGGAVLYSTNPEKTADTLSRTLGL 221 DGL + L+ E +S W V E+AI+G G EKT L++ LG Sbjct: 121 TDGLPVSLISDETSTQFTEVSAWEDSPVPAEYAIRGLGPVRFSVFKKEKTDRLLTKILGF 180 Query: 222 ERIA---EGDGYIRYRSTGD--LGNIIDLKAAP-VPHGAGGSGTVHHIAWRAKDDAEQLE 275 ERI + D + TGD LG + +++ P + G G+G +HH+A+R D + + Sbjct: 181 ERIGAYEDDDKLVTVFKTGDVGLGGEVHVESRPDLEQGNLGAGGIHHVAFRVPTDGDLIG 240 Query: 276 WGRRVQSHGYQPTPVQDRQYFNAIYFREEGGILFEIATDPPGFARDEAPDALGQKLMLPA 335 W +Q GY+ + DR YF+++YFRE GIL E+ATD PGF D + G + LP Sbjct: 241 WTEMIQDLGYKNSGYVDRFYFHSLYFRESNGILIELATDEPGFQTDFTKEH-GTYVDLPP 299 Query: 336 WFEPHRAVIEENLTPFE 352 E R I +L P + Sbjct: 300 HLEERREDILAHLKPLD 316 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.139 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,679,196 Number of extensions: 1240737 Number of successful extensions: 1869 Number of sequences better than 1.0e-05: 12 Number of HSP's gapped: 1847 Number of HSP's successfully gapped: 12 Length of query: 361 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 253 Effective length of database: 25,922,359 Effective search space: 6558356827 Effective search space used: 6558356827 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)