BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0545.0005.p
         (192 letters)

Database: COG 
           115,334 sequences; 38,378,431 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BH1327 [H] COG1713 Predicted HD superfamily hydrolase involved i...   164   7e-41
CAC1263 [H] COG1713 Predicted HD superfamily hydrolase involved ...   140   1e-33
L26998 [H] COG1713 Predicted HD superfamily hydrolase involved i...   122   2e-28
L132126 [H] COG1713 Predicted HD superfamily hydrolase involved ...    61   1e-09

>BH1327 [H] COG1713 Predicted HD superfamily hydrolase involved in NAD
           metabolism
          Length = 187

 Score =  164 bits (415), Expect = 7e-41,   Method: Compositional matrix adjust.
 Identities = 81/189 (42%), Positives = 119/189 (62%), Gaps = 2/189 (1%)

Query: 3   LSREALIEAVSAQMPDKRWKHTLGVMETSVKLAERYGGDPVRAETAAILHDVAKYWPVER 62
           ++R   ++ V   + + R++HT+GVMET++ LA+ YG D  +AE AAI HD AK+     
Sbjct: 1   MNRGKALQLVKPHLTEHRYQHTIGVMETAIDLAKLYGADQQKAELAAIFHDYAKFRDKNE 60

Query: 63  MKEIIEANGLSAELLSYDKQLWHAEVGAFVAGDEYGITDPEVLDAIRYHTSGRENMGLLE 122
           M+ +I       ++L Y  +L HA  GA+   +E GI D +VL AIR+HT+GR NM LLE
Sbjct: 61  MRTLIREKLSQQDILFYGDELLHAPCGAYYVREEVGIEDEDVLQAIRFHTTGRPNMSLLE 120

Query: 123 KIVCLADYIEPGRDFPGVDHIRKLSKASLEEGLLAGFDSTISLLLQKRQIVFPLTVLARN 182
           KI+ LADYIEP R FPGV+ +R  +K  L   +++   +TI+ LL+K Q ++P T+   N
Sbjct: 121 KIIFLADYIEPNRQFPGVEKVRTQAKTDLNGAIISSLVNTITFLLKKNQPIYPDTLATYN 180

Query: 183 DLVRILEDK 191
            L  +LE K
Sbjct: 181 QL--LLEQK 187


>CAC1263 [H] COG1713 Predicted HD superfamily hydrolase involved in NAD
           metabolism
          Length = 189

 Score =  140 bits (353), Expect = 1e-33,   Method: Compositional matrix adjust.
 Identities = 77/184 (41%), Positives = 112/184 (60%), Gaps = 3/184 (1%)

Query: 4   SREALIEAVSAQMPDKRWKHTLGVMETSVKLAERYGGDPVRAETAAILHDVAKYWPVERM 63
           S + + + +   + +KR+KH+LGVM+T+V+LA  Y  D  +A  A ++HD AK  P E++
Sbjct: 3   SYDKITDYLMNNLGEKRYKHSLGVMDTAVRLAGIYNEDTEKARIAGLVHDCAKKLPGEKI 62

Query: 64  KEIIEANG--LSAELLSYDKQLWHAEVGAFVAGDEYGITDPEVLDAIRYHTSGRENMGLL 121
            EI    G  L  E +  +  L H   G  +A    GI D +VL+AI +HT+GR NM LL
Sbjct: 63  IEICTNEGYELGDEDIR-NSYLLHGLAGRILAKKVIGIDDEDVLNAIEFHTTGRPNMSLL 121

Query: 122 EKIVCLADYIEPGRDFPGVDHIRKLSKASLEEGLLAGFDSTISLLLQKRQIVFPLTVLAR 181
           EKI+ +ADYIEPGR+F GVD +RK +   L + LL  FD+TI  ++ K   +   T+ AR
Sbjct: 122 EKIIYIADYIEPGREFKGVDELRKAADEDLNKALLMSFDNTIKFVIDKGGFLHHNTIEAR 181

Query: 182 NDLV 185
           N L+
Sbjct: 182 NYLI 185


>L26998 [H] COG1713 Predicted HD superfamily hydrolase involved in NAD
           metabolism
          Length = 311

 Score =  122 bits (307), Expect = 2e-28,   Method: Compositional matrix adjust.
 Identities = 13/186 (6%), Positives = 44/186 (23%)

Query: 3   LSREALIEAVSAQMPDKRWKHTLGVMETSVKLAERYGGDPVRAETAAILHDVAKYWPVER 62
           L     ++             T+ ++   + L+       +       +  VA       
Sbjct: 10  LESLKNLKRNGWMTVAAVSSVTITLVLVGLFLSVILNTSKISNGIENNVRIVAYMKLDVH 69

Query: 63  MKEIIEANGLSAELLSYDKQLWHAEVGAFVAGDEYGITDPEVLDAIRYHTSGRENMGLLE 122
            +E    +    +    +              +   +        ++  T    N   L 
Sbjct: 70  DQETKIQDPKDNKKTINNPDHLKIYDSIKKVPNVKTVEFSSKEAQLKELTKALGNNWNLF 129

Query: 123 KIVCLADYIEPGRDFPGVDHIRKLSKASLEEGLLAGFDSTISLLLQKRQIVFPLTVLARN 182
           K      Y     +     +++ ++    +   +       +   +  QI   +      
Sbjct: 130 KGDANPLYDAYIVEADKPANVKSVTSKISKLSGIEKATYGGANTAKLFQIAQGIRTWGTA 189

Query: 183 DLVRIL 188
             + ++
Sbjct: 190 AAILLI 195


>L132126 [H] COG1713 Predicted HD superfamily hydrolase involved in NAD
           metabolism
          Length = 197

 Score = 61.2 bits (147), Expect = 1e-09,   Method: Compositional matrix adjust.
 Identities = 45/133 (33%), Positives = 72/133 (54%), Gaps = 9/133 (6%)

Query: 6   EALIEAVSAQMP-DKRWKHTLGVMETSVKLAERYGGDPVR---AETAAILHDVAKYWPVE 61
           E LIE V+  +  D+ ++H L V +T+  L E       R   A  A ILHD+   +P +
Sbjct: 10  ELLIEEVNIFLNNDQLFEHCLAVAKTAEALTEELTDSNERRSQAFIAGILHDIGGIYPND 69

Query: 62  RMKEIIEANGLSAELLSYDKQ---LWHAEVGAFVAGDEYGITDPEVLDAIRYHTSGRENM 118
           +  E  +A   + ELL+ +++   + H ++  ++A   + ITD +VL AI  HT+ R+N 
Sbjct: 70  QRVE--KAEIFNIELLTEEREFPLIIHQKLSKYLASQHFKITDEQVLSAIECHTTLRKNF 127

Query: 119 GLLEKIVCLADYI 131
             L+ IV LAD I
Sbjct: 128 TELDLIVFLADKI 140


  Database: COG
    Posted date:  Aug 3, 2010  9:58 AM
  Number of letters in database: 38,378,431
  Number of sequences in database:  115,334
  
Lambda     K      H
   0.319    0.137    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 115334
Number of Hits to DB: 14,188,705
Number of extensions: 549274
Number of successful extensions: 1292
Number of sequences better than 1.0e-05: 4
Number of HSP's gapped: 1291
Number of HSP's successfully gapped: 4
Length of query: 192
Length of database: 38,378,431
Length adjustment: 101
Effective length of query: 91
Effective length of database: 26,729,697
Effective search space: 2432402427
Effective search space used: 2432402427
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 113 (48.1 bits)