BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0548.0025.p (155 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1154_2 [T] COG0642 Signal transduction histidine kinase 80 1e-15 DR0744 [T] COG0642 Signal transduction histidine kinase 79 2e-15 lin2727 [T] COG0642 Signal transduction histidine kinase 77 7e-15 lin2061 [T] COG0642 Signal transduction histidine kinase 75 4e-14 sll0798 [T] COG0642 Signal transduction histidine kinase 73 2e-13 >BH1154_2 [T] COG0642 Signal transduction histidine kinase Length = 274 Score = 80.1 bits (196), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 47/143 (32%), Positives = 80/143 (55%), Gaps = 4/143 (2%) Query: 3 EMLRQLLVEMEPLAKENQVQLEANLPTTPLLTEIDPDMIKRAIDNLLINALKFSLKPGVI 62 ++L+Q L E +++ +QL ++P PL + D I R DNL+ N LK+SL+ + Sbjct: 132 QLLQQALAEYGEALEQSSLQLRISVPEEPLYIKADGQKIWRVFDNLIQNILKYSLEHTRV 191 Query: 63 QVSLRLQKDTARICVENDGDPITEEQQRQLFERFYRAGHSQSLDHIPAGAGLGLSIARSI 122 +S++ +K+T I ++N + +L ERF R S++ + G+GLGL+IA+SI Sbjct: 192 YLSVKEKKETVEIIIKNVAKYELGDNVDELLERFKRGDQSRNTE----GSGLGLAIAKSI 247 Query: 123 ANLHHGELECEHSGGHFTFQLSL 145 +LH G L+ + G F + L Sbjct: 248 VDLHEGYLDIDVDGDLFKVIIQL 270 >DR0744 [T] COG0642 Signal transduction histidine kinase Length = 566 Score = 79.3 bits (194), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 55/142 (38%), Positives = 75/142 (52%), Gaps = 6/142 (4%) Query: 11 EMEPLAKENQVQLEANLPTTPLLTEIDPDMIKRAIDNLLINALKFSLKPGVIQVSLRLQK 70 E+ PLA +L + P P E DPD +++ I NL+ NALK K ++ S + + Sbjct: 422 ELAPLATGGS-ELRVSGPDIPF--EGDPDRLRQVIINLVGNALKAGAKTVTLESSSQEEG 478 Query: 71 DTARICVENDGDPITEEQQRQLFERFYRAGHSQSLDHIPAGAGLGLSIARSIANLHHGEL 130 R+ V +DG I E +LF+RFYR S+S D GAGLGLSIA SI + H G + Sbjct: 479 REVRLSVRDDGPGIPAEHLSRLFDRFYRVEDSRSRDQ--GGAGLGLSIAHSIVDAHGGRI 536 Query: 131 ECEHSGGHFTF-QLSLLQGAIP 151 E G T + LL G +P Sbjct: 537 WLESEVGRGTVAHVQLLVGDVP 558 >lin2727 [T] COG0642 Signal transduction histidine kinase Length = 182 Score = 77.4 bits (189), Expect = 7e-15, Method: Compositional matrix adjust. Identities = 0/148 (0%), Positives = 0/148 (0%), Gaps = 9/148 (6%) Query: 1 LGEMLRQLLVEMEPLAKENQVQLEANLPTTPLLTEIDPDMIKRAIDNLLINALKFSLKPG 60 Sbjct: 315 -- 372 Query: 61 VIQVSLRLQKDTARICVE--NDGDPITEEQQRQLFERFYRAGHSQSLDHIPAGAGLGLSI 118 Sbjct: 373 ---- 428 Query: 119 ARSIANLHHGELECE-HSGGHFTFQLSL 145 Sbjct: 429 456 >lin2061 [T] COG0642 Signal transduction histidine kinase Length = 182 Score = 75.1 bits (183), Expect = 4e-14, Method: Composition-based stats. Identities = 0/155 (0%), Positives = 0/155 (0%), Gaps = 9/155 (5%) Query: 1 LGEMLRQLLVEMEPLAKENQVQLEANLPTTPLLTEIDPDMIKRAIDNLLINALKFSLKPG 60 Sbjct: 443 502 Query: 61 VI-QVSLRLQKDTAR----ICVENDGDPITEEQQRQLFERFYRAGHSQSLDHIPAGAGLG 115 Sbjct: 503 - 561 Query: 116 LSIARSIANLHHGELECEH---SGGHFTFQLSLLQ 147 Sbjct: 562 596 >sll0798 [T] COG0642 Signal transduction histidine kinase Length = 454 Score = 72.8 bits (177), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 45/146 (30%), Positives = 73/146 (50%), Gaps = 5/146 (3%) Query: 1 LGEMLRQLLVEMEPLAKENQVQLEANLPTTPLLTEIDPDMIKRAIDNLLINALKFSLKPG 60 L +++ L E LA + L ++L + + + + R + NL+ NA++++ G Sbjct: 301 LNDLVSDLTEEFLELAIAAHIDLSSDLSSGEVYAWGNESQLYRLVSNLIANAIQYTTAGG 360 Query: 61 VIQVSLRLQKDTARICVENDGDPITEEQQRQLFERFYRAGHSQSLDHIPAGAGLGLSIAR 120 + ++L + A I V++ G I +QQ +FERFYR +S G GLGL+IA+ Sbjct: 361 RVDITLTSHEQMAIITVQDTGIGIAPDQQEHIFERFYRVNRDRSRK--TGGTGLGLAIAQ 418 Query: 121 SIANLHHGELECEHS---GGHFTFQL 143 I H G L E + G FT QL Sbjct: 419 VITVKHRGSLTVESALGKGSLFTIQL 444 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.137 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 10,630,605 Number of extensions: 365261 Number of successful extensions: 1373 Number of sequences better than 1.0e-05: 150 Number of HSP's gapped: 1297 Number of HSP's successfully gapped: 150 Length of query: 155 Length of database: 38,378,431 Length adjustment: 97 Effective length of query: 58 Effective length of database: 27,191,033 Effective search space: 1577079914 Effective search space used: 1577079914 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 111 (47.4 bits)