BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0548.0032.p (156 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM0585 [P] COG4771 Outer membrane receptor for ferrienterocheli... 104 5e-23 TM0585 [R] COG0673 Predicted dehydrogenases and related proteins 104 5e-23 SSO3049 [R] COG0673 Predicted dehydrogenases and related proteins 81 5e-16 SA0210 [R] COG0673 Predicted dehydrogenases and related proteins 79 2e-15 lin2266 [R] COG0673 Predicted dehydrogenases and related proteins 76 2e-14 >STM0585 [P] COG4771 Outer membrane receptor for ferrienterochelin and colicins Length = 751 Score = 104 bits (260), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 14/144 (9%), Positives = 32/144 (22%), Gaps = 6/144 (4%) Query: 7 VAVADTDPVKAAEAAENYGC------EALTDYKQLLGRTDIGIVHLCTPHHLHAPMAAEF 60 A+ + D + A +N A K R I+ +L + Sbjct: 32 AALTNEDTIVVTAAQQNLQAPGVSTITADEIRKNPPARDVSEIIRTMPGVNLTGNSTSGQ 91 Query: 61 LEAGHHVLTEKPIALDVPSARRIQEVAAASQGQLGIVFQNRYNNPSIRIKETIDSGILGR 120 + + + V + + +LG + + + + I Sbjct: 92 RGNNRQIDIRGMGPENTLILIDGKPVTSRNSVRLGWRGERDTRGDTAWVPPEMIERIEVL 151 Query: 121 LICMKGIVTWYRSEDYYTESGWGG 144 + G Sbjct: 152 RGPAAARYGNGAAGGVVNIITKKG 175 >TM0585 [R] COG0673 Predicted dehydrogenases and related proteins Length = 360 Score = 104 bits (260), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 52/144 (36%), Positives = 80/144 (55%), Gaps = 6/144 (4%) Query: 7 VAVADTDPVKAAEAAENYGC------EALTDYKQLLGRTDIGIVHLCTPHHLHAPMAAEF 60 VAV D +A AAE++ E +T+Y++LL R D+ ++ + T H + E Sbjct: 32 VAVCDLVEERANRAAEHFEKSGLRRPETMTNYRELLKREDVDVISIATESGKHYQITMEA 91 Query: 61 LEAGHHVLTEKPIALDVPSARRIQEVAAASQGQLGIVFQNRYNNPSIRIKETIDSGILGR 120 LEAG HVL EKP+AL + E++ +LG+ FQNR+N P +++ +DSG G+ Sbjct: 92 LEAGKHVLVEKPMALSTKHMNEMVELSKQKNLKLGVFFQNRFNPPVQEVRKKLDSGAFGK 151 Query: 121 LICMKGIVTWYRSEDYYTESGWGG 144 + V W R+EDYY ++ W G Sbjct: 152 IFYASVAVRWNRNEDYYKQASWRG 175 >SSO3049 [R] COG0673 Predicted dehydrogenases and related proteins Length = 371 Score = 81.3 bits (199), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 46/137 (33%), Positives = 73/137 (53%), Gaps = 2/137 (1%) Query: 2 EGARLVAVADTDPVKAAEAAENYGCEALTDYKQLLGRTDIGIVHLCTPHHLHAPMAAEFL 61 E +LVAV D A + YG T ++L +++ ++ + TP +LHAP A + Sbjct: 46 EFVKLVAVVDQIKAIAEKIGSEYGTPYYTTIDEVLRNSEVDVISIATPSYLHAPQAILAI 105 Query: 62 EAGHHVLTEKPIALDVPSARRIQEVAAASQGQLGIVFQNRYNNPSIRIKETIDSGILGRL 121 E G HV+ EKP+A + AR + A ++ +LG++FQ RY R+K I LGR+ Sbjct: 106 EYGKHVIVEKPMATTLAGAREMVSRAERNEVKLGVIFQERYAPDIRRLKNDILKE-LGRI 164 Query: 122 ICMKGIVTWYRS-EDYY 137 ++ + WYR + YY Sbjct: 165 YLIESELKWYRDMKGYY 181 >SA0210 [R] COG0673 Predicted dehydrogenases and related proteins Length = 359 Score = 79.3 bits (194), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 50/155 (32%), Positives = 76/155 (49%), Gaps = 13/155 (8%) Query: 1 MEGARLVAVADTDPVKAAEAAENYGCE---ALTDYKQLLGRTDIGIVHLCTPHHLHAPMA 57 +E ++A D D KAA AAE YG + DYK LL I ++H+CTP+ H + Sbjct: 26 VENVEMIAFCDVDISKAASAAEAYGTDNAKVYDDYKALLKDDTIDVIHVCTPNDSHCEIT 85 Query: 58 AEFLEAGHHVLTEKPIALDVPSARRIQEVAAASQGQLGIVFQNRYNNPSIRIKETIDSGI 117 L AG HV+ EKP+A A+++ + A ++ +L I +QNR+ S + + G Sbjct: 86 VAGLHAGKHVMCEKPMAKTTAEAQKMIDTAKSTGKKLTIGYQNRFRPDSQFLHQAAQRGD 145 Query: 118 LGRL-------ICMKGIVTWYRSEDYYTESGWGGG 145 LG + I + + TW + E GGG Sbjct: 146 LGDIYFGKAHAIRRRAVPTWGV---FLNEEAQGGG 177 >lin2266 [R] COG0673 Predicted dehydrogenases and related proteins Length = 358 Score = 75.9 bits (185), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 50/154 (32%), Positives = 78/154 (50%), Gaps = 13/154 (8%) Query: 2 EGARLVAVADTDPVKAAEAAENYGCE---ALTDYKQLLGRTDIGIVHLCTPHHLHAPMAA 58 E A +VA D KA AA+ +G E T+Y +LL I ++H+CTP+ HA ++ Sbjct: 26 EKAEMVAFCDIVLEKAEAAAKEFGSENASVYTNYVELLQDKSIDVIHVCTPNISHAEISI 85 Query: 59 EFLEAGHHVLTEKPIALDVPSARRIQEVAAASQGQLGIVFQNRYNNPSIRIKETIDSGIL 118 +EAG HV+ EKP+A A+ + E A + +L I +QNR+ S + + ++G L Sbjct: 86 AAMEAGKHVMCEKPMAKTTAEAKSMIEAANRTGKKLTIGYQNRFRKDSDYLHQVCENGEL 145 Query: 119 G-------RLICMKGIVTWYRSEDYYTESGWGGG 145 G + I + + TW + E GGG Sbjct: 146 GDIYYAKAKAIRRRAVPTWGV---FLDEEAQGGG 176 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 11,340,297 Number of extensions: 446811 Number of successful extensions: 1016 Number of sequences better than 1.0e-05: 117 Number of HSP's gapped: 1015 Number of HSP's successfully gapped: 117 Length of query: 156 Length of database: 38,378,431 Length adjustment: 97 Effective length of query: 59 Effective length of database: 27,191,033 Effective search space: 1604270947 Effective search space used: 1604270947 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 111 (47.4 bits)