BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0545.0049.p (278 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value VC2021 [IQR] COG1028 Dehydrogenases with different specificities... 96 9e-20 fabG [IQR] COG1028 Dehydrogenases with different specificities (... 95 1e-19 ZfabG [IQR] COG1028 Dehydrogenases with different specificities ... 95 1e-19 BS_fabG [IQR] COG1028 Dehydrogenases with different specificitie... 95 1e-19 ZfabG [IQR] COG1028 Dehydrogenases with different specificities ... 95 1e-19 >VC2021 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 248 Score = 95.5 bits (236), Expect = 9e-20, Method: Compositional matrix adjust. Identities = 75/257 (29%), Positives = 118/257 (45%), Gaps = 22/257 (8%) Query: 23 LHEFEGKRVLVTGGTRGMGEAIAKRLAASGATVLTT------ARTLPADLQEPGLFVQAD 76 EGK LVTG +RG+G+AIA+ LA GA V+ T A+ + L + G + + Sbjct: 4 FMNLEGKVALVTGASRGIGKAIAELLAERGAKVIGTATSESGAQAISDYLGDNGKGMALN 63 Query: 77 IATPEGVEQVIRGVKEVLGGIDILVNNVGGSSTPPGGALVLTDEDXXXXXXXXXXXXVRL 136 + PE +E V++ + + GG+DILVNN G T + + +E+ RL Sbjct: 64 VTNPESIEAVLKAITDEFGGVDILVNNAG--ITRDNLLMRMKEEEWSDIMETNLTSIFRL 121 Query: 137 DRGLLPMMLEQGYGAIVHISSIQRRLPLYEXXXXXXXXXXXXXXXXXGLSNEFSPRGIRI 196 + +L M+++ G I+++ S+ + ++ E + RG+ + Sbjct: 122 SKAVLRGMMKKRQGRIINVGSVVGTMG-NAGQANYAAAKAGVIGFTKSMAREVASRGVTV 180 Query: 197 NTVAPGFIQTQAAEAMIDRIVQAAGSREAALQQLMDSXXXXXXXXXXXXEEVGELVAFLV 256 NTVAPGFI+T +A+ D R A L Q+ E+ VAFL Sbjct: 181 NTVAPGFIETDMTKALNDE------QRTATLAQV-------PAGRLGDPREIASAVAFLA 227 Query: 257 SDRAASITGSEYVIDGG 273 S AA ITG ++GG Sbjct: 228 SPEAAYITGETLHVNGG 244 >fabG [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 244 Score = 94.7 bits (234), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 77/255 (30%), Positives = 114/255 (44%), Gaps = 22/255 (8%) Query: 25 EFEGKRVLVTGGTRGMGEAIAKRLAASGATVLTT------ARTLPADLQEPGLFVQADIA 78 FEGK LVTG +RG+G AIA+ LAA GA V+ T A+ + L G + ++ Sbjct: 2 NFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQAISDYLGANGKGLMLNVT 61 Query: 79 TPEGVEQVIRGVKEVLGGIDILVNNVGGSSTPPGGALVLTDEDXXXXXXXXXXXXVRLDR 138 P +E V+ ++ G +DILVNN G T + + DE+ RL + Sbjct: 62 DPASIESVLEKIRAEFGEVDILVNNAG--ITRDNLLMRMKDEEWNDIIETNLSSVFRLSK 119 Query: 139 GLLPMMLEQGYGAIVHISSIQRRLPLYEXXXXXXXXXXXXXXXXXGLSNEFSPRGIRINT 198 ++ M+++ +G I+ I S+ + L+ E + RGI +N Sbjct: 120 AVMRAMMKKRHGRIITIGSVVGTMG-NGGQANYAAAKAGLIGFSKSLAREVASRGITVNV 178 Query: 199 VAPGFIQTQAAEAMIDRIVQAAGSREAALQQLMDSXXXXXXXXXXXXEEVGELVAFLVSD 258 VAPGFI+T A+ D R L Q+ +E+ VAFL SD Sbjct: 179 VAPGFIETDMTRALSDD------QRAGILAQV-------PAGRLGGAQEIANAVAFLASD 225 Query: 259 RAASITGSEYVIDGG 273 AA ITG ++GG Sbjct: 226 EAAYITGETLHVNGG 240 >ZfabG [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 244 Score = 94.7 bits (234), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 77/255 (30%), Positives = 114/255 (44%), Gaps = 22/255 (8%) Query: 25 EFEGKRVLVTGGTRGMGEAIAKRLAASGATVLTT------ARTLPADLQEPGLFVQADIA 78 FEGK LVTG +RG+G AIA+ LAA GA V+ T A+ + L G + ++ Sbjct: 2 NFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQAISDYLGANGKGLMLNVT 61 Query: 79 TPEGVEQVIRGVKEVLGGIDILVNNVGGSSTPPGGALVLTDEDXXXXXXXXXXXXVRLDR 138 P +E V+ ++ G +DILVNN G T + + DE+ RL + Sbjct: 62 DPASIESVLEKIRAEFGEVDILVNNAG--ITRDNLLMRMKDEEWNDIIETNLSSVFRLSK 119 Query: 139 GLLPMMLEQGYGAIVHISSIQRRLPLYEXXXXXXXXXXXXXXXXXGLSNEFSPRGIRINT 198 ++ M+++ +G I+ I S+ + L+ E + RGI +N Sbjct: 120 AVMRAMMKKRHGRIITIGSVVGTMG-NGGQANYAAAKAGLIGFSKSLAREVASRGITVNV 178 Query: 199 VAPGFIQTQAAEAMIDRIVQAAGSREAALQQLMDSXXXXXXXXXXXXEEVGELVAFLVSD 258 VAPGFI+T A+ D R L Q+ +E+ VAFL SD Sbjct: 179 VAPGFIETDMTRALSDD------QRAGILVQV-------PAGRLGGAQEIANAVAFLASD 225 Query: 259 RAASITGSEYVIDGG 273 AA ITG ++GG Sbjct: 226 EAAYITGETLHVNGG 240 >BS_fabG [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 246 Score = 94.7 bits (234), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 17/255 (6%), Positives = 49/255 (19%), Gaps = 22/255 (8%) Query: 25 EFEGKRVLVTGGTRGMGEAIAKRLAASGATVLTT------ARTLPADLQEPGLFVQADIA 78 + ++ + V+ A + +++ G A A Sbjct: 2 LNDKTAIVTGASRGIGRSIALDLAKSGANVVVNYSGNEAKANEVVDEIKSMGRKAIAVKA 61 Query: 79 TPEGVEQVIRGVKEVLGGIDILVNNVGGSSTPPGGALVLTDEDXXXXXXXXXXXXVRLDR 138 E V +KE L + V + ++ + Sbjct: 62 DVSNPEDVQNMIKETLSVFSTIDILVN--NAGITRDNLIMRMKEDEWDDVININLKGVFN 119 Query: 139 GLLPMMLEQGYGAIVHISSIQRRLPLYEXXXXXXXXXXXXXXXXXGLSNEFSPRGIRINT 198 + + I ++ + + R T Sbjct: 120 CTKAVTRQMMKQRSGRIINVSSIVG-VSGNPGQANYVAAKAGVIGLTKSSAKELASRNIT 178 Query: 199 VAPGFIQTQAAEAMIDRIVQAAGSREAALQQLMDSXXXXXXXXXXXXEEVGELVAFLVSD 258 V + + +++ + L E ++ + + Sbjct: 179 VNAIAPGFISTDMTDKL------AKDVQDEML-------KQIPLARFGEPSDVSSVVTFL 225 Query: 259 RAASITGSEYVIDGG 273 + Sbjct: 226 ASEGARYMTGQTLHI 240 Score = 94.7 bits (234), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 78/260 (30%), Positives = 114/260 (43%), Gaps = 32/260 (12%) Query: 25 EFEGKRVLVTGGTRGMGEAIAKRLAASGATVLTT------ARTLPADLQEPGLFVQADIA 78 FEGK LVTG +RG+G AIA+ LAA GA V+ T A+ + L G + ++ Sbjct: 2 NFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQAISDYLGANGKGLMLNVT 61 Query: 79 TPEGVEQVIRGVKEVLGGIDILVNNVGGSSTPPGGALVLTDEDXXXXXXXXXXXXVRLDR 138 P +E V+ ++ G +DILVNN G T + + DE+ RL + Sbjct: 62 DPASIESVLEKIRAEFGEVDILVNNAG--ITRDNLLMRMKDEEWNDIIETNLSSVFRLSK 119 Query: 139 GLLPMMLEQGYGAIVHISSIQRRLPLYEXXXXXXXXXXXXXXXXXGLSNEFSPRGIRINT 198 ++ M+++ +G I+ I S+ + L+ E + RGI +N Sbjct: 120 AVMRAMMKKRHGRIITIGSVVGTMG-NGGQANYAAAKAGLIGFSKSLAREVASRGITVNV 178 Query: 199 VAPGFIQTQAAEAMIDR-----IVQAAGSREAALQQLMDSXXXXXXXXXXXXEEVGELVA 253 VAPGFI+T A+ D +VQ R Q E+ VA Sbjct: 179 VAPGFIETDMTRALSDDQRAGILVQVPAGRLGGAQ------------------EIANAVA 220 Query: 254 FLVSDRAASITGSEYVIDGG 273 FL SD AA ITG ++GG Sbjct: 221 FLASDEAAYITGETLHVNGG 240 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.135 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 16,903,784 Number of extensions: 574302 Number of successful extensions: 2689 Number of sequences better than 1.0e-05: 185 Number of HSP's gapped: 2463 Number of HSP's successfully gapped: 189 Length of query: 278 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 173 Effective length of database: 26,268,361 Effective search space: 4544426453 Effective search space used: 4544426453 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 115 (48.9 bits)