BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0547.0014.p (378 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3246 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) 490 e-138 lin1644 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) 461 e-130 DR0585 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) 160 2e-39 VNG0186G [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) 156 6e-38 VNG2449G [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) 154 2e-37 >BH3246 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) Length = 371 Score = 490 bits (1261), Expect = e-138, Method: Compositional matrix adjust. Identities = 234/358 (65%), Positives = 277/358 (77%) Query: 8 MKDPRIQKLAENLVGYSVNVQPGENVLVEMIGSERDLIKAVVEEVGKAGGHAFVQLTDRT 67 M+DPRI +LA+NL+ YS+ +QPGE VL+E G +RDL+ A+VE+ +AGG+ FV L D Sbjct: 1 MRDPRITELADNLINYSIRLQPGEKVLIENFGLQRDLVTALVEKAYEAGGYPFVLLKDYQ 60 Query: 68 VLRSMLQYATREGLEAWAEIDLNRMKQMDCYIGIRAGENVNDLSDVPEENMKLYNSLYSH 127 V RS+L A E LE A ++ M+++D YIG+R+G+N+++LSDVP + + L+ Sbjct: 61 VDRSLLLGAQEEQLELIAHVEATMMEKVDAYIGLRSGDNISELSDVPGDKLALWGKTVGT 120 Query: 128 PVHSEQRVKHTKWVVLRYPNASMAQLANTTTEAFEDFYFEVCNLDYAKMDRAQDALAELM 187 VH E RV KWVVLRYPN SMAQLAN +T FEDFYF VCNLDY KM A DAL LM Sbjct: 121 KVHREIRVPKKKWVVLRYPNHSMAQLANMSTAGFEDFYFNVCNLDYGKMSHAMDALVALM 180 Query: 188 RRTDKVRISGPGTELSFSIKGIGAEKCSGHRNIPDGEVYSAPVRDSVNGTISYNAATIYN 247 RTDKV+I GPGT+L+FSIKGI A KC+G NIPDGEVY+APVRDSVNGTISYN T Y Sbjct: 181 NRTDKVQIKGPGTDLTFSIKGIPAVKCAGEMNIPDGEVYTAPVRDSVNGTISYNTPTPYQ 240 Query: 248 GVTFENVKFKFENGKIVEATSNDPVRLNEILDSDEGARHIGEFAIGFNPYILHPMKDILF 307 G TFE V F F++GKI+EAT+ND R+N ILDSDEGAR+IGEFAIG NPYILHPMKDILF Sbjct: 241 GFTFEQVSFTFKDGKIIEATANDTERINRILDSDEGARYIGEFAIGVNPYILHPMKDILF 300 Query: 308 DEKIAGSLHFTPGQAYDVTDNGNRSSIHWDLVLIQRPEYGGGEIYFDDVLIRKDGIFV 365 DEKI GS HFTPGQ YD NGN S+IHWD+V+IQRPEYGGGEIYFDD LIRKDG FV Sbjct: 301 DEKIDGSFHFTPGQCYDDAFNGNHSTIHWDIVMIQRPEYGGGEIYFDDQLIRKDGRFV 358 >lin1644 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) Length = 370 Score = 461 bits (1186), Expect = e-130, Method: Compositional matrix adjust. Identities = 219/358 (61%), Positives = 274/358 (76%), Gaps = 1/358 (0%) Query: 8 MKDPRIQKLAENLVGYSVNVQPGENVLVEMIGSERDLIKAVVEEVGKAGGHAFVQLTDRT 67 MKD RI+KLA NL+ YSV + GE VL+E G +++L+ A+VEE KAGG FV L + Sbjct: 1 MKDARIEKLAHNLINYSVKLGAGEKVLIENFGVQKELVMALVEEAYKAGGFPFVSLKEPQ 60 Query: 68 VLRSMLQYATREGLEAWAEIDLNRMKQMDCYIGIRAGENVNDLSDVPEENMKLYNSLYSH 127 + R+M+ A E AE + N MK+MD YIG+RAG+N+N+ SDVP + +K++ Sbjct: 61 IDRAMMLGADSAQYEKIAEFEGNVMKEMDAYIGLRAGDNINETSDVPADKLKIHGETVGK 120 Query: 128 PVHSEQRVKHTKWVVLRYPNASMAQLANTTTEAFEDFYFEVCNLDYAKMDRAQDALAELM 187 +HS+ RVK TKWVVLRYP++SMAQLA +T FEDFYF+VCNLDY KM A D L ELM Sbjct: 121 -MHSDIRVKQTKWVVLRYPSSSMAQLAKMSTVGFEDFYFDVCNLDYGKMSTAMDGLVELM 179 Query: 188 RRTDKVRISGPGTELSFSIKGIGAEKCSGHRNIPDGEVYSAPVRDSVNGTISYNAATIYN 247 +TDKV + GPGT+L+FSIK I A KC+G NIPDGEV++APVRDS+NGT++YN + Y Sbjct: 180 NKTDKVHLVGPGTDLTFSIKDIPAIKCAGEMNIPDGEVFTAPVRDSINGTLTYNTPSPYQ 239 Query: 248 GVTFENVKFKFENGKIVEATSNDPVRLNEILDSDEGARHIGEFAIGFNPYILHPMKDILF 307 G TFENV F F++GKI++AT+ND R+N++LD+DEGAR +GEFAIG NP+I PM+DILF Sbjct: 240 GFTFENVSFTFKDGKIIKATANDTDRINKVLDTDEGARFVGEFAIGVNPFIHEPMQDILF 299 Query: 308 DEKIAGSLHFTPGQAYDVTDNGNRSSIHWDLVLIQRPEYGGGEIYFDDVLIRKDGIFV 365 DEKI GS HFTPGQ YD NGN+S+IHWDLV IQR +YGGGEIYFDDVLIRKDGIFV Sbjct: 300 DEKIEGSFHFTPGQCYDEAFNGNQSAIHWDLVNIQRADYGGGEIYFDDVLIRKDGIFV 357 >DR0585 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) Length = 380 Score = 160 bits (406), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 111/366 (30%), Positives = 172/366 (46%), Gaps = 18/366 (4%) Query: 10 DPRIQKLAENLVGYSVNVQPGENVLVEMIGSERDLIKAVVEEVGKAGGHAFVQLTDRTVL 69 DP ++ AE L Y ++ QPGE +LV + L+ A + +AG V+L+ Sbjct: 22 DP--ERHAELLAHYCLSAQPGERLLVGASTAAAPLVAATSRALLRAGARPAVRLSYPGQD 79 Query: 70 RSMLQYATREGLEAWAEIDLNRMKQMDCYIGIRAGENVNDLSDVPEENMKLYNSLYSHPV 129 A L+ + +L+ M+ MD + I E + + + + Sbjct: 80 EDFAALAADAVLDEVPQGELDDMRGMDGSLRILTPEAAQPADAARAARLLASRAELAA-L 138 Query: 130 HSEQRVKHTKWVVLRYPNASMAQLANTTTEAFEDFYFEVCNLD-------YAKMDRAQDA 182 S++ KW + YP A A T FEDF LD + ++ Q Sbjct: 139 RSQK-----KWSLTLYPTPHAAAQAGMTLAEFEDFVMRAMFLDRPDPVAAWGEVRELQAR 193 Query: 183 LAELMRRTDKVRISGPGTELSFSIKGIGAEKCSGHRNIPDGEVYSAPVRDSVNGTISYNA 242 L E + R D VRI PGT+L+ + G G RN+P GEV++ P+ DS G +++ Sbjct: 194 LIERLSRADVVRIEAPGTDLTLRVGGRIWSNSDGKRNMPSGEVFTGPLEDSAEGVVTFTV 253 Query: 243 ATIYNGVTFENVKFKFENGKIVEATSND-PVRLNEILDSDEGARHIGEFAIGFNPYILHP 301 Y G T + F G++VEA++ + L+ LD+D GAR +GE IG N I Sbjct: 254 PASYQGQTVRGARLVFRAGQVVEASAEEGEAVLHAALDTDPGARRLGELGIGTNFGIQRG 313 Query: 302 MKDILFDEKIAGSLHFTPGQAYDVTDNGNRSSIHWDLVLIQRPE--YGGGEIYFDDVLIR 359 + +ILFDEKI G++H G++Y T NRS++HWDL+ R GGG + D + + Sbjct: 314 IGNILFDEKIGGTVHLAIGKSYPETGGLNRSAVHWDLITDLRAASGAGGGRLLLDGEVWQ 373 Query: 360 KDGIFV 365 + G F+ Sbjct: 374 EGGRFL 379 >VNG0186G [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) Length = 363 Score = 156 bits (394), Expect = 6e-38, Method: Compositional matrix adjust. Identities = 119/370 (32%), Positives = 184/370 (49%), Gaps = 29/370 (7%) Query: 10 DPRIQKLAENLVGYSVNVQPGENVLVEMIGSERDLIKAVVEEVGKAGGHAFVQLTDRTVL 69 D R+++ AE LV +V+ G+ V + L+ A+ E +G+ G R L Sbjct: 2 DTRVRRHAEVLVDQCADVRRGDMVTIRASRQAEALVVALYEALGQRGA--------RPSL 53 Query: 70 RSMLQYATREGLEAWAEID-------LNRMKQMDCYIGIRAGENVNDLSDVPEENMKLYN 122 L ATR A E D L M++ D I G N + SDVP E M Sbjct: 54 SWSLPRATRAYARAMDEADYTTKDHELAAMEETDVVFLIGGGPNAFETSDVPPE-MSQAG 112 Query: 123 SLYSHPVHSEQRVKHTKWVVLRYPNASMAQLANTTTEAFEDFYFEVCNLDYAKMDRAQDA 182 S P+ SEQR+ T+WV+ +P A+ AQ A +T A+E+F ++ N D+A Q Sbjct: 113 SRAHKPI-SEQRLD-TRWVITTHPTAAGAQRAGMSTAAYEEFVYDAVNKDWAAQSEFQSQ 170 Query: 183 LAELMRRTDKVRI-SGPGTELSFSIKGIGAEKCSGHRNIPDGEVYSAPVRDSVNGTISYN 241 L E++ + V I SG T+++ + G+ A G N+P GEV++APV DSV G + ++ Sbjct: 171 LVEILDPAETVHIVSGDTTDITMRVDGMRACNDDGDHNMPAGEVFTAPVPDSVAGEVYFD 230 Query: 242 AATIYNGVTFENVKFKFENGKIV--EATSNDPVRLNEILDSDEGARHIGEFAIGFNPYIL 299 + G FE+G +V A N+ V L IL++D GAR +GE IG N I Sbjct: 231 KPLMRQGNEIRGAALTFEDGDVVSFSAEQNEDV-LAGILNTDAGARRLGELGIGMNRGID 289 Query: 300 HPMKDILFDEKIAGSLHFTPGQAYD--VTDNG--NRSSIHWDLVLIQRPEYGGGEIYFDD 355 ++LFDEK+ ++H G A D V ++ N S++H D+++ + I D Sbjct: 290 RFTYNMLFDEKMGDTVHLAVGGAIDECVPEDCEFNDSAVHTDMIVDMSED---SFIEIDG 346 Query: 356 VLIRKDGIFV 365 +++++G FV Sbjct: 347 EIVQRNGTFV 356 >VNG2449G [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) Length = 369 Score = 154 bits (389), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 111/370 (30%), Positives = 187/370 (50%), Gaps = 23/370 (6%) Query: 10 DPRIQKLAENLVGYSVNVQPGENVLVEMIGSERDLIKAVVEEVGKAGGHAFVQL------ 63 D RI++ A +V +++QP ENV+V + DL A+ E++G+ G + + Sbjct: 2 DSRIRQHAAVIVDRCIDLQPDENVVVSLPPVAEDLRVALYEKIGEVGANPVMLARGDRGI 61 Query: 64 -TDRTVLRSMLQYATREGLEAWAEIDLNRMKQMDCYIGIRAGENVNDLSDVPEENMKLYN 122 TDR R+ LQ E L + L + D + R NV + SDV E + Sbjct: 62 GTDRAA-RAYLQAIDAEDLSTPEHL-LQLYEHADAAVVGRPHRNVTEQSDVSTELGAAFA 119 Query: 123 SLYSHPVHSEQRVKHTKWVVLRYPNASMAQLANTTTEAFEDFYFEVCNLDYAKMDRAQDA 182 + +++ TKW + +YP + AQLA +T A+EDF ++ N D+ ++ Q Sbjct: 120 EAHRDVLNAR---LSTKWCLTQYPAPADAQLAGMSTAAYEDFVYDAVNKDWDAVEDHQQQ 176 Query: 183 LAELMRRTDKVRI-SGPGTELSFSIKGIGAEKCSGHRNIPDGEVYSAPVRDSVNGTISYN 241 + +++ ++VRI SG T+++ S+ + G N+P GEV++APV DSV G + ++ Sbjct: 177 MVDILNPAEEVRIVSGDTTDVTMSVADMTTLNDCGSNNLPAGEVFTAPVPDSVEGEVVFD 236 Query: 242 AATIYNGVTFENVKFKFENGKIV--EATSNDPVRLNEILDSDEGARHIGEFAIGFNPYIL 299 + + FE+G++V A N+ V L ILD+D+GAR +GE IG N I Sbjct: 237 KPVYHQAREIQGAALTFEDGEVVSFSAEQNEDV-LAAILDTDDGARRLGELGIGMNRDID 295 Query: 300 HPMKDILFDEKIAGSLHFTPGQAYDVTDN----GNRSSIHWDLVLIQRPEYGGGEIYFDD 355 ++LFDEK+ ++H G+AY T N S+ H D+++ + I D Sbjct: 296 RFTYNMLFDEKMGDTVHMALGRAYPETVGEGVEQNDSAKHVDMIVDMSED---SFIEVDG 352 Query: 356 VLIRKDGIFV 365 +++++G FV Sbjct: 353 EIVQRNGTFV 362 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,461,361 Number of extensions: 1194515 Number of successful extensions: 2993 Number of sequences better than 1.0e-05: 14 Number of HSP's gapped: 2968 Number of HSP's successfully gapped: 14 Length of query: 378 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 270 Effective length of database: 25,922,359 Effective search space: 6999036930 Effective search space used: 6999036930 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)