BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0547.0024.p (231 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3238 [F] COG0775 Nucleoside phosphorylase 218 6e-57 BH1279 [F] COG0775 Nucleoside phosphorylase 169 5e-42 DR1451 [F] COG0775 Nucleoside phosphorylase 167 9e-42 YPO3384 [F] COG0775 Nucleoside phosphorylase 150 1e-36 STM0207 [F] COG0775 Nucleoside phosphorylase 146 3e-35 >BH3238 [F] COG0775 Nucleoside phosphorylase Length = 233 Score = 218 bits (555), Expect = 6e-57, Method: Compositional matrix adjust. Identities = 120/229 (52%), Positives = 154/229 (67%), Gaps = 2/229 (0%) Query: 5 IGLIGAMDEEIKLLLESMEDKVSTVKAGVAYYSGTIFGQRVVLCKSGVGKVNAAVTTQIL 64 IGLIGAM EE+ L+ E M+ + A V +Y GTI G RVVL KSGVGKVNAA+TTQ+L Sbjct: 4 IGLIGAMKEELALIKEQMDVISESTLATVCFYEGTIHGARVVLVKSGVGKVNAAMTTQLL 63 Query: 65 IDAFGVARVLFTGVAGAVHPELGIGDIVISSHCIQHDMDVTALGYPRGVIP-YQEVSSFP 123 ID F V ++FTGVAGA+ P L + D+VIS+ HDMD T LG+ RG IP ++ S F Sbjct: 64 IDHFHVDAIIFTGVAGALSPSLEVADLVISTSLQHHDMDGTPLGFKRGEIPMFERSSDFL 123 Query: 124 AAPDLVQLAEE-ACRALKQKSVTGVVLSGDQFIASAAVVAELREQLDGACAEMEGAAVAQ 182 A L +A + A +K V G ++SGDQFIA V EL E+ D C EMEGAAVAQ Sbjct: 124 ADEWLASVAYDVATNETGRKVVKGRIVSGDQFIADRHRVRELAEKFDAVCVEMEGAAVAQ 183 Query: 183 VCYMNGIPFVIIRSMSDKADGSAHVNYREFTVTASERSHAILEYMLKAM 231 V +N IPFV+IRS+SDKA+ A++++ EFT ASE+S I+ +LK + Sbjct: 184 VAALNQIPFVVIRSISDKANEEANISFAEFTKIASEQSQLIVSGVLKKL 232 >BH1279 [F] COG0775 Nucleoside phosphorylase Length = 231 Score = 169 bits (427), Expect = 5e-42, Method: Compositional matrix adjust. Identities = 94/218 (43%), Positives = 137/218 (62%), Gaps = 4/218 (1%) Query: 5 IGLIGAMDEEIKLLLESMEDKVSTVKAGVAYYSGTIFGQRVVLCKSGVGKVNAAVTTQIL 64 IG+IGAMDEE++LL + + A +Y+GTI G+ +VL KSG+GKVNAA+ T +L Sbjct: 4 IGIIGAMDEEVELLKSKLSNCSERTIAECEFYTGTIEGKEIVLLKSGIGKVNAAIGTTLL 63 Query: 65 IDAFGVARVLFTGVAGAVHPELGIGDIVISSHCIQHDMDVTALGYPRGVIPYQEVSSFPA 124 I F ++ TG AG + L +GD+ IS+ +D+D T GY G +P ++ +F Sbjct: 64 IQLFQPTAIINTGSAGGLDSSLHVGDLAISTEVRYNDVDATVFGYEFGQVP--QMPAFYQ 121 Query: 125 APD-LVQLAEEACRALKQKSVTGVVLSGDQFIASAAVVAELREQLD-GACAEMEGAAVAQ 182 D L+ +A EA + + S G++LSGD F++ AA+V +L+ + + C+EME A+AQ Sbjct: 122 PDDMLIDIAIEAAKTVGIPSKKGLILSGDSFMSDAALVEQLKRRFNYPLCSEMEAGAIAQ 181 Query: 183 VCYMNGIPFVIIRSMSDKADGSAHVNYREFTVTASERS 220 VC+ G+PFVIIRS+SD A A V+Y EF TAS S Sbjct: 182 VCHRFGVPFVIIRSLSDIAGAEAKVSYDEFLETASVNS 219 >DR1451 [F] COG0775 Nucleoside phosphorylase Length = 238 Score = 167 bits (424), Expect = 9e-42, Method: Compositional matrix adjust. Identities = 101/231 (43%), Positives = 139/231 (60%), Gaps = 1/231 (0%) Query: 1 MSEIIGLIGAMDEEIKLLLESMEDKVSTVKAGVAYYSGTIFGQRVVLCKSGVGKVNAAVT 60 + ++ +IGAMDEEI+LLL ++ + G + G + G V+L + G+GKVNAA+T Sbjct: 2 LGSMLAIIGAMDEEIELLLGELQGREDLTLPGATLHRGVLDGVPVLLTRGGIGKVNAALT 61 Query: 61 TQILIDAFGVARVLFTGVAGAVHPELGIGDIVISSHCIQHDMDVTALGYPRGVIPYQEVS 120 T L+ G V+FTGVAG VHP+L +GDIV+S+ +QHD+DVTALGY G +P + + Sbjct: 62 TAHLL-GQGATSVIFTGVAGGVHPDLRVGDIVVSTDLVQHDVDVTALGYDLGTLPGEPAT 120 Query: 121 SFPAAPDLVQLAEEACRALKQKSVTGVVLSGDQFIASAAVVAELREQLDGACAEMEGAAV 180 A A + + G V SGDQFIAS V L + ACAEMEGAAV Sbjct: 121 WTADARLRALAVAAAGDVGGVRVIEGRVASGDQFIASREGVGRLWKNFGAACAEMEGAAV 180 Query: 181 AQVCYMNGIPFVIIRSMSDKADGSAHVNYREFTVTASERSHAILEYMLKAM 231 AQVC G+PFV+IRS+SD AD A V+YR F + + ++ ML+ + Sbjct: 181 AQVCAKAGVPFVVIRSVSDTADHDAQVDYRTFMPLVARHAKQVVRGMLERL 231 >YPO3384 [F] COG0775 Nucleoside phosphorylase Length = 233 Score = 150 bits (379), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 91/228 (39%), Positives = 141/228 (61%), Gaps = 2/228 (0%) Query: 5 IGLIGAMDEEIKLLLESMEDKVSTVKAGVAYYSGTIFGQRVVLCKSGVGKVNAAVTTQIL 64 +G+IGAM+EE+ LL + +E++ + +AG Y+G + G V L KSG+GKV AA+ T +L Sbjct: 3 VGIIGAMEEEVTLLRDRIENRQTLARAGCEIYTGQLNGIDVALLKSGIGKVAAAMGTTLL 62 Query: 65 IDAFGVARVLFTGVAGAVHPELGIGDIVISSHCIQHDMDVTALGYPRGVIPYQEVSSFPA 124 ++ V+ TG AG + L +GDIV+S+ HD DVTA GY G + ++F A Sbjct: 63 LEHCQPDLVINTGSAGGLDSSLKVGDIVVSNEVRYHDADVTAFGYEPGQMAGCP-AAFVA 121 Query: 125 APDLVQLAEEACRALKQKSVTGVVLSGDQFIASAAVVAELREQL-DGACAEMEGAAVAQV 183 DL+ LAE + LK +V G++ SGD FI A +A +R A EME AA+ V Sbjct: 122 DEDLIALAENCIQQLKLNAVRGLICSGDAFINGAEPLARIRAAFPTVAAVEMEAAAIGHV 181 Query: 184 CYMNGIPFVIIRSMSDKADGSAHVNYREFTVTASERSHAILEYMLKAM 231 CY+ PFV++R++SD AD ++H+++ EF V A+++S +++ ML + Sbjct: 182 CYLFNTPFVVVRAISDVADQASHLSFEEFLVVAAKQSTLMIKAMLTTL 229 >STM0207 [F] COG0775 Nucleoside phosphorylase Length = 232 Score = 146 bits (368), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 83/228 (36%), Positives = 135/228 (59%), Gaps = 2/228 (0%) Query: 5 IGLIGAMDEEIKLLLESMEDKVSTVKAGVAYYSGTIFGQRVVLCKSGVGKVNAAVTTQIL 64 IG+IGAM+EE+ LL + ++++ + G Y+G + G V L KSG+GKV AA+ +L Sbjct: 3 IGIIGAMEEEVTLLRDKIDNRQTITLGGCEIYTGQLNGTEVALLKSGIGKVAAALGATLL 62 Query: 65 IDAFGVARVLFTGVAGAVHPELGIGDIVISSHCIQHDMDVTALGYPRGVIPYQEVSSFPA 124 ++ ++ TG AG + L +GDIV+S HD DVTA GY G +P + F A Sbjct: 63 LEHCKPDVIINTGSAGGLASTLKVGDIVVSDEARYHDADVTAFGYEYGQLPGCP-AGFKA 121 Query: 125 APDLVQLAEEACRALKQKSVTGVVLSGDQFIASAAVVAELREQL-DGACAEMEGAAVAQV 183 L+ AE R L +V G+++SGD FI + +A++R D EME A+A V Sbjct: 122 DDKLIAAAESCIRELNLNAVRGLIVSGDAFINGSVGLAKIRHNFPDAVAVEMEATAIAHV 181 Query: 184 CYMNGIPFVIIRSMSDKADGSAHVNYREFTVTASERSHAILEYMLKAM 231 C+ +PFV++R++SD AD +H+++ EF A+++S ++E +++ + Sbjct: 182 CHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQSTLMVETLVQKL 229 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.134 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,845,981 Number of extensions: 652555 Number of successful extensions: 1366 Number of sequences better than 1.0e-05: 22 Number of HSP's gapped: 1343 Number of HSP's successfully gapped: 22 Length of query: 231 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 128 Effective length of database: 26,499,029 Effective search space: 3391875712 Effective search space used: 3391875712 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 114 (48.5 bits)