BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0547.0019.p (152 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ML0381 [O] COG0459 Chaperonin GroEL (HSP60 family) 155 2e-38 CAC3105 [J] COG0522 Ribosomal protein S4 and related proteins 137 7e-33 CAC1594 [J] COG0522 Ribosomal protein S4 and related proteins 131 3e-31 CAC1478 [J] COG0522 Ribosomal protein S4 and related proteins 127 4e-30 STM1473 [M] COG3203 Outer membrane protein (porin) 125 2e-29 >ML0381 [O] COG0459 Chaperonin GroEL (HSP60 family) Length = 203 Score = 155 bits (393), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 78/153 (50%), Positives = 105/153 (68%), Gaps = 1/153 (0%) Query: 1 MQLLEKQKLRHMYGLGEKQFRTLFSKAQKL-QGIAGENFMFLLESRLDNLVYRLGFANSR 59 +QL EKQKLR YGL E+QFR L+ A K+ +G G NFM LLE RLDN+V+RLG A +R Sbjct: 49 LQLAEKQKLRFSYGLSERQFRNLYIAATKVKEGTVGFNFMTLLEQRLDNVVFRLGLATTR 108 Query: 60 AGARQLVSHGHVTVNGKKVDIASYRVSVGDVIGLRERSRAMASIKEALENRSHLPGYLEY 119 ARQ V+HGH+ V+GK+VDI S+RV G VI +RE+S + +I EA+E ++ + Sbjct: 109 RQARQFVNHGHILVDGKRVDIPSFRVQPGQVISVREKSMKVPAILEAVEATKGRANFVSF 168 Query: 120 ADGSFEGKYIRLPERAELSQDIDEKQIVEFYNR 152 EG +RLPER E++ +I+E IVEFYN+ Sbjct: 169 DADKLEGTLVRLPERDEINPEINEALIVEFYNK 201 >CAC3105 [J] COG0522 Ribosomal protein S4 and related proteins Length = 206 Score = 137 bits (345), Expect = 7e-33, Method: Compositional matrix adjust. Identities = 71/152 (46%), Positives = 101/152 (66%) Query: 1 MQLLEKQKLRHMYGLGEKQFRTLFSKAQKLQGIAGENFMFLLESRLDNLVYRLGFANSRA 60 +QL EKQK + +YG+ EKQFR + +A++++GI+GEN + LLE RLDN+ Y+LG+ +SR Sbjct: 55 LQLREKQKAKRIYGVLEKQFRIYYKRAERIKGISGENLLKLLELRLDNVAYKLGYGSSRK 114 Query: 61 GARQLVSHGHVTVNGKKVDIASYRVSVGDVIGLRERSRAMASIKEALENRSHLPGYLEYA 120 ARQLV+HGH VNGKKVDI SY + V +V+ + E+SR K EN LP ++E Sbjct: 115 EARQLVTHGHFLVNGKKVDIPSYTLKVNEVVSVSEKSRGTEKFKTFAENPKTLPAWIEAN 174 Query: 121 DGSFEGKYIRLPERAELSQDIDEKQIVEFYNR 152 +FE K + P R ++ I E IVE Y++ Sbjct: 175 YDNFEAKIVAEPNREDIDTPIKETLIVELYSK 206 >CAC1594 [J] COG0522 Ribosomal protein S4 and related proteins Length = 196 Score = 131 bits (330), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 70/154 (45%), Positives = 97/154 (62%), Gaps = 3/154 (1%) Query: 1 MQLLEKQKLRHMYGLGEKQFRTLFSKAQKLQGIAGENFMFLLESRLDNLVYRLGFANSRA 60 M L+EKQKL+ YG+ EKQF A K + +G + + +LE RLDN+VYR+GFANS Sbjct: 44 MHLIEKQKLKAYYGVLEKQFSRYVKAAMKNKEASGSSLLKILECRLDNVVYRIGFANSIR 103 Query: 61 GARQLVSHGHVTVNGKKVDIASYRVSVGDVIGLRERSRAMASIKEALENRS--HLPGYLE 118 ARQ+VSHG + VNGKK+DI SY V VGDV+ L+E+ R N S ++P Y+E Sbjct: 104 QARQMVSHGLILVNGKKLDIPSYEVQVGDVVSLKEKHRQNEMFSNNFMNLSTFNVP-YIE 162 Query: 119 YADGSFEGKYIRLPERAELSQDIDEKQIVEFYNR 152 F GK +RLP E+ ++E ++EFY++ Sbjct: 163 KNPDEFSGKLLRLPNIDEIPIKVNEVAVIEFYSK 196 >CAC1478 [J] COG0522 Ribosomal protein S4 and related proteins Length = 192 Score = 127 bits (320), Expect = 4e-30, Method: Compositional matrix adjust. Identities = 67/151 (44%), Positives = 96/151 (63%) Query: 2 QLLEKQKLRHMYGLGEKQFRTLFSKAQKLQGIAGENFMFLLESRLDNLVYRLGFANSRAG 61 QLLEKQKLR YG+ EKQF+ + A K + + + + LE RLDNLVYRLGF ++ Sbjct: 41 QLLEKQKLRAYYGVLEKQFKKIVFNALKSKEKSEDILVQSLERRLDNLVYRLGFGSTLRE 100 Query: 62 ARQLVSHGHVTVNGKKVDIASYRVSVGDVIGLRERSRAMASIKEALENRSHLPGYLEYAD 121 ARQ+VSHGH+ VNG+KVDI SY+V++GD + LR +SR + + Y+E Sbjct: 101 ARQMVSHGHILVNGQKVDIPSYKVNIGDEVSLRSKSRKIQTYASNFTTIIPAVTYIEKDT 160 Query: 122 GSFEGKYIRLPERAELSQDIDEKQIVEFYNR 152 SF G+ IRLP+ E+ + +++EFY++ Sbjct: 161 ESFSGRLIRLPKSVEVPVMVKYSKVLEFYSK 191 >STM1473 [M] COG3203 Outer membrane protein (porin) Length = 377 Score = 125 bits (315), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 10/156 (6%), Positives = 25/156 (16%), Gaps = 5/156 (3%) Query: 1 MQLLEKQKLRHMYGLGEKQFRTLFSKAQKLQGIAGENFMFLLESRLDNLVYRLGFANSRA 60 + K + Q + + G + Sbjct: 55 TYMRVGFKGETQINDQLTGYGQWEYNVQANTTEGEGANSWTRLAFAGLKFGDYGSFDYGR 114 Query: 61 GARQLVSHGHVTVNGKKVDIASYRVSVGDVIGLRERSRAMASIKEALE---NRSHLPGYL 117 L T + SY + + G + L Y Sbjct: 115 NYGVLYDVEGWTDMLPEFGGDSYTYADNFMTGRANGVATYRNTDFFGLVDGLNFALQ-YQ 173 Query: 118 EYADGSFEGKYIRLPERAELSQ-DIDEKQIVEFYNR 152 + + + + + + Sbjct: 174 GANENQVSHEQEGTNNGGDRNVKNANGDGFGISSTY 209 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.137 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 11,080,536 Number of extensions: 419819 Number of successful extensions: 885 Number of sequences better than 1.0e-05: 13 Number of HSP's gapped: 882 Number of HSP's successfully gapped: 13 Length of query: 152 Length of database: 38,378,431 Length adjustment: 97 Effective length of query: 55 Effective length of database: 27,191,033 Effective search space: 1495506815 Effective search space used: 1495506815 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 111 (47.4 bits)