BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0545.0064.p (621 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2927 [G] COG0366 Glycosidases 527 e-149 lin2231 [G] COG0366 Glycosidases 524 e-148 BS_yvdF [G] COG0366 Glycosidases 516 e-146 L128694 [G] COG0366 Glycosidases 455 e-127 SPy1304 [G] COG0366 Glycosidases 384 e-106 >BH2927 [G] COG0366 Glycosidases Length = 578 Score = 527 bits (1358), Expect = e-149, Method: Compositional matrix adjust. Identities = 273/579 (47%), Positives = 357/579 (61%), Gaps = 18/579 (3%) Query: 25 REALHHVPHSNWAYLYDKDTFHLRVRTGKANVDQVFALTGDKYDW---EHYHEEHRMQKI 81 + ++H P S AY YDK T H+R+RTGK +V +V + GD YDW + + M Sbjct: 2 KATIYHRPKSEDAYAYDKSTMHIRLRTGKNHVRKVNLIYGDPYDWTKNQWVTSKKEMTPT 61 Query: 82 ASDQFFDYWETAVKPENKRFSYAFRMQAGDETLWMTETGFFTELPAPPEGYYERPYIHEA 141 SD D+W V P +R Y F + ET++ E+GF Y+ P+++ A Sbjct: 62 GSDAQHDFWFVEVTPPYRRLRYGFEIITKTETIYYGESGFLDTPSKDTSDYFCFPFMNPA 121 Query: 142 DLFKAPEWAKSAVFYQIMPDRFANGDPGKDPVNVAAWGDT-PTAESFFGGDLQGIMDHLD 200 DLF PEW K V+YQI P+RFANGDP ++P WG PT + FGGDLQGI+DHLD Sbjct: 122 DLFSPPEWVKDTVWYQIFPERFANGDPSRNPEGTLPWGSAAPTPTNHFGGDLQGIIDHLD 181 Query: 201 YLARLGITAIYLTPIFEAPSNHKYDTTDYRKVDPNFGDVELLKKLVTTAHAKGIRVVLDA 260 YL LGI IYLTPIFEA SNHKYDT DY KVDP+FGD K+LV H +GIR++LDA Sbjct: 182 YLQELGINGIYLTPIFEASSNHKYDTIDYLKVDPHFGDKTTFKRLVDECHKRGIRIMLDA 241 Query: 261 VFNHISAQSPQFQSVIEHGEQSEYAGWFHIHNFPVKVTDGKPNYDAFGFFADMPKLNTTN 320 VFNH P FQ V+++ + S+Y+ WFH+ +FPV+ + PNYD F F MPK NT N Sbjct: 242 VFNHAGFYFPPFQDVLKNQQHSKYSDWFHLRDFPVRAEE-PPNYDTFAFTPFMPKFNTEN 300 Query: 321 PEARKYLLDTAGYWLEEVGIDGWRLDVANEIDHTFWRDFRKLVKDINPEAFIIGEVWSDS 380 E R+YLL+ A YW++E IDGWRLDVANE+DH FWR+FRK VK + P+ +I+GEVW +S Sbjct: 301 EEVREYLLNVATYWIKEFDIDGWRLDVANEVDHAFWREFRKRVKALKPDLYILGEVWHNS 360 Query: 381 MRWLNGDQFDSVMNYPLTSRLIEYLNNGDINAGRFGGQLGNLLMRYPQQANEVLFNLLAS 440 + WL GDQFD+VMNYPLT+ +I++ I RF + L YP +V FNLL S Sbjct: 361 LPWLAGDQFDAVMNYPLTNAIIDFAGKQKIGQQRFEHVITQLTHMYPIHVQQVAFNLLGS 420 Query: 441 HDTPRILTQLGGDPGKLKLAAAFLFTFTGTPCIFYGDEIGLEGEGDPDCRKCMIWEGERQ 500 HDTPRILTQ D ++KL LF+ GTPCI+YGDEIG+ G DP CRKCM+WE E Q Sbjct: 421 HDTPRILTQTDNDVARVKLQFLLLFSQRGTPCIYYGDEIGMTGGQDPGCRKCMVWEEEEQ 480 Query: 501 NRDLFESYQSLIRLRKAFPALQT-GRFRILSADPQQRAFVYERVDENAHFVISLNPSEQP 559 N++L + LI+LRK A T G + D +Y++ EN + +N ++QP Sbjct: 481 NQELLSFVKRLIQLRKQEKAFGTEGDLTFVEND--SSLLIYQKTYENQQLLFVINSTDQP 538 Query: 560 ATLRLPLE---DRFQDALNGEK-------LIPSDGQMVL 588 +++LP+ D L GE +IP+ MVL Sbjct: 539 DSVQLPVSWTNRNANDLLTGETFSLATEIVIPAKSAMVL 577 >lin2231 [G] COG0366 Glycosidases Length = 591 Score = 524 bits (1350), Expect = e-148, Method: Compositional matrix adjust. Identities = 267/589 (45%), Positives = 366/589 (62%), Gaps = 25/589 (4%) Query: 23 IQREALHHVPHSNWAYLYDKDTFHLRVRTGKANVDQVFALTGDKYDWEH---YHEEHRMQ 79 +++ ++H P S++AY YD T H+R+RT + ++ +V + D Y W++ E + M+ Sbjct: 1 MEKAGIYHQPASSYAYSYDAKTLHIRIRTKRLDISKVTLIAADPYLWKNEKWQSESYSMR 60 Query: 80 KIASDQFFDYWETAVKPENKRFSYAFRMQAGD-ETLWMTETGFF--TELP-APPEGYYER 135 KIA + DYW A+ PE++R Y F + + ET + GFF TE A + Y++ Sbjct: 61 KIAETEEHDYWFFAITPEHRRLQYGFLLTDTEGETTFYGGRGFFEPTEANLATMDYYFKF 120 Query: 136 PYIHEADLFKAPEWAKSAVFYQIMPDRFANGDPGKDPVNVAAWGDT-PTAESFFGGDLQG 194 P+IH D F+APEW + ++YQI P+RFANG+P P N WG P FFGGDL+G Sbjct: 121 PFIHAVDTFEAPEWVGNTIWYQIFPERFANGNPAISPENALPWGSKDPNTTDFFGGDLEG 180 Query: 195 IMDHLDYLARLGITAIYLTPIFEAPSNHKYDTTDYRKVDPNFGDVELLKKLVTTAHAKGI 254 I+ HLDYLA LGI +YLTP+FEAP+NHKYDT DY+K+DP+FGD E +KLV AH +GI Sbjct: 181 IIQHLDYLAELGINGVYLTPVFEAPTNHKYDTIDYKKIDPHFGDKETFRKLVQEAHKRGI 240 Query: 255 RVVLDAVFNHISAQSPQFQSVIEHGEQSEYAGWFHIHNFPVKVTD-----GKP--NYDAF 307 R++LDAVFNHI S ++Q V+E E+S Y WFHIH+FPV+ + G+P +YD F Sbjct: 241 RIMLDAVFNHIGDTSLEWQDVVEKEEKSAYRDWFHIHSFPVRQNENGNIEGEPTLSYDTF 300 Query: 308 GFFADMPKLNTTNPEARKYLLDTAGYWLEEVGIDGWRLDVANEIDHTFWRDFRKLVKDIN 367 F MPKLNT NPE +KYLLD A YW+ E IDGWRLDVANE+DH FW++F+K V+ Sbjct: 301 AFTTHMPKLNTANPEVQKYLLDIATYWIREFDIDGWRLDVANEVDHAFWKEFKKAVQAEK 360 Query: 368 PEAFIIGEVWSDSMRWLNGDQFDSVMNYPLTSRLIEYLNNGDINAGRFGGQLGNLLMRYP 427 + +I+GE+W DS WL GD+F +VMNYP T +IE I + + MRYP Sbjct: 361 EDIYILGEIWHDSWIWLLGDEFHAVMNYPFTQTIIENFIEEKITPEQMVSGINEQYMRYP 420 Query: 428 QQANEVLFNLLASHDTPRILTQLGGDPGKLKLAAAFLFTFTGTPCIFYGDEIGLEGEGDP 487 Q NEV+FN+L SHDT RILT+ D K+K A AF+F TG+PCI+YG EIG++G DP Sbjct: 421 NQVNEVMFNMLDSHDTARILTRANNDEDKVKQALAFMFAHTGSPCIYYGTEIGMDGGNDP 480 Query: 488 DCRKCMIWEGERQNRDLFESYQSLIRLRKAFPALQT-GRFRILSADPQQ--RAFVYERVD 544 CRKCM W+ +QN+D+ + LI LRK A+ T G L A + AF E Sbjct: 481 GCRKCMEWDETKQNQDMLAFTKQLIALRKDNQAIITSGELNWLGASSETGITAFSKELNG 540 Query: 545 ENAHFVISLNPSEQPATLRLPLEDRFQDALNGEK-----LIPSDGQMVL 588 E HF+ N +++ + E+ D N ++P+ G +V+ Sbjct: 541 EKLHFL--FNQAKEDKDFTISFENTATDIWNNRAVTENLVVPAKGFLVI 587 >BS_yvdF [G] COG0366 Glycosidases Length = 589 Score = 516 bits (1330), Expect = e-146, Method: Compositional matrix adjust. Identities = 257/508 (50%), Positives = 332/508 (65%), Gaps = 11/508 (2%) Query: 23 IQREALHHVPHSNWAYLYDKDTFHLRVRTGKANVDQVFALTGDKYDW---EHYHEEHRMQ 79 ++ A+HH P S AY YD T H+++RT K + D + + GD Y++ + E M+ Sbjct: 2 MEYAAIHHQPFSTDAYSYDGRTVHIKIRTKKGDADHIRFIWGDPYEYNDGKWSANEQPMR 61 Query: 80 KIASDQFFDYWETAVKPENKRFSYAFRMQAGDETLWMTETGF--FTELPAPPEGYYER-P 136 KIA+ + DYW V P +R YAF + E ++ +G + E YY + P Sbjct: 62 KIAATEMHDYWFAEVVPPFRRLQYAFVVTDDHEDIFFGSSGVCPYNEKTLETIHYYFKFP 121 Query: 137 YIHEADLFKAPEWAKSAVFYQIMPDRFANGDPGKDPVNVAAWGDT-PTAESFFGGDLQGI 195 ++HEAD F+APEW KS V+YQI P+RFANG P N WG P FFGGDLQGI Sbjct: 122 FVHEADTFQAPEWVKSTVWYQIFPERFANGREDLSPKNALPWGSKDPDVNDFFGGDLQGI 181 Query: 196 MDHLDYLARLGITAIYLTPIFEAPSNHKYDTTDYRKVDPNFGDVELLKKLVTTAHAKGIR 255 +D LDYL LG+ IYLTPIF APSNHKYDT DY +DP+FGD EL + LV+ H +G+R Sbjct: 182 VDKLDYLEDLGVNGIYLTPIFSAPSNHKYDTLDYFSIDPHFGDPELFRTLVSQLHQRGMR 241 Query: 256 VVLDAVFNHISAQSPQFQSVIEHGEQSEYAGWFHIHNFPVKVTDGKPNYDAFGFFADMPK 315 ++LDAVFNHI + SPQ+Q V+++G+QS Y WFHIH+FPV TD NYD F F ADMPK Sbjct: 242 IMLDAVFNHIGSASPQWQDVVKNGDQSRYKDWFHIHSFPV--TDD--NYDRFAFTADMPK 297 Query: 316 LNTTNPEARKYLLDTAGYWLEEVGIDGWRLDVANEIDHTFWRDFRKLVKDINPEAFIIGE 375 LNT NPE +KYLLD A YW+ E IDGWRLDVANE+DH FW+ FR+ V P+ +I+GE Sbjct: 298 LNTANPEVQKYLLDIALYWIREFDIDGWRLDVANEVDHVFWKTFRQAVSTEKPDVYILGE 357 Query: 376 VWSDSMRWLNGDQFDSVMNYPLTSRLIEYLNNGDINAGRFGGQLGNLLMRYPQQANEVLF 435 +W + WL GD+F + MNYP T +IEY + I+A R ++ LM +QANEV+F Sbjct: 358 IWHSAEPWLRGDEFHAAMNYPFTEPMIEYFADQTISASRMAHRVNAHLMNGMKQANEVMF 417 Query: 436 NLLASHDTPRILTQLGGDPGKLKLAAAFLFTFTGTPCIFYGDEIGLEGEGDPDCRKCMIW 495 NLL SHDT R+LT+ D K + AF+F TG+PCI+YG EIGL GE DP CRKCM+W Sbjct: 418 NLLDSHDTKRLLTRCRNDEKKARALLAFMFAQTGSPCIYYGTEIGLNGENDPLCRKCMVW 477 Query: 496 EGERQNRDLFESYQSLIRLRKAFPALQT 523 E E+QN+D+ + + LI LRK L T Sbjct: 478 EKEKQNQDMLQFMKRLIALRKQENTLLT 505 >L128694 [G] COG0366 Glycosidases Length = 584 Score = 455 bits (1170), Expect = e-127, Method: Compositional matrix adjust. Identities = 235/557 (42%), Positives = 321/557 (57%), Gaps = 23/557 (4%) Query: 23 IQREALHHVPHSNWAYLYDKDTFHLRVRTGKANVDQVFALTGDKYDWEHY---------H 73 + + A++H P S +AYLY K+ H+R++T K++V++V GD Y WE Sbjct: 1 MNKAAIYHRPESEFAYLYAKNLMHVRLQTQKSDVEKVELFYGDPYSWEGLDNPETQYWAS 60 Query: 74 EEHRMQKIASDQFFDYWETAVKPENKRFSYAFRMQAGD-ETLWMTETGFF---TELPAPP 129 ++ M+K S DY+E + KR Y F + D E + T+ G F L Sbjct: 61 QKVEMKKYLSTNISDYFEAEITVATKRVDYLFVITGLDGEKVVYTDQGMFDYDESLVTKK 120 Query: 130 EGYYERPYIHEADLFKAPEWAKSAVFYQIMPDRFANGDPGKDPVNVAAWG--DTPTAESF 187 G + PY HE+D FKAPEW K V+YQI P+RFANGD DP AW D P + F Sbjct: 121 YGAFRLPYFHESDRFKAPEWVKETVWYQIFPERFANGDTSNDPEGTKAWNPQDVPDRQDF 180 Query: 188 FGGDLQGIMDHLDYLARLGITAIYLTPIFEAPSNHKYDTTDYRKVDPNFGDVELLKKLVT 247 +GGDLQG++DHL++L LG+ IY TPIF+A SNHKYDT DY ++D FGD EL K LV Sbjct: 181 YGGDLQGVIDHLEHLTELGVNGIYFTPIFQAFSNHKYDTEDYMEIDKQFGDKELFKTLVK 240 Query: 248 TAHAKGIRVVLDAVFNHISAQSPQFQSVIEHGEQSEYAGWFHIHNFPVKVTDG------- 300 AHA+GI+V+LDAVFNHI S Q+Q V+++ E+S++A WFH++++P T Sbjct: 241 EAHARGIKVMLDAVFNHIGDTSAQWQDVLKNQEKSKFADWFHVNSWPATYTPTDDFENAE 300 Query: 301 KPNYDAFGFFADMPKLNTTNPEARKYLLDTAGYWLEEVGIDGWRLDVANEIDHTFWRDFR 360 YD F F MPKLNT NPE YLL A YW+ E ID WRLDVA+E+DHTFW+ FR Sbjct: 301 NATYDTFAFTPHMPKLNTANPEVEAYLLKVADYWISEFDIDAWRLDVADEVDHTFWKKFR 360 Query: 361 KLVKDINPEAFIIGEVWSDSMRWLNGDQFDSVMNYPLTSRLIEYLNNGDINAGRFGGQLG 420 + +I+GEVW + WL GD+F +VMNY T + + L I+ + + Sbjct: 361 TTCDAAKDDFYILGEVWHSAQPWLVGDEFSAVMNYAYTDAIKDGLITKKISLEQMVSNIN 420 Query: 421 NLLMRYPQQANEVLFNLLASHDTPRILTQLGGDPGKLKLAAAFLFTFTGTPCIFYGDEIG 480 L Y +Q N+++FN+L SHDTPRILT + +KL F F G PCI+YGDE Sbjct: 421 TQLTLYRKQVNQMMFNVLDSHDTPRILTVAKNNKNLMKLVETFTFMQPGVPCIYYGDEYA 480 Query: 481 LEGEGDPDCRKCMIWEGERQNRDLFESYQSLIRLRKAFPA-LQTGRFRILSADPQQRAFV 539 + G DP+CRK M WE + Q+ ++F+ +++LI LRK + L D + Sbjct: 481 VTGGMDPECRKVMPWEEKDQDLEMFDFFKNLIALRKQYQGILSDSEVEWKIVDSENGIVK 540 Query: 540 YERVDENAHFVISLNPS 556 +ER A F I P+ Sbjct: 541 FERGSLKAIFNIGEKPA 557 >SPy1304 [G] COG0366 Glycosidases Length = 567 Score = 384 bits (986), Expect = e-106, Method: Compositional matrix adjust. Identities = 207/547 (37%), Positives = 308/547 (56%), Gaps = 22/547 (4%) Query: 27 ALHHVPHSNWAYLYDKDTFHLRVRTGKANVDQVFALT-GDKYDWEHYHEEHRMQKIASDQ 85 + H+P S + + + +R+R K + L G KY + + EE M +D Sbjct: 5 GMLHIPDSRYCFAISQKELVIRLRVAKEDRGITVNLVHGPKYGYHEFQEELEMSVAYTDH 64 Query: 86 FFDYWETAVKPENKRFSYAFRMQAGDETLWMTETGFFT--ELPAPPEGYYERPYIHEADL 143 Y+ET + ++ R +Y F+++ +T + +E G T + +++ PYI+ D+ Sbjct: 65 THAYYETTLTLDDVRLAYIFKIEEEGQTYYFSEDGLTTSYDFSNGFYNFFQMPYINAVDV 124 Query: 144 FKAPEWAKSAVFYQIMPDRFANGD--PGKDPVNVAAWGDTPTAESFFGGDLQGIMDHLDY 201 + W K AVFYQI DRF GD G VN+ W + PT +SF GGDL+GI + LDY Sbjct: 125 HQVIPWTKDAVFYQIFVDRFNRGDWEKGDSYVNMG-WLEKPTPKSFAGGDLKGITEKLDY 183 Query: 202 LARLGITAIYLTPIFEAPSNHKYDTTDYRKVDPNFGDVELLKKLVTTAHAKGIRVVLDAV 261 L LGIT IYLTPIF++ SNHKYD +DY +DP FG L++L+ AH GI+++LDAV Sbjct: 184 LKDLGITVIYLTPIFQSISNHKYDISDYYAIDPQFGTKYDLQELIDLAHKMGIKIILDAV 243 Query: 262 FNHISAQSPQFQSVIEHGEQSEYAGWFHIHNFPVKVTDGKP-----NYDAFGFFADMPKL 316 FNH S+ + +FQ V+ +G++S++ WF H D P NY+ F MPK Sbjct: 244 FNHASSDAVEFQDVLRYGKESKFFDWFMTH-------DEHPSMDLVNYETFAGCNYMPKW 296 Query: 317 NTTNPEARKYLLDTAGYWLEEVGIDGWRLDVANEIDHTFWRDFRKLVKDINPEAFIIGEV 376 NT+N + + YL++ YW++E IDGWRLDV++E+ H FWR FR+ VK +A +IGE Sbjct: 297 NTSNRDVQDYLIEIGRYWIKEFCIDGWRLDVSDEVSHDFWRRFRQAVKAEKADAILIGEN 356 Query: 377 WSDSMRWLNGDQFDSVMNYPLTSRLIEYLNNGDINAGRFGGQLGNLLMRYPQQANEVLFN 436 W D+ +L GDQ+D +MNY T ++Y + + + +L N+LMR Q N + N Sbjct: 357 WHDAYPYLAGDQYDGIMNYAFTKACLDYFAFETMTSKQLAEKLSNILMRNTWQVNRMNLN 416 Query: 437 LLASHDTPRILTQLGGDPGKLKLAAAFLFTFTGTPCIFYGDEIGLEGEGDPDCRKCMIWE 496 LL SHDT R TQ+ G KL A A LF F G PC++YG E+ +EG DPD R+ W+ Sbjct: 417 LLDSHDTHRFFTQVKGSKDKLLAALALLFIFMGIPCLYYGTEVAMEGGYDPDSRRGFPWD 476 Query: 497 GERQNRDLFESYQSLIRLRKAFPALQTGRFRILSADPQQRAFVYERVDENAHFVISLNPS 556 +++D F +++I LR+ PALQ G +S D + + +R + LN Sbjct: 477 DAERDKDFFSKVKAIIALRQQ-PALQEGA---ISIDYDEDCLILKRSLGEQSISLVLNNG 532 Query: 557 EQPATLR 563 E +++ Sbjct: 533 ESAYSIK 539 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.138 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 51,764,333 Number of extensions: 2375560 Number of successful extensions: 5394 Number of sequences better than 1.0e-05: 115 Number of HSP's gapped: 5088 Number of HSP's successfully gapped: 120 Length of query: 621 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 509 Effective length of database: 25,461,023 Effective search space: 12959660707 Effective search space used: 12959660707 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 119 (50.4 bits)