BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0543.0021.p (402 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value L73818 [C] COG0282 Acetate kinase 394 e-109 lin1616 [C] COG0282 Acetate kinase 393 e-109 ML0224 [H] COG0294 Dihydropteroate synthase and related enzymes 388 e-107 L0224 [C] COG0282 Acetate kinase 388 e-107 lin1132 [C] COG0282 Acetate kinase 371 e-102 >L73818 [C] COG0282 Acetate kinase Length = 395 Score = 394 bits (1012), Expect = e-109, Method: Compositional matrix adjust. Identities = 204/391 (52%), Positives = 271/391 (69%), Gaps = 8/391 (2%) Query: 4 LVINSGSSSLKYQLYNMTDESVLAKGLVERIGMDSSILNHKPTGKQEVTEVSEILEHNTA 63 L +N+GSSS+K+Q+Y M +E VLAKGL+ERIG+ SI+ K G+Q+ V +I H A Sbjct: 5 LAVNAGSSSMKWQMYEMPEEKVLAKGLIERIGLKDSIVTVK-FGEQKEERVFDIPNHTEA 63 Query: 64 IRKVLACLTDKEHGVISSIDEINAVGHRVVHGGEFFKASALVDADAKTKIRQLFDLAPLH 123 + +L L K ++ EI VGHRVV GGE F+ S +V + +++ L LAPLH Sbjct: 64 VEVLLEDL--KRLKIVEEFTEITGVGHRVVAGGEIFQKSTVVTPEVLQQVKDLSALAPLH 121 Query: 124 NPAAVMGISATEVNMPGVPQVVVFDTAFHQTMPEKAYMYAIPRVLYNKYKVRRYGAHGTS 183 NPA GI A +P VVVFDTAFH TMPEKA+ Y +P+ Y Y VR+YGAHGTS Sbjct: 122 NPANAAGIEAFLNLLPDATSVVVFDTAFHTTMPEKAFRYPLPKKYYTDYAVRKYGAHGTS 181 Query: 184 HDFVSKAAADYLERPLEDLKIITCHIGNGASVTAVDGGQSIDTSMGMTPLEGLMMGTRSG 243 H +VS+ AA L +P+E+ KIIT HIGNGAS+TAV GG+SIDTSMG TPL G+MMGTR+G Sbjct: 182 HMYVSQEAAKLLGKPIEETKIITAHIGNGASLTAVKGGKSIDTSMGFTPLAGVMMGTRTG 241 Query: 244 DLDPAIVPYVM-NKEELSVGE-VNSMLNKHSGLLAISGVSSDMRDIIDGAEKGEPNSTLA 301 ++DP++ PY++ N EL + V M+NK SGL +SG+SSDMRDII A+ + ++ LA Sbjct: 242 EMDPSVFPYLIENDSELKDAQGVVDMMNKESGLYGVSGISSDMRDII-AAKDTDTDAKLA 300 Query: 302 FDMYEYRLRKYIGSYAAAMNGVDVIVFTAGVGENAAVLRAKVLNNLTFLGIELDAEANKV 361 F+MY R++K+IG Y A +NG D +VFTAG+GEN+ +R +L+ LT+ GIE+D E N Sbjct: 301 FEMYVDRIQKFIGQYLAVLNGADALVFTAGIGENSVPVREAILSGLTWFGIEVDPEKNVF 360 Query: 362 RSGDPRRISTVESKVQVLVVPTNEELVIARD 392 G IST S+V+V V+PT+EELVIARD Sbjct: 361 --GVEGEISTPHSRVKVFVIPTDEELVIARD 389 >lin1616 [C] COG0282 Acetate kinase Length = 397 Score = 393 bits (1010), Expect = e-109, Method: Compositional matrix adjust. Identities = 201/395 (50%), Positives = 277/395 (70%), Gaps = 4/395 (1%) Query: 4 LVINSGSSSLKYQLYNMTDESVLAKGLVERIGMDSSILNHKPTGKQEVTEVSEILEHNTA 63 + IN+GSSSLK+QLY+M E V+ G+VERIG+ SI G +++ E+ +I +H A Sbjct: 5 IAINAGSSSLKFQLYDMPSERVITAGIVERIGLKDSIFTITVDG-EKIKEIIDIPDHEIA 63 Query: 64 IRKVLACLTDKEHGVISSIDEINAVGHRVVHGGEFFKASALVDADAKTKIRQLFDLAPLH 123 ++ +L L + H VI S DEI +GHRVVHGGE F S +D I L +LAPLH Sbjct: 64 VQMLLEKLIN--HKVIGSYDEITGIGHRVVHGGERFPESVYIDDQVIKDIEALSELAPLH 121 Query: 124 NPAAVMGISATEVNMPGVPQVVVFDTAFHQTMPEKAYMYAIPRVLYNKYKVRRYGAHGTS 183 NPA V GI A +P V V VFDTAFHQTMP +Y+Y++P Y Y +R+YG HGTS Sbjct: 122 NPANVTGIKAFRKILPDVVSVAVFDTAFHQTMPPASYLYSLPYSYYEDYGIRKYGFHGTS 181 Query: 184 HDFVSKAAADYLERPLEDLKIITCHIGNGASVTAVDGGQSIDTSMGMTPLEGLMMGTRSG 243 H +VS+ AA+ L RP+E+L+++TCH+GNGAS+ A++GG+S+DTSMG TPL G+ MGTRSG Sbjct: 182 HKYVSERAAELLGRPVEELRLLTCHLGNGASIAAIEGGKSMDTSMGFTPLAGVSMGTRSG 241 Query: 244 DLDPAIVPYVMNKEELSVGEVNSMLNKHSGLLAISGVSSDMRDIIDGAEKGEPNSTLAFD 303 ++DPA++P++M K + +V +LNK SG+L +SG+SSD+RD+ D A KG + LA Sbjct: 242 NIDPALIPFIMEKTGKTAEQVLDVLNKESGMLGVSGISSDLRDLEDEAAKGNDRAELALQ 301 Query: 304 MYEYRLRKYIGSYAAAMNGVDVIVFTAGVGENAAVLRAKVLNNLTFLGIELDAEANKVRS 363 ++ R+ KYIGSYAA MNGVD I+FTAG+GEN++ +R KVL L F+G+ D N+VR Sbjct: 302 VFVDRIHKYIGSYAARMNGVDAIIFTAGIGENSSYIREKVLRGLEFMGVYWDPALNQVR- 360 Query: 364 GDPRRISTVESKVQVLVVPTNEELVIARDTYRIVQ 398 G+ R ++ S V+V+++PTNEEL+IARD I Q Sbjct: 361 GEERFLNYPHSPVKVIIIPTNEELMIARDVETIKQ 395 >ML0224 [H] COG0294 Dihydropteroate synthase and related enzymes Length = 395 Score = 388 bits (996), Expect = e-107, Method: Compositional matrix adjust. Identities = 196/391 (50%), Positives = 269/391 (68%), Gaps = 7/391 (1%) Query: 4 LVINSGSSSLKYQLYNMTDESVLAKGLVERIGMDSSILNHKPTGKQEVTEVSEILEHNTA 63 L +N+GSSSLK+QLY M +E+V+AKG+ ERIG+ SI K ++ + EI++H A Sbjct: 5 LAVNAGSSSLKWQLYEMPEETVIAKGIFERIGLSGSISTTKFNNEEHFRK-QEIVDHRQA 63 Query: 64 IRKVLACLTDKEHGVISSIDEINAVGHRVVHGGEFFKASALVDADAKTKIRQLFDLAPLH 123 + ++ L +I EI +GHRVV GGEFFK S ++ + +I+ L LAPLH Sbjct: 64 VLLLMDELI--HFKLIHEFREITGIGHRVVAGGEFFKTSTVISPEVLAEIKNLSTLAPLH 121 Query: 124 NPAAVMGISATEVNMPGVPQVVVFDTAFHQTMPEKAYMYAIPRVLYNKYKVRRYGAHGTS 183 NPA V+GI A + +P V VFDTAFH T+PEKAY Y IP Y Y +R+YGAHGTS Sbjct: 122 NPANVLGIEAFQRLLPDALAVAVFDTAFHSTLPEKAYRYPIPTKYYEDYSIRKYGAHGTS 181 Query: 184 HDFVSKAAADYLERPLEDLKIITCHIGNGASVTAVDGGQSIDTSMGMTPLEGLMMGTRSG 243 H +V++ A L +PLEDLK+IT HIGNGAS+TA++ G+S+DTSMG TPL G+MMGTR+G Sbjct: 182 HMYVAQEAEKVLGKPLEDLKLITAHIGNGASITAIEAGKSVDTSMGFTPLAGVMMGTRAG 241 Query: 244 DLDPAIVPYVMNKEE--LSVGEVNSMLNKHSGLLAISGVSSDMRDIIDGAEKGEPNSTLA 301 ++D +++PY++ + + +V +LNK SG+L +S +SSDMRD+ + KG P + LA Sbjct: 242 EMDASVIPYMLESDPSLRNAQDVIDILNKDSGVLGVSELSSDMRDLSEAVAKGNPKAILA 301 Query: 302 FDMYEYRLRKYIGSYAAAMNGVDVIVFTAGVGENAAVLRAKVLNNLTFLGIELDAEANKV 361 ++MY RL+K+I Y +NG D ++FTAGVGEN +R V+N L++ G+E+D E+ V Sbjct: 302 YEMYVDRLKKFIAQYFGVLNGADALIFTAGVGENDTAVRTDVVNGLSWFGMEID-ESKNV 360 Query: 362 RSGDPRRISTVESKVQVLVVPTNEELVIARD 392 R G IS ESKV+VLVVPTNEELVIARD Sbjct: 361 R-GAFGIISKPESKVKVLVVPTNEELVIARD 390 >L0224 [C] COG0282 Acetate kinase Length = 395 Score = 388 bits (996), Expect = e-107, Method: Compositional matrix adjust. Identities = 196/391 (50%), Positives = 269/391 (68%), Gaps = 7/391 (1%) Query: 4 LVINSGSSSLKYQLYNMTDESVLAKGLVERIGMDSSILNHKPTGKQEVTEVSEILEHNTA 63 L +N+GSSSLK+QLY M +E+V+AKG+ ERIG+ SI K ++ + EI++H A Sbjct: 5 LAVNAGSSSLKWQLYEMPEETVIAKGIFERIGLSGSISTTKFNNEEHFRK-QEIVDHRQA 63 Query: 64 IRKVLACLTDKEHGVISSIDEINAVGHRVVHGGEFFKASALVDADAKTKIRQLFDLAPLH 123 + ++ L +I EI +GHRVV GGEFFK S ++ + +I+ L LAPLH Sbjct: 64 VLLLMDELI--HFKLIHEFREITGIGHRVVAGGEFFKTSTVISPEVLAEIKNLSTLAPLH 121 Query: 124 NPAAVMGISATEVNMPGVPQVVVFDTAFHQTMPEKAYMYAIPRVLYNKYKVRRYGAHGTS 183 NPA V+GI A + +P V VFDTAFH T+PEKAY Y IP Y Y +R+YGAHGTS Sbjct: 122 NPANVLGIEAFQRLLPDALAVAVFDTAFHSTLPEKAYRYPIPTKYYEDYSIRKYGAHGTS 181 Query: 184 HDFVSKAAADYLERPLEDLKIITCHIGNGASVTAVDGGQSIDTSMGMTPLEGLMMGTRSG 243 H +V++ A L +PLEDLK+IT HIGNGAS+TA++ G+S+DTSMG TPL G+MMGTR+G Sbjct: 182 HMYVAQEAEKVLGKPLEDLKLITAHIGNGASITAIEAGKSVDTSMGFTPLAGVMMGTRAG 241 Query: 244 DLDPAIVPYVMNKEE--LSVGEVNSMLNKHSGLLAISGVSSDMRDIIDGAEKGEPNSTLA 301 ++D +++PY++ + + +V +LNK SG+L +S +SSDMRD+ + KG P + LA Sbjct: 242 EMDASVIPYMLESDPSLRNAQDVIDILNKDSGVLGVSELSSDMRDLSEAVAKGNPKAILA 301 Query: 302 FDMYEYRLRKYIGSYAAAMNGVDVIVFTAGVGENAAVLRAKVLNNLTFLGIELDAEANKV 361 ++MY RL+K+I Y +NG D ++FTAGVGEN +R V+N L++ G+E+D E+ V Sbjct: 302 YEMYVDRLKKFIAQYFGVLNGADALIFTAGVGENDTAVRTDVVNGLSWFGMEID-ESKNV 360 Query: 362 RSGDPRRISTVESKVQVLVVPTNEELVIARD 392 R G IS ESKV+VLVVPTNEELVIARD Sbjct: 361 R-GAFGIISKPESKVKVLVVPTNEELVIARD 390 >lin1132 [C] COG0282 Acetate kinase Length = 397 Score = 371 bits (953), Expect = e-102, Method: Compositional matrix adjust. Identities = 186/393 (47%), Positives = 263/393 (66%), Gaps = 6/393 (1%) Query: 3 ILVINSGSSSLKYQLYNMTDESVLAKGLVERIGMDSSILNHKPTGKQEVTEVSEILEHNT 62 I+ IN+GSSSLK+Q++ M E VL KGL+ERIG+ +I N +++ E I H Sbjct: 4 IMAINAGSSSLKFQIFTMPGEEVLVKGLIERIGLPDAIFN-MSFQNEKIKETRAINNHGE 62 Query: 63 AIRKVLACLTDKEHGVISSIDEINAVGHRVVHGGEFFKASALVDADAKTKIRQLFDLAPL 122 A+ +L L K H VI+ ++EI VGHRV HGGE F +S +V D I + LAPL Sbjct: 63 AVEILLEQL--KTHQVINDLNEITGVGHRVAHGGEAFVSSCIVTDDVVKGIEDVTSLAPL 120 Query: 123 HNPAAVMGISATEVNMPGVPQVVVFDTAFHQTMPEKAYMYAIPRVLYNKYKVRRYGAHGT 182 HNPA ++GI +P V VFDTAFHQT+PE+ ++YA+P LY K+ +R+YG HGT Sbjct: 121 HNPANIIGIKTFRELLPNAVSVAVFDTAFHQTIPEENFLYALPYELYEKHHIRKYGFHGT 180 Query: 183 SHDFVSKAAADYLERPLEDLKIITCHIGNGASVTAVDGGQSIDTSMGMTPLEGLMMGTRS 242 SH +V+ AA+ L++PLE LKII+CH+GNGASV A++ G+S++TSMG TP GLMMGTRS Sbjct: 181 SHKYVAGKAAEVLKKPLEKLKIISCHLGNGASVCAIEAGKSVNTSMGFTPNAGLMMGTRS 240 Query: 243 GDLDPAIVPYVMNKEELSVGEVNSMLNKHSGLLAISGVSSDMRDIIDGAEKGEPNSTLAF 302 G +D I+PY++++ S+ EV M++ SG+L +SG+SSD RDI A +G + L Sbjct: 241 GTIDATIIPYLVDELGYSLDEVTHMMSSESGVLGVSGISSDFRDIEIAANEGNSRALLTL 300 Query: 303 DMYEYRLRKYIGSYAAAMNGVDVIVFTAGVGENAAVLRAKVLNNLTFLGIELDAEANKVR 362 M+ ++ YIG+YA+AMNG D ++FTAGVGEN+ ++R V L++LG+ + N Sbjct: 301 RMFTGQICNYIGAYASAMNGCDALLFTAGVGENSPLIRKMVTEQLSYLGVTCNVTKN--N 358 Query: 363 SGDPRRISTVESKVQVLVVPTNEELVIARDTYR 395 +GD IS + V+V ++PTNEEL+IARD + Sbjct: 359 AGD-MIISNDDEAVKVCIIPTNEELMIARDVEK 390 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.133 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,566,134 Number of extensions: 1156560 Number of successful extensions: 2953 Number of sequences better than 1.0e-05: 27 Number of HSP's gapped: 2888 Number of HSP's successfully gapped: 27 Length of query: 402 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 293 Effective length of database: 25,807,025 Effective search space: 7561458325 Effective search space used: 7561458325 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 117 (49.7 bits)