BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0547.0048.p (440 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yqjI [G] COG0362 6-phosphogluconate dehydrogenase 646 0.0 BS_gntZ [G] COG0362 6-phosphogluconate dehydrogenase 514 e-145 YGR256w [G] COG0362 6-phosphogluconate dehydrogenase 505 e-143 YHR183w [G] COG0362 6-phosphogluconate dehydrogenase 489 e-138 STM0438 [R] COG0790 FOG: TPR repeat, SEL1 subfamily 434 e-121 >BS_yqjI [G] COG0362 6-phosphogluconate dehydrogenase Length = 207 Score = 646 bits (1667), Expect = 0.0, Method: Compositional matrix adjust. Identities = 0/405 (0%), Positives = 0/405 (0%) Query: 34 LEVPRKILIMVQAGKATDATIEQLLPHLDQGDIIIDGGNAYFPDTVRRNKELEAKGFRFI 93 + + + + + + + + F Sbjct: 1 MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCGGRHGRGGGRRQRFFGH 60 Query: 94 GTGVSGGEEGALKGPSIMPGGQESAYKLVEPILTAISAKVNGEPCCTYIGPDGAGHYVKM 153 G + + S ++ L + T + + Sbjct: 61 GELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYPTLDFLQEQSLITIREEEG 120 Query: 154 VHNGIEYGDMQLIGEAYHLLKDVLGLDAKELHSIFADWNSGELDSYLIEITKDIFAEYDE 213 I + + + + + + + A ++ K + Sbjct: 121 GKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQNPQMKRALDNFKAVLDLRVN 180 Query: 214 ETGKPMVDVILDAAGQKGTGKWTSQSSLDLGVPLSMITESVFSRFLSAMKEERVAASKVL 273 Sbjct: 181 240 Query: 274 SGPVTEPFQGDKAEFIENVRKALFASKIVSYAQGFAQLRVASEEYNWDLKYGNLAKIWRG 333 Sbjct: 241 300 Query: 334 GCIIRSRFLQNITDAYENNPELKNLLLDPFFKNIMDTYQSAWRQVVSAAVAQGVPVPGFS 393 Sbjct: 301 360 Query: 394 SALAYYDSYRTERLPANLLQAQRDYFGAHTFKRVDKEGVFHHNWL 438 Sbjct: 361 405 >BS_gntZ [G] COG0362 6-phosphogluconate dehydrogenase Length = 468 Score = 514 bits (1323), Expect = e-145, Method: Compositional matrix adjust. Identities = 238/437 (54%), Positives = 320/437 (73%) Query: 1 VYNRSPEKTHDLINEAEGKNLTGTFSIEEFVESLEVPRKILIMVQAGKATDATIEQLLPH 60 VYN + + T LI + +G++L+ + +E+FV+SLE PRKI +MV AGK D+ I+ L P Sbjct: 30 VYNYTRDLTDQLIQKLDGQSLSPYYELEDFVQSLEKPRKIFLMVTAGKPVDSVIQSLKPL 89 Query: 61 LDQGDIIIDGGNAYFPDTVRRNKELEAKGFRFIGTGVSGGEEGALKGPSIMPGGQESAYK 120 L++GD+I+DGGN+++ DT RR EL+ KG ++G G+SGGE GAL GPSIMPGG Y+ Sbjct: 90 LEEGDVIMDGGNSHYEDTERRYDELKEKGIGYLGVGISGGEVGALTGPSIMPGGDRDVYE 149 Query: 121 LVEPILTAISAKVNGEPCCTYIGPDGAGHYVKMVHNGIEYGDMQLIGEAYHLLKDVLGLD 180 PILT I+A+V +PCC YIGP GAGH+ KMVHNGIEY DMQLI EAY L++ L L Sbjct: 150 KAAPILTKIAAQVGDDPCCVYIGPKGAGHFTKMVHNGIEYADMQLIAEAYTFLRETLRLP 209 Query: 181 AKELHSIFADWNSGELDSYLIEITKDIFAEYDEETGKPMVDVILDAAGQKGTGKWTSQSS 240 E+ SIF WN GEL SYLIEIT +I + DE+TG+P++DVILD GQKGTGKWTS + Sbjct: 210 LDEIASIFETWNQGELKSYLIEITAEILRKKDEKTGQPLIDVILDKTGQKGTGKWTSMQA 269 Query: 241 LDLGVPLSMITESVFSRFLSAMKEERVAASKVLSGPVTEPFQGDKAEFIENVRKALFASK 300 +D G+P ++ITES+F+R+LS++KEER+AA VL+GP E DK +IE VR+AL+ K Sbjct: 270 IDNGIPSTIITESLFARYLSSLKEERMAAQDVLAGPEAEEKHLDKDTWIEYVRQALYMGK 329 Query: 301 IVSYAQGFAQLRVASEEYNWDLKYGNLAKIWRGGCIIRSRFLQNITDAYENNPELKNLLL 360 + +YAQGFAQ +++SE Y W+L ++A I+RGGCIIR+ FL I++A+ P L NLL+ Sbjct: 330 VCAYAQGFAQYKMSSELYGWNLPLKDIALIFRGGCIIRADFLNVISEAFSEQPNLANLLI 389 Query: 361 DPFFKNIMDTYQSAWRQVVSAAVAQGVPVPGFSSALAYYDSYRTERLPANLLQAQRDYFG 420 P+F + + YQ+ R+VV ++ G+ P ++AL+YYD YRT R ANLLQAQRDYFG Sbjct: 390 APYFTDKLHAYQTGLRKVVCEGISTGISFPCLTTALSYYDGYRTGRSNANLLQAQRDYFG 449 Query: 421 AHTFKRVDKEGVFHHNW 437 AHT++R D +GVFH NW Sbjct: 450 AHTYERTDMDGVFHTNW 466 >YGR256w [G] COG0362 6-phosphogluconate dehydrogenase Length = 492 Score = 505 bits (1300), Expect = e-143, Method: Compositional matrix adjust. Identities = 242/448 (54%), Positives = 329/448 (73%), Gaps = 15/448 (3%) Query: 2 YNRSPEKTHD-LINEAEGKNLTGTFSIEEFVESLEVPRKILIMVQAGKATDATIEQLLPH 60 YNR+ K L NEA+GK++ G SIE+ V L+ PRKI+++++AG D I++L+PH Sbjct: 34 YNRTQSKVDRFLANEAKGKSIIGATSIEDLVAKLKKPRKIMLLIKAGAPVDTLIKELVPH 93 Query: 61 LDQGDIIIDGGNAYFPDTVRRNKELEAKGFRFIGTGVSGGEEGALKGPSIMPGGQESAYK 120 LD+GDIIIDGGN++FPDT RR +EL +G F+G+GVSGGE+GA GPS+MPGG A+ Sbjct: 94 LDKGDIIIDGGNSHFPDTNRRYEELTKQGILFVGSGVSGGEDGARFGPSLMPGGSAEAWP 153 Query: 121 LVEPILTAISAKVNGEPCCTYIGPDGAGHYVKMVHNGIEYGDMQLIGEAYHLLKDVLGLD 180 ++ I +I+AK NGEPCC ++GP G+GHYVKMVHNGIEYGDMQLI EAY ++K + Sbjct: 154 HIKNIFQSIAAKSNGEPCCEWVGPAGSGHYVKMVHNGIEYGDMQLICEAYDIMKRIGRFT 213 Query: 181 AKELHSIFADWNSGELDSYLIEITKDIFAEYDEETGKPMVDVILDAAGQKGTGKWTSQSS 240 KE+ +F WN+G LDS+LIEIT+DI ++D+ GKP+V+ I+D AGQKGTGKWT+ ++ Sbjct: 214 DKEISEVFDKWNTGVLDSFLIEITRDIL-KFDDVDGKPLVEKIMDTAGQKGTGKWTAINA 272 Query: 241 LDLGVPLSMITESVFSRFLSAMKEERVAASKVLSGP-VTEPFQGDKAEFIENVRKALFAS 299 LDLG+P+++I E+VF+R LSA+K+ER ASK+L+GP V + D+ +F+ ++ +AL+AS Sbjct: 273 LDLGMPVTLIGEAVFARCLSAIKDERKRASKLLAGPTVPKDAIHDREQFVYDLEQALYAS 332 Query: 300 KIVSYAQGFAQLRVASEEYNWDLKYGNLAKIWRGGCIIRSRFLQNITDAYENNPELKNLL 359 KI+SYAQGF +R A+ Y W L +A +WRGGCIIRS FL IT AY ++P+L+NLL Sbjct: 333 KIISYAQGFMLIREAARSYGWKLNNPAIALMWRGGCIIRSVFLAEITKAYRDDPDLENLL 392 Query: 360 LDPFFKNIMDTYQSAWRQVVSAAVAQGVPVPGFSSALAYYDSYRTERLPANLLQAQRDYF 419 + FF + + QS WR+ ++ A G+P P FS+ALA+YD YR+ERLPANLLQAQRDYF Sbjct: 393 FNEFFASAVTKAQSGWRRTIALAATYGIPTPAFSTALAFYDGYRSERLPANLLQAQRDYF 452 Query: 420 GAHTFK----------RVDKEGVFHHNW 437 GAHTF+ VDK+ H NW Sbjct: 453 GAHTFRILPECASAHLPVDKD--IHINW 478 >YHR183w [G] COG0362 6-phosphogluconate dehydrogenase Length = 489 Score = 489 bits (1260), Expect = e-138, Method: Compositional matrix adjust. Identities = 246/448 (54%), Positives = 332/448 (74%), Gaps = 15/448 (3%) Query: 2 YNRSPEKT-HDLINEAEGKNLTGTFSIEEFVESLEVPRKILIMVQAGKATDATIEQLLPH 60 YNR+ K H L NEA+GK++ G SIE+F+ L+ PRK++++V+AG DA I Q++P Sbjct: 31 YNRTQSKVDHFLANEAKGKSIIGATSIEDFISKLKRPRKVMLLVKAGAPVDALINQIVPL 90 Query: 61 LDQGDIIIDGGNAYFPDTVRRNKELEAKGFRFIGTGVSGGEEGALKGPSIMPGGQESAYK 120 L++GDIIIDGGN++FPD+ RR +EL+ KG F+G+GVSGGEEGA GPS+MPGG E A+ Sbjct: 91 LEKGDIIIDGGNSHFPDSNRRYEELKKKGILFVGSGVSGGEEGARYGPSLMPGGSEEAWP 150 Query: 121 LVEPILTAISAKVNGEPCCTYIGPDGAGHYVKMVHNGIEYGDMQLIGEAYHLLKDVLGLD 180 ++ I +ISAK +GEPCC ++GP GAGHYVKMVHNGIEYGDMQLI EAY ++K + G Sbjct: 151 HIKNIFQSISAKSDGEPCCEWVGPAGAGHYVKMVHNGIEYGDMQLICEAYDIMKRLGGFT 210 Query: 181 AKELHSIFADWNSGELDSYLIEITKDIFAEYDEETGKPMVDVILDAAGQKGTGKWTSQSS 240 KE+ +FA WN+G LDS+L+EIT+DI ++D+ GKP+V+ I+D AGQKGTGKWT+ ++ Sbjct: 211 DKEISDVFAKWNNGVLDSFLVEITRDIL-KFDDVDGKPLVEKIMDTAGQKGTGKWTAINA 269 Query: 241 LDLGVPLSMITESVFSRFLSAMKEERVAASKVLSGP-VTEPFQGDKAEFIENVRKALFAS 299 LDLG+P+++I E+VF+R LSA+K ER+ ASKVL GP V + D+ +F++++ +AL+AS Sbjct: 270 LDLGMPVTLIGEAVFARCLSALKNERIRASKVLPGPEVPKDAVKDREQFVDDLEQALYAS 329 Query: 300 KIVSYAQGFAQLRVASEEYNWDLKYGNLAKIWRGGCIIRSRFLQNITDAYENNPELKNLL 359 KI+SYAQGF +R A+ Y W L +A +WRGGCIIRS FL IT AY P+L+NLL Sbjct: 330 KIISYAQGFMLIREAAATYGWKLNNPAIALMWRGGCIIRSVFLGQITKAYREEPDLENLL 389 Query: 360 LDPFFKNIMDTYQSAWRQVVSAAVAQGVPVPGFSSALAYYDSYRTERLPANLLQAQRDYF 419 + FF + + QS WR+ ++ A G+P P FS+AL++YD YR+ERLPANLLQAQRDYF Sbjct: 390 FNKFFADAVTKAQSGWRKSIALATTYGIPTPAFSTALSFYDGYRSERLPANLLQAQRDYF 449 Query: 420 GAHTFK----------RVDKEGVFHHNW 437 GAHTF+ VDK+ H NW Sbjct: 450 GAHTFRVLPECASDNLPVDKD--IHINW 475 >STM0438 [R] COG0790 FOG: TPR repeat, SEL1 subfamily Length = 673 Score = 434 bits (1115), Expect = e-121, Method: Compositional matrix adjust. Identities = 29/438 (6%), Positives = 85/438 (19%), Gaps = 5/438 (1%) Query: 1 VYNRSPEKTHDLI-NEAEGKNLTGTFSIEEFVESLEVPRKILIMVQAGKATDATIEQLLP 59 + TF+ + E E + L +A +T L Sbjct: 30 RVITNKIAHLIRGCGYQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGL 89 Query: 60 HLDQGDIIIDGGNAYFPDTVRRNKELEAKGFRFIGTGVSGGEEGALKGPSIMPGGQESAY 119 + + + G A F ++ K L Sbjct: 90 DIIKREYAALGMKANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTP 149 Query: 120 KLVEPILTAISAKVNGEPCCTYIGPDGAGHYVKMVHNGIEYGDMQLIGEAYHLLKDVLGL 179 ++ Y A + + + + E Sbjct: 150 SQAAASAIGERDRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQANEEVRKRWQNKIR 209 Query: 180 DAKELHSIFADWNSGELDSYLIEITKDIFAEYDEETGKPMVDVILDAAGQKGTGKWTSQS 239 + + EL L+ D++ + + + Sbjct: 210 YLLVDEYQDTNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALK 269 Query: 240 SLDLGVPLSMITESVFSRFLSAMKEERVAASKVLSGPVTEPFQGDKAEFIENVRKALFAS 299 + L + + + V + L + + N Sbjct: 270 VIKLEQNYRSSGRILKAANILIANNPHVFEKR-LFSELGYGAELKV-LSANNEEHEAERV 327 Query: 300 KIVSYAQGFAQLRVASEEYNWDLKYGNLAKIWRGGCIIRSRFLQNITDAYENNPELKNLL 359 A F + + R + + ++ + + Sbjct: 328 TGELIAHHFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFS--RPEIKD 385 Query: 360 LDPFFKNIMDTYQSAWRQVVSAAVAQGVPVPGFSSALAYYDSYRTERLPANLLQAQRDYF 419 L + + + + + + + + + + A+ Sbjct: 386 LLAYLRVLTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTL 445 Query: 420 GAHTFKRVDKEGVFHHNW 437 ++ + + + Sbjct: 446 SGRGYEALTRFTHWLAEI 463 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.136 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 34,387,980 Number of extensions: 1535210 Number of successful extensions: 3193 Number of sequences better than 1.0e-05: 16 Number of HSP's gapped: 3198 Number of HSP's successfully gapped: 17 Length of query: 440 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 331 Effective length of database: 25,807,025 Effective search space: 8542125275 Effective search space used: 8542125275 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 117 (49.7 bits)