BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0548.0026.p (287 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0707 [IQR] COG1028 Dehydrogenases with different specificities... 321 1e-87 CAC1331 [IQR] COG1028 Dehydrogenases with different specificitie... 303 2e-82 HI0048 [IQR] COG1028 Dehydrogenases with different specificities... 282 5e-76 BS_ycdF [IQR] COG1028 Dehydrogenases with different specificitie... 129 5e-30 idnO [IQR] COG1028 Dehydrogenases with different specificities (... 122 6e-28 >BH0707 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 284 Score = 321 bits (822), Expect = 1e-87, Method: Compositional matrix adjust. Identities = 154/284 (54%), Positives = 204/284 (71%), Gaps = 3/284 (1%) Query: 6 KLALQPALAGKVAVITGGAGVLGAVMAHELGRQGAKVAILNRTAEKGESLAAGINAAGGQ 65 +L+ Q L KVAV+TGG+GVLG L + GAKVA++ RT +K +++ I GG+ Sbjct: 2 ELSFQVDLTNKVAVVTGGSGVLGREFCLALAKCGAKVAVIGRTDDKLKAIVNEIIVNGGE 61 Query: 66 AVSVACDVTEDSSVHRAAAEVLNHWGPCDLLINGAGGNQPSAVTTKEIFTEGDLE--NPE 123 A++++ DV + + RA +V G CDLLINGAGGN P T + E DL + + Sbjct: 62 AIAISADVLAKTDMERAREQVRTELGTCDLLINGAGGNHPKGTTQLDYLREADLHGIDQD 121 Query: 124 ITSFFELSAAGFLQVMDLNLIGTLIPTQIFARQMLGRPGASVINISSMSAAAPMTKVPAY 183 + +FF+L A G V +LN +GTLIP Q+F++ MLG+ GAS++NISSM+A P+T++PAY Sbjct: 122 VRTFFDLDADGIRHVFELNFLGTLIPCQVFSQDMLGKEGASIVNISSMNAFTPLTRIPAY 181 Query: 184 SAAKAGVNNFTQWLAVHLAEAGIRVNAIAPGFFLTKQNEKLLLREDGELTERSHKIISQT 243 S AKA V+NFTQWLAVH ++ GIRVNAIAPGFFLT+QN LL+++DG T+R++KI+SQT Sbjct: 182 SGAKAAVSNFTQWLAVHFSKVGIRVNAIAPGFFLTEQNYSLLVKDDGTYTDRANKILSQT 241 Query: 244 PMRRFGKPEELLGTLLWLADESMSGFVTGITVPVDGGFMAYSGV 287 P RFGKPEEL+ TLLWL D S S FV G+ VPVDGGF AYSGV Sbjct: 242 PAERFGKPEELISTLLWLVDSSTS-FVNGVIVPVDGGFSAYSGV 284 >CAC1331 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 285 Score = 303 bits (775), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 149/284 (52%), Positives = 196/284 (69%), Gaps = 2/284 (0%) Query: 6 KLALQPALAGKVAVITGGAGVLGAVMAHELGRQGAKVAILNRTAEKGESLAAGINAAGGQ 65 KL L KVAV+TGG GVLG+ + L AKVAI++R E E AA I + G Sbjct: 2 KLPFNVNLKNKVAVVTGGTGVLGSSWVNALAECDAKVAIISRKLESAEKKAAEIVSNGKI 61 Query: 66 AVSVACDVTEDSSVHRAAAEVLNHWGPCDLLINGAGGNQPSAVTTKEIFTEGDL--ENPE 123 A+ + DV + +++ +A +L GPCD+LINGAGGN P+ TTKE DL +N + Sbjct: 62 AIGIEADVLDKAALKKAHEIILEKLGPCDILINGAGGNNPTGTTTKEYLYAEDLKEDNED 121 Query: 124 ITSFFELSAAGFLQVMDLNLIGTLIPTQIFARQMLGRPGASVINISSMSAAAPMTKVPAY 183 + +FF+L G V DLN +GTL+P+Q F++ M+ + G ++INISSM+A P+TK+PAY Sbjct: 122 LITFFDLEENGIKSVFDLNFLGTLLPSQEFSKDMIEKRGCTIINISSMNAFTPLTKIPAY 181 Query: 184 SAAKAGVNNFTQWLAVHLAEAGIRVNAIAPGFFLTKQNEKLLLREDGELTERSHKIISQT 243 S AKA V+NFT+WLAVHL++ IRVNAIAPGFFLTKQNE LL DG T RSHKI++ T Sbjct: 182 SGAKAAVSNFTKWLAVHLSKVQIRVNAIAPGFFLTKQNEALLKNPDGSFTGRSHKILNAT 241 Query: 244 PMRRFGKPEELLGTLLWLADESMSGFVTGITVPVDGGFMAYSGV 287 PM RFG+ +EL+G LL+L +E S FV G+ +PVDGGF +YSGV Sbjct: 242 PMGRFGEADELIGALLFLVNEEASSFVNGVVIPVDGGFSSYSGV 285 >HI0048 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 285 Score = 282 bits (721), Expect = 5e-76, Method: Compositional matrix adjust. Identities = 137/287 (47%), Positives = 194/287 (67%), Gaps = 2/287 (0%) Query: 1 VPFMAKLALQPALAGKVAVITGGAGVLGAVMAHELGRQGAKVAILNRTAEKGESLAAGIN 60 + F +A L K+ +ITG GVL + +A +L A +A+L+ E + +A IN Sbjct: 1 MEFTMNIAANHNLENKLIIITGAGGVLCSFLAKQLAYTKANIALLDLNFEAADKVAKEIN 60 Query: 61 AAGGQAVSVACDVTEDSSVHRAAAEVLNHWGPCDLLINGAGGNQPSAVTTKEIFTEGDLE 120 +GG+A + +V E ++ ++ +G CD+LINGAGGN P A T E F + DL Sbjct: 61 QSGGKAKAYKTNVLELENIKEVRNQIETDFGTCDILINGAGGNNPKATTDNE-FHQFDL- 118 Query: 121 NPEITSFFELSAAGFLQVMDLNLIGTLIPTQIFARQMLGRPGASVINISSMSAAAPMTKV 180 N +FF+L +G V +LN +G+L+PTQ+FA+ MLG+ GA++INISSM+A P+TK+ Sbjct: 119 NETTRTFFDLDKSGIEFVFNLNYLGSLLPTQVFAKDMLGKQGANIINISSMNAFTPLTKI 178 Query: 181 PAYSAAKAGVNNFTQWLAVHLAEAGIRVNAIAPGFFLTKQNEKLLLREDGELTERSHKII 240 PAYS AKA ++NFTQWLAV+ ++ GIR NAIAPGF ++ QN LL +G+ T+R++KI+ Sbjct: 179 PAYSGAKAAISNFTQWLAVYFSKVGIRCNAIAPGFLVSNQNLALLFDTEGKPTDRANKIL 238 Query: 241 SQTPMRRFGKPEELLGTLLWLADESMSGFVTGITVPVDGGFMAYSGV 287 + TPM RFG+ EELLG LL+L DE+ S FV G+ +PVDGGF AYSGV Sbjct: 239 TNTPMGRFGESEELLGALLFLIDENYSAFVNGVVLPVDGGFSAYSGV 285 >BS_ycdF [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 76 Score = 129 bits (324), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 0/275 (0%), Positives = 0/275 (0%), Gaps = 29/275 (10%) Query: 13 LAGKVAVITGGAGVLGAVMAHELGRQGAKVAILNRTAEKG--ESLAAGINAAGGQAVSVA 70 LA K+ G + R A + R + G + Sbjct: 5 LAKKLLQDITKRGSARVSSLSLVPRSENMAAAIRRVNSMQYDVYTQT-EVSNGKPSRRFR 63 Query: 71 CDVTEDSSVHRAAAEVLNHWGPCDLLINGAGGNQPSAVTTKEIFTEGDLENPEITSFFEL 130 Sbjct: 64 ------------------ 105 Query: 131 SAAGFLQVMDLNLIGTLIPTQIFARQMLGRP-GASVINISSMSAAAPMTKVPAYSAAKAG 189 Sbjct: 106 165 Query: 190 VNNFTQWLAVHLAEAGIRVNAIAPGFFLTKQNEKLLLREDGELTERSHKIISQTPMRRFG 249 Sbjct: 166 ------ 219 Query: 250 KPEELLGTLLWLADESMSGFVTGITVPVDGGFMAY 284 Sbjct: 220 - 253 >idnO [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 200 Score = 122 bits (307), Expect = 6e-28, Method: Compositional matrix adjust. Identities = 0/271 (0%), Positives = 0/271 (0%), Gaps = 25/271 (9%) Query: 12 ALAGKVAVITGGAGVLGAVMAHELGRQGAKVAILNRTAEKGESLAAGINAAGGQAVSVAC 71 + G + + + R + L + Sbjct: 6 GEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRAATIELGPLVRGLPHLRTIHRQ 65 Query: 72 DVTEDSSVHRAAAEVLNHWGPCDLLINGAGGNQPSAVTTKEIFTEGDLENPEITSFFELS 131 + EV + G + E Sbjct: 66 LYQDIFDWAGQLREVDIYQGDTPFCHFAYI------EKEGNALMQD----LE-------- 107 Query: 132 AAGFLQVMDLNLIGTLIPTQIFARQMLGRPGASVINISSMSAAAPMTKVPAYSAAKAGVN 191 V + + P ++ + Y + G+ Sbjct: 108 -EEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQLSWQGIE 166 Query: 192 NFTQWLAVHLAEAGIRVNAIAPGFFLTKQNEKLLLREDGELTERSHKIISQTPMRRFGKP 251 A G + + + Sbjct: 167 KEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE----- 221 Query: 252 EELLGTLLWLADESMSGFVTGITVPVDGGFM 282 Sbjct: 222 - 251 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.133 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,896,722 Number of extensions: 846388 Number of successful extensions: 4076 Number of sequences better than 1.0e-05: 313 Number of HSP's gapped: 3575 Number of HSP's successfully gapped: 313 Length of query: 287 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 182 Effective length of database: 26,268,361 Effective search space: 4780841702 Effective search space used: 4780841702 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)