BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0544.0045.p (387 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA1626 [P] COG2807 Cyanate permease 123 4e-28 yeaN [P] COG2807 Cyanate permease 116 6e-26 ZyeaN [P] COG2807 Cyanate permease 116 6e-26 cynX [P] COG2807 Cyanate permease 115 1e-25 ZcynX [P] COG2807 Cyanate permease 115 1e-25 >PA1626 [P] COG2807 Cyanate permease Length = 424 Score = 123 bits (309), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 100/363 (27%), Positives = 171/363 (47%), Gaps = 14/363 (3%) Query: 18 RPMITSVASLLGTIQSHLGINALTASLLTTLPVLCMGIFSPVATVLSHRIGLERTIFFSL 77 RP ++S+A LL ++ G++A A LLTT PVLC+G+F+P+A L+ RIG ER++ L Sbjct: 54 RPALSSLAPLLNQVRDDTGLSAAAAGLLTTAPVLCLGLFAPLAPRLARRIGAERSVLLIL 113 Query: 78 FLITLATVLRGSDSSVMLLLVTALVGGIGISLAGPMLSSFIKKYFPEKPDIVSIYSISMT 137 F + VLR S V L +++ G I + G +L +K+ FP + +++ Sbjct: 114 FCLAGGIVLR-SLFPVFGLFAGSVLAGASIGIIGVLLPGIVKRDFPRQAGVMTGVYTMAL 172 Query: 138 IGAALASGFTIPVYTHSEYNLPIALSCWAILGFIALLAWIAFVRNKHHQTNATIRQRLPI 197 A + N +AL+ W++ +A W+ R H R Sbjct: 173 CLGAALAAGATAPLEKLFGNWELALAFWSLPAILAAAFWLPQTRQGQHAHRQAFRVIGLW 232 Query: 198 ANKKAIQFTIFFGLMASMFYSITSWISPIALSFGYSRESSAMFLTIFTLIQIPIAFLVPG 257 + A Q T++ GL +S+ Y + W+ I + G + + + L+ ++Q+ A L P Sbjct: 233 RDPLAWQVTLYMGLQSSLAYIVFGWLPSILIDRGMTAVEAGLVLSGSVMLQLITALLAPW 292 Query: 258 SVKRSGKPKXXXXXXXXXXXIGVGLLLFQFPMLLAV----LFLGIGAGGLFPLALMLPIS 313 R+ + GLL F + L + + LG+G GG F +AL L + Sbjct: 293 LAARARDQRLAVVLVMATTL--AGLLGFLYAPLQTIWGWAVLLGLGQGGTFSIALALIVL 350 Query: 314 ETKTAEEAGTWSAMSQMGGYVMGAFGPLLIGWISDQSGNFNSSIFAMLIIIMVMIGVQVS 373 ++ A A S M+Q GY + A GP ++G + D +G +N+ + + IGV ++ Sbjct: 351 RSRDAHVASHLSGMAQGVGYTLAAMGPFMVGVVHDLTGGWNA-------VGYIFIGVAIA 403 Query: 374 MTM 376 T+ Sbjct: 404 ATL 406 >yeaN [P] COG2807 Cyanate permease Length = 393 Score = 116 bits (291), Expect = 6e-26, Method: Compositional matrix adjust. Identities = 110/382 (28%), Positives = 174/382 (45%), Gaps = 22/382 (5%) Query: 14 AMNLRPMITSVASLLGTIQSHLGINALTASLLTTLPVLCMGIFSPVATVLSHRIGLERTI 73 A LR T A LL TI+S + LLTTLP+L + SP+A ++ R G+ER++ Sbjct: 23 ATTLRVTFTGAAPLLDTIRSAYSLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSL 82 Query: 74 FFSLFLITLATVLRGSDSSVMLLLVTALVGGIGISLAGPMLSSFIKKYFPEK-PDIVSIY 132 F +L LI +R S +L TA++GG GI+L +L IK+ FP + Y Sbjct: 83 FAALLLICAGIAIRSLPSPYLLFGGTAVIGG-GIALGNVLLPGLIKRDFPHSVARLTGAY 141 Query: 133 SISMTIGAALASGFTIPVYTHSEYNLPIALSCWAILGFIALLAWIAFVRNKHHQTNATIR 192 S++M AAL S +P+ + + AL +AL W+ R++ H +T R Sbjct: 142 SLTMGAAAALGSAMVVPLALNG-FGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSR 200 Query: 193 Q-------RLPIANKKAIQFTIFFGLMASMFYSITSWISPIALSFGYSRESSAMFLTIFT 245 R P+ A Q T+F G+ + ++Y I W+ I +S GYS + + Sbjct: 201 ALHTRGIWRSPL----AWQVTLFLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQ 256 Query: 246 LIQIPIAFLVPGSVKRSGKPKXXXXXXXXXXXIG-VGLLLFQFPMLLAVLFLGIGAGGLF 304 L L+P + + +G VGL + L G G+G Sbjct: 257 LATAAPGLLIPLFLHHVKDQRGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATM 316 Query: 305 PLALMLPISETKTAEEAGTWSAMSQMGGYVMGAFGPLLIGWISDQSGNFNSSIF--AMLI 362 L L +A +A S M+Q GY++ A GP L+G I D +GN++ + A+L Sbjct: 317 ILGLTFIGLRASSAHQAAALSGMAQSVGYLLAACGPPLMGKIHDANGNWSVPLMGVAILS 376 Query: 363 IIMVMIGVQVSMTMGKKDTEVQ 384 ++M + G+ +D E++ Sbjct: 377 LLMAIFGLCAG-----RDKEIR 393 >ZyeaN [P] COG2807 Cyanate permease Length = 393 Score = 116 bits (291), Expect = 6e-26, Method: Compositional matrix adjust. Identities = 110/382 (28%), Positives = 174/382 (45%), Gaps = 22/382 (5%) Query: 14 AMNLRPMITSVASLLGTIQSHLGINALTASLLTTLPVLCMGIFSPVATVLSHRIGLERTI 73 A LR T A LL TI+S + LLTTLP+L + SP+A ++ R G+ER++ Sbjct: 23 ATTLRVTFTGAAPLLDTIRSAYSLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSL 82 Query: 74 FFSLFLITLATVLRGSDSSVMLLLVTALVGGIGISLAGPMLSSFIKKYFPEK-PDIVSIY 132 F +L LI +R S +L TA++GG GI+L +L IK+ FP + Y Sbjct: 83 FAALLLICAGIAIRSLPSPYLLFGGTAVIGG-GIALGNVLLPGLIKRDFPHSVARLTGAY 141 Query: 133 SISMTIGAALASGFTIPVYTHSEYNLPIALSCWAILGFIALLAWIAFVRNKHHQTNATIR 192 S++M AAL S +P+ + + AL +AL W+ R++ H +T R Sbjct: 142 SLTMGAAAALGSAMVVPLALNG-FGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSR 200 Query: 193 Q-------RLPIANKKAIQFTIFFGLMASMFYSITSWISPIALSFGYSRESSAMFLTIFT 245 R P+ A Q T+F G+ + ++Y I W+ I +S GYS + + Sbjct: 201 ALHTRGIWRSPL----AWQVTLFLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQ 256 Query: 246 LIQIPIAFLVPGSVKRSGKPKXXXXXXXXXXXIG-VGLLLFQFPMLLAVLFLGIGAGGLF 304 L L+P + + +G VGL + L G G+G Sbjct: 257 LATAAPGLLIPLFLHHVKDQRGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATM 316 Query: 305 PLALMLPISETKTAEEAGTWSAMSQMGGYVMGAFGPLLIGWISDQSGNFNSSIF--AMLI 362 L L +A +A S M+Q GY++ A GP L+G I D +GN++ + A+L Sbjct: 317 ILGLTFIGLRASSAHQAAALSGMAQSVGYLLAACGPPLMGKIHDANGNWSVPLMGVAILS 376 Query: 363 IIMVMIGVQVSMTMGKKDTEVQ 384 ++M + G+ +D E++ Sbjct: 377 LLMAIFGLCAG-----RDKEIR 393 >cynX [P] COG2807 Cyanate permease Length = 384 Score = 115 bits (289), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 106/372 (28%), Positives = 177/372 (47%), Gaps = 17/372 (4%) Query: 7 LFALCIAAMNLRPMITSVASLLGTIQSHLGINALTASLLTTLPVLCMGIFSPVATVLSHR 66 L L + +N+RP++TSV LL ++ G++ A+LLT LPV+ MG + + L Sbjct: 2 LLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFSVAALLTALPVVTMGGLALAGSWLHQH 61 Query: 67 IGLERTIFFSLFLITLATVLRGSDSSVMLLLVTALVGGIGISLAGPMLSSFIKKYFPEK- 125 + R++ SL LI + ++R LLL +AL+GG+GI + ++ S IK+ F ++ Sbjct: 62 VSERRSVAISLLLIAVGALMRELYPQSALLLSSALLGGVGIGIIQAVMPSVIKRRFQQRT 121 Query: 126 PDIVSIYSISMTIGAALASGFTIPVYTHSE--YNLPIALSCWAILGFIALLAW----IAF 179 P ++ ++S ++ G L + T + HSE Y L+ WA+ +AL AW Sbjct: 122 PLVMGLWSAALMGGGGLGAAITPWLVQHSETWYQ---TLAWWALPAVVALFAWWWQSARE 178 Query: 180 VRNKHHQTNATIRQRLPIANKKAIQFTIFFGLMASMFYSITSWISPIALSFGYSRESSAM 239 V + H T +R + +A ++FGL+ + S+ +W+ + G S + S Sbjct: 179 VASSHKTTTTPVRV---VFTPRAWTLGVYFGLINGGYASLIAWLPAFYIEIGASAQYSGS 235 Query: 240 FLTIFTLIQIPIAFLVPGSVKRSGKPKXXXXXXXXXXX--IGVGLLLFQFPMLLAVLFLG 297 L + TL Q A L+P + + K G L Q P+L A++ G Sbjct: 236 LLALMTLGQAAGALLMPAMARHQDRRKLLMLALVLQLVGFCGFIWLPMQLPVLWAMV-CG 294 Query: 298 IGAGGLFPLALMLPISETKTAEEAGTWSAMSQMGGYVMGAFGPLLIGWISDQSGNF-NSS 356 +G GG FPL L+L + + AG A Q G+++ P G + SGN+ Sbjct: 295 LGLGGAFPLCLLLALDHSVQPAIAGKLVAFMQGIGFIIAGLAPWFSGVLRSISGNYLMDW 354 Query: 357 IFAMLIIIMVMI 368 F L ++ +MI Sbjct: 355 AFHALCVVGLMI 366 >ZcynX [P] COG2807 Cyanate permease Length = 384 Score = 115 bits (289), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 106/372 (28%), Positives = 177/372 (47%), Gaps = 17/372 (4%) Query: 7 LFALCIAAMNLRPMITSVASLLGTIQSHLGINALTASLLTTLPVLCMGIFSPVATVLSHR 66 L L + +N+RP++TSV LL ++ G++ A+LLT LPV+ MG + + L Sbjct: 2 LLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFSVAALLTALPVVTMGGLALAGSWLHQH 61 Query: 67 IGLERTIFFSLFLITLATVLRGSDSSVMLLLVTALVGGIGISLAGPMLSSFIKKYFPEK- 125 + R++ SL LI + ++R LLL +AL+GG+GI + ++ S IK+ F ++ Sbjct: 62 VSERRSVAISLLLIAVGALMRELYPQSALLLSSALLGGVGIGIIQAVMPSVIKRRFQQRT 121 Query: 126 PDIVSIYSISMTIGAALASGFTIPVYTHSE--YNLPIALSCWAILGFIALLAW----IAF 179 P ++ ++S ++ G L + T + HSE Y L+ WA+ +AL AW Sbjct: 122 PLVMGLWSAALMGGGGLGAALTPWLVQHSETWYQ---TLAWWALPAVVALFAWWWQSTRE 178 Query: 180 VRNKHHQTNATIRQRLPIANKKAIQFTIFFGLMASMFYSITSWISPIALSFGYSRESSAM 239 V + H T +R + +A ++FGL+ + S+ +W+ + G S + S Sbjct: 179 VASSHKTTTIPVRV---VFTPRAWTLGVYFGLINGGYASLIAWLPAFYIEIGASAQYSGS 235 Query: 240 FLTIFTLIQIPIAFLVPGSVKRSGKPKXXXXXXXXXXX--IGVGLLLFQFPMLLAVLFLG 297 L + TL Q A L+P + + K G L Q P+L A++ G Sbjct: 236 LLALMTLGQAAGALLMPAMARHQDRRKLLMLALALQLVGFCGFIWLPLQLPVLWAMV-CG 294 Query: 298 IGAGGLFPLALMLPISETKTAEEAGTWSAMSQMGGYVMGAFGPLLIGWISDQSGNF-NSS 356 +G GG FPL L+L + + AG A Q G+++ P G + SGN+ Sbjct: 295 LGLGGAFPLCLLLALDHSVQPAIAGKLVAFMQGIGFIIAGLAPWFSGVLRSISGNYLMDW 354 Query: 357 IFAMLIIIMVMI 368 F L ++ +MI Sbjct: 355 AFHALCVVGLMI 366 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.328 0.140 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,099,915 Number of extensions: 926140 Number of successful extensions: 5293 Number of sequences better than 1.0e-05: 17 Number of HSP's gapped: 5260 Number of HSP's successfully gapped: 17 Length of query: 387 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 279 Effective length of database: 25,922,359 Effective search space: 7232338161 Effective search space used: 7232338161 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 117 (49.7 bits)