BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0543.0020.p (431 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPy0651 [J] COG0017 Aspartyl/asparaginyl-tRNA synthetases 478 e-135 DR1270 [J] COG0017 Aspartyl/asparaginyl-tRNA synthetases 473 e-133 Ta0519 [J] COG0017 Aspartyl/asparaginyl-tRNA synthetases 396 e-110 PAB2203 [J] COG0017 Aspartyl/asparaginyl-tRNA synthetases 393 e-109 TVN0996 [J] COG0017 Aspartyl/asparaginyl-tRNA synthetases 392 e-109 >SPy0651 [J] COG0017 Aspartyl/asparaginyl-tRNA synthetases Length = 346 Score = 478 bits (1231), Expect = e-135, Method: Compositional matrix adjust. Identities = 0/448 (0%), Positives = 0/448 (0%), Gaps = 17/448 (3%) Query: 1 MANKSV-IKNVNEHVGEAVVIGCWVNNKRSSGKIQFLQLRDGTGYIQGVVVKSEVPEQV- 58 M + ++ + N + Q + V VP + Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 59 -------WDDAKSLTQESSLYVTGIIREEPRSQSGYEMTVTGIEVLHLTENYPITPKEHG 111 + S + + S++ + + + Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 112 VDFLMDHRHLWLRSAKQRAVLVIRAEIIRAVQQFFNENGFTKVDPPILTPTSAEGTTNLF 171 + + + + Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 172 HTKYFEEDAYLTQSGQLYMEAAAMALGRVYSFGPTFRAEKSKTRRHLIEFWMIEPEMAFT 231 T + + ++ +E T Sbjct: 181 ETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGT 240 Query: 232 DHEESLRVQEEFISFVVQSVLKNCRAELEAVGRDISKLEN-IKAPFPRITYDDAIKFLNE 290 + L + + + + + ++ + A Sbjct: 241 LTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQP 300 Query: 291 ------KGYE-IAWGEDFGAPHETAIEKANDKPVFITHYPASFKAFYMKPHPERPEVVLC 343 G Sbjct: 301 FVASTLLGATT 360 Query: 344 ADMIAPEGYGEIIGGSQRIDDPALLEERFKEHNLSMDAYKWYMDLRTYGSVPHSGFGLGL 403 Sbjct: 361 420 Query: 404 ERTVAWICGLDHVRETIPFPRTLYRLYP 431 Sbjct: 421 448 >DR1270 [J] COG0017 Aspartyl/asparaginyl-tRNA synthetases Length = 346 Score = 473 bits (1218), Expect = e-133, Method: Compositional matrix adjust. Identities = 0/447 (0%), Positives = 0/447 (0%), Gaps = 20/447 (4%) Query: 3 NKSVIKNVNEHVGEAVVIGCWVNNKRSSGKIQFLQLRDGTGYIQGVVVKSEVPEQVWDDA 62 + S+ + + + Q + + V Q + Sbjct: 8 HSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTET 67 Query: 63 KSLTQESSLYVTGIIREEPRSQSGYEMTVTGIEVLHLTEN---YPITPKEHGVDFLMDHR 119 + + + GI + L Sbjct: 68 YVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD--QALDRKNIREALHDSLKRLQTDY 125 Query: 120 HLWLRSAKQRAVLVIRAEIIRAVQQFFNENGFTKVDPPILTPTSAEGTTNLFHTKYFEED 179 + + ++ + + A + + Sbjct: 126 LDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFG 185 Query: 180 -AYLTQSGQLYMEAAAMALGRVYSFGPTFRAEKSKTRRHLIEFWMIEPEMAFTDHEESLR 238 + + + YS ++ Sbjct: 186 VMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKY 245 Query: 239 VQEEFISFVVQSVLKNCRAELEAVGRDISKLENI-KAPFPRITYDDAIKFL--------- 288 + + ++ + A+ F+ Sbjct: 246 LNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVAST 305 Query: 289 ----NEKGYEIAWGEDFGAPHETAIEKANDKPVFITHYPASFKAFYMKPHPERPEVVLCA 344 Sbjct: 306 LLGA 365 Query: 345 DMIAPEGYGEIIGGSQRIDDPALLEERFKEHNLSMDAYKWYMDLRTYGSVPHSGFGLGLE 404 Sbjct: 366 425 Query: 405 RTVAWICGLDHVRETIPFPRTLYRLYP 431 Sbjct: 426 452 >Ta0519 [J] COG0017 Aspartyl/asparaginyl-tRNA synthetases Length = 346 Score = 396 bits (1018), Expect = e-110, Method: Compositional matrix adjust. Identities = 0/419 (0%), Positives = 0/419 (0%), Gaps = 1/419 (0%) Query: 13 HVGEAVVIGCWVNNKRSSGKIQFLQLRDGTGYIQGVVVKSEVPEQVWDDAKSLTQESSLY 72 V + + S I + V P + + LT+ Sbjct: 12 EVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGN 71 Query: 73 VTGIIREEPRSQSGYEMTVTGIEVLHLTENYPITPKEHGVDFLMDHRHLWLRSAKQRAVL 132 + +++ + + L++ Sbjct: 72 WLAKHGSREKLIIASKVSGPSRNNDKGIRPDQAL-DRKNIREALHDSLKRLQTDYLDLYQ 130 Query: 133 VIRAEIIRAVQQFFNENGFTKVDPPILTPTSAEGTTNLFHTKYFEEDAYLTQSGQLYMEA 192 V + + L T K + + Sbjct: 131 VHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYL 190 Query: 193 AAMALGRVYSFGPTFRAEKSKTRRHLIEFWMIEPEMAFTDHEESLRVQEEFISFVVQSVL 252 + R + + S + Sbjct: 191 HLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAK 250 Query: 253 KNCRAELEAVGRDISKLENIKAPFPRITYDDAIKFLNEKGYEIAWGEDFGAPHETAIEKA 312 E + L+ +A+ T + Sbjct: 251 PAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGAT 310 Query: 313 NDKPVFITHYPASFKAFYMKPHPERPEVVLCADMIAPEGYGEIIGGSQRIDDPALLEERF 372 Sbjct: 311 370 Query: 373 KEHNLSMDAYKWYMDLRTYGSVPHSGFGLGLERTVAWICGLDHVRETIPFPRTLYRLYP 431 Sbjct: 371 429 >PAB2203 [J] COG0017 Aspartyl/asparaginyl-tRNA synthetases Length = 346 Score = 393 bits (1009), Expect = e-109, Method: Compositional matrix adjust. Identities = 0/434 (0%), Positives = 0/434 (0%), Gaps = 3/434 (0%) Query: 1 MANKSVIKNVNEHV-GEAVVIGCWVNNKRSSGKIQFLQLRDGTGYIQGVVVKSEVPEQVW 59 M + + E + N + Q + V VP + Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 60 DDAKSLTQESSLYVTGIIREEPRSQSGYEMTVTGIEVLHLTENYPI--TPKEHGVDFLMD 117 + T + RE+ S + + +E D L Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 118 HRHLWLRSAKQRAVLVIRAEIIRAVQQFFNENGFTKVDPPILTPTSAEGTTNLFHTKYFE 177 + +L + + + + + + + + + Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 178 EDAYLTQSGQLYMEAAAMALGRVYSFGPTFRAEKSKTRRHLIEFWMIEPEMAFTDHEESL 237 E A+ + + + + + + +A++ Sbjct: 181 ETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGT 240 Query: 238 RVQEEFISFVVQSVLKNCRAELEAVGRDISKLENIKAPFPRITYDDAIKFLNEKGYEIAW 297 + + + ++ + + Sbjct: 241 LTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQP 300 Query: 298 GEDFGAPHETAIEKANDKPVFITHYPASFKAFYMKPHPERPEVVLCADMIAPEGYGEIIG 357 Sbjct: 301 360 Query: 358 GSQRIDDPALLEERFKEHNLSMDAYKWYMDLRTYGSVPHSGFGLGLERTVAWICGLDHVR 417 Sbjct: 361 420 Query: 418 ETIPFPRTLYRLYP 431 Sbjct: 421 434 >TVN0996 [J] COG0017 Aspartyl/asparaginyl-tRNA synthetases Length = 346 Score = 392 bits (1008), Expect = e-109, Method: Compositional matrix adjust. Identities = 0/419 (0%), Positives = 0/419 (0%), Gaps = 1/419 (0%) Query: 13 HVGEAVVIGCWVNNKRSSGKIQFLQLRDGTGYIQGVVVKSEVPEQVWDDAKSLTQESSLY 72 V + + S I + V P + + LT+ Sbjct: 12 EVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGN 71 Query: 73 VTGIIREEPRSQSGYEMTVTGIEVLHLTENYPITPKEHGVDFLMDHRHLWLRSAKQRAVL 132 + +++ + + L++ Sbjct: 72 WLAKHGSREKLIIASKVSGPSRNNDKGIRPDQAL-DRKNIREALHDSLKRLQTDYLDLYQ 130 Query: 133 VIRAEIIRAVQQFFNENGFTKVDPPILTPTSAEGTTNLFHTKYFEEDAYLTQSGQLYMEA 192 V + + L T K + + Sbjct: 131 VHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYL 190 Query: 193 AAMALGRVYSFGPTFRAEKSKTRRHLIEFWMIEPEMAFTDHEESLRVQEEFISFVVQSVL 252 + R + + S + Sbjct: 191 HLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAK 250 Query: 253 KNCRAELEAVGRDISKLENIKAPFPRITYDDAIKFLNEKGYEIAWGEDFGAPHETAIEKA 312 E + L+ +A+ T + Sbjct: 251 PAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGAT 310 Query: 313 NDKPVFITHYPASFKAFYMKPHPERPEVVLCADMIAPEGYGEIIGGSQRIDDPALLEERF 372 Sbjct: 311 370 Query: 373 KEHNLSMDAYKWYMDLRTYGSVPHSGFGLGLERTVAWICGLDHVRETIPFPRTLYRLYP 431 Sbjct: 371 429 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 33,319,037 Number of extensions: 1426036 Number of successful extensions: 3720 Number of sequences better than 1.0e-05: 50 Number of HSP's gapped: 3606 Number of HSP's successfully gapped: 51 Length of query: 431 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 322 Effective length of database: 25,807,025 Effective search space: 8309862050 Effective search space used: 8309862050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)