BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0547.0007.p (393 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value alr4934 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxy... 341 1e-93 DR1711 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxyp... 313 3e-85 BH1613 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxyp... 313 4e-85 PH1043 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxyp... 310 4e-84 slr1653 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxy... 305 9e-83 >alr4934 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Length = 359 Score = 341 bits (874), Expect = 1e-93, Method: Compositional matrix adjust. Identities = 0/390 (0%), Positives = 0/390 (0%), Gaps = 4/390 (1%) Query: 7 RLTLEQLLPDMVKWRRHLHRHPELSYQEKETSAFVAAKLIEFGIEVVRSKAGYGLTGILK 66 L +L P E + A +E G+T + Sbjct: 17 LQRLRELAPASKMVAVVKANAYGHGLLETARTLPDADAFGVARLEEALRLRAGGITKPVL 76 Query: 67 GKSP-GKTVVLRADMDALAITEENGREYASQNHGVMHACGHDGHTSMXXXXXXXXXXXXX 125 L T + E + + Sbjct: 77 LLEGFFDARDLPTISAQHFHTAVHNEEQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQ 136 Query: 126 XLQGELRFLFQPAEEICPGGALGMIAEGVLEG--ADAVYGLHLWTPLPVGKVASAPGPLM 183 R P + A A T + Sbjct: 137 AEAFYHRLTQCKNVR-QPVNIVSHFARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASG 195 Query: 184 ASADEFFIDITGKGGHAGTPHRTVDSIVAAAALVTQLQSIVSRSVDPMRPAVVSVGTIQG 243 + + A + S + + Sbjct: 196 GILLWPQSHFDWVRPGIILYGVSPLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGY 255 Query: 244 GTAQNIVAERCRITGTVRAFDEETRYLIRQRIEEMTAAVAASYGAEAKIDYLMGYPPLVN 303 G + + D R + G A + P Sbjct: 256 GGTWVSERDTRLGVVAMGYGDGYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQ 315 Query: 304 DEAEFQRYFRVAPAALGREVSVERMEKIMPAEDFSYYVKEIPGCFIFVGAGNPDKEAVYP 363 Sbjct: 316 375 Query: 364 HHHSKFDFDEDALLHGAKVLVAMADSCMNE 393 Sbjct: 376 405 >DR1711 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Length = 388 Score = 313 bits (802), Expect = 3e-85, Method: Compositional matrix adjust. Identities = 168/376 (44%), Positives = 220/376 (58%), Gaps = 3/376 (0%) Query: 13 LLPDMVKWRRHLHRHPELSYQEKETSAFVAAKLIEF-GIEVVRSKAGYGLTGILKGKSPG 71 L +V WRRHLH+HPEL +QE +T+ +V +L + G+ + R + +LKG PG Sbjct: 9 LREQVVAWRRHLHQHPELGFQEHQTARYVEEQLRDMPGLHLSRPTE-TSVLAVLKGGKPG 67 Query: 72 KTVVLRADMDALAITEENGREYASQNHGVMHACGHDGHTSMXXXXXXXXXXXXXXLQGEL 131 +TV+LRADMDAL I EE E+ SQ GVMHACGHDGHT+M L GE+ Sbjct: 68 RTVLLRADMDALPIEEEADVEFKSQTPGVMHACGHDGHTAMLLGAAKQLSAQAADLHGEV 127 Query: 132 RFLFQPAEEICPGGALGMIAEGVLEGADAVYGLHLWTPLPVGKVASAPGPLMASADEFFI 191 RF+FQ AEE+ PGGA ++ GV++G D G HL T +PVG V G LMA+ D F I Sbjct: 128 RFIFQHAEEVFPGGAEQLVDAGVMDGVDLAVGTHLMTSVPVGTVVLRDGALMAAPDAFDI 187 Query: 192 DITGKGGHAGTPHRTVDSIVAAAALVTQLQSIVSRSVDPMRPAVVSVGTIQGGTAQNIVA 251 I GKGGH PH+TVD +V AA +V Q+ VSR DP+ P VVSV I GG+A N++ Sbjct: 188 TIQGKGGHGAMPHQTVDPVVIAAQVVMAFQTAVSRLRDPIDPGVVSVTQIHGGSAHNVIP 247 Query: 252 ERCRITGTVRAFDEETRYLIRQRIEEMTAAVAASYGAEAKIDYLMGYPPLVNDEAEFQRY 311 + + GTVR F +E R + R+E + + +YGA Y GY + ND A +R Sbjct: 248 DTVTLGGTVRTFSDELRAQMPGRLETLLRGICEAYGATYTFTYHGGYRSVNNDPATTERL 307 Query: 312 FRVAPAALGREVSVERMEKIMPAEDFSYYVKEIPGCFIFVGAGNPDKEAVYPHHHSKFDF 371 +V L EV+V +M EDFS Y+ PG F+ +GAGN +K PHHH KF Sbjct: 308 RKVVREVL-PEVTVSDGVPLMGGEDFSAYLTRAPGTFVLIGAGNAEKGMTAPHHHPKFMI 366 Query: 372 DEDALLHGAKVLVAMA 387 DE AL G ++ V A Sbjct: 367 DESALEQGVQIYVGAA 382 >BH1613 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Length = 404 Score = 313 bits (801), Expect = 4e-85, Method: Compositional matrix adjust. Identities = 165/375 (44%), Positives = 222/375 (59%), Gaps = 1/375 (0%) Query: 10 LEQLLPDMVKWRRHLHRHPELSYQEKETSAFVAAKLIEFGIEVVRSKAGYGLTGILKGKS 69 ++ ++V RR LH+HPELS++E +T AF+A L + G+ V R G G+ G +KG Sbjct: 9 VDAYFDELVDIRRTLHKHPELSFEEVKTPAFIADYLEKLGVNVRRGVGGRGVVGTIKGGK 68 Query: 70 PGKTVVLRADMDALAITEENGREYASQNHGVMHACGHDGHTSMXXXXXXXXXXXXXXLQG 129 PG TV LRAD DAL I EE G Y S GVMHACGHDGHT+ L+G Sbjct: 69 PGPTVALRADFDALPIQEETGLPYQSTVPGVMHACGHDGHTATLLIIAKILMEHRQQLEG 128 Query: 130 ELRFLFQPAEEICPGGALGMIAEGVLEGADAVYGLHLWTPLPVGKVASAPGPLMASADEF 189 + + Q AEE+ PGGA+ MI +G L+G D +YG HLW +P G++ G MA+AD+F Sbjct: 129 SVVLIHQFAEELAPGGAIAMIEDGCLDGVDVIYGTHLWATMPAGEIGYRSGAFMAAADKF 188 Query: 190 FIDITGKGGHAGTPHRTVDSIVAAAALVTQLQSIVSRSVDPMRPAVVSVGTIQGGTAQNI 249 + I GKGGH PH TVD++ A++V QLQ IVSR+VDP+ AVV+V + Q G N+ Sbjct: 189 AVTIYGKGGHGAAPHETVDAVTVGASVVQQLQQIVSRNVDPLESAVVTVASFQAGGPFNV 248 Query: 250 VAERCRITGTVRAFDEETRYLIRQRIEEMTAAVAASYGAEAKIDYLMGYPPLVNDEAEFQ 309 +A++ + GTVR F E+ + +I +R+E V + GA Y GYP ++N E Sbjct: 249 IADKANLVGTVRTFKEDIQDMIIERMERTIKGVCDAAGATYAFAYDKGYPAVINHPKETD 308 Query: 310 RYFRVAPAALGREVSVERMEKIMPAEDFSYYVKEIPGCFIFVGAGNPDKEAVYPHHHSKF 369 A A +G E V M +M EDF+YY+ +PG F F GAGN D +YPHHH KF Sbjct: 309 DLKNAAVAVVGEE-HVAEMAPVMGGEDFAYYLHHVPGAFFFTGAGNEDAGIIYPHHHPKF 367 Query: 370 DFDEDALLHGAKVLV 384 DE +L AK L+ Sbjct: 368 MIDERSLAIAAKTLL 382 >PH1043 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Length = 387 Score = 310 bits (793), Expect = 4e-84, Method: Compositional matrix adjust. Identities = 166/370 (44%), Positives = 222/370 (60%), Gaps = 13/370 (3%) Query: 17 MVKWRRHLHRHPELSYQEKETSAFVAAKLIEFGIEVVRSKAGYGLTGILKGKSPGKTVVL 76 +V+ RR H +PEL Y+E+ TS V +L + G EVVR+ A G+ GILKGK GKTV L Sbjct: 16 IVEKRRDFHMYPELKYEEERTSKIVEEELKKLGYEVVRT-AKTGVIGILKGKEDGKTVAL 74 Query: 77 RADMDALAITEENGREYASQNHGVMHACGHDGHTSMXXXXXXXXXXXXXXLQGELRFLFQ 136 RADMDAL I EEN Y S+ G MHACGHD HT+M LQG ++ +FQ Sbjct: 75 RADMDALPIQEENDVPYKSRVPGKMHACGHDAHTAMLLGAAKILAEMKDELQGTVKLIFQ 134 Query: 137 PAEEICPGGALG---MIAEGVLEGADAVYGLHLWTPLPVGKVASAPGPLMASADEFFIDI 193 PAEE G LG ++ EG L+ DA++G+H+W LP G + GPL+ASAD F + I Sbjct: 135 PAEE----GGLGAKKIVEEGHLDDVDAIFGIHVWAELPSGIIGIKSGPLLASADAFRVLI 190 Query: 194 TGKGGHAGTPHRTVDSIVAAAALVTQLQSIVSRSVDPMRPAVVSVGTIQGGTAQNIVAER 253 GKGGH PH ++D I A LV Q I+SR VDP++PAV+SV +I+ GT N++ E Sbjct: 191 KGKGGHGAAPHLSIDPIALAVDLVNAYQKIISREVDPLQPAVLSVTSIKAGTTFNVIPES 250 Query: 254 CRITGTVRAFDEETRYLIRQRIEEMTAAVAASYGAEAKIDYLMGY-PPLVNDEAEFQRYF 312 I GT+R FDEE R I +R++E+T A E K + + + PP +N+E + + Sbjct: 251 AEILGTIRTFDEEVRDYIVRRMKEITENFANGMRCEGKFELTIEHIPPTINNE-KLANFA 309 Query: 313 RVAPAALGREVSVERMEKIMPAEDFSYYVKEIPGCFIFVGAGNPDKEAVYPHHHSKFDFD 372 R LG + + M AEDF++Y + PG FIF+G N +K +YPHHH KF+ D Sbjct: 310 RDVLKVLG---EIREPKPTMGAEDFAFYTTKAPGLFIFLGIRNEEKGIIYPHHHPKFNVD 366 Query: 373 EDALLHGAKV 382 ED L GA + Sbjct: 367 EDILWMGAAI 376 >slr1653 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Length = 416 Score = 305 bits (781), Expect = 9e-83, Method: Compositional matrix adjust. Identities = 155/388 (39%), Positives = 225/388 (57%), Gaps = 3/388 (0%) Query: 7 RLTLEQLLPDMVKWRRHLHRHPELSYQEKETSAFVAAKLIEFGIEVVRSKAGYGLTGILK 66 RL ++ L +++WRR H++PEL +QE+ T+A +A L + I A G+ + Sbjct: 29 RLPIQALHGQLIQWRRQFHQYPELGFQEQLTAAHIAETLTKLEIPHTPGIAKTGIMATVD 88 Query: 67 GKSPGKTVVLRADMDALAITEENGREYASQNHGVMHACGHDGHTSMXXXXXXXXXXXXXX 126 PG + +RADMDAL +TEEN +Y S + G MHACGHDGHT++ Sbjct: 89 SGKPGPVLAIRADMDALPVTEENEVDYRSLHPGKMHACGHDGHTAIALGTAQYLAAHRDS 148 Query: 127 LQGELRFLFQPAEEICPGGALGMIAEGVLEG--ADAVYGLHLWTPLPVGKVASAPGPLMA 184 +G+++F FQPAEE PGGA MI GVLE DA+ GLHLW LPVG V PGP+MA Sbjct: 149 FRGQVKFFFQPAEE-GPGGAKPMIEAGVLENPAVDAIVGLHLWNDLPVGTVGIKPGPVMA 207 Query: 185 SADEFFIDITGKGGHAGTPHRTVDSIVAAAALVTQLQSIVSRSVDPMRPAVVSVGTIQGG 244 + + F + G+GGH PH+TVD++V +A +V LQ IV+R+++P++ AVV+VG +Q G Sbjct: 208 AVEHFECQLFGQGGHGAMPHQTVDTLVISAQIVMALQGIVARNLNPLQSAVVTVGQLQSG 267 Query: 245 TAQNIVAERCRITGTVRAFDEETRYLIRQRIEEMTAAVAASYGAEAKIDYLMGYPPLVND 304 TA N++ + GTVR FD QRIEE+ + S+GA + Y YPP+VND Sbjct: 268 TAFNVIPDSAYFRGTVRYFDPSFAGYFAQRIEEIIKGICQSHGANYQFTYENIYPPVVND 327 Query: 305 EAEFQRYFRVAPAALGREVSVERMEKIMPAEDFSYYVKEIPGCFIFVGAGNPDKEAVYPH 364 A L + ++ + + ED S++++ +PGC+ F+G+ N D YPH Sbjct: 328 RRLADLVRSAAADVLLTDDHLQPDYQTLAGEDMSFFLQAVPGCYFFLGSANGDLGLAYPH 387 Query: 365 HHSKFDFDEDALLHGAKVLVAMADSCMN 392 HH +F+FDE L G ++ V + N Sbjct: 388 HHPRFNFDEAVLPVGVELFVRCVERFCN 415 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.136 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 29,121,477 Number of extensions: 1151371 Number of successful extensions: 4938 Number of sequences better than 1.0e-05: 73 Number of HSP's gapped: 4742 Number of HSP's successfully gapped: 73 Length of query: 393 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 285 Effective length of database: 25,922,359 Effective search space: 7387872315 Effective search space used: 7387872315 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)