BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0547.0027.p (175 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2358 [R] COG0693 Putative intracellular protease/amidase 245 2e-65 MA2531 [R] COG0693 Putative intracellular protease/amidase 129 3e-30 CAC0991 [R] COG0693 Putative intracellular protease/amidase 115 3e-26 PA0355 [R] COG0693 Putative intracellular protease/amidase 114 7e-26 DR1199 [R] COG0693 Putative intracellular protease/amidase 111 7e-25 >lin2358 [R] COG0693 Putative intracellular protease/amidase Length = 64 Score = 245 bits (626), Expect = 2e-65, Method: Compositional matrix adjust. Identities = 0/172 (0%), Positives = 0/172 (0%), Gaps = 1/172 (0%) Query: 1 MRLTGKKVIALVDDEFEDLELWYPVYRVREEGAEVHLAGLEKDKTYVGKYGVPAKAEYSW 60 MR K ++ YR + G E +K + ++ + Sbjct: 1 MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNG 60 Query: 61 DELDAADYDGILVPGGWAPDKIRRXXXAVLKLVQDFNAAKKPIGQICHAGWVLISAKILE 120 Sbjct: 61 - 119 Query: 121 GVTVTSTPGIRDDMENAGAIWKDEAVVTDGHIISARRPPDLPPYGKAFCDAL 172 Sbjct: 120 171 >MA2531 [R] COG0693 Putative intracellular protease/amidase Length = 166 Score = 129 bits (323), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 66/166 (39%), Positives = 104/166 (62%), Gaps = 2/166 (1%) Query: 7 KVIALVDDEFEDLELWYPVYRVREEGAEVHLAGLEKDKTYVGKYGVPAKAEYSWDELDAA 66 K + D FEDLEL+YP +R++EEG H+A ++K GK+G A+ ++ ++++A Sbjct: 2 KALVFGADGFEDLELFYPYHRLKEEGITTHVASMKKGPI-TGKHGYEITADIAFKDVNSA 60 Query: 67 DYDGILVPGGWAPDKIRRXXXAVLKLVQDFNAAKKPIGQICHAGWVLISAKILEGVTVTS 126 DYD I++ GG P+K+R A L++V++F KP+ ICH VL+SA +++G T Sbjct: 61 DYDIIVISGGKGPEKMRLDKDA-LEIVKNFFKKNKPVASICHGPQVLVSADVIKGRKATC 119 Query: 127 TPGIRDDMENAGAIWKDEAVVTDGHIISARRPPDLPPYGKAFCDAL 172 GIRDD+ AGA ++D VV DG+++S+R P DL +G+ L Sbjct: 120 WIGIRDDIIAAGAQYEDSEVVVDGNLVSSRNPGDLHAFGREMIKLL 165 >CAC0991 [R] COG0693 Putative intracellular protease/amidase Length = 169 Score = 115 bits (289), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 61/158 (38%), Positives = 90/158 (56%), Gaps = 1/158 (0%) Query: 7 KVIALVDDEFEDLELWYPVYRVREEGAEVHLAGLEKDKTYVGKYGVPAKAEYSWDELDAA 66 +I LV+D FED+EL YP+YR+RE G +V L G Y G +G + S +E+D Sbjct: 3 NLIILVEDGFEDIELLYPLYRLREAGYDVTLVGTGSKYNYTGTHGYIVDVDASAEEIDEN 62 Query: 67 DYDGILVPGGWAPDKIRRXXXAVLKLVQDFNAAKKPIGQICHAGWVLISAKILEGVTVTS 126 +YDG+++PGG A K+R V ++++ K + IC +VLISAK+L G VT Sbjct: 63 NYDGVIIPGGSASYKLRTNDD-VKRIIRHMKEKDKLLAVICDGNYVLISAKVLSGHKVTC 121 Query: 127 TPGIRDDMENAGAIWKDEAVVTDGHIISARRPPDLPPY 164 T I DD+ NAG + D +II+A+ +LP + Sbjct: 122 TEAISDDVINAGGEYVRIGNCVDKNIITAKMQVNLPQF 159 >PA0355 [R] COG0693 Putative intracellular protease/amidase Length = 179 Score = 114 bits (286), Expect = 7e-26, Method: Compositional matrix adjust. Identities = 66/176 (37%), Positives = 101/176 (57%), Gaps = 5/176 (2%) Query: 3 LTGKKVIALVDDEFEDLELWYPVYRVREEGAEVHLAGLEKDKTYVGKYGVPAKA---EYS 59 L GK V ALV D FE +EL P + + GA V + + + + PA+A + + Sbjct: 5 LHGKVVAALVTDGFEQVELTGPKKALEDAGATVRILSDKAGEVRGWNHHQPAEAFRVDGT 64 Query: 60 WDELDAADYDGILVPGG-WAPDKIRRXXXAVLKLVQDFNAAKKPIGQICHAGWVLISAKI 118 +++ DYD +L+PGG D+IR A +L A KP+ ICH W+LISA + Sbjct: 65 FEDASLDDYDALLLPGGVINSDQIRSLAKA-QELAIRAEQASKPVAVICHGAWLLISAGL 123 Query: 119 LEGVTVTSTPGIRDDMENAGAIWKDEAVVTDGHIISARRPPDLPPYGKAFCDALAG 174 ++G T+TS P ++DD+ NAG W D+ V DG ++S+R+P D+P + + F + LAG Sbjct: 124 VQGRTLTSWPSLKDDINNAGGHWVDQEVAVDGKLVSSRKPEDIPAFNRRFIEILAG 179 >DR1199 [R] COG0693 Putative intracellular protease/amidase Length = 190 Score = 111 bits (277), Expect = 7e-25, Method: Compositional matrix adjust. Identities = 65/176 (36%), Positives = 94/176 (53%), Gaps = 6/176 (3%) Query: 3 LTGKKVIALVDDEFEDLELWYPVYRVREEGAEVHLAGLEKDKTYVGKYGVPAKAEYSWD- 61 LTGKK+ L D E++EL P + G L LE + K + + +Y D Sbjct: 7 LTGKKIAILAADGVEEIELTSPRAAIEAAGGTTELISLEPGEIQSMKGDIEPQEKYRVDH 66 Query: 62 ---ELDAADYDGILVPGGWA-PDKIRRXXXAVLKLVQDFNAAKKPIGQICHAGWVLISAK 117 E+ +DYDG+L+PGG PDK+R A +K V+D A KPI ICH W L Sbjct: 67 VVSEVQVSDYDGLLLPGGTVNPDKLRLEEGA-MKFVRDMYDAGKPIAAICHGPWSLSETG 125 Query: 118 ILEGVTVTSTPGIRDDMENAGAIWKDEAVVTDGHIISARRPPDLPPYGKAFCDALA 173 I +G+ +TS P ++ ++ AGA W DE VTD ++++R+P DLP + K + A Sbjct: 126 IAQGLKMTSWPSLKRELTLAGAQWVDEECVTDKGVVTSRKPDDLPAFNKKIVEEFA 181 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.139 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 14,299,051 Number of extensions: 556213 Number of successful extensions: 1616 Number of sequences better than 1.0e-05: 28 Number of HSP's gapped: 1575 Number of HSP's successfully gapped: 28 Length of query: 175 Length of database: 38,378,431 Length adjustment: 99 Effective length of query: 76 Effective length of database: 26,960,365 Effective search space: 2048987740 Effective search space used: 2048987740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 112 (47.8 bits)