BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0543.0023.p (500 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value slr0228 [O] COG0465 ATP-dependent Zn proteases 330 3e-90 slr1604 [O] COG0465 ATP-dependent Zn proteases 330 3e-90 alr1261 [O] COG0465 ATP-dependent Zn proteases 325 1e-88 all3642 [O] COG0465 ATP-dependent Zn proteases 320 3e-87 BS_ftsH [O] COG0465 ATP-dependent Zn proteases 318 1e-86 >slr0228 [O] COG0465 ATP-dependent Zn proteases Length = 627 Score = 330 bits (847), Expect = 3e-90, Method: Compositional matrix adjust. Identities = 207/501 (41%), Positives = 295/501 (58%), Gaps = 37/501 (7%) Query: 18 IFMAFVGINIFPVIIAAGMVGALLLIAHLRGG--LTVNAGGDKKR-KKNGPSKLTFEEIG 74 + FVG IFPV++ A + +++ GG +N G K R + + + + F+++ Sbjct: 116 MVWGFVGNLIFPVLLIASLFFLFRRSSNMPGGPGQAMNFGKSKARFQMDAKTGVMFDDVA 175 Query: 75 GQDNAKQELREALDFLIRHEEISKFGIRPLKGILLTGPPGTGKTLMAKAAAHYTNSVFVA 134 G D AK+EL+E + FL + E + G + KG+LL GPPGTGKTL+AKA A F + Sbjct: 176 GIDEAKEELQEVVTFLKQPERFTAVGAKIPKGVLLVGPPGTGKTLLAKAIAGEAGVPFFS 235 Query: 135 ASGSEFVEMYVGVGAGRVRDLFKDARARALKENKQSAIIFIDEIDVIGGKRE---GGQQR 191 SGSEFVEM+VGVGA RVRDLFK A KEN +IFIDEID +G +R GG Sbjct: 236 ISGSEFVEMFVGVGASRVRDLFKKA-----KENA-PCLIFIDEIDAVGRQRGAGIGGGND 289 Query: 192 EYDQTLNQLLTEMDGIYNNDTPRILLVAATNRKEMLDSALLRPGRFDRHIQVDMPDKKGR 251 E +QTLNQLLTEMDG N I+++AATNR ++LDSAL+RPGRFDR + VD PD GR Sbjct: 290 EREQTLNQLLTEMDGFEGNTG--IIIIAATNRPDVLDSALMRPGRFDRQVMVDAPDYSGR 347 Query: 252 RSILDLHAQNKPLHEEVDLDKIAEEAYGFSGAQLESVMNEAAIYMMRENLTQVEQRHLSL 311 + IL++HA+NK L EV +D IA GFSGA L +++NEAAI R + + + Sbjct: 348 KEILEVHARNKKLAPEVSIDSIARRTPGFSGADLANLLNEAAILTARRRKSAITLLEIDD 407 Query: 312 AIDKVMMGEKTDRETNHEEKKRVAIHELGHAIMAELLRP-GSVSQVTLTPRGQALGYVRH 370 A+D+V+ G + + + K+ +A HE+GHAI+ LL+ V +VTL PRGQA G Sbjct: 408 AVDRVVAGMEGTPLVDSKSKRLIAYHEVGHAIVGTLLKDHDPVQKVTLIPRGQAQGLTWF 467 Query: 371 NPQTEQYLYTKDYLENQIMIALGGAVAEEMYYGGR--STGSRGDFDQALNIVETMM-KSG 427 P EQ L TK L +I A+GG AEE +G +TG+ GD Q + M+ + G Sbjct: 468 TPNEEQGLTTKAQLMARIAGAMGGRAAEEEVFGDDEVTTGAGGDLQQVTEMARQMVTRFG 527 Query: 428 LTSLGIANLQ-----------MVTTEELMKENSKILD----ELMVRTHELLGK----QRN 468 +++LG +L+ ++ E +E + +D +L + H++ K QR Sbjct: 528 MSNLGPISLESSGGEVFLGGGLMNRSEYSEEVATRIDAQVRQLAEQGHQMARKIVQEQRE 587 Query: 469 VFDYSLDILMKEEVLSGEQFR 489 V D +D+L+++E + GE+FR Sbjct: 588 VVDRLVDLLIEKETIDGEEFR 608 >slr1604 [O] COG0465 ATP-dependent Zn proteases Length = 616 Score = 330 bits (846), Expect = 3e-90, Method: Compositional matrix adjust. Identities = 199/496 (40%), Positives = 291/496 (58%), Gaps = 40/496 (8%) Query: 27 IFPVIIAAGMVGALLLIAHLRGGLTVNAGGDKKRKKNGP-SKLTFEEIGGQDNAKQELRE 85 P+++ G+ G +N G K R + P +++TF ++ G + AK EL E Sbjct: 116 FLPILLLVGIFFLFRRAQSGPGSQAMNFGKSKARVQMEPQTQVTFGDVAGIEQAKLELTE 175 Query: 86 ALDFLIRHEEISKFGIRPLKGILLTGPPGTGKTLMAKAAAHYTNSVFVAASGSEFVEMYV 145 +DFL + ++ G + KG+LL GPPGTGKTL+AKA A F + SGSEFVEM+V Sbjct: 176 VVDFLKNADRFTELGAKIPKGVLLVGPPGTGKTLLAKAVAGEAGVPFFSISGSEFVEMFV 235 Query: 146 GVGAGRVRDLFKDARARALKENKQSAIIFIDEIDVIGGKRE---GGQQREYDQTLNQLLT 202 GVGA RVRDLF+ A+A A I+FIDEID +G +R GG E +QTLNQLLT Sbjct: 236 GVGASRVRDLFEQAKANA------PCIVFIDEIDAVGRQRGAGLGGGNDEREQTLNQLLT 289 Query: 203 EMDGIYNNDTPRILLVAATNRKEMLDSALLRPGRFDRHIQVDMPDKKGRRSILDLHAQNK 262 EMDG N I++VAATNR ++LDSAL+RPGRFDR + VD PD GRR IL++HA+ K Sbjct: 290 EMDGFEGNTG--IIIVAATNRPDVLDSALMRPGRFDRQVVVDRPDYAGRREILNVHARGK 347 Query: 263 PLHEEVDLDKIAEEAYGFSGAQLESVMNEAAIYMMRENLTQVEQRHLSLAIDKVMMG-EK 321 L ++VDLDKIA GF+GA L +++NEAAI R NLT++ ++ AID+V+ G EK Sbjct: 348 TLSQDVDLDKIARRTPGFTGADLSNLLNEAAILAARRNLTEISMDEVNDAIDRVLAGPEK 407 Query: 322 TDRETNHEEKKRVAIHELGHAIMAELLRP-GSVSQVTLTPRGQALGYVRHNP---QTEQY 377 +R + + K VA HE GHA++ L+ V ++++ PRG+A G P + E Sbjct: 408 KNRVMSEKRKTLVAYHEAGHALVGALMPDYDPVQKISIIPRGRAGGLTWFTPSEDRMESG 467 Query: 378 LYTKDYLENQIMIALGGAVAEEMYYGGR--STGSRGDFDQALNIVETMM-------KSGL 428 LY++ YL+NQ+ +ALGG +AEE+ +G +TG+ D Q + M+ + G Sbjct: 468 LYSRSYLQNQMAVALGGRIAEEIIFGEEEVTTGASNDLQQVARVARQMVTRFGMSDRLGP 527 Query: 429 TSLG-----------IA---NLQMVTTEELMKENSKILDELMVRTHELLGKQRNVFDYSL 474 +LG IA + T + +E S+++D+ R ++L + R + D Sbjct: 528 VALGRQGGGVFLGRDIASDRDFSDETAAAIDEEVSQLVDQAYQRAKQVLVENRGILDQLA 587 Query: 475 DILMKEEVLSGEQFRC 490 +IL+++E + E+ + Sbjct: 588 EILVEKETVDSEELQT 603 >alr1261 [O] COG0465 ATP-dependent Zn proteases Length = 359 Score = 325 bits (833), Expect = 1e-88, Method: Compositional matrix adjust. Identities = 0/474 (0%), Positives = 0/474 (0%), Gaps = 40/474 (8%) Query: 48 GGLTVNAGGDKKRKKNGP-SKLTFEEIGGQDNAKQELREALDFLIRHEEISKFGIRPLKG 106 + KN D K E + K G R + Sbjct: 134 PEQAEAFYHRLTQCKNVRQPVNIVSHFARADEPKCGATEKQLAIFNTFCEGKPGQRSIAA 193 Query: 107 ILLTGPPGTGKTLMAKAAAHYTNSVFVAASGSEFVEMYVGVGAGRVRDLFKDARARALKE 166 + + + V + + Sbjct: 194 SGGILLWPQSHFDWVRPGIILYGVSPLEDRSTGADFGCQPVMSLTSSLIAVREHKAG--- 250 Query: 167 NKQSAIIFIDEIDVIGGKRE---GGQQREYDQTLNQLLTEMDGIYNNDTPRILLVAATNR 223 + + R + T + I+ A + Sbjct: 251 ---EPVGYGGTWVSERDTRLGVVAMGYGDGYPRAAPSGTPVLVNGREVP--IVGRVAMDM 305 Query: 224 KEMLDSALLRPGRFDRHIQVDMPDKKGRRSILDLHAQNKPLHEEVDLDKIAEEAYGFSGA 283 Sbjct: 306 365 Query: 284 QLESVMNEAAIYMMRENLTQVEQRHLSLAIDKVMMG-EKTDRETNHEEKKRVAIHELGHA 342 Sbjct: 366 425 Query: 343 IMAELLRP-GSVSQVTLTPRGQALGYVRHNPQTEQY---LYTKDYLENQIMIALGGAVAE 398 Sbjct: 426 485 Query: 399 EMYYGGR--STGSRGDFDQALNIVETMM-------KSGLTSLGIANLQMVTTEELMKENS 449 Sbjct: 486 545 Query: 450 --------------KILDELMVRTHELLGKQRNVFDYSLDILMKEEVLSGEQFR 489 Sbjct: 546 599 >all3642 [O] COG0465 ATP-dependent Zn proteases Length = 628 Score = 320 bits (820), Expect = 3e-87, Method: Compositional matrix adjust. Identities = 195/492 (39%), Positives = 281/492 (57%), Gaps = 37/492 (7%) Query: 27 IFPVIIAAGMVGALLLIAHLRGG--LTVNAGGDKKR-KKNGPSKLTFEEIGGQDNAKQEL 83 +FP+++ G+ +L GG +N G K R + + + F+++ G + AK+EL Sbjct: 126 VFPILLITGLFFLFRRSNNLPGGPGQAMNFGKSKARFQMEAKTGVKFDDVAGIEEAKEEL 185 Query: 84 REALDFLIRHEEISKFGIRPLKGILLTGPPGTGKTLMAKAAAHYTNSVFVAASGSEFVEM 143 +E + FL + E + G R KG+LL GPPGTGKTL+AKA A F + SGSEFVEM Sbjct: 186 QEVVTFLKQPERFTAVGARIPKGVLLVGPPGTGKTLLAKAIAGEAGVPFFSISGSEFVEM 245 Query: 144 YVGVGAGRVRDLFKDARARALKENKQSAIIFIDEIDVIGGKRE---GGQQREYDQTLNQL 200 +VGVGA RVRDLFK A+ A IIFIDEID +G +R GG E +QTLNQL Sbjct: 246 FVGVGASRVRDLFKKAKDNA------PCIIFIDEIDAVGRQRGAGIGGGNDEREQTLNQL 299 Query: 201 LTEMDGIYNNDTPRILLVAATNRKEMLDSALLRPGRFDRHIQVDMPDKKGRRSILDLHAQ 260 LTEMDG N I+++AATNR ++LD+ALLRPGRFDR + VD PD KGR +L +HA+ Sbjct: 300 LTEMDGFEGNTG--IIIIAATNRPDVLDAALLRPGRFDRQVTVDAPDIKGRLEVLQVHAR 357 Query: 261 NKPLHEEVDLDKIAEEAYGFSGAQLESVMNEAAIYMMRENLTQVEQRHLSLAIDKVMMGE 320 NK L V L+ IA GF+GA L +++NEAAI R + + A+D+V+ G Sbjct: 358 NKKLDPSVSLEAIARRTPGFTGADLANLLNEAAILTARRRKEGITLSEIDDAVDRVVAGM 417 Query: 321 KTDRETNHEEKKRVAIHELGHAIMAELLRP-GSVSQVTLTPRGQALGYVRHNPQTEQYLY 379 + + + K+ +A HE+GHA++ LL+ V +VTL PRGQA G P EQ L Sbjct: 418 EGTPLVDSKSKRLIAYHEVGHALVGTLLKDHDPVQKVTLIPRGQAQGLTWFTPNEEQGLI 477 Query: 380 TKDYLENQIMIALGGAVAEEMYYGGR--STGSRGDFDQALNIVETMM-KSGLTSLGIANL 436 ++ L+ +I ALGG AEE+ +G +TG+ GD Q + M+ + G++ LG +L Sbjct: 478 SRSQLKARITGALGGRAAEEIIFGSAEVTTGAGGDLQQVSGMARQMVTRFGMSDLGPLSL 537 Query: 437 QMVTTEELMKENSK-------------------ILDELMVRTHELLGKQRNVFDYSLDIL 477 + E + + I+DE +++ + R V D +D+L Sbjct: 538 ESQQGEVFLGRDWMTRSDYSESIAARIDSQVRLIVDECYQNAKKIMREHRTVTDRIVDLL 597 Query: 478 MKEEVLSGEQFR 489 +++E + GE+FR Sbjct: 598 IEKETIDGEEFR 609 >BS_ftsH [O] COG0465 ATP-dependent Zn proteases Length = 637 Score = 318 bits (816), Expect = 1e-86, Method: Compositional matrix adjust. Identities = 188/450 (41%), Positives = 265/450 (58%), Gaps = 35/450 (7%) Query: 67 KLTFEEIGGQDNAKQELREALDFLIRHEEISKFGIRPLKGILLTGPPGTGKTLMAKAAAH 126 ++ F+++ G D KQEL E ++FL + ++ G R KG+LL GPPGTGKTL+AKA A Sbjct: 157 RVKFKDVAGADEEKQELVEVVEFLKDPRKFAELGARIPKGVLLVGPPGTGKTLLAKACAG 216 Query: 127 YTNSVFVAASGSEFVEMYVGVGAGRVRDLFKDARARALKENKQSAIIFIDEIDVIGGKRE 186 F + SGS+FVEM+VGVGA RVRDLF++A+ A +IFIDEID +G +R Sbjct: 217 EAGVPFFSISGSDFVEMFVGVGASRVRDLFENAKKNA------PCLIFIDEIDAVGRQRG 270 Query: 187 ---GGQQREYDQTLNQLLTEMDGIYNNDTPRILLVAATNRKEMLDSALLRPGRFDRHIQV 243 GG E +QTLNQLL EMDG N+ I+++AATNR ++LD ALLRPGRFDR I V Sbjct: 271 AGLGGGHDEREQTLNQLLVEMDGFSANEG--IIIIAATNRADILDPALLRPGRFDRQITV 328 Query: 244 DMPDKKGRRSILDLHAQNKPLHEEVDLDKIAEEAYGFSGAQLESVMNEAAIYMMRENLTQ 303 D PD GR ++L +HA+NKPL E V+L IA GFSGA LE+++NEAA+ R+N + Sbjct: 329 DRPDVIGREAVLKVHARNKPLDETVNLKSIAMRTPGFSGADLENLLNEAALVAARQNKKK 388 Query: 304 VEQRHLSLAIDKVMMGE-KTDRETNHEEKKRVAIHELGHAIMAELLRPGS-VSQVTLTPR 361 ++ R + A D+V+ G K R + +E+ VA HE GH ++ +L V +VT+ PR Sbjct: 389 IDARDIDEATDRVIAGPAKKSRVISKKERNIVAYHEGGHTVIGLVLDEADMVHKVTIVPR 448 Query: 362 GQALGYVRHNPQTEQYLYTKDYLENQIMIALGGAVAEEMYYGGRSTGSRGDFDQALNIVE 421 GQA GY P+ ++Y TK L ++I+ LGG VAEE+ +G STG+ DF +A NI Sbjct: 449 GQAGGYAVMLPREDRYFQTKPELLDKIVGLLGGRVAEEIIFGEVSTGAHNDFQRATNIAR 508 Query: 422 TMMKSGLTSLGIANLQMVTTE----------------------ELMKENSKILDELMVRT 459 M+ S + LQ ++ E+ +E +I+ E R Sbjct: 509 RMVTEFGMSEKLGPLQFGQSQGGQVFLGRDFNNEQNYSDQIAYEIDQEIQRIIKECYERA 568 Query: 460 HELLGKQRNVFDYSLDILMKEEVLSGEQFR 489 ++L + R+ + L+K E L EQ + Sbjct: 569 KQILTENRDKLELIAQTLLKVETLDAEQIK 598 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.137 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 37,049,629 Number of extensions: 1659375 Number of successful extensions: 5953 Number of sequences better than 1.0e-05: 121 Number of HSP's gapped: 5457 Number of HSP's successfully gapped: 151 Length of query: 500 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 389 Effective length of database: 25,576,357 Effective search space: 9949202873 Effective search space used: 9949202873 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)