BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0544.0024.p (377 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2515 [T] COG0642 Signal transduction histidine kinase 158 1e-38 BH1154_2 [T] COG0642 Signal transduction histidine kinase 133 4e-31 TM1654_2 [T] COG0642 Signal transduction histidine kinase 119 7e-27 Cgl2555 [T] COG0642 Signal transduction histidine kinase 115 8e-26 MT3343 [T] COG0642 Signal transduction histidine kinase 115 1e-25 >lin2515 [T] COG0642 Signal transduction histidine kinase Length = 182 Score = 158 bits (400), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 0/303 (0%), Positives = 0/303 (0%), Gaps = 11/303 (3%) Query: 66 FLLVFTIT--FFGLTRRIVKDLMTLERGLQMITEGDLNHRVPVHRQD---ELGRVAYNIN 120 + ++ + + Sbjct: 83 RCRYELGVEITPPESIYPDFRYR-ATDPSGIVENE-VCPVFAARTTSALQINDDEVMDYQ 140 Query: 121 QMTERLQLQIAKERENERSKMELITGISHDLRTPLTSIIGYIELLRTKSFQDQEEYTRFV 180 Sbjct: 141 200 Query: 181 QNTYNKSIHLKKLLDDLFEYTRLNSIDTRQNVHRIDXXXXXXXXXXXXXPIAHDNAIHII 240 Sbjct: 201 260 Query: 241 KDIGSSPVLIFVDSDKIARAIDNLLMNALKYSVKPGAIHVRMITDPKQAAIEVENRGIPL 300 Sbjct: 261 320 Query: 301 TREQQEKLFERFYKVDHSRSSEGIQTGAGLGLSIARNIAERHGGTLTLDHVNDVFTFRLT 360 Sbjct: 321 ---- 376 Query: 361 IPI 363 Sbjct: 377 379 >BH1154_2 [T] COG0642 Signal transduction histidine kinase Length = 274 Score = 133 bits (335), Expect = 4e-31, Method: Compositional matrix adjust. Identities = 85/269 (31%), Positives = 142/269 (52%), Gaps = 7/269 (2%) Query: 95 ITEGDLNHRVPVHRQDELGRVAYNINQMTERLQLQIAKERENERSKMELITGISHDLRTP 154 I G +PV + L +A NIN M + ++ ++ ++ER K ELIT +SHDLRTP Sbjct: 11 IKSGHTPQDLPVRGRSALALLAQNINTMKKGMKQSEREQAKSERLKTELITNVSHDLRTP 70 Query: 155 LTSIIGYIELLRTKSFQDQEEYTRFVQNTYNKSIHLKKLLDDLFEYTRLNSIDTRQNVHR 214 LTSII Y ELL+ Q++ +Y + K+ LK L+DDLFE ++++S + + Sbjct: 71 LTSIITYTELLKDADEQERADYIEIID---RKARRLKILIDDLFEASKMSSGNIELTLET 127 Query: 215 IDXXXXXXXXXXXXXPIAHDNAIHIIKDIGSSPVLIFVDSDKIARAIDNLLMNALKYSVK 274 +D +++ + + P+ I D KI R DNL+ N LKYS++ Sbjct: 128 VDVLQLLQQALAEYGEALEQSSLQLRISVPEEPLYIKADGQKIWRVFDNLIQNILKYSLE 187 Query: 275 PGAIHVRMITDPKQAAIEVENRGIPLTREQQEKLFERFYKVDHSRSSEGIQTGAGLGLSI 334 +++ + + I ++N + ++L ERF + D SR++E G+GLGL+I Sbjct: 188 HTRVYLSVKEKKETVEIIIKNVAKYELGDNVDELLERFKRGDQSRNTE----GSGLGLAI 243 Query: 335 ARNIAERHGGTLTLDHVNDVFTFRLTIPI 363 A++I + H G L +D D+F + +P+ Sbjct: 244 AKSIVDLHEGYLDIDVDGDLFKVIIQLPM 272 >TM1654_2 [T] COG0642 Signal transduction histidine kinase Length = 272 Score = 119 bits (299), Expect = 7e-27, Method: Compositional matrix adjust. Identities = 71/238 (29%), Positives = 129/238 (54%), Gaps = 9/238 (3%) Query: 130 IAKERENERSKMELITGISHDLRTPLTSIIGYIELLRTKSFQDQEEYTRFVQNTYNKSIH 189 + KER+ + + E I +SH+LRTPLTSI GY E L +++E RF++ +S Sbjct: 36 VTKERKLDEMRREFIATVSHELRTPLTSIHGYAETLLEDDLENKELVKRFLKIIEEESAR 95 Query: 190 LKKLLDDLFEYTRLNSIDTRQNVHRIDXXXXXXXXXXXXXPIAHDNAIHIIKDIGSSPVL 249 + +L++DL + ++ + + +D PIA +N + +I + V+ Sbjct: 96 MTRLINDLLDLEKIEESEANFEMKDVDLCEVIEYVYRIIQPIAEENEVDLI--VECEDVV 153 Query: 250 IFVDSDKIARAIDNLLMNALKY-SVKPGA---IHVRMITDPKQAAIEVENRGIPLTREQQ 305 + + +++ + + NL+ NA+KY S+K + VR P +EVE+ G + +E Q Sbjct: 154 VRGNKERLIQMLLNLVDNAVKYTSLKEKGEKKVWVRAYDTPDWVVVEVEDTGPGIPKEAQ 213 Query: 306 EKLFERFYKVDHSRSSEGIQTGAGLGLSIARNIAERHGGTLTLD-HVNDVFTFRLTIP 362 ++FE+FY+VD +RS + G GLGL+I + I ++HGG + ++ +N R+ +P Sbjct: 214 SRIFEKFYRVDKARSRK--MGGTGLGLTIVKTIVDKHGGKIEVESEINQGTLMRVLLP 269 >Cgl2555 [T] COG0642 Signal transduction histidine kinase Length = 485 Score = 115 bits (289), Expect = 8e-26, Method: Compositional matrix adjust. Identities = 88/304 (28%), Positives = 145/304 (47%), Gaps = 10/304 (3%) Query: 61 FITPFFLLVFTITFFGLTRRIVKDLMTLERGLQMITEGDLNHRVP-VHRQDELGRVAYNI 119 I L+ IT L RR ++ L +E I GDL+ RVP E+G+++ + Sbjct: 187 IIGALILVAILITSLFLVRRSLRPLREVEETATRIAGGDLDRRVPQWPMTTEVGQLSNAL 246 Query: 120 NQMTERLQLQIAKERENERSKMELITGISHDLRTPLTSIIGYIELLRTKSFQDQEEYTRF 179 N M E+LQ I ++ E + SH+LRTPLTS+ G+ EL + + D Sbjct: 247 NIMLEQLQASILTAQQKEAQMRRFVGDASHELRTPLTSVKGFTELYSSGATDDA---NWV 303 Query: 180 VQNTYNKSIHLKKLLDDLFEYTRLNSIDTRQNVHRIDXXXXXXXXXXXXXPIAHDNAIHI 239 + ++ + L++DL TR + HR+D D +++ Sbjct: 304 MSKIGGEAQRMSVLVEDLLSLTRAEGQQMEK--HRVDVLELALAVRGSMRAAWPDRTVNV 361 Query: 240 IKDIGSSPVLIFVDSDKIARAIDNLLMNALKYSVKPGAIHVRMITDPKQAAIEVENRGIP 299 S PV + D ++ + + NL+ N L + + + + TD + I V + G+ Sbjct: 362 SNKAESIPV-VKGDPTRLHQVLTNLVANGLNHGGPDAEVSIEINTDGQNVRILVADNGVG 420 Query: 300 LTREQQEKLFERFYKVDHSRSSEGIQTGAGLGLSIARNIAERHGGTLTLDHVNDVFT-FR 358 ++ E + +FERFY+ D SRS G+GLGL+I +++ E HGGT+T+D V T F Sbjct: 421 MSEEDAQHIFERFYRADSSRSRA--SGGSGLGLAITKSLVEGHGGTVTVDSVQGEGTVFT 478 Query: 359 LTIP 362 +T+P Sbjct: 479 ITLP 482 >MT3343 [T] COG0642 Signal transduction histidine kinase Length = 567 Score = 115 bits (287), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 80/289 (27%), Positives = 144/289 (49%), Gaps = 6/289 (2%) Query: 77 LTRRIVKDLMTLERGLQMITEGDLNHRVPVHRQDELGRVAYNINQMTERLQLQIAKEREN 136 ++R++V + + R + EG L+ R+PV +D++ R+A + N M E L QIA+ E Sbjct: 233 VSRQVVVPVRSASRIAERFAEGHLSERMPVRGEDDMARLAVSFNDMAESLSRQIAQLEEF 292 Query: 137 ERSKMELITGISHDLRTPLTSIIGYIELLRTKSFQDQEEYTRFVQNTYNKSIHLKKLLDD 196 + + +SH+LRTPLT++ +L+ S R + ++ + LL+D Sbjct: 293 GNLQRRFTSDVSHELRTPLTTVRMAADLIYDHSADLDPTLRRSTELMVSELDRFETLLND 352 Query: 197 LFEYTRLNSIDTRQNVHRIDXXXXXXXXXXXXXPIAHDNAIHIIKDIGSSPVLIFVDSDK 256 L E +R ++ +V +D +A + I ++ D+ + V+ VD+ + Sbjct: 353 LLEISRHDAGVAELSVEAVDLRTTVNNALGNVGHLAEEAGIELLVDLPAEQVIAEVDARR 412 Query: 257 IARAIDNLLMNALKYSV-KPGAIHVRMITDPKQAAIEVENRGIPLTREQQEKLFERFYKV 315 + R + NL+ NA+ ++ KP + +RM D A+ V + G+ L +++ +F RF++ Sbjct: 413 VERILRNLIANAIDHAEHKP--VRIRMAADEDTVAVTVRDYGVGLRPGEEKLVFSRFWRS 470 Query: 316 DHSRSSEGIQTGAGLGLSIARNIAERHGGTL-TLDHVNDVFTFRLTIPI 363 D SR G GLGL+I+ A H G L + FRLT+P+ Sbjct: 471 DPSRVRR--SGGTGLGLAISVEDARLHQGRLEAWGEPGEGACFRLTLPL 517 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.139 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,801,859 Number of extensions: 884922 Number of successful extensions: 3641 Number of sequences better than 1.0e-05: 193 Number of HSP's gapped: 3349 Number of HSP's successfully gapped: 198 Length of query: 377 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 269 Effective length of database: 25,922,359 Effective search space: 6973114571 Effective search space used: 6973114571 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 117 (49.7 bits)