BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0548.0041.p (629 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MA2013_2 [T] COG0642 Signal transduction histidine kinase 191 3e-48 PA0928_1 [T] COG0642 Signal transduction histidine kinase 182 1e-45 ZarcB_1 [T] COG0642 Signal transduction histidine kinase 176 1e-43 sll0474_2 [T] COG0642 Signal transduction histidine kinase 170 7e-42 ZbarA_1 [T] COG0642 Signal transduction histidine kinase 170 8e-42 >MA2013_2 [T] COG0642 Signal transduction histidine kinase Length = 296 Score = 191 bits (485), Expect = 3e-48, Method: Compositional matrix adjust. Identities = 105/256 (41%), Positives = 149/256 (58%), Gaps = 14/256 (5%) Query: 248 QKQLLLXXXXXXXXXXXXSEFLAMMSHEIRTPMNGVVGMTDVLLDMTDLTKEQRSYVEVI 307 ++QLL +FLA MSHEIRTPMN V+GM ++LL+ T LT EQR Y+++ Sbjct: 4 EEQLLKAKEAAESANKAKGDFLANMSHEIRTPMNAVLGMLEMLLE-TSLTDEQREYLQLA 62 Query: 308 QKSGXXXXXXXXXXXXXSKIEAGRSELQEHNFDLRKCIKDSFSVVSKKADEKKIELSYTV 367 S SKIE + EL++ +F+L I +++S KA K ++L++ + Sbjct: 63 HASAESLLSIIDDVLDFSKIEQNKLELEQISFELESLISHIINLLSGKAGSKGLKLAFHI 122 Query: 368 NHDVPDYIYGDAERLKQVLLNLLGNAVKFTSKGSIAVKVRLLK-------------EEAC 414 D+P GD RLKQVL N+LGNA+KFT KG IA+ V + E Sbjct: 123 EKDLPSTFIGDPVRLKQVLFNILGNAIKFTKKGEIALSVEVYMPSERNSGLSNSDFPEEV 182 Query: 415 RLAFTVTDTGIGIDPDRLDDIFEPFAQIDSFMSRSHEGTGLGLAISRRIVDMMGGEIYAE 474 L F V DTGIGI P++L IF+PF Q D+ ++R + GTGLGLAIS ++V++M G I+ E Sbjct: 183 ALLFKVKDTGIGIPPEKLSQIFDPFIQADASVTREYGGTGLGLAISSQLVELMEGRIWVE 242 Query: 475 SDGKHGSSFTFTIQFK 490 S+ G +F FT++ K Sbjct: 243 SEVGKGCTFYFTVRVK 258 >PA0928_1 [T] COG0642 Signal transduction histidine kinase Length = 509 Score = 182 bits (463), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 96/226 (42%), Positives = 142/226 (62%), Gaps = 3/226 (1%) Query: 266 SEFLAMMSHEIRTPMNGVVGMTDVLLDMTDLTKEQRSYVEVIQKSGXXXXXXXXXXXXXS 325 SEFLA MSHEIRTP+NG++G T+ LL ++L+ Q+ Y+ IQKS S Sbjct: 285 SEFLANMSHEIRTPLNGILGFTN-LLQKSELSPRQQDYLTTIQKSAESLLGIINEILDFS 343 Query: 326 KIEAGRSELQEHNFDLRKCIKDSFSVVSKKADEKKIELSYTVNHDVPDYIYGDAERLKQV 385 KIEAG+ L+ F+LR I+D+ ++++ A EK++EL V D P + GD +RLKQ+ Sbjct: 344 KIEAGKLVLENLPFNLRDLIQDALTMLAPAAHEKQLELVSLVYRDTPIQLQGDPQRLKQI 403 Query: 386 LLNLLGNAVKFTSKGSIAVKVRLLKE--EACRLAFTVTDTGIGIDPDRLDDIFEPFAQID 443 L NL+GNA+KFT G++AV+ L E + +L +V DTGIG+ + +F+ F+Q D Sbjct: 404 LTNLVGNAIKFTQGGTVAVRAMLEDESDDRAQLRISVQDTGIGLSEEDQQALFKAFSQAD 463 Query: 444 SFMSRSHEGTGLGLAISRRIVDMMGGEIYAESDGKHGSSFTFTIQF 489 + +SR GTGLGL IS+R+++ MGGEI +S G+ F ++ Sbjct: 464 NSLSRQAGGTGLGLVISKRLIEQMGGEIGVDSTPGEGAEFWISLSL 509 >ZarcB_1 [T] COG0642 Signal transduction histidine kinase Length = 523 Score = 176 bits (446), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 0/440 (0%), Positives = 0/440 (0%), Gaps = 20/440 (4%) Query: 117 GQL-IDITRQKESERRLQESVERYNSLKKYNHDAVISFGLDGRIINANNMAEKLTGYHIE 175 GQL I+I ++E++ +L++ S + D V D N E LTG E Sbjct: 134 GQLKIEIKEREETQIQLEQQSSFLRSFLDASPDLVFYRNEDKEFSGCNRAMELLTGKS-E 192 Query: 176 TELIGAELAKLIGQANVGRILEKAIYDDSV-EHDIDI-----LVTKEGSTVEVLTSIAPI 229 +L+ + A + +++E D+ V H++ + L +G P Sbjct: 193 KQLVHLKPADVYSPEAAAKVIET---DEKVFRHNVSLTYEQWLDYPDGRKACFEIRKVPY 249 Query: 230 Y-VSGQNIGFYLICKDISEQKQLLLXXXXXXXXXXXXSEFLAMMSHEIRTPMNGVVGMTD 288 Y G+ G +DI+E+K+ + F++ +SHE+RTP+NG+VG++ Sbjct: 250 YDRVGKRHGLMGFGRDITERKRY---QDALERASRDKTTFISTISHELRTPLNGIVGLSR 306 Query: 289 VLLDMTDLTKEQRSYVEVIQKSGXXXXXXXXXXXXXSKIEAGRSELQEHNFDLRKCIKDS 348 +LLD T+LT EQ Y++ I S K+E + +L D + D Sbjct: 307 ILLD-TELTAEQEKYLKTIHVSAVTLGNIFNDIIDMDKMERRKVQLDNQPVDFTSFLADL 365 Query: 349 FSVVSKKADEKKIELSYTVNHDVPDYIYGDAERLKQVLLNLLGNAVKFTSKGSIAVKVRL 408 ++ + +A +K + + +P + D RL+Q+L NL+ NAVKFT +G + V+VR Sbjct: 366 ENLSALQAQQKGLRFNLEPTLPLPHQVITDGTRLRQILWNLISNAVKFTQQGQVTVRVRY 425 Query: 409 LKEEACRLAFTVTDTGIGIDPDRLDDIFEPFAQI-DSFMSRSHEGTGLGLAISRRIVDMM 467 +E L F V D+GIGI D LD IF + Q+ DS + G GL ++ Sbjct: 426 --DEGDMLHFEVEDSGIGIPQDELDKIFAMYYQVKDSHGGKPATGIGLAVSRRLAKNMGG 483 Query: 468 GGEIYAESDGKHGSSFTFTIQFKEASQIPREHFFEEMLHNTGKVNILLAEDNAINQLVMT 527 + +E + T + L Sbjct: 484 DITVTSEQGKGSTFTLTIHAPSVAEEVDDAFDEDDMPL- 542 Query: 528 KMLEKLGHRVTTVPNGKKAI 547 Sbjct: 543 562 >sll0474_2 [T] COG0642 Signal transduction histidine kinase Length = 540 Score = 170 bits (430), Expect = 7e-42, Method: Compositional matrix adjust. Identities = 101/258 (39%), Positives = 150/258 (58%), Gaps = 15/258 (5%) Query: 244 DISE----QKQLLLXXXXXXXXXXXXSEFLAMMSHEIRTPMNGVVGMTDVLLDMT--DLT 297 DI+E ++QL L EFLA MSHE+RTP+N ++GM + L + L Sbjct: 273 DITEIKKAEEQLKLSNKILAKATQLKDEFLANMSHELRTPLNSILGMIETLQNQVYGPLN 332 Query: 298 KEQRSYVEVIQKSGXXXXXXXXXXXXXSKIEAGRSELQEHNFDLRKCIKDSFSVVSKKAD 357 +Q +E+++ SG SKIEAGR +L + + + S + V + A Sbjct: 333 GKQVQSLEIVENSGRHLLSLINDILDLSKIEAGRMDLDLNPTSVSALVNHSLTFVQQFAI 392 Query: 358 EKKIELSYTVNHDVPDYIYGDAERLKQVLLNLLGNAVKFTSKGS-IAVKVRLLKEEA--- 413 +K+I LS + ++PD + D RL QVL+NLL NA+KFT +G + V+ L+KE+ Sbjct: 393 QKQINLSSEIIPNLPDVLV-DKRRLCQVLINLLNNAIKFTPEGGKVVVQADLIKEQGKEN 451 Query: 414 ----CRLAFTVTDTGIGIDPDRLDDIFEPFAQIDSFMSRSHEGTGLGLAISRRIVDMMGG 469 +L+FTV DTGIGI+ DRL++IFEPF QIDS ++R ++GTGLGL++ ++IV++ GG Sbjct: 452 SQSPPQLSFTVFDTGIGIEADRLNNIFEPFVQIDSALNRRYDGTGLGLSLVKKIVELHGG 511 Query: 470 EIYAESDGKHGSSFTFTI 487 + S GS F TI Sbjct: 512 SVTVTSKLNEGSRFKITI 529 >ZbarA_1 [T] COG0642 Signal transduction histidine kinase Length = 525 Score = 170 bits (430), Expect = 8e-42, Method: Compositional matrix adjust. Identities = 90/226 (39%), Positives = 135/226 (59%), Gaps = 3/226 (1%) Query: 266 SEFLAMMSHEIRTPMNGVVGMTDVLLDMTDLTKEQRSYVEVIQKSGXXXXXXXXXXXXXS 325 SEFLA MSHE+RTP+NGV+G T + L T+LT QR ++ I++S S Sbjct: 294 SEFLANMSHELRTPLNGVIGFTRLTLK-TELTPTQRDHLNTIERSANNLLAIINDVLDFS 352 Query: 326 KIEAGRSELQEHNFDLRKCIKDSFSVVSKKADEKKIELSYTVNHDVPDYIYGDAERLKQV 385 K+EAG+ L+ F LR + + ++++ + +K +EL+ + DVPD + GD RL+Q+ Sbjct: 353 KLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTLNIKSDVPDNVIGDPLRLQQI 412 Query: 386 LLNLLGNAVKFTSKGSIAVKV--RLLKEEACRLAFTVTDTGIGIDPDRLDDIFEPFAQID 443 + NL+GNA+KFT G+I + V R L ++ + DTGIGI +F+ F Q D Sbjct: 413 ITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIRDTGIGIPERDQSRLFQAFRQAD 472 Query: 444 SFMSRSHEGTGLGLAISRRIVDMMGGEIYAESDGKHGSSFTFTIQF 489 + +SR H GTGLGL I++++V+ MGG+I S GS+F F I Sbjct: 473 ASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNRGSTFWFHINL 518 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 42,816,686 Number of extensions: 1812184 Number of successful extensions: 6407 Number of sequences better than 1.0e-05: 427 Number of HSP's gapped: 6152 Number of HSP's successfully gapped: 434 Length of query: 629 Length of database: 38,378,431 Length adjustment: 113 Effective length of query: 516 Effective length of database: 25,345,689 Effective search space: 13078375524 Effective search space used: 13078375524 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 119 (50.4 bits)