BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0547.0018.p (521 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ytrP_2 [T] COG2199 FOG: GGDEF domain 179 1e-44 TP0981_2 [T] COG2199 FOG: GGDEF domain 122 2e-27 ECs1925_3 [T] COG2199 FOG: GGDEF domain 117 6e-26 Z2421_3 [T] COG2199 FOG: GGDEF domain 117 6e-26 ydaM_3 [T] COG2199 FOG: GGDEF domain 117 6e-26 >BS_ytrP_2 [T] COG2199 FOG: GGDEF domain Length = 199 Score = 179 bits (453), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 91/198 (45%), Positives = 128/198 (64%), Gaps = 1/198 (0%) Query: 316 MLTHREPHFFSYDSYRLLQAMAGHIGLAVGNARLHAEVRRLANRDSLTGLYARHYLDEII 375 +L +E + F+++ Y+L QA+ H LAV N+ L + L D LT LY+R YLDE I Sbjct: 2 VLLKQELYAFTFEMYKLFQALIHHATLAVTNSMLRDRLEHLVKTDQLTELYSRAYLDEKI 61 Query: 376 KDKQITEFCGSLIVVDIDQFKMVNDTYGHQKGDKILRQVSEIVKSSIRQGDIAARWGGEE 435 + G I+VDID FK VNDTYGHQ GD+IL QV+ ++KS+IR+ D+ ARWGGEE Sbjct: 62 QYSMKIHQKGVFILVDIDNFKNVNDTYGHQTGDEILIQVASVIKSNIRKHDVGARWGGEE 121 Query: 436 LAVYLPQLGVQQAVFVAERIRKQVMSETDPRVTVSCGIAEWSWTDDKVSVESLFYRADMA 495 LA+YLP + V + ER+ V T P VT+SCGI+ W+ T+ K S++ L + AD A Sbjct: 122 LAIYLPNVPVAVGKRITERLVYAVRKNTKPEVTISCGISCWT-TETKKSLKELVHEADEA 180 Query: 496 LYEAKNNGRNQVVLDTKI 513 LY AK +G+N++++ I Sbjct: 181 LYSAKRSGKNRLMIHDSI 198 >TP0981_2 [T] COG2199 FOG: GGDEF domain Length = 177 Score = 122 bits (305), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 70/165 (42%), Positives = 100/165 (60%), Gaps = 11/165 (6%) Query: 357 ANRDSLTGLYARHYLDEIIK---DKQITEFCGSLIVVDIDQFKMVNDTYGHQKGDKILRQ 413 A D +T + +HY ++ D T+ S++++DID FK +NDT+GH GD +L+ Sbjct: 8 ATTDMMTQMKLKHYFFAMLTAKLDTLSTQETVSVLMLDIDFFKQINDTHGHLCGDLVLQH 67 Query: 414 VSEIVKSSIRQGDIAARWGGEELAVYLPQLGVQQAVFVAERIRKQVMSETDP------RV 467 V+EI++S R DIA+R+GGEE + L ++A VAERIR T P RV Sbjct: 68 VAEIIRSCTRPCDIASRYGGEEFMLMLSNNSSREAAHVAERIRVATEQLTIPYHEVSIRV 127 Query: 468 TVSCGIAEWSWTDDKVSVESLFYRADMALYEAKNNGRNQVVLDTK 512 TVS G+AE + ++ S E+L RAD ALY+AK NGRN+VV+ K Sbjct: 128 TVSAGVAE--YLPNQESAETLIKRADSALYQAKQNGRNKVVISEK 170 >ECs1925_3 [T] COG2199 FOG: GGDEF domain Length = 170 Score = 117 bits (292), Expect = 6e-26, Method: Compositional matrix adjust. Identities = 65/168 (38%), Positives = 98/168 (58%), Gaps = 10/168 (5%) Query: 348 RLHAEVRRLANRDSLTGLYARHYLDEIIKDKQITEFC----GSLIVVDIDQFKMVNDTYG 403 RL ++ A+ D++TGL R I + Q+ SL+++D D+FK +ND YG Sbjct: 4 RLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQMQHLAIAQDYSLLLIDTDRFKHINDLYG 63 Query: 404 HQKGDKILRQVSEIVKSSIRQGDIAARWGGEELAVYLPQLGVQQAVFVAERIRKQVMSET 463 H KGD++L ++ ++S R+GD+ RWGGEE + LP+ + A+ +AE IR V + Sbjct: 64 HSKGDEVLCALARTLESCARKGDLVFRWGGEEFVLLLPRTPLDTALSLAETIRVSVAKVS 123 Query: 464 D---PRVTVSCGIAEWSWTDDKVSVESLFYRADMALYEAKNNGRNQVV 508 PR TVS G+A + S++ LF R D ALY AKN+GRN+V+ Sbjct: 124 ISGLPRFTVSIGVAHHEGNE---SIDELFKRVDDALYRAKNDGRNRVL 168 >Z2421_3 [T] COG2199 FOG: GGDEF domain Length = 170 Score = 117 bits (292), Expect = 6e-26, Method: Compositional matrix adjust. Identities = 65/168 (38%), Positives = 98/168 (58%), Gaps = 10/168 (5%) Query: 348 RLHAEVRRLANRDSLTGLYARHYLDEIIKDKQITEFC----GSLIVVDIDQFKMVNDTYG 403 RL ++ A+ D++TGL R I + Q+ SL+++D D+FK +ND YG Sbjct: 4 RLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQMQHLAIAQDYSLLLIDTDRFKHINDLYG 63 Query: 404 HQKGDKILRQVSEIVKSSIRQGDIAARWGGEELAVYLPQLGVQQAVFVAERIRKQVMSET 463 H KGD++L ++ ++S R+GD+ RWGGEE + LP+ + A+ +AE IR V + Sbjct: 64 HSKGDEVLCALARTLESCARKGDLVFRWGGEEFVLLLPRTPLDTALSLAETIRVSVAKVS 123 Query: 464 D---PRVTVSCGIAEWSWTDDKVSVESLFYRADMALYEAKNNGRNQVV 508 PR TVS G+A + S++ LF R D ALY AKN+GRN+V+ Sbjct: 124 ISGLPRFTVSIGVAHHEGNE---SIDELFKRVDDALYRAKNDGRNRVL 168 >ydaM_3 [T] COG2199 FOG: GGDEF domain Length = 170 Score = 117 bits (292), Expect = 6e-26, Method: Compositional matrix adjust. Identities = 65/168 (38%), Positives = 98/168 (58%), Gaps = 10/168 (5%) Query: 348 RLHAEVRRLANRDSLTGLYARHYLDEIIKDKQITEFC----GSLIVVDIDQFKMVNDTYG 403 RL ++ A+ D++TGL R I + Q+ SL+++D D+FK +ND YG Sbjct: 4 RLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQMQHLAIAQDYSLLLIDTDRFKHINDLYG 63 Query: 404 HQKGDKILRQVSEIVKSSIRQGDIAARWGGEELAVYLPQLGVQQAVFVAERIRKQVMSET 463 H KGD++L ++ ++S R+GD+ RWGGEE + LP+ + A+ +AE IR V + Sbjct: 64 HSKGDEVLCALARTLESCARKGDLVFRWGGEEFVLLLPRTPLDTALSLAETIRVSVAKVS 123 Query: 464 D---PRVTVSCGIAEWSWTDDKVSVESLFYRADMALYEAKNNGRNQVV 508 PR TVS G+A + S++ LF R D ALY AKN+GRN+V+ Sbjct: 124 ISGLPRFTVSIGVAHHEGNE---SIDELFKRVDDALYRAKNDGRNRVL 168 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.136 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 36,521,808 Number of extensions: 1480776 Number of successful extensions: 2942 Number of sequences better than 1.0e-05: 140 Number of HSP's gapped: 2783 Number of HSP's successfully gapped: 140 Length of query: 521 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 410 Effective length of database: 25,576,357 Effective search space: 10486306370 Effective search space used: 10486306370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 118 (50.1 bits)