BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0544.0011.p (378 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_treA [G] COG0366 Glycosidases 421 e-117 BH0872 [G] COG0366 Glycosidases 412 e-115 VC0911 [G] COG0366 Glycosidases 385 e-107 STM4453 [G] COG0366 Glycosidases 356 4e-98 YPO3696 [G] COG0366 Glycosidases 355 5e-98 >BS_treA [G] COG0366 Glycosidases Length = 565 Score = 421 bits (1082), Expect = e-117, Method: Compositional matrix adjust. Identities = 15/375 (4%), Positives = 62/375 (16%), Gaps = 8/375 (2%) Query: 1 MIFWAEKGVDGFRMDVINLISKDQSFXXXXXXXXXXXXRRFYTDGPRVHEYIKELYAKVF 60 + + + + Y Sbjct: 189 DTYGHIPNGNRSYYLSRSQPPFFALMVELLAQHE----GDAALKQYLPQMQKEYAYWMDG 244 Query: 61 GPYNLVTVGEMSSTTLEHCIRYSAPEEREFSMTFNFHHLKVDYKNGQKWELQPYDFAELK 120 E L+ + + + + + +L+ Sbjct: 245 VENLQAGQQEKRVVKLQDGTLLNRYWDDRDTPRPESWVEDIATAKSNPNRPATEIYRDLR 304 Query: 121 ELFTRWQTGMQQAGGWNALFWNNHDQPRALSRFADDGKYRKESAKMLATTLHGMQGTPYV 180 + ++ + Sbjct: 305 SAAASGWDFSSRWMDNPQQLNTLRTTSIVPVDLNSLMFKMEKILARASKAAGDNAMANQY 364 Query: 181 YQGEELGMPNPDWHDISEFNDIESKNMYRILQERGLSGEQALDIIRERSRDNSRTPMQWD 240 + + ++ + + + R AL + + R Sbjct: 365 ETLANARQKGIEKYLWNDQQGWYADYDLKSHKVRNQLTAAALFPLYVNAAAKDRANKMAT 424 Query: 241 ASGHAGFTSGTPWLKVDRRYKEINVQAEQIDPDSIFAHYKQLIRLRKQEPVITDGIFKRL 300 A+ G + ++ + + Q ++ I+ + Sbjct: 425 ATKTHLLQPGGLNTTSVKSGQQWDAPNGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNV 484 Query: 301 DEGHPQIFAYARVGNTETLIVVSSFSGTQAGFSFADEFWSSRINSQAAELLIGNTEQTPV 360 + + + T F ++ + + +L+ + Sbjct: 485 QHTYDREKKLVEKYDVSTTGTGGGGGEYPLQDGFGW---TNGVTLKMLDLICPKEQPCDN 541 Query: 361 WSQSLEIPPYGSYMW 375 + + Sbjct: 542 V-PATRPTVKSATTQ 555 >BH0872 [G] COG0366 Glycosidases Length = 559 Score = 412 bits (1060), Expect = e-115, Method: Compositional matrix adjust. Identities = 197/378 (52%), Positives = 259/378 (68%), Gaps = 3/378 (0%) Query: 1 MIFWAEKGVDGFRMDVINLISKDQSFXXXXXXXXXXXXRRFYTDGPRVHEYIKELYAKVF 60 M FW KGVDGFR+DVINLISKDQ+F RRFYTDGPR HE++ E+ KVF Sbjct: 185 MDFWFTKGVDGFRLDVINLISKDQAFPNDDGSVPPGDGRRFYTDGPRAHEFMNEMNRKVF 244 Query: 61 GPYNLVTVGEMSSTTLEHCIRYSAPEEREFSMTFNFHHLKVDYKNGQKWELQPYDFAELK 120 Y+ +TVGEMSSTTLEHCI+Y+ PE +E SMTFNFHHLKVDY NG+KW + DF LK Sbjct: 245 SKYDAMTVGEMSSTTLEHCIQYTRPENKELSMTFNFHHLKVDYPNGEKWAIGDMDFFALK 304 Query: 121 ELFTRWQTGMQQAGGWNALFWNNHDQPRALSRFADDGKYRKESAKMLATTLHGMQGTPYV 180 E+ ++WQ GM + GGWNALFW NHDQPR +SR+ +DGKY ++SAKMLATT+H MQGTPY+ Sbjct: 305 EILSKWQVGMHKGGGWNALFWCNHDQPRVVSRYGNDGKYHEKSAKMLATTIHMMQGTPYI 364 Query: 181 YQGEELGMPNPDWHDISEFNDIESKNMYRILQERGLSGEQALDIIRERSRDNSRTPMQWD 240 YQGEE GM +P + I ++ D+E+ N Y IL+E+GLS E+ ++II+ +SRDNSRTP+QWD Sbjct: 365 YQGEEFGMTDPKFDRIDQYRDVETLNYYDILKEQGLSEEEVMEIIKRKSRDNSRTPVQWD 424 Query: 241 ASGHAGFTSGTPWLKVDRRYKEINVQAEQIDPDSIFAHYKQLIRLRKQEPVITDGIFKRL 300 S HAGFTSGTPW+ V Y+ IN + D SIF HYK+LI LRK ++T G ++ L Sbjct: 425 DSPHAGFTSGTPWIDVASNYRSINAKKALADKQSIFYHYKKLIELRKTYDIVTYGDYELL 484 Query: 301 DEGHPQIFAYARVGNTETLIVVSSFSGTQAGFSFADEFWSSRINSQAAELLIGNTEQTPV 360 P++FAY R ETL+VV+ F + ++ + + ++L+ N Sbjct: 485 LPEDPRVFAYVRRYQDETLLVVNHFFAGEVTIQVPEDVTAEK---HQVDVLLANYHDPSR 541 Query: 361 WSQSLEIPPYGSYMWIIR 378 Q L + PY S ++ ++ Sbjct: 542 EFQQLVLRPYESVVYYMK 559 >VC0911 [G] COG0366 Glycosidases Length = 562 Score = 385 bits (989), Expect = e-107, Method: Compositional matrix adjust. Identities = 189/329 (57%), Positives = 236/329 (71%), Gaps = 6/329 (1%) Query: 3 FWAEKGVDGFRMDVINLISKDQSFXXXXXXXXXXXXRRFYTDGPRVHEYIKELYAKVFGP 62 FWAEKGVDGFR+DVINLISK Q F RRFYTDGPRVHEY++E+ VF Sbjct: 192 FWAEKGVDGFRLDVINLISKQQDFTNDDIGDG----RRFYTDGPRVHEYLQEISRDVFQR 247 Query: 63 YNLVTVGEMSSTTLEHCIRYSAPEEREFSMTFNFHHLKVDYKNGQKWELQPYDFAELKEL 122 Y VTVGEMSSTTLEHC +YSA + RE SM FNFHHLKVDY NG+KW P+DF +LK++ Sbjct: 248 YGSVTVGEMSSTTLEHCQQYSALDGRELSMVFNFHHLKVDYPNGEKWTKAPFDFLQLKQI 307 Query: 123 FTRWQTGMQQAGGWNALFWNNHDQPRALSRFADDGKYRKESAKMLATTLHGMQGTPYVYQ 182 F WQTG+ GW ALFW NHDQPR +SR DD +YR ESAKMLA T+H MQGTPY+YQ Sbjct: 308 FNHWQTGL-NGKGWGALFWCNHDQPRIVSRLGDDQQYRVESAKMLAATIHLMQGTPYIYQ 366 Query: 183 GEELGMPNPDWHDISEFNDIESKNMYRIL-QERGLSGEQALDIIRERSRDNSRTPMQWDA 241 GEE+GM NP + I ++ D+ES N+++I+ Q++G+S L I+ ++SRDNSRTPMQWDA Sbjct: 367 GEEIGMTNPGFTSIKQYRDVESINIHQIMVQQQGVSESDMLAILAQKSRDNSRTPMQWDA 426 Query: 242 SGHAGFTSGTPWLKVDRRYKEINVQAEQIDPDSIFAHYKQLIRLRKQEPVITDGIFKRLD 301 S +AGFT G PW++V Y EIN QA DP S+F Y++L+ LRK+ VITDG + L Sbjct: 427 SLNAGFTRGEPWIEVAHNYPEINAQAALEDPHSVFYFYRRLLSLRKKVAVITDGDYTDLL 486 Query: 302 EGHPQIFAYARVGNTETLIVVSSFSGTQA 330 H +IFAY R + LI ++++ G +A Sbjct: 487 PEHKEIFAYQRRNQKQVLICLNNYYGHEA 515 >STM4453 [G] COG0366 Glycosidases Length = 550 Score = 356 bits (913), Expect = 4e-98, Method: Compositional matrix adjust. Identities = 181/378 (47%), Positives = 240/378 (63%), Gaps = 16/378 (4%) Query: 3 FWAEKGVDGFRMDVINLISKDQSFXXXXXXXXXXXXRRFYTDGPRVHEYIKELYAKVFGP 62 FWA++GVDG R+DV+NLI+KDQ+F RRFYTDGPR H +++E+ VF P Sbjct: 187 FWADRGVDGLRLDVVNLIAKDQNFPDDPTGDG----RRFYTDGPRAHTFLREMNRDVFTP 242 Query: 63 YNLVTVGEMSSTTLEHCIRYSAPEEREFSMTFNFHHLKVDYKNGQKWELQPYDFAELKEL 122 NL+TVGEMSSTTLE+C +Y+A E SMTFNFHHLKVDY NG+KW L D+ LK L Sbjct: 243 RNLMTVGEMSSTTLENCQQYAALSGDELSMTFNFHHLKVDYPNGEKWTLAKPDYVALKAL 302 Query: 123 FTRWQTGMQQAGGWNALFWNNHDQPRALSRFADDGKYRKESAKMLATTLHGMQGTPYVYQ 182 F WQ GM WNALFW NHDQPR +SRF D+G+YR +AKMLA LHGMQGTPY+YQ Sbjct: 303 FRHWQQGMHNVA-WNALFWCNHDQPRIVSRFGDEGEYRVPAAKMLAMALHGMQGTPYIYQ 361 Query: 183 GEELGMPNPDWHDISEFNDIESKNMYRILQERGLSGEQALDIIRERSRDNSRTPMQWDAS 242 GEE+GM NP + I+++ D+ES NM+ L+ G ++ L I+ +SRDNSRTPMQWD Sbjct: 362 GEEIGMTNPHFTRITDYRDVESHNMFAALRAAGRDPDELLAILASKSRDNSRTPMQWDNG 421 Query: 243 GHAGFTSGTPWLKVDRRYKEINVQAEQIDPDSIFAHYKQLIRLRKQEPVITDGIFKRLDE 302 +AGFT G PW+ + Y E+NV+A D +S+F Y++LI LRK +PV+ G ++ L Sbjct: 422 KNAGFTQGEPWISLCDNYTEVNVEAALRDENSVFYTYQKLIALRKTQPVLIWGDYQDLLP 481 Query: 303 GHPQIFAYARVGNTETLIVVSSFSGTQAGFSFADEFWSSRINSQAAELL--IGNTEQTPV 360 P ++ Y R + L+VV++ S E+ I Q LL G P Sbjct: 482 DSPSVWCYRRQWQGQILLVVANLSNQ------CQEWHPPHIKGQWQALLHNYGEVASQPA 535 Query: 361 WSQSLEIPPYGSYMWIIR 378 ++ + P+ + W+ R Sbjct: 536 ---AMTLRPFEAIWWLQR 550 >YPO3696 [G] COG0366 Glycosidases Length = 555 Score = 355 bits (912), Expect = 5e-98, Method: Compositional matrix adjust. Identities = 176/324 (54%), Positives = 220/324 (67%), Gaps = 5/324 (1%) Query: 3 FWAEKGVDGFRMDVINLISKDQSFXXXXXXXXXXXXRRFYTDGPRVHEYIKELYAKVFGP 62 FWA+ GVDG R+DVINL+SK Q F RRFYTDGPR+HE+++EL VF P Sbjct: 190 FWADLGVDGLRLDVINLVSKQQDFPNDLDGDG----RRFYTDGPRIHEFLQELSRDVFQP 245 Query: 63 YNLVTVGEMSSTTLEHCIRYSAPEEREFSMTFNFHHLKVDYKNGQKWELQPYDFAELKEL 122 L+TVGEMSST LEHC RY+A E SMTFNFHHLKVDY NG+KW L P + ELK + Sbjct: 246 RRLMTVGEMSSTRLEHCQRYAALGGDELSMTFNFHHLKVDYLNGEKWSLMPPNRVELKHI 305 Query: 123 FTRWQTGMQQAGGWNALFWNNHDQPRALSRFADDGKYRKESAKMLATTLHGMQGTPYVYQ 182 F +WQ GM WNALFW NHDQPR +SRF +G R +AKMLA LHGMQGTPY+YQ Sbjct: 306 FNQWQQGMHNRA-WNALFWCNHDQPRIVSRFGHEGALRLPAAKMLAMVLHGMQGTPYIYQ 364 Query: 183 GEELGMPNPDWHDISEFNDIESKNMYRILQERGLSGEQALDIIRERSRDNSRTPMQWDAS 242 GEE+GM NP++ DI+++ D+ES NM+ L G ++ L I+ +SRDNSRTPMQWD+S Sbjct: 365 GEEIGMTNPNFTDITQYRDVESLNMFAELSVAGREPDELLAILAAKSRDNSRTPMQWDSS 424 Query: 243 GHAGFTSGTPWLKVDRRYKEINVQAEQIDPDSIFAHYKQLIRLRKQEPVITDGIFKRLDE 302 +AGF+ GTPW+ Y EINV A D DS+F Y+ LI LRKQ + T G ++ L Sbjct: 425 DNAGFSQGTPWIAPCSNYHEINVNAALADADSVFYAYQYLIALRKQYDIFTFGDYQDLCP 484 Query: 303 GHPQIFAYARVGNTETLIVVSSFS 326 HP ++ Y R + L+VV++ S Sbjct: 485 QHPDLWCYLRSWQGKQLLVVANLS 508 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.135 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 29,359,806 Number of extensions: 1212752 Number of successful extensions: 4744 Number of sequences better than 1.0e-05: 53 Number of HSP's gapped: 4588 Number of HSP's successfully gapped: 53 Length of query: 378 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 270 Effective length of database: 25,922,359 Effective search space: 6999036930 Effective search space used: 6999036930 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)