BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0545.0027.p (242 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3217 [K] COG5662 Predicted transmembrane transcriptional regul... 49 9e-06 >BH3217 [K] COG5662 Predicted transmembrane transcriptional regulator (anti-sigma factor) Length = 240 Score = 48.5 bits (114), Expect = 9e-06, Method: Compositional matrix adjust. Identities = 33/141 (23%), Positives = 60/141 (42%), Gaps = 2/141 (1%) Query: 101 IMLLGLFGTEQDGRSERNRYVSSYEPQA-MAVLNRPETVAYPLD-WSRKDQFSGNVWYNG 158 +ML+ + G ++ +E Q + P T ++ S + +G VW N Sbjct: 97 LMLVVALASLNKGMDHDAVQIAPFEEQERLNFQQNPHTQQLDIEPLSYQPDLNGYVWVND 156 Query: 159 SSQELFVLIEDVALSDGLSIQAWAVKNGNRNTLGLIQIEDAKGHLYVKGSQLREADNIAL 218 + E+FV I+ ++ Q W + N +I I++ ++ G + I Sbjct: 157 VTHEIFVEIDGLSELQNQDHQLWIIFTDNNVQGAIIPIQEGSSRFFMHGMDVETLKLIKA 216 Query: 219 TVEPTGGSLSPTSPDAAWVHL 239 +VEP GGS +PT P+ V + Sbjct: 217 SVEPKGGSHTPTGPETFIVEI 237 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.134 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 13,441,726 Number of extensions: 442912 Number of successful extensions: 659 Number of sequences better than 1.0e-05: 1 Number of HSP's gapped: 659 Number of HSP's successfully gapped: 1 Length of query: 242 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 139 Effective length of database: 26,499,029 Effective search space: 3683365031 Effective search space used: 3683365031 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 114 (48.5 bits)