BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0547.0008.p (414 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_spoVE [D] COG0772 Bacterial cell division membrane protein 228 2e-59 BH2566 [D] COG0772 Bacterial cell division membrane protein 208 2e-53 lin1059 [D] COG0772 Bacterial cell division membrane protein 195 1e-49 BS_ylaO [D] COG0772 Bacterial cell division membrane protein 181 1e-45 HI1137 [D] COG0772 Bacterial cell division membrane protein 178 2e-44 >BS_spoVE [D] COG0772 Bacterial cell division membrane protein Length = 366 Score = 228 bits (580), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 134/349 (38%), Positives = 200/349 (57%), Gaps = 21/349 (6%) Query: 49 ASEKFGNDPFYFVKRQIIWVVLGSFVMFVTMNIHYSKFKKWYAPIFMIT---LVLLLFVA 105 A KF +D F+F KRQ+++ +G MF MN+ Y ++ W + +I LVL+L Sbjct: 35 ADYKF-DDSFFFAKRQLLFAGIGVIAMFFIMNVDYWTWRTWSKLLMVICFFLLVLVLIPG 93 Query: 106 FAERINGAKSWLNIGGLGIQPTEXXXXXXXXXXXXXXXXXGERLRDLRTGYIPVMVIVGI 165 NG++SW+ +G IQP+E + + R G++P + IV Sbjct: 94 VGMVRNGSRSWIGVGAFSIQPSEFMKLAMIAFLAKFLSEKQKNITSFRRGFVPALGIVFS 153 Query: 166 VAGLIMMQPDLGSCLILVATSGLVIYAGGASMKHIMASIXXXXXXXXXXXXXXXXIDSLS 225 +IM QPDLG+ ++V T ++I+ GA + H + + LS Sbjct: 154 AFLIIMCQPDLGTGTVMVGTCIVMIFVAGARIAHFV----------------FLGLIGLS 197 Query: 226 PPSETAAVSKDYKKDRISAFINPEADPEDGGYNILQSLIALGEGGANGSGFGQSIQKLHY 285 +S Y+ RI++++NP DP G+ I+QSL A+G GG G G GQS QK Y Sbjct: 198 G-FVGLVLSAPYRIKRITSYLNPWEDPLGSGFQIIQSLYAVGPGGLFGMGLGQSRQKFFY 256 Query: 286 LPYPYTDFIFAVVGEELGFIGTSLFLLAYLYLIWRGILIALRCTDPFGTLVGIGVMGLIA 345 LP P TDFIFA++ EELGFIG +L LL + L+WRGI IAL D +G+ V +G++ +IA Sbjct: 257 LPEPQTDFIFAILSEELGFIGGTLILLLFSVLLWRGIRIALGAPDLYGSFVAVGIISMIA 316 Query: 346 IQAFVNIGGVTNTIPLTGVTLPFISYGGSSLLVTMLSMGIMLSISRETN 394 IQ +NIG VT IP+TG+TLPF+SYGGSSL + ++++G++L++SR + Sbjct: 317 IQVMINIGVVTGLIPVTGITLPFLSYGGSSLTLMLMAVGVLLNVSRYSR 365 >BH2566 [D] COG0772 Bacterial cell division membrane protein Length = 366 Score = 208 bits (529), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 127/383 (33%), Positives = 203/383 (53%), Gaps = 25/383 (6%) Query: 16 KRGTPDFQXXXXXXXXXXXXXXMVFXXXXXLTLASEKFGNDPFYFVKRQIIWVVLGSFVM 75 ++ PD+ MV+ A+ +F +D F+F KRQ+ + +G M Sbjct: 4 QKAAPDYLLVVATVALLIIGLIMVYSASE--AWATYRF-DDGFFFAKRQLFFASVGFIAM 60 Query: 76 FVTMNIHYSKFKKWYAPIFMITLVLLLFV---AFAERINGAKSWLNIGGLGIQPTEXXXX 132 M + Y ++ W + ++ VLL+ V GA+SWL +G IQP+E Sbjct: 61 LFMMRVEYWTWRVWAKLMVIVCFVLLIIVLIPGVGLVRGGARSWLGVGAFSIQPSEFMKM 120 Query: 133 XXXXXXXXXXXXXGERLRDLRTGYIPVMVIVGIVAGLIMMQPDLGSCLILVATSGLVIYA 192 + + + G +P + +V + G+IM+QPDLG+ ++V T ++I+ Sbjct: 121 AMIVFLAKFLAENQKLITSFKKGLLPSLSLVMLAFGMIMLQPDLGTGAVMVGTCVVMIFV 180 Query: 193 GGASMKH-IMASIXXXXXXXXXXXXXXXXIDSLSPPSETAAVSKDYKKDRISAFINPEAD 251 GA + H +M I +S Y+ RI++F++P +D Sbjct: 181 AGARILHFVMLGIVGMAGFAALI------------------ISAPYRIKRITSFLDPWSD 222 Query: 252 PEDGGYNILQSLIALGEGGANGSGFGQSIQKLHYLPYPYTDFIFAVVGEELGFIGTSLFL 311 P G+ I+QSL A+G GG G G G+S QK +YLP P TDFIFA++ EELGF+G + Sbjct: 223 PLGSGFQIIQSLYAIGPGGLLGLGLGESRQKYYYLPEPQTDFIFAILSEELGFLGGCFVI 282 Query: 312 LAYLYLIWRGILIALRCTDPFGTLVGIGVMGLIAIQAFVNIGGVTNTIPLTGVTLPFISY 371 + ++WRGI IAL D FG+ + G++ ++AIQ +NIG VT +P+TG+TLP +SY Sbjct: 283 ALFGIVLWRGIRIALGAPDLFGSFLATGIVAMVAIQVMINIGVVTGLMPVTGITLPLLSY 342 Query: 372 GGSSLLVTMLSMGIMLSISRETN 394 GGSSL + ++S+G++L++SR T Sbjct: 343 GGSSLTLMLVSLGVLLNVSRYTR 365 >lin1059 [D] COG0772 Bacterial cell division membrane protein Length = 331 Score = 195 bits (495), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 0/360 (0%), Positives = 0/360 (0%), Gaps = 11/360 (3%) Query: 59 YFVKRQIIWVVLGSFVMFVTMNIHYSKFKKWYAPIFMITL----VLLLFVAFAERINGAK 114 + +Q + + + S + P L + A ++ Sbjct: 45 TSLTQQQRAELW-AGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQL 103 Query: 115 SWLNIGGLGIQPTEXXXXXXXXXXXXXXXXXGERLRDLRTGYIPVMVIVGIVAGLIMMQP 174 G Q + L MVI +P Sbjct: 104 GEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREATDRP 163 Query: 175 DLGSCLILVATSGLVIYAGGASMKHIMASIXXXXXXXXXXXXXXXXIDSLSPPSETAAVS 234 L + + + A + + + Sbjct: 164 LLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKL----FNHLVTWLQ 219 Query: 235 KDYKKDRISAFINPEADPEDGGYNILQSLIALGEGGANGSGFGQSIQKLHYLPYPYTDFI 294 + + L AL G + + Sbjct: 220 ARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDILNQL 279 Query: 295 FAVVGEELGFIGTSLFLLAYLYLIWRGILIALRCTDPFGTLVGIGVMGLIAIQAFVNIGG 354 Sbjct: 280 339 Query: 355 VTNTIPLTGVTLPFISYGGSSLLVTMLSMGIMLSIS--RETNRPAKEEVTKSVTTVRQVR 412 Sbjct: 340 399 >BS_ylaO [D] COG0772 Bacterial cell division membrane protein Length = 403 Score = 181 bits (460), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 123/365 (33%), Positives = 183/365 (50%), Gaps = 29/365 (7%) Query: 49 ASEKFGNDPFYFVKRQIIWVVLGSFVMFVTMNIH------YSKFKKWYAPIFMITLVLLL 102 A ++G +F RQ+ ++ G +F+ M + + KF+K + ++ L+ L Sbjct: 36 AVSRYGVSSNFFFMRQLFALIAGG-ALFILMALFPYKALAHQKFQKGILLVSVLALISLF 94 Query: 103 FVAFAERINGAKSWLNIGGLGIQPTEXXXXXXXXXXXXXXXXXGERLRDLRTGYIPVMVI 162 F A+SW IGG+ IQP E + L TG P +V+ Sbjct: 95 --VFGHVAGNAQSWFKIGGMSIQPGEFVKLVVILYLAAVYAKKQSYIDHLLTGVAPPVVM 152 Query: 163 VGIVAGLIMMQPDLGSCLILVATSGLVIYAGGASMKHIMASIXXXXXXXXXXXXXXXXID 222 I+ GLI MQPD G+ +I+ + +I G S K ++ + Sbjct: 153 TLIICGLIAMQPDFGTAMIIGLIATCMILCSGFSGKTLVRLVILGGIVFI---------- 202 Query: 223 SLSPPSETAAVSKDYKKDRISAFINPEADP----EDGGYNILQSLIALGEGGANGSGFGQ 278 L P K + R++ F + E DP G ++ S A+ GG G G G+ Sbjct: 203 -LVSPIIYLNQDKILTEGRLARFESLE-DPFKYANSSGLQVINSYYAISSGGIFGLGLGE 260 Query: 279 SIQKLHYLPYPYTDFIFAVVGEELGFIGTSLFLLAYLYLIWRGILIALRCTDPFGTLVGI 338 SIQK YLP +TDFI AV+ EELG G + +++ +G IA +C DPFG+L+ I Sbjct: 261 SIQKYGYLPESHTDFIMAVIAEELGIFGVLFVIFLLGFVVIKGFYIARKCEDPFGSLLAI 320 Query: 339 GVMGLIAIQAFVNIGGVTNTIPLTGVTLPFISYGGSSLLVTMLSMGIMLSISR----ETN 394 G+ +IAIQ+F+N+GGV+ IP+TGVTLPFISYGGSSL++ + SMGI+ +IS N Sbjct: 321 GISSMIAIQSFINLGGVSGLIPITGVTLPFISYGGSSLVLLLGSMGILANISMFVKYSEN 380 Query: 395 RPAKE 399 + KE Sbjct: 381 KKKKE 385 >HI1137 [D] COG0772 Bacterial cell division membrane protein Length = 394 Score = 178 bits (451), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 111/350 (31%), Positives = 174/350 (49%), Gaps = 22/350 (6%) Query: 50 SEKFGNDPFYFVKRQIIWVVLGSFVMFVTMNIHYSKFKKWYAPIFMITLVLLLFVAF-AE 108 S + NDPFYF KR I+V+L ++++ I S+++KW+A IF+ +++LLL V F Sbjct: 51 SSRLFNDPFYFAKRDAIYVLLSLLTCYISLQISSSQWEKWHAKIFLFSVILLLLVPFIGT 110 Query: 109 RINGAKSWLNIGGLGIQPTEXXXXXXXXXXXXXXXXXGERLRDLRTGYIPVMVIVGIVAG 168 +NGAK W+++G L QP E + +R +++ ++ Sbjct: 111 SVNGAKRWISLGILNFQPAEFAKLALTCFLASYFTRRYDEVRSRHVSIFKPFIVMLVLGC 170 Query: 169 LIMMQPDLGSCLILVATSGLVIYAGGASMKHIMASIXXXXXXXXXXXXXXXXIDSLSPPS 228 +++QPDLGS ++L +++ GA + + I Sbjct: 171 FLLLQPDLGSTVVLFIIMSGMLFIVGAKILQFVGLIALGGILFVWL-------------- 216 Query: 229 ETAAVSKDYKKDRISAFINPEADPEDGGYNILQSLIALGEGGANGSGFGQSIQKLHYLPY 288 ++ Y+ R F+ P +P G+ + SLIA G G G G G SIQKL YLP Sbjct: 217 ---VLTASYRLKRFIGFLEPFKEPYGTGFQLTNSLIAFGRGEITGEGLGNSIQKLDYLPE 273 Query: 289 PYTDFIFAVVGEELGFIGTSLFLLAYLYLIWRGILI---ALRCTDPFGTLVGIGVMGLIA 345 +TDFI A++GEE GFIG + +L LI+R + I +L F +G+ I Sbjct: 274 AHTDFIMAIIGEEFGFIGILIVILLLGLLIFRAMKIGRESLMLEQRFRGFFALGIGFWIF 333 Query: 346 IQAFVNIGGVTNTIPLTGVTLPFISYGGSSLLVTMLSMGIMLSISRETNR 395 Q FVN+G +P G+T P +SYGGSS+++ ++GI+L I E NR Sbjct: 334 FQGFVNLGMALGMLPTKGLTFPLVSYGGSSIIIMSATIGILLRIDHE-NR 382 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.324 0.141 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,202,544 Number of extensions: 984511 Number of successful extensions: 3851 Number of sequences better than 1.0e-05: 84 Number of HSP's gapped: 3678 Number of HSP's successfully gapped: 105 Length of query: 414 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 305 Effective length of database: 25,807,025 Effective search space: 7871142625 Effective search space used: 7871142625 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 117 (49.7 bits)