BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0545.0031.p (487 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ZmtlA_1 [G] COG2213 Phosphotransferase system, mannitol-specific... 544 e-155 mtlA_1 [G] COG2213 Phosphotransferase system, mannitol-specific ... 544 e-155 ECs4475_1 [G] COG2213 Phosphotransferase system, mannitol-specif... 543 e-154 ZmtlA_1 [G] COG2213 Phosphotransferase system, mannitol-specific... 541 e-153 ZcmtA [G] COG2213 Phosphotransferase system, mannitol-specific I... 454 e-127 >ZmtlA_1 [G] COG2213 Phosphotransferase system, mannitol-specific IIBC component Length = 493 Score = 544 bits (1402), Expect = e-155, Method: Compositional matrix adjust. Identities = 282/475 (59%), Positives = 349/475 (73%), Gaps = 16/475 (3%) Query: 14 VRVKVQQFGRMLSGMVMPNIGAFIAWGLITALFIPTGWLPNKDLAMLVDPMIKFLLPLLI 73 +++KVQ FGR LS MVMPNIGAFIAWG+ITALFIPTGWLPN+ LA LV PMI +LLPLLI Sbjct: 5 IKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLPNETLAKLVGPMITYLLPLLI 64 Query: 74 GYTGGQMINGKRGGVIGAIATMGVIVGADIPMFLGAMIVGPLAGWVLRLFDRAVEGKIKA 133 GYTGG+++ G+RGGV+GAI TMGVIVGAD+PMFLG+MI GPL GW ++ FDR V+GKIK+ Sbjct: 65 GYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMIAGPLGGWCIKHFDRWVDGKIKS 124 Query: 134 GFEMLVNNFSXXXXXXXXXXXAYKGIGPLVQGLTNILSNGVEFLVNHNLLPLVNIIIEPA 193 GFEMLVNNFS A+ GIGP+V+ L+ +L+ GV F+V H++LPL +I +EPA Sbjct: 125 GFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKMLAAGVNFMVVHDMLPLASIFVEPA 184 Query: 194 KVLFLNNAINHGILSPIAAEESARIGKSILFMLESNPGPGLGILLAYWLVGKGSAKSSAP 253 K+LFLNNAINHGI SP+ ++S +GKSI F++E+NPGPG+G+LLAY G+GSAK SA Sbjct: 185 KILFLNNAINHGIFSPLGIQQSHELGKSIFFLIEANPGPGMGVLLAYMFFGRGSAKQSAG 244 Query: 254 GAVIIHFLGGIHEIYFPYILMNPRLILAVIGGGVSGTFTFQLLGAGLTSSPSPGSIFAYL 313 GA IIHFLGGIHEIYFPY+LMNPRLILAVI GG++G FT +LG GL S SPGSI A L Sbjct: 245 GAAIIHFLGGIHEIYFPYVLMNPRLILAVILGGMTGVFTLTILGGGLVSPASPGSILAVL 304 Query: 314 AMTPKGGYGPMFAGVIVATVVSFXXXXXXXKTSSKNNEDDVDLEEASAKMREMKXXXXXX 373 AMTPKG Y AGV A VSF KTS EDD+ E A+ +M++MK Sbjct: 305 AMTPKGAYFANIAGVCAAMAVSFVVSAILLKTSKVKEEDDI--EAATRRMQDMKAESKGA 362 Query: 374 XXXXXXXXXNVITPNVTANVRNK-ADVNKIVFACDAGMGSSAMGASVLRKKLQNAGVN-I 431 +P +V N + V KI+ ACDAGMGSSAMGA VLRKK+Q+AG++ I Sbjct: 363 ------------SPLSAGDVTNDLSHVRKIIVACDAGMGSSAMGAGVLRKKIQDAGLSQI 410 Query: 432 TVVNSAVSEIPADADIVVTQKTLTDRAIASNPNAEHISIDNFLKSPKYDELVERL 486 +V NSA++ +P D D+V+T + LT+RA+ P A+HIS+ NFL S Y L ERL Sbjct: 411 SVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLTNFLDSGLYTSLTERL 465 >mtlA_1 [G] COG2213 Phosphotransferase system, mannitol-specific IIBC component Length = 493 Score = 544 bits (1402), Expect = e-155, Method: Compositional matrix adjust. Identities = 282/475 (59%), Positives = 349/475 (73%), Gaps = 16/475 (3%) Query: 14 VRVKVQQFGRMLSGMVMPNIGAFIAWGLITALFIPTGWLPNKDLAMLVDPMIKFLLPLLI 73 +++KVQ FGR LS MVMPNIGAFIAWG+ITALFIPTGWLPN+ LA LV PMI +LLPLLI Sbjct: 5 IKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLPNETLAKLVGPMITYLLPLLI 64 Query: 74 GYTGGQMINGKRGGVIGAIATMGVIVGADIPMFLGAMIVGPLAGWVLRLFDRAVEGKIKA 133 GYTGG+++ G+RGGV+GAI TMGVIVGAD+PMFLG+MI GPL GW ++ FDR V+GKIK+ Sbjct: 65 GYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMIAGPLGGWCIKHFDRWVDGKIKS 124 Query: 134 GFEMLVNNFSXXXXXXXXXXXAYKGIGPLVQGLTNILSNGVEFLVNHNLLPLVNIIIEPA 193 GFEMLVNNFS A+ GIGP+V+ L+ +L+ GV F+V H++LPL +I +EPA Sbjct: 125 GFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKMLAAGVNFMVVHDMLPLASIFVEPA 184 Query: 194 KVLFLNNAINHGILSPIAAEESARIGKSILFMLESNPGPGLGILLAYWLVGKGSAKSSAP 253 K+LFLNNAINHGI SP+ ++S +GKSI F++E+NPGPG+G+LLAY G+GSAK SA Sbjct: 185 KILFLNNAINHGIFSPLGIQQSHELGKSIFFLIEANPGPGMGVLLAYMFFGRGSAKQSAG 244 Query: 254 GAVIIHFLGGIHEIYFPYILMNPRLILAVIGGGVSGTFTFQLLGAGLTSSPSPGSIFAYL 313 GA IIHFLGGIHEIYFPY+LMNPRLILAVI GG++G FT +LG GL S SPGSI A L Sbjct: 245 GAAIIHFLGGIHEIYFPYVLMNPRLILAVILGGMTGVFTLTILGGGLVSPASPGSILAVL 304 Query: 314 AMTPKGGYGPMFAGVIVATVVSFXXXXXXXKTSSKNNEDDVDLEEASAKMREMKXXXXXX 373 AMTPKG Y AGV A VSF KTS EDD+ E A+ +M++MK Sbjct: 305 AMTPKGAYFANIAGVCAAMAVSFVVSAILLKTSKVKEEDDI--EAATRRMQDMKAESKGA 362 Query: 374 XXXXXXXXXNVITPNVTANVRNK-ADVNKIVFACDAGMGSSAMGASVLRKKLQNAGVN-I 431 +P +V N + V KI+ ACDAGMGSSAMGA VLRKK+Q+AG++ I Sbjct: 363 ------------SPLSAGDVTNDLSHVRKIIVACDAGMGSSAMGAGVLRKKIQDAGLSQI 410 Query: 432 TVVNSAVSEIPADADIVVTQKTLTDRAIASNPNAEHISIDNFLKSPKYDELVERL 486 +V NSA++ +P D D+V+T + LT+RA+ P A+HIS+ NFL S Y L ERL Sbjct: 411 SVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLTNFLDSGLYTSLTERL 465 >ECs4475_1 [G] COG2213 Phosphotransferase system, mannitol-specific IIBC component Length = 492 Score = 543 bits (1400), Expect = e-154, Method: Compositional matrix adjust. Identities = 282/475 (59%), Positives = 349/475 (73%), Gaps = 16/475 (3%) Query: 14 VRVKVQQFGRMLSGMVMPNIGAFIAWGLITALFIPTGWLPNKDLAMLVDPMIKFLLPLLI 73 +++KVQ FGR LS MVMPNIGAFIAWG+ITALFIPTGWLPN+ LA LV PMI +LLPLLI Sbjct: 5 IKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLPNETLAKLVGPMITYLLPLLI 64 Query: 74 GYTGGQMINGKRGGVIGAIATMGVIVGADIPMFLGAMIVGPLAGWVLRLFDRAVEGKIKA 133 GYTGG+++ G+RGGV+GAI TMGVIVGAD+PMFLG+MI GPL GW ++ FDR V+GKIK+ Sbjct: 65 GYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMIAGPLGGWCIKHFDRWVDGKIKS 124 Query: 134 GFEMLVNNFSXXXXXXXXXXXAYKGIGPLVQGLTNILSNGVEFLVNHNLLPLVNIIIEPA 193 GFEMLVNNFS A+ GIGP+V+ L+ +L+ GV F+V H++LPL +I +EPA Sbjct: 125 GFEMLVNNFSAGIIGMILAILAFLGIGPIVETLSKMLAAGVNFMVVHDMLPLASIFVEPA 184 Query: 194 KVLFLNNAINHGILSPIAAEESARIGKSILFMLESNPGPGLGILLAYWLVGKGSAKSSAP 253 K+LFLNNAINHGI SP+ ++S +GKSI F++E+NPGPG+G+LLAY G+GSAK SA Sbjct: 185 KILFLNNAINHGIFSPLGIQQSHELGKSIFFLIEANPGPGMGVLLAYMFFGRGSAKQSAG 244 Query: 254 GAVIIHFLGGIHEIYFPYILMNPRLILAVIGGGVSGTFTFQLLGAGLTSSPSPGSIFAYL 313 GA IIHFLGGIHEIYFPY+LMNPRLILAVI GG++G FT +LG GL S SPGSI A L Sbjct: 245 GAAIIHFLGGIHEIYFPYVLMNPRLILAVILGGMTGVFTLTILGGGLVSPASPGSILAVL 304 Query: 314 AMTPKGGYGPMFAGVIVATVVSFXXXXXXXKTSSKNNEDDVDLEEASAKMREMKXXXXXX 373 AMTPKG Y AGV A VSF KTS EDD+ E A+ +M++MK Sbjct: 305 AMTPKGAYFANIAGVCAAMAVSFVVSAILLKTSKVKEEDDI--EAATRRMQDMKAESKGA 362 Query: 374 XXXXXXXXXNVITPNVTANVRNK-ADVNKIVFACDAGMGSSAMGASVLRKKLQNAGVN-I 431 +P +V N + V KI+ ACDAGMGSSAMGA VLRKK+Q+AG++ I Sbjct: 363 ------------SPLSAGDVTNDLSHVRKIIVACDAGMGSSAMGAGVLRKKIQDAGLSQI 410 Query: 432 TVVNSAVSEIPADADIVVTQKTLTDRAIASNPNAEHISIDNFLKSPKYDELVERL 486 +V NSA++ +P D D+V+T + LT+RA+ P A+HIS+ NFL S Y L ERL Sbjct: 411 SVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLTNFLDSGLYTSLTERL 465 Score = 541 bits (1393), Expect = e-153, Method: Compositional matrix adjust. Identities = 282/475 (59%), Positives = 349/475 (73%), Gaps = 16/475 (3%) Query: 14 VRVKVQQFGRMLSGMVMPNIGAFIAWGLITALFIPTGWLPNKDLAMLVDPMIKFLLPLLI 73 +++KVQ FGR LS MVMPNIGAFIAWG+ITALFIPTGWLPN+ LA LV PMI +LLPLLI Sbjct: 5 IKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLPNETLAKLVGPMITYLLPLLI 64 Query: 74 GYTGGQMINGKRGGVIGAIATMGVIVGADIPMFLGAMIVGPLAGWVLRLFDRAVEGKIKA 133 GYTGG+++ G+RGGV+GAI TMGVIVGAD+PMFLG+MI GPL GW ++ FDR V+GKIK+ Sbjct: 65 GYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMIAGPLGGWCIKHFDRWVDGKIKS 124 Query: 134 GFEMLVNNFSXXXXXXXXXXXAYKGIGPLVQGLTNILSNGVEFLVNHNLLPLVNIIIEPA 193 GFEMLVNNFS A+ GIGP+V+ L+ +L+ GV F+V H++LPL +I +EPA Sbjct: 125 GFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKMLAAGVNFMVVHDMLPLASIFVEPA 184 Query: 194 KVLFLNNAINHGILSPIAAEESARIGKSILFMLESNPGPGLGILLAYWLVGKGSAKSSAP 253 K+LFLNNAINHGI SP+ ++S +GKSI F++E+NPGPG+G+LLAY G+GSAK SA Sbjct: 185 KILFLNNAINHGIFSPLGIQQSHELGKSIFFLIEANPGPGMGVLLAYMFFGRGSAKQSAG 244 Query: 254 GAVIIHFLGGIHEIYFPYILMNPRLILAVIGGGVSGTFTFQLLGAGLTSSPSPGSIFAYL 313 GA IIHFLGGIHEIYFPY+LMNPRLILAVI GG++G FT +LG GL S SPGSI A L Sbjct: 245 GAAIIHFLGGIHEIYFPYVLMNPRLILAVILGGMTGVFTLTILGGGLVSPASPGSILAVL 304 Query: 314 AMTPKGGYGPMFAGVIVATVVSFXXXXXXXKTSSKNNEDDVDLEEASAKMREMKXXXXXX 373 AMTPKG Y AGV A VSF KTS EDD+ E A+ +M++MK Sbjct: 305 AMTPKGAYFANIAGVCAAMAVSFVVSAILLKTSKVKEEDDI--EAATRRMQDMKAESKGA 362 Query: 374 XXXXXXXXXNVITPNVTANVRNK-ADVNKIVFACDAGMGSSAMGASVLRKKLQNAGVN-I 431 +P +V N + V KI+ ACDAGMGSSAMGA VLRKK+Q+AG++ I Sbjct: 363 ------------SPLSAGDVTNDLSHVRKIIVACDAGMGSSAMGAGVLRKKIQDAGLSQI 410 Query: 432 TVVNSAVSEIPADADIVVTQKTLTDRAIASNPNAEHISIDNFLKSPKYDELVERL 486 +V NSA++ +P D D+V+T + LT+RA+ P A+HIS+ NFL S Y L ERL Sbjct: 411 SVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLTNFLDSGLYTSLTERL 465 >ZcmtA [G] COG2213 Phosphotransferase system, mannitol-specific IIBC component Length = 462 Score = 454 bits (1167), Expect = e-127, Method: Compositional matrix adjust. Identities = 234/473 (49%), Positives = 309/473 (65%), Gaps = 24/473 (5%) Query: 15 RVKVQQFGRMLSGMVMPNIGAFIAWGLITALFIPTGWLPNKDLAMLVDPMIKFLLPLLIG 74 R KVQ FG L+ MV+PNIGAFIAWG ITALFIPTGWLPN+ A +V PMI +LLP++IG Sbjct: 7 RAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWLPNEHFAKIVGPMITYLLPVMIG 66 Query: 75 YTGGQMINGKRGGVIGAIATMGVIVGADIPMFLGAMIVGPLAGWVLRLFDRAVEGKIKAG 134 TGG ++ GKRG V+G I T+GVIVGA+IPMFLG+MI+GPL G V++ D+A+E +I AG Sbjct: 67 STGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSMIMGPLGGLVIKYVDKALEKRIPAG 126 Query: 135 FEMLVNNFSXXXXXXXXXXXAYKGIGPLVQGLTNILSNGVEFLVNHNLLPLVNIIIEPAK 194 FEM++NNFS ++ IGP V + +E LV+ LPL+++I EPAK Sbjct: 127 FEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANTFVKECIEALVHAGYLPLLSVINEPAK 186 Query: 195 VLFLNNAINHGILSPIAAEESARIGKSILFMLESNPGPGLGILLAYWLVGKGSAKSSAPG 254 VLFLNNAI+ G+ P+ ++++ GKSI FM+ SNPGPGLG+LLA+ L GKG +K SAPG Sbjct: 187 VLFLNNAIDQGVYYPLGMQQASVNGKSIFFMVASNPGPGLGLLLAFTLFGKGMSKRSAPG 246 Query: 255 AVIIHFLGGIHEIYFPYILMNPRLILAVIGGGVSGTFTFQLLGAGLTSSPSPGSIFAYLA 314 A+IIHFLGGIHE+YFPY+LM P I+A+I GG+SGT+ F LL GL + PSPGSIFAYLA Sbjct: 247 AMIIHFLGGIHELYFPYVLMKPLTIIAMIAGGMSGTWMFNLLDGGLVAGPSPGSIFAYLA 306 Query: 315 MTPKGGYGPMFAGVIVATVVSFXXXXXXXKTSSK-NNEDDVDLEEASAKMREMKXXXXXX 373 +TPKG + AGV V T+VSF K E + + +++ ++ MK Sbjct: 307 LTPKGSFLATIAGVTVGTLVSFAITSLILKMEKTVETESEDEFAQSANAVKAMK------ 360 Query: 374 XXXXXXXXXNVITPNVTANVRNKADVNKIVFACDAGMGSSAMGASVLRKKLQNAGVNITV 433 + + V +I F CDAGMGSSAMGA+ RK+L+ AG+ I V Sbjct: 361 ----------------QEGAFSLSRVKRIAFVCDAGMGSSAMGATTFRKRLEKAGLAIEV 404 Query: 434 VNSAVSEIPADADIVVTQKTLTDRAIASNPNAEHISIDNFLKSPKYDELVERL 486 + A+ +PADAD VVT + R + + I I+N++ PK D L +L Sbjct: 405 KHYAIENVPADADXVVTHASXEGR-VKRVTDKPLILINNYIGDPKLDTLFNQL 456 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.139 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 33,012,503 Number of extensions: 1416380 Number of successful extensions: 3214 Number of sequences better than 1.0e-05: 10 Number of HSP's gapped: 3200 Number of HSP's successfully gapped: 11 Length of query: 487 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 377 Effective length of database: 25,691,691 Effective search space: 9685767507 Effective search space used: 9685767507 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 118 (50.1 bits)