BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0545.0035.p (287 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value yeaE [R] COG0656 Aldo/keto reductases, related to diketogulonate... 338 6e-93 Z2821 [R] COG0656 Aldo/keto reductases, related to diketogulonat... 336 2e-92 ECs2490 [R] COG0656 Aldo/keto reductases, related to diketogulon... 336 2e-92 STM1288 [R] COG0656 Aldo/keto reductases, related to diketogulon... 333 2e-91 AGc3682 [R] COG0656 Aldo/keto reductases, related to diketogulon... 290 2e-78 >yeaE [R] COG0656 Aldo/keto reductases, related to diketogulonate reductase Length = 346 Score = 338 bits (867), Expect = 6e-93, Method: Compositional matrix adjust. Identities = 12/279 (4%), Positives = 40/279 (14%) Query: 9 ITLKDGTAVTKMGQGTWNMGDSTATRDEEIAALRLGVELGMNLIDTAEMYGDGRSEEMVG 68 I T ++ + + + + + + Sbjct: 6 IPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLT 65 Query: 69 EAIRGIRDQVFLVSKVHPQNAGLDRITRSCEESLKRLGTDHLDLYLLHWRGKVPLEETVK 128 E G + + + +R+ ++ ++ + +T Sbjct: 66 ETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDY 125 Query: 129 GMEQLVASGKIARWGVSNLDTADMKELLRIPGGDRCAVNQVLYHLGSRGIEHELLPWQRT 188 V + L + + D G Sbjct: 126 LDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFG 185 Query: 189 HKIPVMAYSPLAQAGKLRKGVTENETVREIAEKHGATPLQIMLAWSIREGDIIAIPKASS 248 + + + R + Sbjct: 186 VMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKY 245 Query: 249 RKHVTENAEAAKIRLSEEECWKLDDAFPQPAWKVPLDMI 287 + A+ Sbjct: 246 LNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRH 284 >Z2821 [R] COG0656 Aldo/keto reductases, related to diketogulonate reductase Length = 346 Score = 336 bits (862), Expect = 2e-92, Method: Compositional matrix adjust. Identities = 12/279 (4%), Positives = 40/279 (14%) Query: 9 ITLKDGTAVTKMGQGTWNMGDSTATRDEEIAALRLGVELGMNLIDTAEMYGDGRSEEMVG 68 I T ++ + + + + + + Sbjct: 6 IPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLT 65 Query: 69 EAIRGIRDQVFLVSKVHPQNAGLDRITRSCEESLKRLGTDHLDLYLLHWRGKVPLEETVK 128 E G + + + +R+ ++ ++ + +T Sbjct: 66 ETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDY 125 Query: 129 GMEQLVASGKIARWGVSNLDTADMKELLRIPGGDRCAVNQVLYHLGSRGIEHELLPWQRT 188 V + L + + D G Sbjct: 126 LDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFG 185 Query: 189 HKIPVMAYSPLAQAGKLRKGVTENETVREIAEKHGATPLQIMLAWSIREGDIIAIPKASS 248 + + + R + Sbjct: 186 VMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKY 245 Query: 249 RKHVTENAEAAKIRLSEEECWKLDDAFPQPAWKVPLDMI 287 + A+ Sbjct: 246 LNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRH 284 >ECs2490 [R] COG0656 Aldo/keto reductases, related to diketogulonate reductase Length = 346 Score = 336 bits (862), Expect = 2e-92, Method: Compositional matrix adjust. Identities = 12/279 (4%), Positives = 40/279 (14%) Query: 9 ITLKDGTAVTKMGQGTWNMGDSTATRDEEIAALRLGVELGMNLIDTAEMYGDGRSEEMVG 68 I T ++ + + + + + + Sbjct: 6 IPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLT 65 Query: 69 EAIRGIRDQVFLVSKVHPQNAGLDRITRSCEESLKRLGTDHLDLYLLHWRGKVPLEETVK 128 E G + + + +R+ ++ ++ + +T Sbjct: 66 ETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDY 125 Query: 129 GMEQLVASGKIARWGVSNLDTADMKELLRIPGGDRCAVNQVLYHLGSRGIEHELLPWQRT 188 V + L + + D G Sbjct: 126 LDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFG 185 Query: 189 HKIPVMAYSPLAQAGKLRKGVTENETVREIAEKHGATPLQIMLAWSIREGDIIAIPKASS 248 + + + R + Sbjct: 186 VMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKY 245 Query: 249 RKHVTENAEAAKIRLSEEECWKLDDAFPQPAWKVPLDMI 287 + A+ Sbjct: 246 LNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRH 284 >STM1288 [R] COG0656 Aldo/keto reductases, related to diketogulonate reductase Length = 346 Score = 333 bits (855), Expect = 2e-91, Method: Compositional matrix adjust. Identities = 14/286 (4%), Positives = 55/286 (19%), Gaps = 6/286 (2%) Query: 2 NTNISGTITLKDGTAVTKMGQGTWNMGDSTATRDEEIAALRLGVELGMNLIDTAEMYGDG 61 + S ++ +G T+ +S A ++ +++ + + Sbjct: 6 IPHSSLEVSTL------GLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRP 59 Query: 62 RSEEMVGEAIRGIRDQVFLVSKVHPQNAGLDRITRSCEESLKRLGTDHLDLYLLHWRGKV 121 ++ + + + K+ + + + D ++ Sbjct: 60 ETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLK 119 Query: 122 PLEETVKGMEQLVASGKIARWGVSNLDTADMKELLRIPGGDRCAVNQVLYHLGSRGIEHE 181 L+ + Q+ + + A+ + R I Sbjct: 120 RLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVS 179 Query: 182 LLPWQRTHKIPVMAYSPLAQAGKLRKGVTENETVREIAEKHGATPLQIMLAWSIREGDII 241 + +A + + + + + Sbjct: 180 NETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFG 239 Query: 242 AIPKASSRKHVTENAEAAKIRLSEEECWKLDDAFPQPAWKVPLDMI 287 + A + + Sbjct: 240 TLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHG 285 >AGc3682 [R] COG0656 Aldo/keto reductases, related to diketogulonate reductase Length = 346 Score = 290 bits (742), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 15/279 (5%), Positives = 42/279 (15%), Gaps = 5/279 (1%) Query: 9 ITLKDGTAVTKMGQGTWNMGDSTATRDEEIAALRLGVELGMNLIDTAEMYGDGRSEEMVG 68 + + + + + A+ G+ L Sbjct: 8 HSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTET 67 Query: 69 EAIRGIRDQVFLVSKVHPQNAGLDRITRSCEESLKRLGTDHLDLYLLHWRGKVPLEETVK 128 + + + LH K + + Sbjct: 68 YVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLD 127 Query: 129 GMEQLVASGKIARWGVSNLDTADMKELLRIPGGDRCAVNQVLYHLGSRGIEHELLPWQRT 188 + +G D + + + Sbjct: 128 LYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVM 187 Query: 189 HKIPVMAYSPLAQAGKLRKGVTENETVREIAEKHGATPLQIMLAWSIREGDIIAIPKASS 248 + + L + + N + Sbjct: 188 RYLHLADKHDLPR-IVTIQ----NPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLT 242 Query: 249 RKHVTENAEAAKIRLSEEECWKLDDAFPQPAWKVPLDMI 287 K++ A + Q A +D+ Sbjct: 243 GKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIA 281 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.133 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,634,872 Number of extensions: 895871 Number of successful extensions: 21474 Number of sequences better than 1.0e-05: 6354 Number of HSP's gapped: 14865 Number of HSP's successfully gapped: 6356 Length of query: 287 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 182 Effective length of database: 26,268,361 Effective search space: 4780841702 Effective search space used: 4780841702 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 115 (48.9 bits)