BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0543.0022.p (293 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc0244 [I] COG1250 3-hydroxyacyl-CoA dehydrogenase 201 9e-52 CAC2708 [I] COG1250 3-hydroxyacyl-CoA dehydrogenase 191 1e-48 DR1068 [I] COG1250 3-hydroxyacyl-CoA dehydrogenase 188 7e-48 BH3800 [I] COG1250 3-hydroxyacyl-CoA dehydrogenase 184 2e-46 BH1995 [I] COG1250 3-hydroxyacyl-CoA dehydrogenase 172 8e-43 >RSc0244 [I] COG1250 3-hydroxyacyl-CoA dehydrogenase Length = 284 Score = 201 bits (512), Expect = 9e-52, Method: Compositional matrix adjust. Identities = 105/270 (38%), Positives = 156/270 (57%), Gaps = 4/270 (1%) Query: 19 AEMLAAKGLDVMLVEKTAERLDYSYAMIETSLDKQLEKWAITQAEKKLILSRIQKVTHFA 78 A+ A GLDV++V+ + + A + SLD+ ++K +T+A+K L+RI T + Sbjct: 19 AQACAVAGLDVVMVDISEAAVQKGLATVAGSLDRLIKKEKLTEADKAAALARIHGATAYG 78 Query: 79 ELSSCDMVIETITEDLEAKQKVFNQLDQVXXXXGSHIIXXXXXXXXXXXXXXXXXMYPER 138 +L D+VIE TE+L+ K K+ QL+ V H I R Sbjct: 79 DLKRADIVIEAATENLDLKIKILGQLEAVAA---PHAIIASNTSSISITKLAAVLQDASR 135 Query: 139 VIGMHFIHPVAKVDLVEIVRGLKTSDSTFEDTKAFVDEIVEKKGVMIYESPGFVSSRLIC 198 IGMHF +PV + LVEI+RGL+T D+T +A + K + + SPGFV +R++C Sbjct: 136 FIGMHFFNPVPMMALVEIIRGLQTGDATHAAVEALAQRL-GKTPITVKNSPGFVVNRILC 194 Query: 199 LFINEAMHVLQEGVASPEDIDDAMRIGYQFQHGPLEMADRFGLDSVLAALENMFREYGEL 258 INEA L EG+A+PE+ID+ M++G GPL +AD GLD++LA +E + E+G+ Sbjct: 195 PMINEAFCALGEGLATPEEIDEGMKLGCNHPIGPLALADMIGLDTMLAVMEVLHTEFGDP 254 Query: 259 KYRPSTILKKMVRAGQLGMKSGEGFFKYDK 288 KYRP+ +L++MV AG LG KSG G + Y K Sbjct: 255 KYRPAMLLREMVAAGYLGRKSGRGVYDYRK 284 >CAC2708 [I] COG1250 3-hydroxyacyl-CoA dehydrogenase Length = 282 Score = 191 bits (485), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 106/270 (39%), Positives = 152/270 (56%), Gaps = 4/270 (1%) Query: 19 AEMLAAKGLDVMLVEKTAERLDYSYAMIETSLDKQLEKWAITQAEKKLILSRIQKVTHFA 78 A+ AAKG +V+L + E +D I +L K ++K I +A K IL+RI Sbjct: 17 AQAFAAKGFEVVLRDIKDEFVDRGLDFINKNLSKLVKKGKIEEATKVEILTRISGTVDLN 76 Query: 79 ELSSCDMVIETITEDLEAKQKVFNQLDQVXXXXGSHIIXXXXXXXXXXXXXXXXXMYPER 138 + CD+VIE E ++ K+++F LD + I P++ Sbjct: 77 MAADCDLVIEAAVERMDIKKQIFADLDNICK---PETILASNTSSLSITEVASATKRPDK 133 Query: 139 VIGMHFIHPVAKVDLVEIVRGLKTSDSTFEDTKAFVDEIVEKKGVMIYESPGFVSSRLIC 198 VIGMHF +P + LVE++RG+ TS TF+ K + K V + E+PGFV +R++ Sbjct: 134 VIGMHFFNPAPVMKLVEVIRGIATSQETFDAVKE-TSIAIGKDPVEVAEAPGFVVNRILI 192 Query: 199 LFINEAMHVLQEGVASPEDIDDAMRIGYQFQHGPLEMADRFGLDSVLAALENMFREYGEL 258 INEA+ +L EG+AS EDID AM++G GPLE+ D GLD LA ++ ++ E G+ Sbjct: 193 PMINEAVGILAEGIASVEDIDKAMKLGANHPMGPLELGDFIGLDICLAIMDVLYSETGDS 252 Query: 259 KYRPSTILKKMVRAGQLGMKSGEGFFKYDK 288 KYRP T+LKK VRAG LG KSG+GF+ Y K Sbjct: 253 KYRPHTLLKKYVRAGWLGRKSGKGFYDYSK 282 >DR1068 [I] COG1250 3-hydroxyacyl-CoA dehydrogenase Length = 278 Score = 188 bits (478), Expect = 7e-48, Method: Compositional matrix adjust. Identities = 108/271 (39%), Positives = 154/271 (56%), Gaps = 11/271 (4%) Query: 19 AEMLAAKGLDVMLVEKTAERLDYSYAMIETSLDKQLEKWAITQAEKKLILSRIQKVTHFA 78 A++ A G DV++ ++ E LD +IE SL K EK +T A + +L RI T Sbjct: 16 AQVAAQSGFDVVVHDQKQEFLDRGKGVIEKSLGKLHEKGKLTDAPET-VLGRIHFTTDLQ 74 Query: 79 ELSSCDMVIETITEDLEAKQKVFNQLDQVXXXXGSHIIXXXXXXXXXXXXXXXXXMYPER 138 + + CD+V+E I E+ + K +F QL Q+ G I P + Sbjct: 75 DFADCDLVVEAIVENQQIKNDLFKQLGQIVKPEG---ILASNTSSIPITALATASGRPAQ 131 Query: 139 VIGMHFIHPVAKVDLVEIVRGLKTSDSTFEDTKAFVDEIVEKKG---VMIYESPGFVSSR 195 IGMHF++PV + LVE++RG +TSD +T V E EK G + + PGFVS+R Sbjct: 132 FIGMHFMNPVPLMQLVEVIRGYQTSD----ETARIVTETAEKLGKTPLSCNDFPGFVSNR 187 Query: 196 LICLFINEAMHVLQEGVASPEDIDDAMRIGYQFQHGPLEMADRFGLDSVLAALENMFREY 255 ++ +NEA+ + EGVA PE ID M++G GPL +AD GLD+ LA +E + + Sbjct: 188 ILMPMLNEAIQCVMEGVAEPEAIDGIMKLGMNHPMGPLTLADFIGLDTCLAIMEVLHQGL 247 Query: 256 GELKYRPSTILKKMVRAGQLGMKSGEGFFKY 286 G+ KYRPS +L+KMV+AG LG KSGEGF+KY Sbjct: 248 GDDKYRPSPLLRKMVQAGLLGRKSGEGFYKY 278 >BH3800 [I] COG1250 3-hydroxyacyl-CoA dehydrogenase Length = 284 Score = 184 bits (466), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 96/270 (35%), Positives = 151/270 (55%), Gaps = 4/270 (1%) Query: 19 AEMLAAKGLDVMLVEKTAERLDYSYAMIETSLDKQLEKWAITQAEKKLILSRIQKVTHFA 78 A + A G +VML + ++ I +Q+EK +T+ E + LSRI + T Sbjct: 19 AHVHALAGFEVMLYDLDQASIEKGIQTITKQFQRQVEKGRMTEGEMDITLSRIHQSTELQ 78 Query: 79 ELSSCDMVIETITEDLEAKQKVFNQLDQVXXXXGSHIIXXXXXXXXXXXXXXXXXMYPER 138 + D ++E + E++E K +F +LD + + I PE+ Sbjct: 79 DAKKADFIVEAVVENMEVKTNLFAKLDDLAP---ADTILATNTSSLPITEIAAATNRPEK 135 Query: 139 VIGMHFIHPVAKVDLVEIVRGLKTSDSTFEDTKAFVDEIVEKKGVMIYESPGFVSSRLIC 198 VIGMHF++PV + LVEI+RGL T+D +E +A ++ K V + + PGFVS+R++ Sbjct: 136 VIGMHFMNPVPVMKLVEIIRGLATADDVYETVEALTKKL-NKTPVEVNDFPGFVSNRVLM 194 Query: 199 LFINEAMHVLQEGVASPEDIDDAMRIGYQFQHGPLEMADRFGLDSVLAALENMFREYGEL 258 INEA++ + EGVA+PE +D+ M++G GPL +AD GLD+ L +E + +G+ Sbjct: 195 PMINEAIYTVYEGVATPEAVDEVMKLGMNHPMGPLTLADFIGLDTCLYIMETLHEGFGDD 254 Query: 259 KYRPSTILKKMVRAGQLGMKSGEGFFKYDK 288 KYRP +L+K V+AG LG K+G GF+ Y K Sbjct: 255 KYRPCPLLRKYVKAGWLGKKTGRGFYVYHK 284 >BH1995 [I] COG1250 3-hydroxyacyl-CoA dehydrogenase Length = 287 Score = 172 bits (435), Expect = 8e-43, Method: Compositional matrix adjust. Identities = 95/271 (35%), Positives = 157/271 (57%), Gaps = 3/271 (1%) Query: 19 AEMLAAKGLDVMLVEKTAERLDYSYAMIETSLDKQLEKWAITQAEKKLILSRIQKVTHFA 78 A + A GL V+L++ +LD ++ I T ++K + K +++AEK+ L RI+ T + Sbjct: 19 ANLAAMSGLQVVLLDLDDNQLDIAWQKINTFMEKSVAKGKMSEAEKEAALGRIKSTTTYE 78 Query: 79 ELSSCDMVIETITEDLEAKQKVFNQLDQVXXXXGSHIIXXXXXXXXXXXXXXXXXMYPER 138 EL+ D+VIE + E+L+ K++VF+ LD + I P+R Sbjct: 79 ELAEADLVIEAVIENLDVKKEVFHTLDTCL---ANDTIIATNTSSMSITEIAAATNRPDR 135 Query: 139 VIGMHFIHPVAKVDLVEIVRGLKTSDSTFEDTKAFVDEIVEKKGVMIYESPGFVSSRLIC 198 V+GMHF +P + LVE+VRG +TSD T E K F ++ ++ + ++PGF+ +R++ Sbjct: 136 VVGMHFFNPAQLMKLVEVVRGYQTSDDTVETVKQFARQLKKEPIEVKKDTPGFIVNRIMI 195 Query: 199 LFINEAMHVLQEGVASPEDIDDAMRIGYQFQHGPLEMADRFGLDSVLAALENMFREYGEL 258 EA+ +L+EGVAS EDID A+ +G + GP + D G+D L +E +E+ + Sbjct: 196 PQFIEAIKLLEEGVASAEDIDKAVTLGLNYPMGPFTLQDYAGVDIGLHVMEYFQQEFNDP 255 Query: 259 KYRPSTILKKMVRAGQLGMKSGEGFFKYDKD 289 ++ P +LK++VRAG+LG K G GF+ YD + Sbjct: 256 RFTPPLLLKQLVRAGRLGKKVGAGFYDYDNE 286 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.136 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,836,171 Number of extensions: 664955 Number of successful extensions: 2190 Number of sequences better than 1.0e-05: 82 Number of HSP's gapped: 2006 Number of HSP's successfully gapped: 99 Length of query: 293 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 187 Effective length of database: 26,153,027 Effective search space: 4890616049 Effective search space used: 4890616049 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)