BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0544.0033.p (223 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC0884 [T] COG0664 cAMP-binding proteins - catabolite gene acti... 89 4e-18 FN1901 [T] COG0664 cAMP-binding proteins - catabolite gene activ... 64 2e-10 >CAC0884 [T] COG0664 cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases Length = 229 Score = 89.4 bits (220), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 56/207 (27%), Positives = 96/207 (46%), Gaps = 4/207 (1%) Query: 12 LFSGISPNDIPQMLKCLSATRKEYAKDEMVVREGDLVDDVGIILQGSAQSTKLSVKGKQI 71 LFS I +I + ++ + KEY KDE + EGD + +GI+L G + K+ GK + Sbjct: 14 LFSKIETQEIDHLFSSINYSIKEYYKDETIAIEGDTCNKIGIVLSGCVEIQKIYESGKSL 73 Query: 72 IVSLHYPGGYTAVLTAASRGRRCPMSVQAIEPLEILFIPIKSILSRCTKLCMAHEQLLGN 131 ++ S P ++ A I+FIP SI +KLC + L Sbjct: 74 TITTLEESKIFGEAIIFSNKTSYPSTIIACTKSTIIFIPKASI----SKLCSDNSLFLNG 129 Query: 132 LFDSIAERALELHDRNDCLIMPTIRDKVLTYLTRVMRETGTETFTIPFDREAMAEYLDVD 191 ++ + L L+ + + TIR+K+ Y+ TF + ++ ++E L + Sbjct: 130 FMSLLSNKILMLNKKLKNMSYHTIREKISNYILEQYEAQNNLTFKMNKSKKQLSEMLGIP 189 Query: 192 RSALSRELAWMKRDGLIDFYRNEFKLL 218 R +LSRE ++ +G+IDF R +L Sbjct: 190 RPSLSREFINLREEGIIDFDRTSITIL 216 >FN1901 [T] COG0664 cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases Length = 217 Score = 63.9 bits (154), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 55/217 (25%), Positives = 104/217 (47%), Gaps = 10/217 (4%) Query: 4 LDSLCQSRLFSGISPNDIPQMLKCLSATRKEYAKDEMVVREGDLVDDVGIILQGSAQSTK 63 + +L ++ +F+ I I +L+ K+Y+ +E + GD V + IIL+G+ + Sbjct: 2 IKTLKETVVFNSIDEKTIKNILEKTKYEIKKYSPNESIAFRGDEVKGLYIILKGTLITEM 61 Query: 64 LSVKGKQIIVSLHYPGGYTAVLTAASRGRRCPMSVQAIEPLEILFIPIKSILSRCTKLCM 123 L+ +G I + P A + P+ + + EILF+ K L KL Sbjct: 62 LTEEGNVIKIEELVPSDVIASAFIFGKKNSFPVDLSVKDEAEILFVERKEFL----KLLF 117 Query: 124 AHEQLLGNLFDSIAERALELHDR-NDCLIMPTIRDKVLTYLTRVMRETGTETFTIPFDRE 182 + E++L N + I+ + L ++ + TI+ K Y V + F+I + Sbjct: 118 SEEKILENFLNEISNKTQLLTNKIWNSFNNKTIKKKFCDY---VKKNQKNNLFSIQ-NLG 173 Query: 183 AMAEYLDVDRSALSRELAWMKRDGLID-FYRNEFKLL 218 A+AE+ V+R +LSR L+ + +D ++ RN++K+L Sbjct: 174 ALAEFFGVERPSLSRVLSELVKDEKLERIGRNKYKIL 210 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.137 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 14,937,037 Number of extensions: 522310 Number of successful extensions: 1688 Number of sequences better than 1.0e-05: 3 Number of HSP's gapped: 1694 Number of HSP's successfully gapped: 3 Length of query: 223 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 121 Effective length of database: 26,614,363 Effective search space: 3220337923 Effective search space used: 3220337923 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 114 (48.5 bits)