BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0544.0012.p (660 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_treP_2 [G] COG1263 Phosphotransferase system IIC components, ... 458 e-128 BH2216_2 [G] COG1263 Phosphotransferase system IIC components, g... 438 e-122 SA0432_2 [G] COG1263 Phosphotransferase system IIC components, g... 320 6e-87 VCA0653_2 [G] COG1263 Phosphotransferase system IIC components, ... 307 5e-83 PM1846_2 [G] COG1263 Phosphotransferase system IIC components, g... 300 6e-81 >BS_treP_2 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 379 Score = 458 bits (1178), Expect = e-128, Method: Compositional matrix adjust. Identities = 223/379 (58%), Positives = 281/379 (74%), Gaps = 1/379 (0%) Query: 92 VKTAASKKQNPLQQAIKTLADIFIPILPAIVMAXXXXXXXXXXTGPGIFFDSKSLVEVYP 151 VK A+ K NPLQ+A+KTLADIFIPILPAIV A T GIFF +KS+V+VYP Sbjct: 1 VKKASEKNMNPLQRAVKTLADIFIPILPAIVTAGLLMGINNILTAEGIFFSTKSIVQVYP 60 Query: 152 QWKDFASIINTIASTAFTFLPALIGWSAVTRFGGSPXXXXXXXXXXXHPDLLSAYGYADA 211 QW D A++IN IA TAFTFLPALIGWSAV RFGG+P HPDLL+A+GY A Sbjct: 61 QWADLANMINLIAGTAFTFLPALIGWSAVKRFGGNPLLGIVLGVMLVHPDLLNAWGYGAA 120 Query: 212 KVEGTVPVWDLFGWHVNKIGYQGQVLPVLVSAYLLARLERWLNQKVPDSFKLLIVAPVAL 271 + G +PVW+LFG V K+GYQGQVLP+L+++Y+LA++E +L ++ P+ +LL+VAP+ L Sbjct: 121 EQSGEIPVWNLFGLEVQKVGYQGQVLPILLASYMLAKIEVFLTKRTPEGIQLLVVAPITL 180 Query: 272 LITGFLAFTVIGPITFVIANAITSGLVSIFGGFPXXXXXXXXXXXXXXVITGMHHTFLAV 331 L+TGF +F +IGPITF I N +TSGL+S+FG F VITGMHHTFLAV Sbjct: 181 LLTGFASFIIIGPITFAIGNVLTSGLISVFGSFAALGGLLYGGFYSALVITGMHHTFLAV 240 Query: 332 DIQLIGSE-GGTFLWPMLALSNIAQGAAALGMMFVAKQEKTKGLAVTSSISAFLGVTEPA 390 D+QLIGS+ GGTFLWPMLALSNIAQG+AAL MMF+ K EK KGL++TS ISA+LG+TEPA Sbjct: 241 DLQLIGSKLGGTFLWPMLALSNIAQGSAALAMMFIVKDEKQKGLSLTSGISAYLGITEPA 300 Query: 391 IFGVNVRFKYPFIFGMLGSAIAGVLLAMNNVLASSIGVGGIPGFLSIFPSQWGVFFIGMA 450 IFGVN+R+++PFI M+ S +AG+ ++ VLASS+GVGG+PG SI WG F IGMA Sbjct: 301 IFGVNLRYRFPFIIAMVSSGLAGMYISSQGVLASSVGVGGVPGIFSIMSQYWGAFAIGMA 360 Query: 451 IVLIVPFTATVLFGKVRSR 469 IVLIVPF T + + + + Sbjct: 361 IVLIVPFAGTYAYARFKHK 379 >BH2216_2 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 379 Score = 438 bits (1126), Expect = e-122, Method: Compositional matrix adjust. Identities = 213/376 (56%), Positives = 273/376 (72%) Query: 92 VKTAASKKQNPLQQAIKTLADIFIPILPAIVMAXXXXXXXXXXTGPGIFFDSKSLVEVYP 151 VK AA+K N Q+A+KTLADIFIPILPAIV A T GIF+D S+++++P Sbjct: 1 VKDAAAKNTNVFQRAVKTLADIFIPILPAIVTAGLLMGINNILTAEGIFYDGASVIDIHP 60 Query: 152 QWKDFASIINTIASTAFTFLPALIGWSAVTRFGGSPXXXXXXXXXXXHPDLLSAYGYADA 211 QW DFA IIN IA+TAF FLP LIGWSAV RFGGS HPDLL+A+GY A Sbjct: 61 QWADFAGIINLIANTAFVFLPGLIGWSAVKRFGGSELLGIVLGLMLVHPDLLNAWGYGQA 120 Query: 212 KVEGTVPVWDLFGWHVNKIGYQGQVLPVLVSAYLLARLERWLNQKVPDSFKLLIVAPVAL 271 ++EG +P W+LFG + ++GYQGQVLP+L SA++LA++E +L ++VPDS +LL+VAPVAL Sbjct: 121 QLEGEIPTWNLFGLTIEQVGYQGQVLPILFSAWILAKIEIFLRKRVPDSIQLLVVAPVAL 180 Query: 272 LITGFLAFTVIGPITFVIANAITSGLVSIFGGFPXXXXXXXXXXXXXXVITGMHHTFLAV 331 LITGF+AF IGPITF I N IT+ SIF P V+TGMHHTFLAV Sbjct: 181 LITGFIAFAAIGPITFTIGNGITNVFTSIFAAVPLVGGFLYGLIYAPLVVTGMHHTFLAV 240 Query: 332 DIQLIGSEGGTFLWPMLALSNIAQGAAALGMMFVAKQEKTKGLAVTSSISAFLGVTEPAI 391 D+QLIG+ GGTFLWP+L LSNIAQG+AAL MMF + EK KGL+++S++SA+LG+TEPA+ Sbjct: 241 DLQLIGTIGGTFLWPILVLSNIAQGSAALAMMFATRDEKLKGLSLSSAVSAYLGITEPAM 300 Query: 392 FGVNVRFKYPFIFGMLGSAIAGVLLAMNNVLASSIGVGGIPGFLSIFPSQWGVFFIGMAI 451 FGVN+R+K+PF+ ++ +AI G + +N VLA+SIGVGG+PG SI WGVFFIGM I Sbjct: 301 FGVNIRYKFPFVCAIISAAIGGAFITVNGVLANSIGVGGLPGIFSIQAGFWGVFFIGMVI 360 Query: 452 VLIVPFTATVLFGKVR 467 I+P T L+ ++R Sbjct: 361 AFILPLVLTYLYARIR 376 >SA0432_2 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 372 Score = 320 bits (819), Expect = 6e-87, Method: Compositional matrix adjust. Identities = 186/373 (49%), Positives = 242/373 (64%), Gaps = 13/373 (3%) Query: 104 QQAIKTLADIFIPILPAIVMAXXXXXXXXXXTGPGIFFDSKSLVEVYPQWKDFASIINTI 163 Q+ IK L DIFIPILPAIV A T G+F K+L+E+YPQ D ++IIN I Sbjct: 1 QRLIKLLGDIFIPILPAIVTAGLLMGINNLLTMKGLF-GPKALIEMYPQIADISNIINVI 59 Query: 164 ASTAFTFLPALIGWSAVTRFGGSPXXXXXXXXXXXHPDLLSAYGYADAKVEGTVPVWDLF 223 ASTAF FLPALIGWS++ FGGSP HP L+S Y A +G +P W+LF Sbjct: 60 ASTAFIFLPALIGWSSMRVFGGSPILGIVLGLILMHPQLVSQYDLA----KGNIPTWNLF 115 Query: 224 GWHVNKIGYQGQVLPVLVSAYLLARLERWLNQKVPDSFKLLIVAPVALLITGFLAFTVIG 283 G + ++ YQGQVLPVL++AY+LA++E+ LN+ V DS K+L+V PVALL+TGFLAF +IG Sbjct: 116 GLEIKQLNYQGQVLPVLIAAYVLAKIEKGLNKVVHDSIKMLVVGPVALLVTGFLAFIIIG 175 Query: 284 PITFVIANAITSGLVSIFGGFPXXXXXXXXXXXXXXVITGMHHTFLAVDIQLIGSE-GGT 342 P+ +I ITSG+ IF VITG+HH FLAVD QL+GS GGT Sbjct: 176 PVALLIGTGITSGVTFIFQHAGWLGGAIYGLLYAPLVITGLHHMFLAVDFQLMGSSLGGT 235 Query: 343 FLWPMLALSNIAQGAAALGMMFVAKQEKT---KGLAVTSSISAFLGVTEPAIFGVNVRFK 399 +LWP++A+SNI QG+AA G FV K+ K +GLA+TS ISA LGVTEPA+FGVN+ K Sbjct: 236 YLWPIVAISNICQGSAAFGAWFVYKRRKMVKEEGLALTSCISAMLGVTEPAMFGVNLPLK 295 Query: 400 YPFIFGMLGSAIAGVLLAMNNVLASSIGVGGIPGFLSIFPSQWGVFFIGMAIVLIVPFTA 459 YPFI + S + G ++ MNNVL VGG+P F+SI W V+ I AI ++VP Sbjct: 296 YPFIAAISTSCVLGAIVGMNNVLGKVG-VGGVPAFISIQKEFWPVYLIVTAIAIVVPCIL 354 Query: 460 TVL---FGKVRSR 469 T++ F K +++ Sbjct: 355 TIVMSHFSKQKAK 367 >VCA0653_2 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 386 Score = 307 bits (786), Expect = 5e-83, Method: Compositional matrix adjust. Identities = 164/383 (42%), Positives = 242/383 (63%), Gaps = 25/383 (6%) Query: 95 AASKKQNPLQQAIKTLADIFIPILPAIVMAXXXXXXXXXXTGPGIFFDSKSLVEVYPQWK 154 A ++KQN Q+A+K L+DIF+ I+PAIV T PG+F + +SL++ P Sbjct: 1 AGAEKQNWAQRAVKGLSDIFVRIIPAIVAGGLLMGLFNVLTAPGLFIEGQSLIDANPGLA 60 Query: 155 DFASIINTIASTAFTFLPALIGWSAVTRFGGSPXXXXXXXXXXXHPDLLSAYGYADAKVE 214 D AS+INT A+ F +LP L+ +SA +FGG+P HPDLL+ +G+ A V Sbjct: 61 DLASMINTFANAPFVYLPVLLAFSASRKFGGNPFLGAALGMLMVHPDLLNGWGFGSASVS 120 Query: 215 GTVPVWDLFGWHVNKIGYQGQVLPVLVSAYLLARLERWLNQKVPDSFKLLIVAPVALLIT 274 GTVP W++ G+ + K+GYQG VLPVLVSAY+LA++E L + VP L+ +A+ IT Sbjct: 121 GTVPTWNILGFEIEKVGYQGSVLPVLVSAYILAKIENGLRKIVPSVVDNLLTPMLAIFIT 180 Query: 275 GFLAFTVIGPIT----FVIANAI-----TSGLV--SIFGGFPXXXXXXXXXXXXXXVITG 323 GFL FTV+GP+T F++ +A+ ++G V ++FG VITG Sbjct: 181 GFLTFTVVGPLTRDVGFMLGDALNWLYDSAGFVGGALFG-----------FIYAPFVITG 229 Query: 324 MHHTFLAVDIQLIG---SEGGTFLWPMLALSNIAQGAAALGMMFVAKQEKTKGLAVTSSI 380 MHH+F+A++ QL+ + GGTF++P+ A+SNIAQGAAAL + + K+ K KG+A+ S + Sbjct: 230 MHHSFIAIETQLLADIVTTGGTFIFPIAAMSNIAQGAAALAVGVMTKETKLKGVAIPSGV 289 Query: 381 SAFLGVTEPAIFGVNVRFKYPFIFGMLGSAIAGVLLAMNNVLASSIGVGGIPGFLSIFPS 440 +A LG+TEPA+FGVN++ +YPFI + G+A+A + + NV A ++G G+PG +SI P Sbjct: 290 TALLGITEPAMFGVNLKLRYPFIAAICGAALASAFITLFNVKAQALGAAGLPGIISINPQ 349 Query: 441 QWGVFFIGMAIVLIVPFTATVLF 463 Q G + +GMAI + F TVL Sbjct: 350 QIGYYIMGMAISFVAAFALTVLL 372 >PM1846_2 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 384 Score = 300 bits (767), Expect = 6e-81, Method: Compositional matrix adjust. Identities = 163/377 (43%), Positives = 227/377 (60%), Gaps = 3/377 (0%) Query: 92 VKTAASKKQNPLQQAIKTLADIFIPILPAIVMAXXXXXXXXXXTGPGIFFDSKSLVEVYP 151 V A ++KQ LQ+ +K LADIF+PI+PAIV T G F + K++V++YP Sbjct: 1 VAAAGTEKQGLLQRLVKGLADIFVPIIPAIVAGGLLMGIHSMLTAKGFFVEEKNVVDLYP 60 Query: 152 QWKDFASIINTIASTAFTFLPALIGWSAVTRFGGSPXXXXXXXXXXXHPDLLSAYGYADA 211 D INTIA+ F FLP L+G+SA +FGG+P HP L + YA Sbjct: 61 AIADLVDFINTIANAPFVFLPVLLGFSATRKFGGNPFLGAALGMLLVHPALSDGWNYALT 120 Query: 212 KVEGTVPVWDLFGWHVNKIGYQGQVLPVLVSAYLLARLERWLNQKVPDSFKLLIVAPVAL 271 +G + W +FG + ++GYQG V+PVLV++++LA LE+ L + VP LI AL Sbjct: 121 LAKGNIQYWHIFGLEIERVGYQGTVIPVLVASWVLATLEKNLRKVVPSFLDNLITPLFAL 180 Query: 272 LITGFLAFTVIGPITFVIANAITSGLVSIFGGFPXXXXXXXXXXXXXXVITGMHHTFLAV 331 ITG LAFTVIGPI + I++GL ++ VITGMH TF+AV Sbjct: 181 FITGLLAFTVIGPIGREAGSLISTGLTWLYDTLGFVGGAIFGTLYAPIVITGMHQTFIAV 240 Query: 332 DIQL---IGSEGGTFLWPMLALSNIAQGAAALGMMFVAKQEKTKGLAVTSSISAFLGVTE 388 + QL + GGTF++P+ A+SNIAQGAA LG +V K K +G+AV S ISA LG+TE Sbjct: 241 ETQLLAEVARTGGTFIFPIAAMSNIAQGAACLGAAYVMKDAKVRGIAVPSGISALLGITE 300 Query: 389 PAIFGVNVRFKYPFIFGMLGSAIAGVLLAMNNVLASSIGVGGIPGFLSIFPSQWGVFFIG 448 PA+FGVN+R++YPFI M+G+ I+ ++A+ NV A ++G G+PG SI P ++ +G Sbjct: 301 PAMFGVNLRYRYPFISAMIGAGISSAVIALFNVKAIALGAAGLPGIPSIKPDSLAMYCVG 360 Query: 449 MAIVLIVPFTATVLFGK 465 M I + FT TV+ GK Sbjct: 361 MLISASIAFTLTVILGK 377 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.137 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 43,789,783 Number of extensions: 1701712 Number of successful extensions: 5807 Number of sequences better than 1.0e-05: 143 Number of HSP's gapped: 5658 Number of HSP's successfully gapped: 143 Length of query: 660 Length of database: 38,378,431 Length adjustment: 113 Effective length of query: 547 Effective length of database: 25,345,689 Effective search space: 13864091883 Effective search space used: 13864091883 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 119 (50.4 bits)