BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0544.0039.p (254 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value VNG1340C [R] COG0491 Zn-dependent hydrolases, including glyoxylases 106 3e-23 SSO1537 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 92 6e-19 TVN1285 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 83 4e-16 DR0172 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 60 4e-09 RSp0595 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 54 2e-07 >VNG1340C [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 301 Score = 106 bits (265), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 83/241 (34%), Positives = 122/241 (50%), Gaps = 15/241 (6%) Query: 5 ATINNPNAQTQFVEFPIYTVLIDHPEGKILFDTACNPNSMGAQGRWAQSTQLSSPWVASE 64 AT+ +P+ + VE P+Y +LIDHP+G +L+DT +P++ G W + ++ Sbjct: 60 ATVADPDPEHALVECPVYNLLIDHPKGTVLWDTGSHPDA--GDGYWPTPLFEAFHHEDAD 117 Query: 65 ECYLHNRLEQLKIRPEEIKYVVASHLHLDHAGCLELF--TNATIIVHEDEFNGALQTYAR 122 E L L +I VVASHLHLDHAG L F T+ I VHE E A ++ A Sbjct: 118 EHTLPAALADAGTAISDIDAVVASHLHLDHAGGLRHFAGTDTPIYVHERELGYAYRSAAT 177 Query: 123 NEKEGAYVWADIDAWIKNQLQWKTIKPGEDNLDLVDGIKILNFGSGHAWGMLGLQIEMPE 182 + AY D D L W+ + L G+ +L+ GH G+LGL +E PE Sbjct: 178 DTGSIAYHSPDFD----RALNWRVVAVDRGRRQLYPGLDLLHL-PGHTPGLLGLYLE-PE 231 Query: 183 TGG--IILASDAIYTAESF--GPPVKPPGIIYDLIGYRNAVEKIRTLAARTNSQVWFGHD 238 G I+A D Y E++ G P+ ++ L +R + ++R LA RT + V+ GHD Sbjct: 232 DGAAPAIVAGDEAYQRENYEDGVPMA-TSLLSSLADWRESRRRVRDLARRTTADVFCGHD 290 Query: 239 T 239 T Sbjct: 291 T 291 >SSO1537 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 269 Score = 92.4 bits (228), Expect = 6e-19, Method: Compositional matrix adjust. Identities = 67/205 (32%), Positives = 103/205 (50%), Gaps = 18/205 (8%) Query: 3 NPATI---NNPNAQTQFVEFPIYTVLIDHPEGKILFDTACNPNSMGAQGRWAQSTQLSSP 59 NP TI NP + +V+ + + +I+H +G ILFDT N G+W Q P Sbjct: 24 NPMTIEDMKNPKPR-NWVDVSVMSAVIEHKDGVILFDTGIPDN---VSGKWPQIALSVFP 79 Query: 60 WVA-SEECYLHNRLEQLKIRPEEIKYVVASHLHLDHAGCLELFTNAT--IIVHEDEFNGA 116 SEE + N+L+++ +PE+I +++ SHLHLDH G LELF N+ +IVHE E A Sbjct: 80 VTKYSEENKMENQLKKIGYKPEDIHFIILSHLHLDHTGSLELFKNSKPDVIVHEKELKYA 139 Query: 117 LQTYARNEKEGAYVWADIDAWIKNQLQWKTIKP-GEDNLDLVDGIKILNFGSGHAWGMLG 175 L N K Y+ D++ L+ I P + L+++ G+++L G GH G + Sbjct: 140 LLNLWLN-KPVPYLLRDLEI-----LREMNIVPMSTEYLEILPGVELLLIG-GHTPGSIL 192 Query: 176 LQIEMPETGGIILASDAIYTAESFG 200 L++ I D I+ E Sbjct: 193 LKVRTQNDNNYIFTGDFIHVPEELS 217 >TVN1285 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 271 Score = 83.2 bits (204), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 58/193 (30%), Positives = 100/193 (51%), Gaps = 14/193 (7%) Query: 11 NAQTQFVEFPIYTVLIDHPEGKILFDTACNPNSMGAQGR-WAQSTQLSSPWVASEECYLH 69 N + +VE PI LIDHP+ +ILFDT P++M R ++ ++ ++ ++ Sbjct: 36 NPRRDWVEIPITGALIDHPDARILFDTGIAPDAMKTHERGLMEAFPITK---MDDQNHIE 92 Query: 70 NRLEQLKIRPEEIKYVVASHLHLDHAGCLELFTNA--TIIVHEDEFNGALQTYARNEKEG 127 N+L+ + ++PE+I +VV SHLHLDH G +F ++ IIV + E AL + K G Sbjct: 93 NQLKLIGLKPEDIDFVVISHLHLDHIGQAAVFKDSHVPIIVQKKELESALSLIWQG-KSG 151 Query: 128 AYVWADIDAWIKNQLQWKTIKPGEDNL-DLVDGIKILNFGSGHAWGMLGLQIEMPETGGI 186 AY ++D+ L+ P +D + ++VDG+ F GH G L ++ + Sbjct: 152 AYDYSDL-----LPLKGAAWMPLDDPVFEIVDGV-YAEFTGGHTAGHQVLTVKTEKGSMY 205 Query: 187 ILASDAIYTAESF 199 L D ++ + + Sbjct: 206 TLTGDFLHLPKEY 218 >DR0172 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 271 Score = 60.1 bits (144), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 54/231 (23%), Positives = 98/231 (42%), Gaps = 10/231 (4%) Query: 19 FPIYTVLIDHPEGKILFDTACNPNSMGAQGRWAQSTQLSSPWVASEECYLHNRLEQLKIR 78 P++ LI+HP G I+ DT + A+ W TQ + +E L ++++ Sbjct: 44 LPVWCWLIEHPSGLIVVDTGL---TCTARSNWFSRTQTRLHY--TEHDRLSRQMQRAGFD 98 Query: 79 PEEIKYVVASHLHLDHAGCLELFTNATIIVHEDEFNGALQTYARNEKEGAYVWADIDAWI 138 P +++ ++ +HLH+DH + F A ++V E AR A W + Sbjct: 99 PAQVRVLILTHLHIDHDRGMGDFPEAHLLVSARELRDYRSPLARLMGYSAPTWKSVPH-- 156 Query: 139 KNQLQWKTIKPGEDNLDLV-DGIKILNFGSGHAWGMLGLQIEMPETGGIILASDAIYTAE 197 P ++ D++ DG +L GH G L + + P I+LA D Y+ + Sbjct: 157 AAPFLPLAHGPFRESADVMGDGRVLLLPTPGHTPGHLSVLVRQP-GHDILLAGDLTYSEQ 215 Query: 198 SFGPPVKPPGIIYDLIGYRNAVEKIRTLAARTNSQVWFGHDTDQFKTLRKS 248 + GI+ +R ++ ++R LA HD D + L+++ Sbjct: 216 DLR-TGRLGGIVPSPHIHRKSMRQVRQLAQLRPLIYLPSHDPDSLRRLQRN 265 >RSp0595 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 136 Score = 53.9 bits (128), Expect = 2e-07, Method: Compositional matrix adjust. Identities = 31/97 (31%), Positives = 51/97 (52%), Gaps = 2/97 (2%) Query: 158 DGIKILNFGSGHAWGMLGLQIEMPETGGIILASDAIYTAESFGPPVKPPGIIYDLIGYRN 217 DG +L GH G L +++P+TG ++L+ DA++ ++ + P I +D R Sbjct: 42 DGSVMLISTPGHTPGHQSLLVKLPKTGALVLSGDAVHFQSNWD-NRRVPSINFDQDKTRA 100 Query: 218 AVEKIRTLAARTNSQVWFGHDTDQFKTLRKSTEGYYE 254 ++++I L R +Q+W HD Q TL K YYE Sbjct: 101 SMQRIADLLVREKAQLWINHDKAQRDTL-KMAPAYYE 136 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.135 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,725,032 Number of extensions: 840193 Number of successful extensions: 3219 Number of sequences better than 1.0e-05: 7 Number of HSP's gapped: 3217 Number of HSP's successfully gapped: 7 Length of query: 254 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 150 Effective length of database: 26,383,695 Effective search space: 3957554250 Effective search space used: 3957554250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 114 (48.5 bits)