BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0547.0033.p (281 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC1353_1 [G] COG1263 Phosphotransferase system IIC components, ... 102 6e-22 CC0538_1 [G] COG1263 Phosphotransferase system IIC components, g... 96 6e-20 BH0673_1 [G] COG1263 Phosphotransferase system IIC components, g... 96 6e-20 BH0422_1 [G] COG1263 Phosphotransferase system IIC components, g... 95 1e-19 STM0685_1 [G] COG1263 Phosphotransferase system IIC components, ... 93 5e-19 >CAC1353_1 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 409 Score = 102 bits (254), Expect = 6e-22, Method: Compositional matrix adjust. Identities = 76/267 (28%), Positives = 118/267 (44%), Gaps = 18/267 (6%) Query: 1 LGRAIMLPTMVLPAAAILLSLGS--LPWSAWGLSSVSEVTTYAGQGIFYFMPYLFAVGVA 58 LG+++M P VLPAA +LL LG L +W ++ AG GIF + +FA+G+A Sbjct: 14 LGKSLMTPIAVLPAAGLLLRLGQPDLLNISWMMA--------AGNGIFNNLAMIFAIGIA 65 Query: 59 WGLSNQXXXXXXXXXXXXFTYDRIVSNMGDGAVQPATXXXXXXXXXXXXXHNRFKNIKLP 118 G + + + V+ + + +N++K+ +LP Sbjct: 66 VGFAEENNGVAGLSAAVGYFVLTNVATSFNKHIDMGVLGGIIVGILAGNLYNKYKSTRLP 125 Query: 119 EAIQFFGGSRFVLLFMGLFSALFAWVMLGLSPVLQHGLDILFRDIQATXXXXXXXXXXXX 178 + + FFGG R V + L S + + + P +Q+ ++ + Sbjct: 126 DFLGFFGGRRLVPILTSLCSLVLGLISGLVWPAIQNVINAFGNSVSHAGVVGSFIYGLLN 185 Query: 179 XXLTAFGLHHILNNVFWFQLGTFTTPDGSAVVQGDLPRFFAGDPTAGIFMAGLXXXXPDH 238 L GLHH+LN +FWFQ GTF + G +V GDL RFFA D TAG +M G P Sbjct: 186 RLLIPIGLHHVLNTLFWFQFGTFKSASGK-IVTGDLHRFFALDKTAGTYMTGF---FPIM 241 Query: 239 DVCAAGDCLCDYSGSAGGFKAEDQEDV 265 CL S + K E+++ V Sbjct: 242 MFALPAACLAMISAA----KKENRKKV 264 >CC0538_1 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 435 Score = 95.9 bits (237), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 85/264 (32%), Positives = 112/264 (42%), Gaps = 40/264 (15%) Query: 1 LGRAIMLPTMVLPAAAILLSLGS--------LPWSAWGLS-SVSEVTTYAGQGIFYFMPY 51 LGRA+MLP VLP AA+LL +G L + G+S SV+ AG IF + Sbjct: 11 LGRALMLPIAVLPVAALLLRIGQPDLLGAPALAAATHGMSLSVANTFGAAGGAIFASLGL 70 Query: 52 LFAVGVAWGLSNQXXXXXXXXXXXXFTY------------DRIVSNMGDGAVQPATXX-- 97 +FA+GVA GL+ + + + + +GA A Sbjct: 71 IFAIGVAVGLARENHGAAGLAGVVCYVIATKGVEALLVAPPEVAAKAVEGAKDLAIAAWK 130 Query: 98 -----------XXXXXXXXXXXHNRFKNIKLPEAIQFFGGSRFVLLFMGLFSALFAWVML 146 +NR+ I+LPE + FFGG RFV + GL + A + Sbjct: 131 AKEIGKLSIPVGILSGVISGALYNRYSTIQLPEYLAFFGGRRFVPIVAGLAGVVLALLFG 190 Query: 147 GLSPVLQHGLDILFRDIQATXXXXXXXXXXXXXXLTAFGLHHILNNVFWFQLGTFTTPDG 206 L+ G+D L + A+ L GLHHILNNV WF LG F Sbjct: 191 AFWSTLEAGVDGLSGLVTASGDLGLVVYGLLNRLLIVTGLHHILNNVVWFILGDFNG--- 247 Query: 207 SAVVQGDLPRFFAGDPTAGIFMAG 230 V GDL RF AGD TAG FM+G Sbjct: 248 ---VTGDLNRFAAGDKTAGAFMSG 268 >BH0673_1 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 390 Score = 95.9 bits (237), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 76/235 (32%), Positives = 103/235 (43%), Gaps = 22/235 (9%) Query: 1 LGRAIMLPTMVLPAAAILLSLG-----SLPWSAWGLSSVSEVTTYAGQGIFYFMPYLFAV 55 +GR+++LP LP A +LL LG ++P+ A AG +F + LFA+ Sbjct: 8 IGRSLLLPIAALPVAGLLLRLGQEDLLNIPFMA-----------AAGDALFSNLALLFAI 56 Query: 56 GVAWGLSNQXXXXXXXXXXXXFTYDRIVSNMGDGAVQPATXXXXXXXXXXXXXHNRFKNI 115 GVA G + F + + + +NRFK Sbjct: 57 GVAVGFAKDSHGAAALAGAIGFFVLTEGTQAINETINMGVLGGILSGVVAGMTYNRFKET 116 Query: 116 KLPEAIQFFGGSRFVLLFMGLFSALFAWVMLGLSPVLQHGLDILFRDIQATXXXXXXXXX 175 KLPE + FFGG RFV + GLF + A + P +Q LD + I Sbjct: 117 KLPEWLAFFGGRRFVPIMTGLFMLVLAIFFGFVWPTVQGWLDSFGQWIIGAGATGVGVYG 176 Query: 176 XXXXXLTAFGLHHILNNVFWFQLGTFTTPDGSAVVQGDLPRFFAGDPTAGIFMAG 230 L GLHH++N + WF G F +G+ GDL RFFAGDP AGIFMAG Sbjct: 177 FFNRLLIPLGLHHVINTLVWFVFGEF---NGAT---GDLHRFFAGDPNAGIFMAG 225 >BH0422_1 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 375 Score = 95.1 bits (235), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 79/234 (33%), Positives = 109/234 (46%), Gaps = 20/234 (8%) Query: 1 LGRAIMLPTMVLPAAAILLSLGSLPWSAWGLSSVSEVTTYAGQGIFYFMPYLFAVGVAWG 60 +G+++MLP VLPAAAILL LG AG +F +P +FA+GVA G Sbjct: 8 IGKSLMLPIAVLPAAAILLRLGQEDLFN------QAFIAAAGAAVFDHLPLIFAIGVAIG 61 Query: 61 LSNQXXXXXXXXXXXXFTYDRIVSNMGDGAVQPATXXXXXXXXXXXXXHNRFKNIKLPEA 120 S + + D V A +NRF ++KLP+ Sbjct: 62 FSKDSNGTAGLAGAIGYLVLVNGTQAIDPNVDMAILGGIISGIVAGLLYNRFHDVKLPQW 121 Query: 121 IQFFGGSRFVLLF----MGLFSALFAWVMLGLSPVLQHGLDILFRDIQATXXXXXXXXXX 176 + FFGG RFV + M +F+ LF +V P +Q G++ + + I Sbjct: 122 LGFFGGRRFVPIITATVMVIFAGLFGYVW----PPIQDGINQIGQWIIGGGALGVGIFGF 177 Query: 177 XXXXLTAFGLHHILNNVFWFQLGTFTTPDGSAVVQGDLPRFFAGDPTAGIFMAG 230 L GLHH+LN++ WF G + +G+ GDL RFFAGDPTAG FMAG Sbjct: 178 LNRLLIPVGLHHVLNSLVWFVFGEY---EGAT---GDLHRFFAGDPTAGSFMAG 225 >STM0685_1 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 388 Score = 92.8 bits (229), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 74/235 (31%), Positives = 104/235 (44%), Gaps = 17/235 (7%) Query: 1 LGRAIMLPTMVLPAAAILLSLG-----SLPWSAWGLSSVSEVTTYAGQGIFYFMPYLFAV 55 LGRA+ LP VLP AA+LL G ++P+ A AG IF + +FA+ Sbjct: 10 LGRALQLPIAVLPVAALLLRFGQPDLLNMPFIA-----------QAGGSIFDNLALVFAI 58 Query: 56 GVAWGLSNQXXXXXXXXXXXXFTYDRIVSNMGDGAVQPATXXXXXXXXXXXXXHNRFKNI 115 GVA S + + + +NR+ I Sbjct: 59 GVASSWSKDSAGAAALAGAVGYFVMTKAMVTINPEINMGVLAGIITGLVGGAVYNRWSGI 118 Query: 116 KLPEAIQFFGGSRFVLLFMGLFSALFAWVMLGLSPVLQHGLDILFRDIQATXXXXXXXXX 175 KLP+ + FFGG RFV + G F + A + + P +QHG+ I + Sbjct: 119 KLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHGIHAGGEWIVSAGALGSGIFG 178 Query: 176 XXXXXLTAFGLHHILNNVFWFQLGTFTTPDGSAVVQGDLPRFFAGDPTAGIFMAG 230 L GLH +LN + WFQ+G FT G+ V GD+ RF+AGD TAG+FM+G Sbjct: 179 FINRLLIPTGLHQVLNTIAWFQIGEFTNAAGT-VFHGDINRFYAGDGTAGMFMSG 232 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.326 0.143 0.456 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,116,258 Number of extensions: 758336 Number of successful extensions: 1494 Number of sequences better than 1.0e-05: 37 Number of HSP's gapped: 1433 Number of HSP's successfully gapped: 47 Length of query: 281 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 176 Effective length of database: 26,268,361 Effective search space: 4623231536 Effective search space used: 4623231536 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 115 (48.9 bits)