BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0544.0027.p (284 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MT0455 [IQR] COG1028 Dehydrogenases with different specificities... 117 1e-26 Rv0439c [IQR] COG1028 Dehydrogenases with different specificitie... 117 2e-26 MT0074 [IQR] COG1028 Dehydrogenases with different specificities... 114 2e-25 VNG0479G [IQR] COG1028 Dehydrogenases with different specificiti... 113 3e-25 Rv0068 [IQR] COG1028 Dehydrogenases with different specificities... 112 5e-25 >MT0455 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 338 Score = 117 bits (294), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 86/289 (29%), Positives = 139/289 (48%), Gaps = 14/289 (4%) Query: 8 DIENRVFLISGGTSGVGKAIATGLARLGAKVVIISRSAESGQAALKDISEATGNNRGDWV 67 D RV +++G +G+G A A GA VV+ R+ E G AA I A Sbjct: 45 DQSGRVVVVTGANTGIGYHTAAVFADRGAHVVLAVRNLEKGNAARARIMAARPGAHVTLQ 104 Query: 68 VADLSLQSSIREVSDVIKRKYDQLHVLVNACGAIYFKKELTAEGIDRTFAVNVLSHYLLT 127 DL S+R +D ++ Y ++ VL+N G ++ K++T +G + F N L H+ LT Sbjct: 105 QLDLCSLDSVRAAADALRTAYPRIDVLINNAGVMWTPKQVTKDGFELQFGTNHLGHFALT 164 Query: 128 NRMLDILQESGPARVITVSGNPRFFKNPVINFDDIQMERQYSGMSAANQSLFAKTLFAFE 187 +LD + +RV+TVS + I+FDD+Q ER+Y+ ++A Q+ A LF +E Sbjct: 165 GLVLDHMLPVPGSRVVTVSSQGHRI-HAAIHFDDLQWERRYNRVAAYGQAKLANLLFTYE 223 Query: 188 LARRL--EGTGVTSNAFYPGFVKSNLSRNAPWYLRAAAPLMNAWVKAECEIGVY--LASA 243 L RRL G + A +PG + L+RN P +R A ++ + E+G L +A Sbjct: 224 LQRRLGEAGKSTIAVAAHPGGSNTELTRNLPRLIRPVATVLGPLLFQSPEMGALPTLRAA 283 Query: 244 KELERTNGVFFDD----KKQTVPIYTK-----FDDGAGNKLWTLCEGLT 283 + G ++ +++ P + D +LWT+ E LT Sbjct: 284 TDPTTQGGQYYGPDGFGEQRGHPKVVQSSAQSHDKDLQRRLWTVSEELT 332 >Rv0439c [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 311 Score = 117 bits (294), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 86/289 (29%), Positives = 139/289 (48%), Gaps = 14/289 (4%) Query: 8 DIENRVFLISGGTSGVGKAIATGLARLGAKVVIISRSAESGQAALKDISEATGNNRGDWV 67 D RV +++G +G+G A A GA VV+ R+ E G AA I A Sbjct: 18 DQSGRVVVVTGANTGIGYHTAAVFADRGAHVVLAVRNLEKGNAARARIMAARPGAHVTLQ 77 Query: 68 VADLSLQSSIREVSDVIKRKYDQLHVLVNACGAIYFKKELTAEGIDRTFAVNVLSHYLLT 127 DL S+R +D ++ Y ++ VL+N G ++ K++T +G + F N L H+ LT Sbjct: 78 QLDLCSLDSVRAAADALRTAYPRIDVLINNAGVMWTPKQVTKDGFELQFGTNHLGHFALT 137 Query: 128 NRMLDILQESGPARVITVSGNPRFFKNPVINFDDIQMERQYSGMSAANQSLFAKTLFAFE 187 +LD + +RV+TVS + I+FDD+Q ER+Y+ ++A Q+ A LF +E Sbjct: 138 GLVLDHMLPVPGSRVVTVSSQGHRI-HAAIHFDDLQWERRYNRVAAYGQAKLANLLFTYE 196 Query: 188 LARRL--EGTGVTSNAFYPGFVKSNLSRNAPWYLRAAAPLMNAWVKAECEIGVY--LASA 243 L RRL G + A +PG + L+RN P +R A ++ + E+G L +A Sbjct: 197 LQRRLGEAGKSTIAVAAHPGGSNTELTRNLPRLIRPVATVLGPLLFQSPEMGALPTLRAA 256 Query: 244 KELERTNGVFFDD----KKQTVPIYTK-----FDDGAGNKLWTLCEGLT 283 + G ++ +++ P + D +LWT+ E LT Sbjct: 257 TDPTTQGGQYYGPDGFGEQRGHPKVVQSSAQSHDKDLQRRLWTVSEELT 305 >MT0074 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 303 Score = 114 bits (285), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 90/288 (31%), Positives = 144/288 (50%), Gaps = 14/288 (4%) Query: 8 DIENRVFLISGGTSGVGKAIATGLARLGAKVVIISRSAESGQAALKDISEATGNNRGDWV 67 D R +I+G +G+G A LA GA VV+ R+ + G+ A I+EAT + Sbjct: 11 DQTGRTAVITGANTGLGFETAAALAAHGAHVVLAVRNLDKGKQAAARITEATPGAEVELQ 70 Query: 68 VADLSLQSSIREVSDVIKRKYDQLHVLVNACGAIYFKKELTAEGIDRTFAVNVLSHYLLT 127 DL+ +S+R + +K + ++ +L+N G +Y ++ TA+G + F N L H+ LT Sbjct: 71 ELDLTSLASVRAAAAQLKSDHQRIDLLINNAGVMYTPRQTTADGFEMQFGTNHLGHFALT 130 Query: 128 NRMLDILQESGPARVITVSGNPRFFKNPVINFDDIQMERQYSGMSAANQSLFAKTLFAFE 187 ++D L +RV+T+S + I+FDD+Q ER+Y ++A Q+ A LF +E Sbjct: 131 GLLIDRLLPVAGSRVVTISSVGHRIRA-AIHFDDLQWERRYRRVAAYGQAKLANLLFTYE 189 Query: 188 LARRLEGTGVT-SNAFYPGFVKSNLSRNAPWYLRAAAPLMNAWVKAECEIGVY--LASAK 244 L RRL G T + A +PG + L RN P L A A ++ A + + E+G L +A Sbjct: 190 LQRRLAPGGTTIAVASHPGVSNTELVRNMPRPLVAVAAIL-APLMQDAELGALPTLRAAT 248 Query: 245 ELERTNGVFF-DDKKQTVPIYTK--------FDDGAGNKLWTLCEGLT 283 + G +F D + Y K D+ +LW + E LT Sbjct: 249 DPAVRGGQYFGPDGFGEIRGYPKVVASSAQSHDEQLQRRLWAVSEELT 296 >VNG0479G [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 316 Score = 113 bits (283), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 78/215 (36%), Positives = 110/215 (51%), Gaps = 11/215 (5%) Query: 8 DIENRVFLISGGTSGVGKAIATGLARLGAKVVIISRSAESGQAALKDI-SEATGNNRGDW 66 D R +++G SG+G AR GA VV+ RS E G+ A DI +E G + Sbjct: 14 DQSGRRVVVTGANSGLGFEATRAFARAGAHVVMACRSTERGEDARDDIVAELPGAS---L 70 Query: 67 VVADLSLQS--SIREVSDVIKRKYDQLHVLVNACGAIYFKKELTAEGIDRTFAVNVLSHY 124 V +L L + S+ +D ++D LHVL N G + + TA+G + F VN L H Sbjct: 71 TVHELDLAALDSVAAFADWFTAEFDSLHVLANNAGVMAIPRSETADGFETQFGVNHLGHV 130 Query: 125 LLTNRMLDILQE-SGPARVITVSGNPRFFKNPVINFDDIQMERQYSGMSAANQSLFAKTL 183 LT +L +L+ SG RV+T S + I+F+D+Q E +Y A +QS A L Sbjct: 131 ALTAGLLGVLRRTSGETRVVTQSSGA--HRRGRIDFEDLQHEAEYGKWEAYSQSKLANLL 188 Query: 184 FAFELARRLEG--TGVTSNAFYPGFVKSNLSRNAP 216 FA+EL RRL VTS A +PG+ +NL P Sbjct: 189 FAYELDRRLRAASASVTSVACHPGYAATNLQLRGP 223 >Rv0068 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 303 Score = 112 bits (281), Expect = 5e-25, Method: Compositional matrix adjust. Identities = 89/288 (30%), Positives = 144/288 (50%), Gaps = 14/288 (4%) Query: 8 DIENRVFLISGGTSGVGKAIATGLARLGAKVVIISRSAESGQAALKDISEATGNNRGDWV 67 D R +I+G +G+G A LA GA VV+ R+ + G+ A I+EAT + Sbjct: 11 DQTGRTAVITGANTGLGFETAAALAAHGAHVVLAVRNLDKGKQAAARITEATPGAEVELQ 70 Query: 68 VADLSLQSSIREVSDVIKRKYDQLHVLVNACGAIYFKKELTAEGIDRTFAVNVLSHYLLT 127 DL+ +S+R + +K + ++ +L+N G +Y ++ TA+G + F N L H+ LT Sbjct: 71 ELDLTSLASVRAAAAQLKSDHQRIDLLINNAGVMYTPRQTTADGFEMQFGTNHLGHFALT 130 Query: 128 NRMLDILQESGPARVITVSGNPRFFKNPVINFDDIQMERQYSGMSAANQSLFAKTLFAFE 187 ++D L +RV+T+S + I+FDD+Q ER+Y ++A Q+ A LF +E Sbjct: 131 GLLIDRLLPVAGSRVVTISSVGHRIRA-AIHFDDLQWERRYRRVAAYGQAKLANLLFTYE 189 Query: 188 LARRLEGTGVT-SNAFYPGFVKSNLSRNAPWYLRAAAPLMNAWVKAECEIGVY--LASAK 244 L RRL G T + A +PG + + RN P L A A ++ A + + E+G L +A Sbjct: 190 LQRRLAPGGTTIAVASHPGVSNTEVVRNMPRPLVAVAAIL-APLMQDAELGALPTLRAAT 248 Query: 245 ELERTNGVFF-DDKKQTVPIYTK--------FDDGAGNKLWTLCEGLT 283 + G +F D + Y K D+ +LW + E LT Sbjct: 249 DPAVRGGQYFGPDGFGEIRGYPKVVASSAQSHDEQLQRRLWAVSEELT 296 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.133 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,022,338 Number of extensions: 788541 Number of successful extensions: 2703 Number of sequences better than 1.0e-05: 121 Number of HSP's gapped: 2702 Number of HSP's successfully gapped: 121 Length of query: 284 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 179 Effective length of database: 26,268,361 Effective search space: 4702036619 Effective search space used: 4702036619 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)