BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0548.0030.p (194 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3346 [G] COG1082 Sugar phosphate isomerases/epimerases 104 6e-23 lin2265 [G] COG1082 Sugar phosphate isomerases/epimerases 94 1e-19 CC1629 [G] COG1082 Sugar phosphate isomerases/epimerases 78 8e-15 mll3313 [G] COG1082 Sugar phosphate isomerases/epimerases 77 2e-14 AGl2948 [G] COG1082 Sugar phosphate isomerases/epimerases 71 1e-12 >BH3346 [G] COG1082 Sugar phosphate isomerases/epimerases Length = 241 Score = 104 bits (260), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 68/196 (34%), Positives = 105/196 (53%), Gaps = 15/196 (7%) Query: 1 VICATHIPWDRLVNDLDALAAEHKLWNCKYVGLGALPGEYQTSQEGYRTFAKKVSETALV 60 V ++H+ +RL N LD + +H C + L E QT+ GY+ + E Sbjct: 59 VPVSSHVALERLENQLDDVIDQHLRLGCHTIYCPYLEEERQTAT-GYKELITSLKEIQKK 117 Query: 61 LKQKHGLQFVYHNHDFEF--ERLDGVTGLEVLLQEADPAAFDFELDLYWVQAGGGSPVDW 118 L++K G+ F YH+HDFE +L+ + + LL E LD+YW+Q G PV+W Sbjct: 118 LEKK-GISFGYHHHDFELYSNQLEAILSIPSLLAE---------LDVYWLQKAGQDPVEW 167 Query: 119 IHRVKDRMRVVHLKDMAVVARKAEFAEIGQGNMNYPAIIEACRETGVEWFVVEQDVCRRD 178 + R K ++R VHLKDM ++ FA +G G +N AI+E+ + VEW++VEQD D Sbjct: 168 VSRYKHKVRHVHLKDMTNDDQEF-FAPVGTGKINIDAILESST-SFVEWWIVEQDDGEGD 225 Query: 179 PFESLKISLDYLHTLL 194 P ++ S Y++ L Sbjct: 226 PLVWIEKSYTYVNERL 241 >lin2265 [G] COG1082 Sugar phosphate isomerases/epimerases Length = 301 Score = 94.0 bits (232), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 9/193 (4%), Positives = 30/193 (15%), Gaps = 8/193 (4%) Query: 2 ICATHIPWDRLVNDLDALAAEHKLWNCKYVGLGALPGEYQTSQEGYRTFAKKVSETALVL 61 I + + + + V Sbjct: 59 IYVDTPGLHMEEKRAINRLMNKAASSSIGD---VELVIFVVEGTRWTPDDEMVLNKLREG 115 Query: 62 KQKHGLQFVYHNHDFEFERLDGVTGLEVLLQEADPAAFDFELDLYWVQAGGGSPVDWIHR 121 K + V + + + + Q + Sbjct: 116 KAP-VILAVNKVDNVQEKADLLPHLQFLASQMNFLDIV--PISAETGLNVDTIAAIVRKH 172 Query: 122 VKDRMRVVHLKDMAVVARKAEFAEIGQGNMNYPAIIEACRETGVEWFVVEQDVCRRDPFE 181 + + + +++ + A V + R ++ Sbjct: 173 LPEATHHFPEDYITDRSQRFMA--SEIIREKLMRFLGAELPYSVTVEIERFVSNERGGYD 230 Query: 182 SLKISLDYLHTLL 194 + L Sbjct: 231 INGLILVEREGQK 243 >CC1629 [G] COG1082 Sugar phosphate isomerases/epimerases Length = 328 Score = 78.2 bits (191), Expect = 8e-15, Method: Compositional matrix adjust. Identities = 50/152 (32%), Positives = 77/152 (50%), Gaps = 20/152 (13%) Query: 54 VSETALVLKQKHGLQFVYHNHDFEFERLDG-----VTGLEVLLQEADPAAFDFELDLYWV 108 ++E A +LK K G+ YHNH+FEF L TG+++LL+ DP+ FELD WV Sbjct: 177 LNEKAAILK-KAGILAGYHNHNFEFAPLKDAKGRETTGMDILLKGTDPSLVTFELDAGWV 235 Query: 109 QAGGGSPVDWIHRVKDRMRVVHLKDMAVVAR-----KAEFAEIGQGNMNYPAIIEACRET 163 A G P + R +H+KD+ + + + E+G G +++ ++ A + Sbjct: 236 TAAGQDPFALLKAYPGRFTQMHVKDVKPSTKPNFELRQDPTEVGSGMIDWKRLLPAAYDA 295 Query: 164 GVEWFVVEQDVCRRDPF-----ESLKISLDYL 190 G+ F EQ+ PF ES +IS DYL Sbjct: 296 GIRGFYYEQE----PPFAYTRLESARISFDYL 323 >mll3313 [G] COG1082 Sugar phosphate isomerases/epimerases Length = 301 Score = 77.0 bits (188), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 6/181 (3%), Positives = 24/181 (13%), Gaps = 23/181 (12%) Query: 6 HIPWDRLVNDLDAL-----AAEHKLWNCKYVGLGALPGEYQTSQEGYRTFAKKVSETALV 60 ++ + + + V + + + V Sbjct: 59 IYVDTPGLHMEEKRAINRLMNKAASSSIGDV----ELVIFVVEGTRWTPDDEMVLNKLRE 114 Query: 61 LKQKHGLQFVYHNHDFEFERL-DGVTGLEVLLQEADPAAFDFELDLYWVQAGGGSPVDWI 119 + + V + + + + + + Sbjct: 115 -GKAPVILAVNKVDNVQEKADLLPHLQFLASQMNF--LDIVPISAETGLNVDTIAAIVRK 171 Query: 120 HRVKDRMRVVHLKDMAVVARKAE------FAEIGQGNMNYPAIIEACR-ETGVEWFVVEQ 172 H + R E + Sbjct: 172 HLPEATHHFPEDYI---TDRSQRFMASEIIREKLMRFLGAELPYSVTVEIERFVSNERGG 228 Query: 173 D 173 Sbjct: 229 Y 229 >AGl2948 [G] COG1082 Sugar phosphate isomerases/epimerases Length = 353 Score = 70.9 bits (172), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 5/151 (3%), Positives = 19/151 (12%), Gaps = 12/151 (7%) Query: 28 CKYVGLGALPGEYQTSQEGYRTFAKKVSETALVLKQKHGLQFVYHNHDFEFERL-DGVTG 86 A + + E Sbjct: 96 GSGR----NDVGASRGHIMDAVEASLKRLQTDHIDLY-QIHATDTLTPVEETLRALDDLV 150 Query: 87 LEVLLQEADPAAFDFELDLYWVQAGGGSPVDWIHRVKDRMRVVHLKDMAVVARKAE---F 143 + + +++ G + + + K Sbjct: 151 RQGKV--RYVGVSNWQAWRIATALGVSARLGLERFNTLQAYYSIAGRDLERELKPLLDAE 208 Query: 144 AEIGQGNMNYPAIIEACR-ETGVEWFVVEQD 173 + + R + + Sbjct: 209 KMGLLVWSPLAGGLLSGRFSRANQSPEGSRR 239 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.139 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,138,611 Number of extensions: 581497 Number of successful extensions: 7114 Number of sequences better than 1.0e-05: 5 Number of HSP's gapped: 7105 Number of HSP's successfully gapped: 5 Length of query: 194 Length of database: 38,378,431 Length adjustment: 101 Effective length of query: 93 Effective length of database: 26,729,697 Effective search space: 2485861821 Effective search space used: 2485861821 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 113 (48.1 bits)