BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0548.0022.p (221 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC1489 [I] COG0671 Membrane-associated phospholipid phosphatase 70 3e-12 >CAC1489 [I] COG0671 Membrane-associated phospholipid phosphatase Length = 346 Score = 70.1 bits (170), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 5/104 (4%), Positives = 15/104 (14%) Query: 95 GLVACYMIYLQFQTTVPRPQLSSSGVLIHLVEWVYSHDQPFNCFPSIHVFTSYLMIKSVQ 154 + + S L +Y P + Sbjct: 95 NDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPA 154 Query: 155 GHAAFSRTFKAAVYFCAWLIICSTLFVKQHVLLDAAGAIALAEI 198 + A + ++ ++ Sbjct: 155 VSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDL 198 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.330 0.139 0.439 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 13,166,393 Number of extensions: 456924 Number of successful extensions: 615 Number of sequences better than 1.0e-05: 1 Number of HSP's gapped: 618 Number of HSP's successfully gapped: 1 Length of query: 221 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 119 Effective length of database: 26,614,363 Effective search space: 3167109197 Effective search space used: 3167109197 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 114 (48.5 bits)