BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0545.0021.p (359 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3257 [G] COG1363 Cellulase M and related proteins 493 e-139 BS_ytoP [G] COG1363 Cellulase M and related proteins 478 e-135 lin1652 [G] COG1363 Cellulase M and related proteins 441 e-123 BH3132 [G] COG1363 Cellulase M and related proteins 351 1e-96 BS_ysdC [G] COG1363 Cellulase M and related proteins 340 2e-93 >BH3257 [G] COG1363 Cellulase M and related proteins Length = 357 Score = 493 bits (1268), Expect = e-139, Method: Compositional matrix adjust. Identities = 231/358 (64%), Positives = 286/358 (79%), Gaps = 2/358 (0%) Query: 1 MNPETLDLFKTLTEFPAAPGFERELRAYVKDALAPYTDEFVQDRLGSLXXXGVLRGEENG 60 MN ET LFKTLTE APGFE +R +V+ L YT+E VQDRLGS+ GV RG E G Sbjct: 1 MNQETQSLFKTLTELQGAPGFEHHIRRFVRGELEKYTNEIVQDRLGSIF--GVKRGNEQG 58 Query: 61 PKIMVAGHFDEVGFMVTGITDKGMIRFQPLGGWLASAVASQRLQIITPKGTLTGVVGSTP 120 PK+MVAGH DEVGFMVT I +KG+IRFQ LGGW + + +QR+QI+T +G + GV+GSTP Sbjct: 59 PKVMVAGHMDEVGFMVTSINEKGLIRFQTLGGWWSQVLLAQRVQIMTDEGPVIGVIGSTP 118 Query: 121 IHLQSDEERKQPGDIRKMYIDIGADSREEAQSFGVAPGQQILPVCPFTPLANPKKIMAKA 180 HL + +RK+P D++ MYIDIGAD +E+AQ G+ PGQQI+P+CPFTPLAN KKIMAKA Sbjct: 119 PHLLEEAQRKKPMDVKNMYIDIGADDKEDAQKIGIKPGQQIVPICPFTPLANEKKIMAKA 178 Query: 181 WDNRYGVGLAIELVQALHGKKLPNTVFAGATVQEEAGLRGARTAANLLQPDIYFALDASA 240 WDNRYGVGLAIEL++ L G+ PN +++GATVQEE GLRGA T+A +++PDI++ALDAS Sbjct: 179 WDNRYGVGLAIELLKELQGETTPNILYSGATVQEEVGLRGAATSAQMIEPDIFYALDASP 238 Query: 241 AADMTGDRQAFGQLGQGALLRIYDPTMITHRGLIEYVQDMADTHKIKYQYFVSQGGTDAG 300 A D T + AFGQLG+GAL+RIYD TM+THRG+ ++V D A+T I YQ+F+SQGGTDAG Sbjct: 239 ANDATAGKDAFGQLGKGALVRIYDRTMVTHRGIRDFVLDTAETENIPYQFFISQGGTDAG 298 Query: 301 QVHVSGIGVPSAVIGICSRYIHTASSIIHSDDYAAAKELLIKLVEGLDRTTLHTILEN 358 +VH+SG GVPSAVIGICSRYIHTA+SIIH DDYAAAK LL+KLV+ D+ + TIL N Sbjct: 299 RVHLSGNGVPSAVIGICSRYIHTAASIIHVDDYAAAKALLVKLVKTTDKAAVETILAN 356 >BS_ytoP [G] COG1363 Cellulase M and related proteins Length = 357 Score = 478 bits (1231), Expect = e-135, Method: Compositional matrix adjust. Identities = 225/358 (62%), Positives = 283/358 (79%), Gaps = 2/358 (0%) Query: 1 MNPETLDLFKTLTEFPAAPGFERELRAYVKDALAPYTDEFVQDRLGSLXXXGVLRGEENG 60 MN ET LF+TLT+ P APG E ++RA++K LA Y D+ VQDRLGS+ GV RG E+ Sbjct: 1 MNQETKALFQTLTQLPGAPGNEHQVRAFMKQELAKYADDIVQDRLGSVF--GVRRGAEDA 58 Query: 61 PKIMVAGHFDEVGFMVTGITDKGMIRFQPLGGWLASAVASQRLQIITPKGTLTGVVGSTP 120 P+IMVAGH DEVGFMVT ITD G++RFQ LGGW + + +QR++I T G + GV+ S P Sbjct: 59 PRIMVAGHMDEVGFMVTSITDNGLLRFQTLGGWWSQVLLAQRVEIQTDNGPVPGVISSIP 118 Query: 121 IHLQSDEERKQPGDIRKMYIDIGADSREEAQSFGVAPGQQILPVCPFTPLANPKKIMAKA 180 HL +D +R +P DI+ M IDIGAD +E+A G+ PGQQI+PVCPFT +AN KKI++KA Sbjct: 119 PHLLTDAQRNRPMDIKNMMIDIGADDKEDAIKIGIRPGQQIVPVCPFTTMANEKKILSKA 178 Query: 181 WDNRYGVGLAIELVQALHGKKLPNTVFAGATVQEEAGLRGARTAANLLQPDIYFALDASA 240 WDNRYG GL+IEL++ LHGK+LPNT++AGATVQEE GLRGA+TA+++++PD++FALDAS Sbjct: 179 WDNRYGCGLSIELLKELHGKELPNTLYAGATVQEEVGLRGAQTASHMIKPDLFFALDASP 238 Query: 241 AADMTGDRQAFGQLGQGALLRIYDPTMITHRGLIEYVQDMADTHKIKYQYFVSQGGTDAG 300 A DM+GD+ FGQLG+G LLRI D T + HRG+ E+V DMA+TH I YQYFVS GGTDAG Sbjct: 239 ANDMSGDKNEFGQLGKGFLLRILDRTTVMHRGMREFVLDMAETHDIPYQYFVSGGGTDAG 298 Query: 301 QVHVSGIGVPSAVIGICSRYIHTASSIIHSDDYAAAKELLIKLVEGLDRTTLHTILEN 358 +VH+S GVPSAVIGICSRYIHT ++IIH DDYAAAKE+LIKLV D+ T+ I EN Sbjct: 299 KVHISNSGVPSAVIGICSRYIHTNATIIHIDDYAAAKEMLIKLVTACDKQTVDAIKEN 356 >lin1652 [G] COG1363 Cellulase M and related proteins Length = 357 Score = 441 bits (1133), Expect = e-123, Method: Compositional matrix adjust. Identities = 208/359 (57%), Positives = 269/359 (74%), Gaps = 2/359 (0%) Query: 1 MNPETLDLFKTLTEFPAAPGFERELRAYVKDALAPYTDEFVQDRLGSLXXXGVLRGEENG 60 M ETL +FK LTE G E +RA+++ L P +DE +QD LG + GV G +G Sbjct: 1 MEKETLAMFKELTELQGTSGDEHRIRAFMRKELEPVSDEIIQDGLGGIF--GVRHGATDG 58 Query: 61 PKIMVAGHFDEVGFMVTGITDKGMIRFQPLGGWLASAVASQRLQIITPKGTLTGVVGSTP 120 P+++VA H DEVGFMVT ITD G+IRFQ +GGW + +QR+QI+ P G + GVV S P Sbjct: 59 PRVLVAAHMDEVGFMVTQITDNGLIRFQTIGGWNPQVLQAQRVQIMAPNGPVIGVVASVP 118 Query: 121 IHLQSDEERKQPGDIRKMYIDIGADSREEAQSFGVAPGQQILPVCPFTPLANPKKIMAKA 180 HL S+ ER +P D + + IDIGAD + +A+ G+ PGQ I+PV FTPLANPKKI+AKA Sbjct: 119 PHLLSEAERSKPTDPKNLLIDIGADDKADAEKIGIKPGQFIVPVAEFTPLANPKKILAKA 178 Query: 181 WDNRYGVGLAIELVQALHGKKLPNTVFAGATVQEEAGLRGARTAANLLQPDIYFALDASA 240 WDNRYGVGLAIEL++ L G+ LPNT+FAGA VQEE GLRGA +AN++QPD++FALDAS Sbjct: 179 WDNRYGVGLAIELMKELKGESLPNTLFAGANVQEEVGLRGAGVSANMIQPDLFFALDASP 238 Query: 241 AADMTGDRQAFGQLGQGALLRIYDPTMITHRGLIEYVQDMADTHKIKYQYFVSQGGTDAG 300 A D TG++ FGQ+GQG LLRI+D TMI HRG+ E++ D A+++KI YQYFVS GGTDAG Sbjct: 239 ANDTTGNKSQFGQIGQGFLLRIFDRTMIMHRGMREFLLDTAESNKIPYQYFVSPGGTDAG 298 Query: 301 QVHVSGIGVPSAVIGICSRYIHTASSIIHSDDYAAAKELLIKLVEGLDRTTLHTILENS 359 +VH S G+PSAVIG+ +RYIH+++SI+H DDYAAAKE++ L+ GLD+TT TI N+ Sbjct: 299 KVHTSLSGIPSAVIGVPARYIHSSNSILHVDDYAAAKEMITTLIRGLDKTTFETIKNNA 357 >BH3132 [G] COG1363 Cellulase M and related proteins Length = 361 Score = 351 bits (900), Expect = 1e-96, Method: Compositional matrix adjust. Identities = 166/355 (46%), Positives = 241/355 (67%), Gaps = 5/355 (1%) Query: 4 ETLDLFKTLTEFPAAPGFERELRAYVKDALAPYTDEFVQDRLGSLXXXGVLRGEENGPKI 63 E L + K LT+ PG ERE R ++ + P+ DE D+LGSL V G+E+GPKI Sbjct: 6 ERLQMLKELTDANGIPGNEREARDVMRKHIEPFADEVTTDKLGSLVAKKV--GKEDGPKI 63 Query: 64 MVAGHFDEVGFMVTGITDKGMIRFQPLGGWLASAVASQRLQIITPKGTLTGVVGSTPIHL 123 MVAGH DE+GFMVT I DKG +RFQ +GGW + +QR+ ++T KG + GV+GS P H+ Sbjct: 64 MVAGHLDEIGFMVTSIDDKGFLRFQTVGGWWEQVMLAQRVTVMTKKGNIPGVIGSKPPHI 123 Query: 124 QSDEERKQPGDIRKMYIDIGADSREEAQSFGVAPGQQILPVCPFTPLANPKKIMAKAWDN 183 E RK+P D + M+IDIG S+EEA FGV PG I+PVC FT + N K +MAKAWDN Sbjct: 124 LPAEARKKPVDKKDMFIDIGVASKEEALEFGVRPGDSIVPVCDFTVMKNEKYLMAKAWDN 183 Query: 184 RYGVGLAIELVQALHGKKLPNTVFAGATVQEEAGLRGARTAANLLQPDIYFALDASAAAD 243 R G +AI++++ L G PN V+ TVQEE GLRGART+A+ ++PDI F +D A D Sbjct: 184 RIGCAIAIDVLRELKGTNHPNVVYGVGTVQEEVGLRGARTSAHYIKPDIGFGVDVGIAGD 243 Query: 244 MTG--DRQAFGQLGQGALLRIYDPTMITHRGLIEYVQDMADTHKIKYQY-FVSQGGTDAG 300 G +++A ++G+G + +YD +M++H+GL ++V D+AD H I YQ+ ++ GGTD+G Sbjct: 244 TPGVTEKEALAKMGEGPQIVLYDASMVSHKGLRDFVTDIADEHNIPYQFDAMAGGGTDSG 303 Query: 301 QVHVSGIGVPSAVIGICSRYIHTASSIIHSDDYAAAKELLIKLVEGLDRTTLHTI 355 +H++ GVP+ I + +RYIH+ +SI+H DDY +L++++++ LDR T++ I Sbjct: 304 AIHLTANGVPALSITVATRYIHSHASILHRDDYENTVKLIVEVIKKLDRDTVNRI 358 >BS_ysdC [G] COG1363 Cellulase M and related proteins Length = 361 Score = 340 bits (873), Expect = 2e-93, Method: Compositional matrix adjust. Identities = 170/355 (47%), Positives = 238/355 (67%), Gaps = 5/355 (1%) Query: 4 ETLDLFKTLTEFPAAPGFERELRAYVKDALAPYTDEFVQDRLGSLXXXGVLRGEENGPKI 63 ETL + K LT+ PG ERE+R +K + P+ DE DRLGSL G ENGPKI Sbjct: 6 ETLTMLKDLTDAKGIPGNEREVRQVMKSYIEPFADEVTTDRLGSLIAKKT--GAENGPKI 63 Query: 64 MVAGHFDEVGFMVTGITDKGMIRFQPLGGWLASAVASQRLQIITPKGTLTGVVGSTPIHL 123 M+AGH DEVGFMVT ITDKG IRFQ +GGW A + +QR+ I+T KG +TGV+GS P H+ Sbjct: 64 MIAGHLDEVGFMVTQITDKGFIRFQTVGGWWAQVMLAQRVTIVTKKGEITGVIGSKPPHI 123 Query: 124 QSDEERKQPGDIRKMYIDIGADSREEAQSFGVAPGQQILPVCPFTPLANPKKIMAKAWDN 183 S E RK+ +I+ M+IDIGA SREEA +GV PG I+P FT + N K ++AKAWDN Sbjct: 124 LSPEARKKSVEIKDMFIDIGASSREEALEWGVLPGDMIVPHFEFTVMNNEKFLLAKAWDN 183 Query: 184 RYGVGLAIELVQALHGKKLPNTVFAGATVQEEAGLRGARTAANLLQPDIYFALDASAAAD 243 R G +AI++++ L PN V+ TVQEE GLRGA+TAA+ +QPDI F +D A D Sbjct: 184 RIGCAIAIDVLRNLQNTDHPNIVYGVGTVQEEVGLRGAKTAAHTIQPDIAFGVDVGIAGD 243 Query: 244 MTG--DRQAFGQLGQGALLRIYDPTMITHRGLIEYVQDMADTHKIKYQY-FVSQGGTDAG 300 G +++A ++G+G + +YD +M++H+GL + V A+ I YQ+ ++ GGTD+G Sbjct: 244 TPGISEKEAQSKMGKGPQIIVYDASMVSHKGLRDAVVATAEEAGIPYQFDAIAGGGTDSG 303 Query: 301 QVHVSGIGVPSAVIGICSRYIHTASSIIHSDDYAAAKELLIKLVEGLDRTTLHTI 355 +H++ GVP+ I I +RYIHT ++++H DDY A +L+ ++++ LDR T+ I Sbjct: 304 AIHLTANGVPALSITIATRYIHTHAAMLHRDDYENAVKLITEVIKKLDRKTVDEI 358 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.137 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 27,469,723 Number of extensions: 1109065 Number of successful extensions: 2314 Number of sequences better than 1.0e-05: 42 Number of HSP's gapped: 2201 Number of HSP's successfully gapped: 42 Length of query: 359 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 251 Effective length of database: 25,922,359 Effective search space: 6506512109 Effective search space used: 6506512109 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)