BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0544.0015.p (498 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC0528 [R] COG0488 ATPase components of ABC transporters with d... 682 0.0 L119564 [R] COG0488 ATPase components of ABC transporters with d... 355 1e-97 BH1900 [R] COG0488 ATPase components of ABC transporters with du... 171 2e-42 L73239 [R] COG0488 ATPase components of ABC transporters with du... 171 3e-42 BH2025 [R] COG0488 ATPase components of ABC transporters with du... 160 4e-39 >CAC0528 [R] COG0488 ATPase components of ABC transporters with duplicated ATPase domains Length = 492 Score = 682 bits (1761), Expect = 0.0, Method: Compositional matrix adjust. Identities = 338/498 (67%), Positives = 400/498 (80%), Gaps = 6/498 (1%) Query: 1 MSLINVTGLTFAYEGTYDNIFENVNXXXXIDTDWKLGFTGRNGRGKTTFLQLLLGKHEYS 60 MSLINVT LTFAYEG+YDNIFENV+ IDTDWKLGFTGRNGRGKTTFL LLLGK+EY+ Sbjct: 1 MSLINVTNLTFAYEGSYDNIFENVSFQ--IDTDWKLGFTGRNGRGKTTFLNLLLGKYEYN 58 Query: 61 GTISAKVNFXXXXXYFPYPVPNKEYHTLDVISDMFPDYVHWQFMRELSLLQVDEDVLYRP 120 G+IS VNF YFPY V K +DVI ++ P+ + W+ ++ELSLL +D + LYR Sbjct: 59 GSISTDVNFE----YFPYEVQEKGKFVIDVIKEISPNSMDWEIVKELSLLDIDYEALYRQ 114 Query: 121 FASLSNGEQTKVLLAALFIKENSFLLIDEPTNHLDMHARTLVSEYLNTKSGYILVSHDRS 180 F +LS GEQTK LLAA+F+KENSFLLIDEPTNHLD R +S YL K G+IL+SHDRS Sbjct: 115 FYTLSKGEQTKSLLAAMFLKENSFLLIDEPTNHLDAEGRKKLSNYLKKKKGFILISHDRS 174 Query: 181 FLDHCVDHILSINKTNIEIQKGNFSSWWENKRRQDNFELAQNEKLRKDIKRLSDSSKRTG 240 FLD+CVDHILSIN+TNIEIQKGNFSSWW NK +D FELA+NEKL+KDI RLS S+KRT Sbjct: 175 FLDNCVDHILSINRTNIEIQKGNFSSWWRNKELKDGFELAENEKLKKDINRLSSSAKRTS 234 Query: 241 NWSHEVEKSKNGTRNSGSKIDKGYVGHKAAKMMKRSKSIEQRQQSAITEKSKLLKNIESS 300 WS VE SK GT NSGSK+DKGYVGHK+ KMMKR+K+IE RQQ+ I EKS+LLKNIE++ Sbjct: 235 TWSDSVESSKYGTTNSGSKLDKGYVGHKSEKMMKRAKNIEARQQNMIKEKSRLLKNIENN 294 Query: 301 DTLEITQLAYHKNILAELEQVSIFYGEKQVCANVNFTIEQGERIALSGKNGSGKSSILKL 360 + L+I L +H L E+ V+I Y ++ VC V F IE+GERIA+ GKNGSGKSSILKL Sbjct: 295 ENLKIVPLTFHDKKLVEVIDVAIKYDDRIVCEGVRFNIEKGERIAIQGKNGSGKSSILKL 354 Query: 361 ICGEVIPYTGSFMKDSQLKISYVSQDTSHLQGSLADYIRDNGIDETLFKSILRKLDFSRN 420 I GE IP++G K+++L ISYVSQDTS L G+L++Y IDETLFKS+LRKLDFSR Sbjct: 355 IYGEDIPHSGIVRKNNKLIISYVSQDTSDLYGNLSEYADKYCIDETLFKSMLRKLDFSRE 414 Query: 421 QFEKDLSTYSGGQKKKVLIAKSLSEQVHLHIWDEPLNFIDVISRMQIEELLLEYTPTILF 480 QFEKD+ +SGGQKKKVL+AKSL E+ HL+IWDEPLNFIDVISRMQ+E+LL+EY PTILF Sbjct: 415 QFEKDIKDFSGGQKKKVLLAKSLCERAHLYIWDEPLNFIDVISRMQVEKLLIEYEPTILF 474 Query: 481 VEHDREFCSNVATKTIEL 498 VEHD FC NVATKTI+L Sbjct: 475 VEHDISFCENVATKTIKL 492 >L119564 [R] COG0488 ATPase components of ABC transporters with duplicated ATPase domains Length = 60 Score = 355 bits (910), Expect = 1e-97, Method: Compositional matrix adjust. Identities = 0/529 (0%), Positives = 0/529 (0%), Gaps = 50/529 (9%) Query: 1 MSLINVTGLTFAYEGTYDNIFENVNXXXXIDTDWKLGFTGRNGRGKTTFLQLLLGKHEYS 60 M + G + + + + + Sbjct: 1 MKNPPLAGHLKKSS---YRKMMD-KITNIHWLSKEDVGFEPTHAFTCLTVFKTVPFSQAW 56 Query: 61 GTISAKVNFXXXXXYFPYPVPNKEYHTLD-----------------------------VI 91 Sbjct: 57 ---- 112 Query: 92 SDMFPDYVHWQFMRELSLLQVDEDVLYRPFASLSNGEQTKVLLAALFIKENSFLLIDEPT 151 Sbjct: 113 172 Query: 152 NHLDMHARTLVSEYLNTKSGYILVSHDRSFLDHCVDHILSINKTNIEIQKGNFSSWWENK 211 Sbjct: 173 232 Query: 212 RRQDNFELAQNEKLRKDIKRLSDSSKRTGNWSHEVEKSKNGTRNSGSKIDKGYVGHKAAK 271 Sbjct: 233 ---------- 282 Query: 272 MM-KRSKSIEQRQQSAITEKSKLLKNIESSDTLEITQLAYHKNILAELEQVSIFYGEKQV 330 Sbjct: 283 342 Query: 331 CANVNFTIEQGERIALSGKNGSGKSSILKLICGEVIPYTGSFMKDSQ-LKISYVSQDTSH 389 Sbjct: 343 402 Query: 390 LQGSLADYIRDNGIDETLFKSILRKLDFSRNQFEKDLSTYSGGQKKKVLIAKSLSEQVHL 449 Sbjct: 403 - 461 Query: 450 HIWDEPLNFIDVISRMQIEELLLEYTPTILFVEHDREFCSNVATKTIEL 498 Sbjct: 462 510 >BH1900 [R] COG0488 ATPase components of ABC transporters with duplicated ATPase domains Length = 538 Score = 171 bits (434), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 127/470 (27%), Positives = 230/470 (48%), Gaps = 35/470 (7%) Query: 35 KLGFTGRNGRGKTTFLQLLLGKHEYSGTISAKVNFXXXXXYFPYPVPNKEYHTLDVISDM 94 ++G G+NG GK+T L++L G G S V Y P + D D Sbjct: 31 RIGVVGKNGEGKSTLLKMLAGVLASDG--SGFVKAYGDIAYIP------QLEDRDASLD- 81 Query: 95 FPDYVHWQFMRELSLLQVDEDVLYRPFASLSNGEQTKVLLAALFIKENSFLLIDEPTNHL 154 + ++ ++ Q + +S GEQT+ +A L+ DEPT+HL Sbjct: 82 ------GRVKKQWNVSQASNE-------RMSGGEQTRKKIAQALSTSAKILIADEPTSHL 128 Query: 155 DMHA-RTLVSEYLNTKSGYILVSHDRSFLDHCVDHILSINKTNIEIQKGNFSSWWENK-- 211 D+ L +E ++ IL+SHDR L+ + + + GN ++ + K Sbjct: 129 DLKGIEKLEAELIHFPGALILISHDRELLNRVCTSVWEVESGEVNQYDGNVDAYLQQKEH 188 Query: 212 -RRQDNFELAQNEKLRKDIKRLSDSSKRTGNWSHEVEKSKNGTRNSGSKIDKGYVGHKAA 270 R + FE Q +K+ KRL +++++ S + K+ NS +++ K VG K A Sbjct: 189 ERERQQFEYVQ---FQKEKKRLEEAARKQSETSKSLRKAPKRMGNSEARLHKRSVGQKKA 245 Query: 271 KMMKRSKSIEQR-QQSAITEKSKLLKNIESSDTLEITQLAYHKNILAELEQVSIFYGEKQ 329 K+ R+K+I R +Q + EK K K I+ ++ Q + K +L+ E V+I GE Sbjct: 246 KLDNRAKAIRSRIEQLDVKEKPKEAKPIQFD--VQTFQAMHSKTVLS-FENVTIQVGESL 302 Query: 330 VCANVNFTIEQGERIALSGKNGSGKSSILKLICGEVIPYTGSFMKDSQLKISYVSQDTSH 389 + + ++ +I+ G+++A++G NG+GK+++L LI P + + L + + Sbjct: 303 LVSRISGSIQPGDKLAITGPNGAGKTTLLHLIEKANAPGI-KLARPAVLATFHQRLEQLD 361 Query: 390 LQGSLADYIRDN-GIDETLFKSILRKLDFSRNQFEKDLSTYSGGQKKKVLIAKSLSEQVH 448 + S+ + + D+ +ET +++L +L F + K + SGG++ KV +A L Q + Sbjct: 362 EERSILENVSDSSAYEETFVRTVLARLLFREEEVHKKVGVLSGGERMKVALANVLLSQAN 421 Query: 449 LHIWDEPLNFIDVISRMQIEELLLEYTPTILFVEHDREFCSNVATKTIEL 498 + + DEP N++D+ SR + ++L Y T++FV HDR ++AT + L Sbjct: 422 VLLLDEPTNYLDLQSRQALVDVLQAYPGTVVFVTHDRHLLQSIATHVLPL 471 Score = 77.8 bits (190), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 54/185 (29%), Positives = 96/185 (51%), Gaps = 19/185 (10%) Query: 314 ILAELEQVSIFYGEKQVCANVNFTIEQGERIALSGKNGSGKSSILKLICGEVIPYTGSFM 373 +L E +Q+ YG++QV + +G+RI + GKNG GKS++LK++ G V+ GS Sbjct: 2 LLLEAKQLEKTYGDRQVIQAEELFVYEGDRIGVVGKNGEGKSTLLKMLAG-VLASDGSGF 60 Query: 374 KDSQLKISYVSQDTSHLQGSLADYIRDNGIDETLFKSILRKLDFSRNQFEKDLSTYSGGQ 433 + I+Y+ Q L D RD +D + ++ + S+ E+ SGG+ Sbjct: 61 VKAYGDIAYIPQ--------LED--RDASLD----GRVKKQWNVSQASNER----MSGGE 102 Query: 434 KKKVLIAKSLSEQVHLHIWDEPLNFIDVISRMQIEELLLEYTPTILFVEHDREFCSNVAT 493 + + IA++LS + I DEP + +D+ ++E L+ + ++ + HDRE + V T Sbjct: 103 QTRKKIAQALSTSAKILIADEPTSHLDLKGIEKLEAELIHFPGALILISHDRELLNRVCT 162 Query: 494 KTIEL 498 E+ Sbjct: 163 SVWEV 167 Score = 74.7 bits (182), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 53/167 (31%), Positives = 83/167 (49%), Gaps = 10/167 (5%) Query: 35 KLGFTGRNGRGKTTFLQLLLGKHEYSGTISAKVNFXXXXXYFPYPVP--NKEYHTLDVIS 92 KL TG NG GKTT L L+ E + K+ F + ++E L+ +S Sbjct: 316 KLAITGPNGAGKTTLLHLI----EKANAPGIKLARPAVLATFHQRLEQLDEERSILENVS 371 Query: 93 DMFPDYVHWQFMRE-LSLLQVDEDVLYRPFASLSNGEQTKVLLAALFIKENSFLLIDEPT 151 D F+R L+ L E+ +++ LS GE+ KV LA + + + + LL+DEPT Sbjct: 372 D--SSAYEETFVRTVLARLLFREEEVHKKVGVLSGGERMKVALANVLLSQANVLLLDEPT 429 Query: 152 NHLDMHARTLVSEYLNTKSGYIL-VSHDRSFLDHCVDHILSINKTNI 197 N+LD+ +R + + L G ++ V+HDR L H+L + K I Sbjct: 430 NYLDLQSRQALVDVLQAYPGTVVFVTHDRHLLQSIATHVLPLEKGVI 476 >L73239 [R] COG0488 ATPase components of ABC transporters with duplicated ATPase domains Length = 635 Score = 171 bits (432), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 140/530 (26%), Positives = 246/530 (46%), Gaps = 81/530 (15%) Query: 18 DNIFENVNXXXXIDTDWKLGFTGRNGRGKTTFLQLLLGKHEYS-GTIS------------ 64 D +FEN+N + ++ GRNG GK+T L+++ G E S GT+S Sbjct: 16 DVLFENINFS--LQDHSRIALVGRNGAGKSTLLKIIAGVEEASRGTVSKTKDLTMNYLAQ 73 Query: 65 ------AKVNFXXXXXYFPY----------------PVPNKEYHTL----DVISDMFPDY 98 ++ F F + E L D +S+ F Sbjct: 74 DTGLNSSRTIFAEMLSVFDSLRQMEKQLRQMELQMGELAGDELTALMKRYDALSEEFRMK 133 Query: 99 VHWQFMRE----LSLLQVDEDVLYRPFASLSNGEQTKVLLAALFIKENSFLLIDEPTNHL 154 + + E L+ + D+ + R +SLS G++T++ LA + ++ L++DEPTNHL Sbjct: 134 NGFTYESEIKSVLNGFRFDDAMWEREISSLSGGQKTRLALAKMLLERPQLLILDEPTNHL 193 Query: 155 DMHARTLVSEYLNTKSGYIL-VSHDRSFLDHCVDHILSINKTNIEIQKGNFSSWWENKRR 213 D+ + YL G +L VSHDR FLD V+ +L +++ + GN+S + E + + Sbjct: 194 DIETLAWLENYLKNYEGSLLIVSHDRYFLDKVVNEVLELSRGELTRFSGNYSRYVEQREQ 253 Query: 214 QDNFELAQNEKLRKDIKRLSDSSKRTGNWSHEVEKSKNGTRNSGSKIDKGYVGHKAAKMM 273 + EL EK +K+I +L D R N R S +K+ + Sbjct: 254 KLLLELKNFEKQQKEIAKLEDYVAR------------NLVRASTTKMAQS---------- 291 Query: 274 KRSKSIEQRQQ-SAITEKSKLLKNIESSDTLEITQLAYHKNILAELEQVSIFYGEKQVCA 332 R K +E+ ++ A T K S + T N++ + + ++ Y + ++ Sbjct: 292 -RRKKLEKMERLDAPTSDDK-------SANVTFTPEKTSGNVVLTVSEATVGYEDNRLSG 343 Query: 333 NVNFTIEQGERIALSGKNGSGKSSILKLICGEVIPYTGSFMKDSQLKISYVSQDTSHLQG 392 +N +GE +A+ G NG GK++++K I G++ G + + + Y Q+ L Sbjct: 344 PINIDERKGESLAIVGPNGVGKTTLIKSIVGQIPFLAGQAKLGANVTLGYYDQEQGRLTA 403 Query: 393 S--LADYIRDNG--IDETLFKSILRKLDFSRNQFEKDLSTYSGGQKKKVLIAKSLSEQVH 448 S + D + + ++E +++L FS + +K + SGG+K ++L+AK + + Sbjct: 404 SNTVLDELWNEHRLLNEVEIRNMLGAFLFSGDDVKKTVGMLSGGEKARLLLAKLAMDHDN 463 Query: 449 LHIWDEPLNFIDVISRMQIEELLLEYTPTILFVEHDREFCSNVATKTIEL 498 + DEP N +D+ SR +E L+++ TILFV HDR F + VATK +E+ Sbjct: 464 FLVLDEPTNHLDIDSREVLENALIDFPGTILFVSHDRYFINRVATKVLEI 513 Score = 97.1 bits (240), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 69/231 (29%), Positives = 110/231 (47%), Gaps = 46/231 (19%) Query: 314 ILAELEQVSIFYGEKQVCANVNFTIEQGERIALSGKNGSGKSSILKLICGEVIPYTGSFM 373 IL + ++ +G+ + N+NF+++ RIAL G+NG+GKS++LK+I G G+ Sbjct: 2 ILLQGNGIARTFGDDVLFENINFSLQDHSRIALVGRNGAGKSTLLKIIAGVEEASRGTVS 61 Query: 374 KDSQLKISYVSQDTS----------------------------HLQ-GSLA--------- 395 K L ++Y++QDT LQ G LA Sbjct: 62 KTKDLTMNYLAQDTGLNSSRTIFAEMLSVFDSLRQMEKQLRQMELQMGELAGDELTALMK 121 Query: 396 -------DYIRDNGID-ETLFKSILRKLDFSRNQFEKDLSTYSGGQKKKVLIAKSLSEQV 447 ++ NG E+ KS+L F +E+++S+ SGGQK ++ +AK L E+ Sbjct: 122 RYDALSEEFRMKNGFTYESEIKSVLNGFRFDDAMWEREISSLSGGQKTRLALAKMLLERP 181 Query: 448 HLHIWDEPLNFIDVISRMQIEELLLEYTPTILFVEHDREFCSNVATKTIEL 498 L I DEP N +D+ + +E L Y ++L V HDR F V + +EL Sbjct: 182 QLLILDEPTNHLDIETLAWLENYLKNYEGSLLIVSHDRYFLDKVVNEVLEL 232 >BH2025 [R] COG0488 ATPase components of ABC transporters with duplicated ATPase domains Length = 539 Score = 160 bits (405), Expect = 4e-39, Method: Compositional matrix adjust. Identities = 135/508 (26%), Positives = 235/508 (46%), Gaps = 78/508 (15%) Query: 35 KLGFTGRNGRGKTTFLQLLLGKHEYS-GTISAKVNFXX-XXXYFPYPVPNKEYHTL--DV 90 ++G GRNG GK+T L ++ GK S GT++ K P P K L DV Sbjct: 31 RIGIIGRNGEGKSTLLHVMAGKEPPSEGTVTRKKGLSLGLLEQLPKLDPEKPVRGLLHDV 90 Query: 91 ISDM------------------------FPDY--VHWQFMRELSLLQVDEDV-------- 116 SD+ Y + QF+ +L ++D + Sbjct: 91 FSDLNELKGKLAEIERALAENPASLEQLLETYGSLQDQFL-QLGGYEIDSRINRVVSGLQ 149 Query: 117 ----LYRPFASLSNGEQTKVLLAALFIKENSFLLIDEPTNHLDMHARTLVSEYLNTKSG- 171 L + + LS GE+TK+ LA L ++ LL+DEPTNHLD+ A +++++ G Sbjct: 150 LVHLLDKKWQELSGGERTKIGLARLLLRSPELLLLDEPTNHLDLSAIEWLTDFIRQYDGT 209 Query: 172 YILVSHDRSFLDHCVDHILSINKTNIEIQKGNFSSWWENKRRQDNFELAQNEKLRKDIKR 231 ++VSHDR FLD I + + +GN+SS+ + + + E + +K IK+ Sbjct: 210 VVIVSHDRYFLDEVTTSIFELEHGELHTYQGNYSSYMKEREERILREFQHYQDQQKKIKK 269 Query: 232 LSDSSKRTGNWSHEVEKSKNGTRNSGSKIDKGYVGHKAAKMMKRSKSIEQRQQSAITEKS 291 + ++ KR W++ +G H+ AK M+++ + + + I + Sbjct: 270 MKETIKRLKEWANRSNPPNDGL-------------HRRAKSMEKALARIELLKKPILQHK 316 Query: 292 KL-----LKNIESSDTLEITQLAYHKNILAELEQVSIFYGEKQVCANVNFTIEQGERIAL 346 K+ ++N +D + +E V+ YGE+++ +V + ER+A+ Sbjct: 317 KVDLDFHIQNRSGTDVIM-------------MENVAKTYGERELFHDVQLHVRFQERVAI 363 Query: 347 SGKNGSGKSSILKLICGEVIPYTGSFMKDSQLKISYVSQDTSHLQGS---LADYIRDNGI 403 G+NGSGKS++L +I G+ P G S + I Y+SQ + G L ++ + Sbjct: 364 VGENGSGKSTLLNMILGKQQPEKGRIQIGSNVSIGYLSQHNEEMDGKKTILEEFRAKVTV 423 Query: 404 DETLFKSILRKLDFSRNQFEKDLSTYSGGQKKKVLIAKSLSEQVHLHIWDEPLNFIDVIS 463 E + IL + F N + + SGG++ ++ +A+ + ++ +L I DEP N +D+ S Sbjct: 424 TEGEARHILARYLFYGNTVFRKVKDLSGGERMRLRLAQLVHQRHNLLILDEPTNHLDIDS 483 Query: 464 RMQIEELLLEYTPTILFVEHDREFCSNV 491 R +EE L E+ T++ V HDR F + Sbjct: 484 REVLEEALSEFQGTVIAVSHDRYFLDRL 511 Score = 73.2 bits (178), Expect = 9e-13, Method: Compositional matrix adjust. Identities = 55/229 (24%), Positives = 97/229 (42%), Gaps = 44/229 (19%) Query: 314 ILAELEQVSIFYGEKQVCANVNFTIEQGERIALSGKNGSGKSSILKLICGEVIPYTGSFM 373 I+ L+ V YG V ++ +++GERI + G+NG GKS++L ++ G+ P G+ Sbjct: 2 IICSLQHVKQMYGAHTVFHDICCEVKKGERIGIIGRNGEGKSTLLHVMAGKEPPSEGTVT 61 Query: 374 KDSQLKISYVSQ-------------------DTSHLQGSLADYIRDNGIDETLFKSILRK 414 + L + + Q D + L+G LA+ R + + +L Sbjct: 62 RKKGLSLGLLEQLPKLDPEKPVRGLLHDVFSDLNELKGKLAEIERALAENPASLEQLLET 121 Query: 415 LDFSRNQF-------------------------EKDLSTYSGGQKKKVLIAKSLSEQVHL 449 ++QF +K SGG++ K+ +A+ L L Sbjct: 122 YGSLQDQFLQLGGYEIDSRINRVVSGLQLVHLLDKKWQELSGGERTKIGLARLLLRSPEL 181 Query: 450 HIWDEPLNFIDVISRMQIEELLLEYTPTILFVEHDREFCSNVATKTIEL 498 + DEP N +D+ + + + + +Y T++ V HDR F V T EL Sbjct: 182 LLLDEPTNHLDLSAIEWLTDFIRQYDGTVVIVSHDRYFLDEVTTSIFEL 230 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.133 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 34,955,380 Number of extensions: 1413162 Number of successful extensions: 21235 Number of sequences better than 1.0e-05: 725 Number of HSP's gapped: 20677 Number of HSP's successfully gapped: 1021 Length of query: 498 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 387 Effective length of database: 25,576,357 Effective search space: 9898050159 Effective search space used: 9898050159 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 118 (50.1 bits)