BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0544.0046.p (238 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Rv0646c [R] COG0596 Predicted hydrolases or acyltransferases (al... 122 7e-28 MT0674 [R] COG0596 Predicted hydrolases or acyltransferases (alp... 122 7e-28 SMa1327 [R] COG0596 Predicted hydrolases or acyltransferases (al... 108 6e-24 RSc0298 [R] COG0596 Predicted hydrolases or acyltransferases (al... 70 4e-12 PA0480 [R] COG0596 Predicted hydrolases or acyltransferases (alp... 67 3e-11 >Rv0646c [R] COG0596 Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) Length = 301 Score = 122 bits (305), Expect = 7e-28, Method: Compositional matrix adjust. Identities = 85/214 (39%), Positives = 120/214 (56%), Gaps = 8/214 (3%) Query: 11 VELAYDSFGNENDEAIILISGLGTQMIRWTVPFCELLAARGFRVIRFDNRDVGCSTHFSQ 70 V+L Y+ G+ + ++LI GLG QM+ W FC L A+G RVIR+DNRDVG ST + Sbjct: 13 VKLYYEDMGDLDHPPVLLIMGLGAQMLLWRTDFCARLVAKGLRVIRYDNRDVGLSTKTER 72 Query: 71 YRTLDFDALATALMS---GQRPDIPYTLDDMAGDAIGLLDALSIDRAHLVGRSMGGMIAQ 127 +R LAT L+ G YTL+DMA DA LLD L + AH+VG SMGGMIAQ Sbjct: 73 HRP--GQPLATRLVRSWLGLPSQAAYTLEDMAADAAALLDHLDVKHAHVVGASMGGMIAQ 130 Query: 128 IAASEYPERVVSLTSIMSSSGNPTLPQASPE-VMGMMTKPAPNPFEDEAGFLGHSLSFAK 186 I A+ + +R +L I SS+ + LP +P ++ ++T P P+ D + +++ +K Sbjct: 131 IFAARFAQRTKTLAVIFSSNNHRFLPPPAPRALLALLTGPPPDSPRDV--IVDNAVRVSK 188 Query: 187 RIAGTGYPFEEDAYRALILEEVQRAYDPGSVGRQ 220 I YP ED RA E R + P + +Q Sbjct: 189 IIGSPAYPIPEDQVRAEAAESYDRNFHPWGIAQQ 222 >MT0674 [R] COG0596 Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) Length = 336 Score = 122 bits (305), Expect = 7e-28, Method: Compositional matrix adjust. Identities = 85/214 (39%), Positives = 120/214 (56%), Gaps = 8/214 (3%) Query: 11 VELAYDSFGNENDEAIILISGLGTQMIRWTVPFCELLAARGFRVIRFDNRDVGCSTHFSQ 70 V+L Y+ G+ + ++LI GLG QM+ W FC L A+G RVIR+DNRDVG ST + Sbjct: 48 VKLYYEDMGDLDHPPVLLIMGLGAQMLLWRTDFCARLVAKGLRVIRYDNRDVGLSTKTER 107 Query: 71 YRTLDFDALATALMS---GQRPDIPYTLDDMAGDAIGLLDALSIDRAHLVGRSMGGMIAQ 127 +R LAT L+ G YTL+DMA DA LLD L + AH+VG SMGGMIAQ Sbjct: 108 HRP--GQPLATRLVRSWLGLPSQAAYTLEDMAADAAALLDHLDVKHAHVVGASMGGMIAQ 165 Query: 128 IAASEYPERVVSLTSIMSSSGNPTLPQASPE-VMGMMTKPAPNPFEDEAGFLGHSLSFAK 186 I A+ + +R +L I SS+ + LP +P ++ ++T P P+ D + +++ +K Sbjct: 166 IFAARFAQRTKTLAVIFSSNNHRFLPPPAPRALLALLTGPPPDSPRDV--IVDNAVRVSK 223 Query: 187 RIAGTGYPFEEDAYRALILEEVQRAYDPGSVGRQ 220 I YP ED RA E R + P + +Q Sbjct: 224 IIGSPAYPIPEDQVRAEAAESYDRNFHPWGIAQQ 257 >SMa1327 [R] COG0596 Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) Length = 282 Score = 108 bits (270), Expect = 6e-24, Method: Compositional matrix adjust. Identities = 72/213 (33%), Positives = 104/213 (48%), Gaps = 25/213 (11%) Query: 3 MNILKVNGVELAYDSFGNENDEAIILISGLGTQMIRWTVPFCELLAARGFRVIRFDNRDV 62 M I+K N VE+A ++FG+ ++L+ G M+ W FC +A G VIR+D+RD Sbjct: 1 MLIIKGNDVEIATEAFGDSAHPPVVLVMGGMASMLWWPERFCRRVAEHGRFVIRYDHRDT 60 Query: 63 GCSTHFSQYRTLDFDALATALMSGQRPDIP-YTLDDMAGDAIGLLDALSIDRAHLVGRSM 121 G ST + P P Y D+ D + +LD I AH+VG S+ Sbjct: 61 GLSTKYP-------------------PGQPGYAFDNAVADVVRVLDGYRISAAHVVGMSL 101 Query: 122 GGMIAQIAASEYPERVVSLTSIMSSS---GNPTLPQASPEVMGMMTKPAPNPFEDEAGFL 178 GGMI Q A ++PERV+SLT+I SS LP + M M + D A + Sbjct: 102 GGMIGQATALKHPERVLSLTAISSSPVGMNTTHLPASGTAWMDHMNMEV--DWSDRAEAV 159 Query: 179 GHSLSFAKRIAGTGYPFEEDAYRALILEEVQRA 211 + L A+ +A T +PF+E RA I + R+ Sbjct: 160 AYMLEDARLVASTVHPFDEAETRAFIERDFDRS 192 >RSc0298 [R] COG0596 Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) Length = 279 Score = 69.7 bits (169), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 53/147 (36%), Positives = 68/147 (46%), Gaps = 24/147 (16%) Query: 9 NGVELAYDSFGNENDEAIILISGLGTQMIRWTVPFCELLAARGFRVIRFDNRDVGCSTHF 68 NG+ + Y G + I+L LG W LA+R RV+R+D R G +T Sbjct: 15 NGIRIRYRIDGADG-PWIMLAHALGVDHQMWD-SIAHRLASR-HRVLRYDARGHGGTT-- 69 Query: 69 SQYRTLDFDALATALMSGQRPDIPYTLDDMAGDAIGLLDALSIDRAHLVGRSMGGMIAQI 128 P YTL MA DA GLLDALSI + H VG SMGGM+AQI Sbjct: 70 -------------------APHGTYTLFQMADDAAGLLDALSIPQVHFVGLSMGGMVAQI 110 Query: 129 AASEYPERVVSLTSIMSSSGNPTLPQA 155 +P+R++SLT + P A Sbjct: 111 LGVRHPQRLLSLTLCDTVCHTPVAAHA 137 >PA0480 [R] COG0596 Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) Length = 265 Score = 66.6 bits (161), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 51/172 (29%), Positives = 75/172 (43%), Gaps = 25/172 (14%) Query: 9 NGVELAYDSFGNENDEAIILISGLGTQMIRWTVPFCELLAARGFRVIRFDNRDVGCSTHF 68 +G LAY G + L + +GT + W L R FRV+R+D R G S+ Sbjct: 11 DGASLAYRLDGAAEKPLLALSNSIGTTLHMWDAQLPAL--TRHFRVLRYDARGHGASS-- 66 Query: 69 SQYRTLDFDALATALMSGQRPDIPYTLDDMAGDAIGLLDALSIDRAHLVGRSMGGMIAQI 128 P PYTL + D + LLDAL + RAH +G S+GG++ Q Sbjct: 67 -------------------VPPGPYTLARLGEDVLELLDALEVRRAHFLGLSLGGIVGQW 107 Query: 129 AASEYPERVVSLTSIMSSSGNPTLPQASPEVMGMMTKPAPNPFEDEAGFLGH 180 A P+R+ L +S+ Q + ++ A + E AGFLG+ Sbjct: 108 LALHAPQRIERLVLANTSAWLGPAAQWDERIAAVLQ--AEDMSETAAGFLGN 157 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.137 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,629,471 Number of extensions: 695017 Number of successful extensions: 1445 Number of sequences better than 1.0e-05: 15 Number of HSP's gapped: 1448 Number of HSP's successfully gapped: 15 Length of query: 238 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 135 Effective length of database: 26,499,029 Effective search space: 3577368915 Effective search space used: 3577368915 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 114 (48.5 bits)