BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0545.0065.p (424 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MA0181 [GEPR] COG0477 Permeases of the major facilitator superfa... 87 6e-17 TM1021 [GEPR] COG0477 Permeases of the major facilitator superfa... 66 1e-10 SPCC1281.04 [C] COG0667 Predicted oxidoreductases (related to ar... 59 2e-08 SPCC1281.06c_2 [CI] COG5274 Cytochrome b involved in lipid metab... 59 2e-08 >MA0181 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 410 Score = 87.0 bits (214), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 93/407 (22%), Positives = 178/407 (43%), Gaps = 40/407 (9%) Query: 21 GNSMLLPILPQISNELGISAFQVSMIITVYGLIAILMIPIAGYLSDRFGRKKVXXXXXXX 80 G S+L P+LP + L ++ +V ++I+ Y + + + G+ DR RKK+ Sbjct: 30 GGSLLAPVLPDMVEPLNTTSHEVGLLISTYAISTAIFALVIGHFIDRVNRKKILVPCLVI 89 Query: 81 XXXXXXXXXXXXWFMNGVSAYWV-----ILAGRFIQGIGAAGAFPIVIPFIGDLFKDEKE 135 + + G+ +Y+ +L RFIQGIG AG + + I D++KD K Sbjct: 90 ------------YGLTGLVSYFTSDLHSLLILRFIQGIGVAGMMSLPMLVIADVYKDHKS 137 Query: 136 VSKGLGVVETSNTFGKVLSPILGAYLGTVVWYAPFMAIPILCLISFGLVVFLVKKPEAGE 195 + + V + G V +P++G L + W PF+ + ++F ++ FL PE Sbjct: 138 L-YAMNKVSMALAIGSVSAPLIGGGLANLGWNYPFLFYVLSLPLAFVVMFFL---PETRV 193 Query: 196 QPEKKALRRFLGEIKDILHERGRWLYAIFAIGGICMFVTFGVLFYLSETLEAKYNLHGSS 255 Q + + + L + + R LY +F + F+ + VL Y+ L+ + Sbjct: 194 QRDNVSHKGILDGFTAL--KEMRILYTVF-LSFAVFFILYSVLTYVPFMLKNVLSYTAKE 250 Query: 256 KGFXXXXXXXXXXXXSYGSGKIIGQNKA-LMKWLGFGGMVLLTAAMIITGFNKNIYFMVG 314 G + ++ L+ +GF L A+ F +I ++ Sbjct: 251 SGLILAFQGIAIILMVPRVKTLASKHSTILIIAIGFA---LDGLAIFSISFAYSIVTVLL 307 Query: 315 FLSLSGIGIGVALPCMDALITESIEKENRGTITSLYSSMRFIGVALGPPVVSLLMTRGHW 374 + L G G G++ +DA I + E++G + S++++M++IG +L P ++ +++ H+ Sbjct: 308 LILLFGAGFGLSQTAIDAQIVQIAPSESKGGVLSIHNTMKYIGQSLSPILLGVVL--AHF 365 Query: 375 VLFGTMAATGAVG---GLLTLFAVKPSEGGNKEDKPLKSGRVRSNPL 418 L +G+ G L+T K E +E R+R N + Sbjct: 366 DLETVFIVSGSFGLLVALMTYLMKKIFENSGEE-------RIRENKI 405 >TM1021 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 422 Score = 65.9 bits (159), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 52/186 (27%), Positives = 94/186 (50%), Gaps = 14/186 (7%) Query: 14 IPLIMTLGNS---MLLPILPQISNELGISAFQVSMIITVYGLIAILMIPIAGYLSDRFGR 70 I ++M L N+ ++ P + I E I+ Q+ ++ + + +I L+ + GYL+DR+ R Sbjct: 6 ILILMVLLNADQMVMSPNIGAIEQEFNITDAQIGLVASSFTVIGALVSLVWGYLADRYSR 65 Query: 71 KKVXXXXXXXXXXXXXXXXXXXWFMNGVS-AYWVILAGRFIQGIGAAGAFPIVIPFIGDL 129 K + M+ S +Y + R + GIG +FPIV IGD+ Sbjct: 66 KNLLIYSILVGEIPC--------LMSAFSRSYGELFFWRALTGIGVGASFPIVYSMIGDM 117 Query: 130 FKDEKEVSKGLGVVETSNTFGKVLSPILGAYLGTVV-WYAPFMAIPILCLISFGLVVFLV 188 F DE + K + ++ ++ + G VL I+G +LG W PF+A+ + + L +F++ Sbjct: 118 F-DEVKRGKVVALISSAISIGSVLGMIVGGFLGPKYGWRVPFIAVSVPNIFFAVLSIFVL 176 Query: 189 KKPEAG 194 K+P+ G Sbjct: 177 KEPKRG 182 >SPCC1281.04 [C] COG0667 Predicted oxidoreductases (related to aryl-alcohol dehydrogenases) Length = 346 Score = 58.9 bits (141), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 0/366 (0%), Positives = 0/366 (0%), Gaps = 43/366 (11%) Query: 17 IMTLGNSMLLPILPQISNELG-----ISAFQVSMIITVYGLIAILMIPIAGYLSDRFGRK 71 L ++ I S ++ + + + + G + Sbjct: 195 KHDLP--RIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPA 252 Query: 72 KVXXXXXXXXXXXXXXXXXXXWFMNGVSAYWVILAGRFIQGIGAAGAFPIVIPFIGDLFK 131 + + R A + + Sbjct: 253 GARNTLFSRFTRYSG-------EQTQKAVAAYVDIARRHGLDPAQ-MALAFVRRQPFVAS 304 Query: 132 DEKEVSKGLGVVETSNTFGKVLSPILGAYLGTVVWYAPFMAIPILCLIS--FGLVVFLVK 189 Sbjct: 305 TLLG- 363 Query: 190 KPEAGEQPE---KKALRRFLGEIKDILHERGRWLYAIFAIGGICMFVTFGVLFYLSET-L 245 Sbjct: 364 ------------- 410 Query: 246 EAKYNLHGSSKGFXXXXXXXXXXXXSYGSGKIIGQNKAL---MKWLGFGGMVLLTAAMII 302 Sbjct: 411 - 469 Query: 303 TGFNKNIYFMVGFLSLSGIGI----GVALPCMDALITESIEKENRGTITSLYSSMRFIGV 358 Sbjct: 470 529 Query: 359 ALGPPV 364 Sbjct: 530 535 >SPCC1281.06c_2 [CI] COG5274 Cytochrome b involved in lipid metabolism Length = 123 Score = 58.9 bits (141), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 0/366 (0%), Positives = 0/366 (0%), Gaps = 43/366 (11%) Query: 17 IMTLGNSMLLPILPQISNELG-----ISAFQVSMIITVYGLIAILMIPIAGYLSDRFGRK 71 Sbjct: 195 -- 252 Query: 72 KVXXXXXXXXXXXXXXXXXXXWFMNGVSAYWVILAGRFIQGIGAAGAFPIVIPFIGDLFK 131 Sbjct: 253 -------- 304 Query: 132 DEKEVSKGLGVVETSNTFGKVLSPILGAYLGTVVWYAPFMAIPILCLIS--FGLVVFLVK 189 Sbjct: 305 - 363 Query: 190 KPEAGEQPE---KKALRRFLGEIKDILHERGRWLYAIFAIGGICMFVTFGVLFYLSET-L 245 Sbjct: 364 ------------- 410 Query: 246 EAKYNLHGSSKGFXXXXXXXXXXXXSYGSGKIIGQNKAL---MKWLGFGGMVLLTAAMII 302 Sbjct: 411 - 469 Query: 303 TGFNKNIYFMVGFLSLSGIGI----GVALPCMDALITESIEKENRGTITSLYSSMRFIGV 358 Sbjct: 470 529 Query: 359 ALGPPV 364 Sbjct: 530 535 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.325 0.144 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,955,306 Number of extensions: 1212650 Number of successful extensions: 3873 Number of sequences better than 1.0e-05: 39 Number of HSP's gapped: 3874 Number of HSP's successfully gapped: 41 Length of query: 424 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 315 Effective length of database: 25,807,025 Effective search space: 8129212875 Effective search space used: 8129212875 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 117 (49.7 bits)