BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0543.0033.p (288 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM1728 [R] COG3729 General stress protein 249 3e-66 PA4729 [H] COG0413 Ketopantoate hydroxymethyltransferase 221 8e-58 SMc01881 [H] COG0413 Ketopantoate hydroxymethyltransferase 199 3e-51 PA1598 [H] COG0413 Ketopantoate hydroxymethyltransferase 193 2e-49 mll0197 [H] COG0413 Ketopantoate hydroxymethyltransferase 191 1e-48 >STM1728 [R] COG3729 General stress protein Length = 60 Score = 249 bits (637), Expect = 3e-66, Method: Compositional matrix adjust. Identities = 0/246 (0%), Positives = 0/246 (0%), Gaps = 2/246 (0%) Query: 24 MLTAYDYPSALLAEEAGIDLILVGDSLGNVVLGYDTTLPVTIEDMVYHTRSVTRGARNTF 83 Sbjct: 17 76 Query: 84 IVADMPFMTYHGGIDETLRSVRRLMQEGRAHAVKMEGGLEICSTVSAVVSAGVPVLGHIG 143 Sbjct: 77 - 135 Query: 144 LTPQSVNMIGGYRIQGKDAKDAQRLMDEAKALEAAGVFGIXXXXXXXXXXXXISKAVSIP 203 Sbjct: 136 195 Query: 204 TIGIGAGRFCDGQVLVFHDLLRYASPYREKRFVKTYADVGTLIREGISSYVQEVKDRSFP 263 Sbjct: 196 - 254 Query: 264 AESHVF 269 Sbjct: 255 260 >PA4729 [H] COG0413 Ketopantoate hydroxymethyltransferase Length = 266 Score = 221 bits (564), Expect = 8e-58, Method: Compositional matrix adjust. Identities = 116/265 (43%), Positives = 164/265 (61%), Gaps = 2/265 (0%) Query: 7 LNIVKMKKMKADGVPLSMLTAYDYPSALLAEEAGIDLILVGDSLGNVVLGYDTTLPVTIE 66 + + ++ +K G ++MLT YD A A +AG+D++LVGDSLG V+ G+D+TLPV+ E Sbjct: 4 VTLTTLQGLKQSGEKIAMLTCYDATFAHTASQAGVDVLLVGDSLGMVLQGHDSTLPVSNE 63 Query: 67 DMVYHTRSVTRGARNTFIVADMPFMTYHGGIDETLRSVRRLMQEGRAHAVKMEGGLEICS 126 +M YHT V RG + + IV D+ F + H + +TL RLMQ G AH VK+EGG+ + Sbjct: 64 EMAYHTACVKRGNKGSLIVTDLAFESSHS-VAQTLADAVRLMQAG-AHMVKLEGGVWLAE 121 Query: 127 TVSAVVSAGVPVLGHIGLTPQSVNMIGGYRIQGKDAKDAQRLMDEAKALEAAGVFGIXXX 186 ++ + GVPV H+GLTPQ+VN+ GG+++QG+ A++L +A ALE AG + Sbjct: 122 PIARLAQMGVPVCAHLGLTPQAVNLFGGFKVQGRQETQARQLRADAIALEQAGAAMLLLE 181 Query: 187 XXXXXXXXXISKAVSIPTIGIGAGRFCDGQVLVFHDLLRYASPYREKRFVKTYADVGTLI 246 I++AV IP IGIGAG DGQVLV HD+L + R +FVK + I Sbjct: 182 CVPSVLAEEITQAVKIPVIGIGAGAATDGQVLVMHDMLGLSLTGRSPKFVKDFMQGQESI 241 Query: 247 REGISSYVQEVKDRSFPAESHVFNA 271 I++YV+ VKD SFPA H FNA Sbjct: 242 PAAIAAYVRAVKDVSFPAAEHGFNA 266 >SMc01881 [H] COG0413 Ketopantoate hydroxymethyltransferase Length = 273 Score = 199 bits (507), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 108/267 (40%), Positives = 158/267 (59%), Gaps = 3/267 (1%) Query: 4 KQALNIVKMKKMKADGVPLSMLTAYDYPSALLAEEAGIDLILVGDSLGNVVLGYDTTLPV 63 K+ L+ ++ +K + P+ LTAY P A L + +D +LVGDSLG V+ G DTT+ V Sbjct: 7 KRRLSPASIEALKGER-PIVSLTAYTTPIARLLDPH-VDFLLVGDSLGMVLYGLDTTVGV 64 Query: 64 TIEDMVYHTRSVTRGARNTFIVADMPFMTYHGGIDETLRSVRRLMQEGRAHAVKMEGGLE 123 T++ M+ H ++V RG+ +V D+PF Y ++ RS R+++E AVK+EGG E Sbjct: 65 TLDMMIAHGQAVMRGSERCCVVVDLPFGAYQESKEQAFRSAARILKETGCSAVKLEGGAE 124 Query: 124 ICSTVSAVVSAGVPVLGHIGLTPQSVNMIGGYRIQGKDAKDAQRLMDEAKALEAAGVFGI 183 + TV +VS G+PVLGH+GL PQ VN GGYR G++ K+ ++ +AKA++ AG F I Sbjct: 125 MAETVEFLVSRGIPVLGHVGLMPQLVNTTGGYRSVGRNEKEVAKIRRDAKAIDDAGAFAI 184 Query: 184 XXXXXXXXXXXXISKAVSIPTIGIGAGRFCDGQVLVFHDLLRYASPYREKRFVKTYADVG 243 I+ + PTIGIGA CDGQ+LV D+L + ++ RFVK +A++ Sbjct: 185 VVEGTVEPVAREITATLRAPTIGIGASPACDGQILVSDDMLGLFNDFK-PRFVKHFAELA 243 Query: 244 TLIREGISSYVQEVKDRSFPAESHVFN 270 I + +Y EVK RSFP H F Sbjct: 244 PTISKAAEAYANEVKARSFPGIEHTFQ 270 >PA1598 [H] COG0413 Ketopantoate hydroxymethyltransferase Length = 273 Score = 193 bits (491), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 109/270 (40%), Positives = 157/270 (58%), Gaps = 3/270 (1%) Query: 4 KQALNIVKMKKMKADGVPLSMLTAYDYPSALLAEEAGIDLILVGDSLGNVVLGYDTTLPV 63 + L++ +++ K G L LTAY P A L + DL+LVGDSLG V+ G +TL V Sbjct: 7 QHRLSVPDIQRRKGAGS-LVALTAYSTPMARLLDPHA-DLLLVGDSLGMVLYGMPSTLGV 64 Query: 64 TIEDMVYHTRSVTRGARNTFIVADMPFMTYHGGIDETLRSVRRLMQEGRAHAVKMEGGLE 123 ++E MV HT +V RG+R +VAD+PF +Y + R+ RL+ + A AVK+EGG E Sbjct: 65 SLEMMVAHTLAVMRGSRRACVVADLPFASYQESPRQAFRNAARLLADSGAQAVKLEGGEE 124 Query: 124 ICSTVSAVVSAGVPVLGHIGLTPQSVNMIGGYRIQGKDAKDAQRLMDEAKALEAAGVFGI 183 + TV +V G+PVL HIGL PQ VN +GG++ QG+D + A+R+ + A++ G F + Sbjct: 125 MEETVDFLVRRGIPVLAHIGLMPQQVNAMGGFKAQGRDPESAERVRRDGLAMQRGGAFAV 184 Query: 184 XXXXXXXXXXXXISKAVSIPTIGIGAGRFCDGQVLVFHDLLRYASPYREKRFVKTYADVG 243 +S+ ++IP IGIGA CDGQVLV DLL S + RFV+ YA + Sbjct: 185 VIEGVGEPLARRLSEELAIPCIGIGASPACDGQVLVSEDLLGL-SGEQVPRFVERYARLD 243 Query: 244 TLIREGISSYVQEVKDRSFPAESHVFNADE 273 I E + ++V++R FP H F E Sbjct: 244 REIDEAARRFAEDVRERRFPEARHCFAMRE 273 >mll0197 [H] COG0413 Ketopantoate hydroxymethyltransferase Length = 301 Score = 191 bits (485), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 21/260 (8%), Positives = 56/260 (21%), Gaps = 2/260 (0%) Query: 11 KMKKMKADGVPLSMLTAYDYPSALLAEEAGIDLILVGDSLGNVVLGYDTTLPVTIEDMVY 70 + + +L ++ A+ I+ + G Y T + +E+ Sbjct: 15 GRPNVGKSTLLNKLLGQKISITSRKAQTT-RHRIVGIHTEGAYQAIYVDTPGLHMEEKRA 73 Query: 71 HTRSVTRGARNTFIVADMPFMTYHGGIDETLRSVRRLMQEGRAHAVKMEGGLEICSTVSA 130 R + + A ++ ++ G + V + A Sbjct: 74 INRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREGKAPVILAVNKVDNVQEKA 133 Query: 131 VVSAGVPVLGHIGLTPQSVNMIGGYRIQGKDAKDAQRLMDEAKALEAAGVFGIXXXXXXX 190 + + L V + + R + Sbjct: 134 DLLPHLQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHLPEATHHFPEDYITDRSQRFM 193 Query: 191 XXXXXISKAVSIPTIGIGAGRFCDGQVLVFHDLLRYASPYREKRFVKTYADVGTLIREGI 250 K + + + + V ++ Y + + +G Sbjct: 194 ASEIIREKLMRFLGAELPYSVTVEIERFVSNERGGY-DINGLILVEREGQKKMVIGNKGA 252 Query: 251 SSYVQEVKDRSFPAESHVFN 270 ++ R E Sbjct: 253 KIKTIGIEARKDMQEMFEAP 272 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,925,506 Number of extensions: 834303 Number of successful extensions: 1698 Number of sequences better than 1.0e-05: 8 Number of HSP's gapped: 1690 Number of HSP's successfully gapped: 8 Length of query: 288 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 183 Effective length of database: 26,268,361 Effective search space: 4807110063 Effective search space used: 4807110063 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)