BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0543.0027.p (433 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1692 [FR] COG0402 Cytosine deaminase and related metal-depende... 391 e-108 MA1276 [FR] COG0402 Cytosine deaminase and related metal-depende... 355 1e-97 MJ1541 [FR] COG0402 Cytosine deaminase and related metal-depende... 345 6e-95 MTH1505 [FR] COG0402 Cytosine deaminase and related metal-depend... 323 4e-88 MK0366 [FR] COG0402 Cytosine deaminase and related metal-depende... 321 2e-87 >BH1692 [FR] COG0402 Cytosine deaminase and related metal-dependent hydrolases Length = 428 Score = 391 bits (1005), Expect = e-108, Method: Compositional matrix adjust. Identities = 199/414 (48%), Positives = 272/414 (65%), Gaps = 5/414 (1%) Query: 25 GYMTIEKDLITYIGEVKPVIEE--GTQTIDGSRLLFMPGLVNTHGHTAMSLLRGYGDDMV 82 GY +I + E P EE + IDG MPG++NTHGH MSLLRG+ DD+ Sbjct: 13 GYFKFADGVIVSVAEGTPSPEEIDRVEVIDGKGKWVMPGMINTHGHLGMSLLRGHSDDLP 72 Query: 83 LQNWLQEKMWPMEEKFTGDDVYWGTALSVLEMLKGGTTTFLDMYD-HMDRVAEVTEQSGI 141 LQ+WL EKMWP E K + V+ L++ EM+K GTTTFL+MY +MD +AE + G Sbjct: 73 LQSWLTEKMWPFEGKMDREAVHHARQLAMAEMIKSGTTTFLEMYHLYMDDLAEAVVEQGP 132 Query: 142 RAVLMRGVIGLCPEDVQNHKLAEAVAFARNWHGKADGRITTMISPHAPYTCPPEFFVKFV 201 RAVLMR +IGLC E Q KL EAV FA W+G +GRITTM++PHAPYTCPP F V Sbjct: 133 RAVLMRSMIGLCSESEQREKLKEAVTFATTWNGDGNGRITTMMAPHAPYTCPPSFIEMIV 192 Query: 202 QAAHDLDLPMHTHMSETRREVEQNAADYGLRPVAHLEKLGMFT-RPSLVAHGVHLNDEEI 260 A +DLP+HTHM+ET+REVE++ YG+ P+ H E+LG R L+AH VHL +EE+ Sbjct: 193 DEADRIDLPLHTHMAETQREVEEHRKTYGVHPLVHFEQLGFLKDRHWLLAHCVHLGEEEL 252 Query: 261 EILARY-HVGVSHNPGSNLKLASGVARVPELLRAGVTVSLGTDGAASNNNLDMFEEMRLA 319 +IL ++ V VSHNP SNLKL SG+A V +L G+ + LGTD ASNN+LD+ EEMR+A Sbjct: 253 DILEQHPSVHVSHNPMSNLKLGSGIANVQSMLERGINICLGTDSVASNNHLDLVEEMRIA 312 Query: 320 ALIHKGVSGDPTAVPAQEALLMATEYGAQSIFLEGVGRLAPGMKADFIALDIDQPHFLPH 379 AL+ KG DPTA+PA+ A+ MAT+ GA+++ L VG + G +ADFI +D H PH Sbjct: 313 ALLQKGAVLDPTAIPAETAIAMATKNGAKALRLPQVGTIEAGKRADFIMIDPQCLHLQPH 372 Query: 380 TDLLSHAVYSASAKDVAHVWVDGKQVVKHGQCLTLDEEQIRRKAQETFEGLLKR 433 ++SH VY+ DV V+V+G ++ + + T DEE+++ +A ++ + ++ Sbjct: 373 EHVMSHLVYALKGADVQDVFVEGAPLMLNKELKTFDEEKLQFEANAHYQRICEK 426 >MA1276 [FR] COG0402 Cytosine deaminase and related metal-dependent hydrolases Length = 442 Score = 355 bits (910), Expect = 1e-97, Method: Compositional matrix adjust. Identities = 192/410 (46%), Positives = 250/410 (60%), Gaps = 3/410 (0%) Query: 24 SGYMTIEKDLITYIGEVKPVIEEGTQTIDGSRLLFMPGLVNTHGHTAMSLLRGYGDDMVL 83 +G + IE IT IGE E ID + MPGL NTH H AM+L RGY DD+ L Sbjct: 33 NGTVVIEDGKITEIGE--NTKENADTVIDAKGSVVMPGLANTHTHAAMTLFRGYADDLQL 90 Query: 84 QNWLQEKMWPMEEKFTGDDVYWGTALSVLEMLKGGTTTFLDMYDHMDRVAEVTEQSGIRA 143 WL++ +WP E + +DVY G+ L+ LEM+K GTT+F DMY +MD A+ E SG+RA Sbjct: 91 AEWLEKHIWPAEAQLKAEDVYKGSLLACLEMIKSGTTSFADMYFYMDETAKAVEASGLRA 150 Query: 144 VLMRGVIGLCPEDVQNHKLAEAVAFARNWHGKADGRITTMISPHAPYTCPPEFFVKFVQA 203 L G+I L E+ L E F R W G ADGRI TM PHAP TC EF K + Sbjct: 151 SLSHGLIELWNEEKGEADLKEGKRFVRAWQGAADGRIKTMYGPHAPNTCSEEFLTKVKEE 210 Query: 204 AHDLDLPMHTHMSETRREVEQNAADYGLRPVAHLEKLGMFTRPSLVAHGVHLNDEEIEIL 263 AH +H H+ ET E+ YG V LE +G F L AH V L+D +IEIL Sbjct: 211 AHRDGAGLHIHVLETEAELNAMKERYGKCSVHLLEDIGFFGPDVLAAHCVWLSDGDIEIL 270 Query: 264 ARYHVGVSHNPGSNLKLASGVARVPELLRAGVTVSLGTDGAASNNNLDMFEEMRLAALIH 323 + V VSHNP SN+KLASG+A V ++L GV V+LGTDG ASNNNLD+FEE++ AAL+H Sbjct: 271 RQREVNVSHNPISNMKLASGIAPVYKMLEKGVNVTLGTDGCASNNNLDLFEEIKTAALLH 330 Query: 324 KGVSGDPTAVPAQEALLMATEYGAQSIFLEGVGRLAPGMKADFIALDIDQPHFLPHTDLL 383 K +G+PTA+PA++ L MAT GA+++ E G L G KAD I +D+ +PH P D+ Sbjct: 331 KVSTGNPTALPARQVLEMATVNGAKALGTE-TGMLKVGKKADMIVVDMKKPHLTPCFDVP 389 Query: 384 SHAVYSASAKDVAHVWVDGKQVVKHGQCLTLDEEQIRRKAQETFEGLLKR 433 SH VYSA DV VDGK ++ + + L +DEE++ +A+ E L+ R Sbjct: 390 SHLVYSAKGCDVRTTIVDGKVLMDNYRVLVMDEEKVIEEARTAAEELVAR 439 >MJ1541 [FR] COG0402 Cytosine deaminase and related metal-dependent hydrolases Length = 420 Score = 345 bits (886), Expect = 6e-95, Method: Compositional matrix adjust. Identities = 176/427 (41%), Positives = 265/427 (62%), Gaps = 14/427 (3%) Query: 5 KTVIVNGRFLVPGTAQPVLSGYMTIEKDLITYIGEVKPVIEEGTQTIDGSRLLFMPGLVN 64 K V VNG+ Q +L IE + I IGEVK E + IDG + +PGL+N Sbjct: 5 KNVFVNGK------RQDIL-----IEGNKIKKIGEVKKEEIENAEIIDGKNKIAIPGLIN 53 Query: 65 THGHTAMSLLRGYGDDMVLQNWLQEKMWPMEEKFTGDDVYWGTALSVLEMLKGGTTTFLD 124 TH H M+L RG DD+ L WL +WPME K + VYWGT L +EM++ GTTTF D Sbjct: 54 THTHIPMTLFRGVADDLPLMEWLNNYIWPMEAKLNEEIVYWGTLLGCIEMIRSGTTTFND 113 Query: 125 MYDHMDRVAEVTEQSGIRAVLMRGVIGLCPEDVQNHKLAEAVAFARNWHGKADGRITTMI 184 MY ++ +A+ ++SG+RAVL G+I L E+ + +L A + + + RI + Sbjct: 114 MYFFLEGIAKAVDESGMRAVLAYGMIDLFDEERRERELKNAEKYINYINSLNNSRIMPAL 173 Query: 185 SPHAPYTCPPEFFVKFVQAAHDLDLPMHTHMSETRREVEQNAADYGLRPVAHLEKLGMFT 244 PHAPYTC E ++ A ++P+H H++ET E++ G+ P +L G F Sbjct: 174 GPHAPYTCSKELLMEVNNLAKKYNVPIHIHLNETLDEIKMVKEKTGMEPFIYLNSFGFFD 233 Query: 245 R-PSLVAHGVHLNDEEIEILARYHVGVSHNPGSNLKLASGVARVPELLRAGVTVSLGTDG 303 ++ AH VHL DEEI+I+ + ++ VSHNP SNLKLASGVA +P+LL G+ V+LGTDG Sbjct: 234 DVRAIAAHCVHLTDEEIKIMKQKNINVSHNPISNLKLASGVAPIPKLLAEGINVTLGTDG 293 Query: 304 AASNNNLDMFEEMRLAALIHKGVSGDPTAVPAQEALLMATEYGAQSIFLEGVGRLAPGMK 363 SNNNL++FEE++++A++HKGV+ +PT V A+EA AT+ GA+++ ++ G + G Sbjct: 294 CGSNNNLNLFEEIKVSAILHKGVNLNPTVVKAEEAFNFATKNGAKALNIKA-GEIREGYL 352 Query: 364 ADFIALDIDQPHFLPHTDLLSHAVYSASAKDVAHVWVDGKQVVKHGQCLTLDEEQIRRKA 423 AD + +++D+P+ P +++SH VY+ + V V +DG V++ G+ LT+DEE++ KA Sbjct: 353 ADIVLINLDKPYLYPKENIMSHLVYAFNGF-VDDVIIDGNIVMRDGEILTVDEEKVYEKA 411 Query: 424 QETFEGL 430 +E +E L Sbjct: 412 EEMYEIL 418 >MTH1505 [FR] COG0402 Cytosine deaminase and related metal-dependent hydrolases Length = 268 Score = 323 bits (828), Expect = 4e-88, Method: Compositional matrix adjust. Identities = 0/418 (0%), Positives = 0/418 (0%), Gaps = 8/418 (1%) Query: 4 NKTVIVNGRFLVPGTAQPVLSGYMTIEKDLITYIGEVKPVIEEGT--QTIDGSRLLFMPG 61 + + + TA+ T + I + D + Sbjct: 3 RVDLSIFIPDSL--TAETGDLKIKTYKVV---LIARAASIFGVKRIVIYHDDADGEARFI 57 Query: 62 LVNTHGHTAMSLLRGYGDDMVLQNWLQEKMWPMEEKFTGDDVYWGTALSVLEMLKGGTTT 121 LR ++ + + P+ +K Sbjct: 58 RDILTYMDTPQYLRRKVFPIMRELKHVGILPPLRTPHHPTGKPVTGEYRQGLTVKRVKKG 117 Query: 122 FLDMYDHMDRVAEVTEQSGIRAVLMRGVIGLCPEDVQNHKLAEAVAFARNWHGKADGRIT 181 L + + R + R V ++ + + + + + Sbjct: 118 TLVDIGADKLALCREKLTVNRIMSFRVVRLGKEILIEPDEPEDRYWGYEVLDTRRNLAES 177 Query: 182 TMISPHAPYTCPPEFFVKFVQAAHDLDLPMHTHMSETRREVEQNAADYGLRPVAHLEKLG 241 ++ M + + L Sbjct: 178 LKTVGADVVVATSRNASPITSILDEVKTRMRGAREAAILFGGPYKGLPEIDADIWVNTLP 237 Query: 242 MFTRPSLVAHGVHLNDEEIEILARYHVGVSHNPGSNLKLASGVARVPELLRAGVTVSLGT 301 Sbjct: 238 297 Query: 302 DGAASNNNLDMFEEMRLAALIHKGVSGDPTAVPAQEALLMATEYGAQSIFLEGVGRLAPG 361 Sbjct: 298 - 356 Query: 362 MKADFIALDIDQPHFLPHTDLLSHAVYSASAKDVAHVWVDGKQVVKHGQCLTLDEEQI 419 Sbjct: 357 414 >MK0366 [FR] COG0402 Cytosine deaminase and related metal-dependent hydrolases Length = 431 Score = 321 bits (822), Expect = 2e-87, Method: Compositional matrix adjust. Identities = 166/378 (43%), Positives = 232/378 (61%), Gaps = 1/378 (0%) Query: 53 GSRLLFMPGLVNTHGHTAMSLLRGYGDDMVLQNWLQEKMWPMEEKFTGDDVYWGTALSVL 112 G + + MPGL+NTH H M+L RG DDM L WL+E++WP+EE+ + WG AL+ + Sbjct: 52 GEKDVIMPGLINTHTHGPMTLFRGVADDMPLMKWLREEIWPLEERLDAEKCRWGAALAAM 111 Query: 113 EMLKGGTTTFLDMYDHMDRVAEVTEQSGIRAVLMRGVIGLCPEDVQNHKLAEAVAFARNW 172 E LK GTT DMY MD VAE + GIRAV+ G+I L ED + +L E+ R Sbjct: 112 EALKSGTTCLADMYFFMDAVAEAYAEVGIRAVISHGMIDLGEEDKREEELKESKRVYRKC 171 Query: 173 HGKADGRITTMISPHAPYTCPPEFFVKFVQAAHDLDLPMHTHMSETRREVEQNAADYGLR 232 G +G I + PHAPYTC E + + A + + + H++ET EV++ +G R Sbjct: 172 QG-MEGLIEFSLGPHAPYTCSEELLKEVRRLADEWGVKIQIHVAETEDEVKEVKRKHGKR 230 Query: 233 PVAHLEKLGMFTRPSLVAHGVHLNDEEIEILARYHVGVSHNPGSNLKLASGVARVPELLR 292 PV +L+++G+ + AH V L+D+EIEIL++ V VSHNP SN+KLASG++ VPE+L Sbjct: 231 PVEYLDEIGLLGDDVIAAHCVWLDDKEIEILSKRGVIVSHNPISNMKLASGISPVPEMLE 290 Query: 293 AGVTVSLGTDGAASNNNLDMFEEMRLAALIHKGVSGDPTAVPAQEALLMATEYGAQSIFL 352 GV V++GTDG ASNNNLDM EE+++AAL+HK DP+A E L MAT Sbjct: 291 RGVNVTIGTDGCASNNNLDMLEEIKVAALLHKVNKMDPSATEMLEILRMATVRAGTVFSS 350 Query: 353 EGVGRLAPGMKADFIALDIDQPHFLPHTDLLSHAVYSASAKDVAHVWVDGKQVVKHGQCL 412 E +G + G AD + LD P P+ + +S+ VYSAS DV HV+V G+ VVK+G+ + Sbjct: 351 EKIGAIEEGYAADLVVLDGSSPRLNPNHNPISNIVYSASGSDVKHVFVAGELVVKNGKLV 410 Query: 413 TLDEEQIRRKAQETFEGL 430 DE++I + E E L Sbjct: 411 KADEQEILENSTECAEQL 428 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.135 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 33,636,765 Number of extensions: 1387441 Number of successful extensions: 3203 Number of sequences better than 1.0e-05: 53 Number of HSP's gapped: 3117 Number of HSP's successfully gapped: 53 Length of query: 433 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 324 Effective length of database: 25,807,025 Effective search space: 8361476100 Effective search space used: 8361476100 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)